Invitrogen

CD117 (c-Kit) Monoclonal Antibody (ACK2), APC-eFluor™ 780, eBioscience™

CD117 (c-Kit) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 20

CD117 (c-Kit) Antibody (47-1172-82) in Flow

C57BL/6 mouse bone marrow cells were stained with Mouse Hematopoietic Lineage Antibody Cocktail, eFluor 450 (Product # 88-7772-72), Sca-1 Monoclonal Antibody, FITC (Product # 11-5981-82), and 0.06 µg of Rat IgG2b kappa Isotype Control, APC-eFluor 780 (Product # 47-4031-83) (left) or 0.06 µg of CD117 (c-Kit) Monoclonal Antibody, APC-eFluor 780 (right). Cells in the Lineage low/negative gate were used for analysis.

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Product Details

47-1172-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 29 publications 29 publications

Product Specifications

Species Reactivity

Mouse

Published species

Hamster, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ACK2

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1582226

Product Specific Information

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Applications Reported: This ACK2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Engineered bone marrow as a clinically relevant ex vivo model for primary bone cancer research and drug screening.

Proceedings of the National Academy of Sciences of the United States of America

Griffin KH,Thorpe SW,Sebastian A,Hum NR,Coonan TP,Sagheb IS,Loots GG,Randall RL,Leach JK

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 47-1172-82) in Flow Cytometry

Tue Sep 26 00:00:00 EDT 2023

Landscape of mast cell populations across organs in mice and humans.

The Journal of experimental medicine

Tauber M,Basso L,Martin J,Bostan L,Pinto MM,Thierry GR,Houmadi R,Serhan N,Loste A,Blériot C,Kamphuis JBJ,Grujic M,Kjellén L,Pejler G,Paul C,Dong X,Galli SJ,Reber LL,Ginhoux F,Bajenoff M,Gentek R,Gaudenzio N

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 47-1172-82) in Flow Cytometry

Mon Oct 02 00:00:00 EDT 2023

FANCA deficiency promotes leukaemic progression by allowing the emergence of cells carrying oncogenic driver mutations.

Oncogene

Pawlikowska P,Delestré L,Gregoricchio S,Oppezzo A,Esposito M,Diop MB,Rosselli F,Guillouf C

47-1172-82 was used in Flow cytometry/Cell sorting to indicate that loss of the FANC pathway, known to control genetic instability, fosters the expansion of leukaemic cells carrying oncogenic mutations rather than mutation formation.

Fri Sep 01 00:00:00 EDT 2023

The AML-associated K313 mutation enhances C/EBPα activity by leading to C/EBPα overexpression.

Cell death & disease

Gentle IE,Moelter I,Badr MT,Döhner K,Lübbert M,Häcker G

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 47-1172-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

TNF-α-induced alterations in stromal progenitors enhance leukemic stem cell growth via CXCR2 signaling.

Cell reports

Agarwal P,Li H,Choi K,Hueneman K,He J,Welner RS,Starczynowski DT,Bhatia R

47-1172-82 was used in Flow Cytometry to find that TNF-α-mediated alterations in CML BM stromal niches enhance support of LSC maintenance and growth via CXCL1-CXCR2 signaling and that CXCR2 inhibition effectively depletes CML LSCs.

Tue Jul 13 00:00:00 EDT 2021

The lifespan quantitative trait locus gene Securin controls hematopoietic progenitor cell function.

Haematologica

Brown A,Schuetz D,Han Y,Daria D,Nattamai KJ,Eiwen K,Sakk V,Pospiech J,Saller T,van Zant G,Wagner W,Geiger H

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 47-1172-82) in Flow Cytometry

Tue Apr 27 00:00:00 EDT 2021

Radiation causes tissue damage by dysregulating inflammasome-gasdermin D signaling in both host and transplanted cells.

PLoS biology

Xiao J,Wang C,Yao JC,Alippe Y,Yang T,Kress D,Sun K,Kostecki KL,Monahan JB,Veis DJ,Abu-Amer Y,Link DC,Mbalaviele G

47-1172 was used in Flow cytometry/Cell sorting to test the hypothesis that lethal radiation damages tissues, thereby unleashing signals that indiscriminately activate the inflammasome pathways in host and transplanted cells.

Sat Aug 01 00:00:00 EDT 2020

Development of an inexpensive Raman-compatible substrate for the construction of a microarray screening platform.

The Analyst

Pastrana-Otero I,Majumdar S,Gilchrist AE,Gorman BL,Harley BAC,Kraft ML

47-1172-82 was used in Flow Cytometry to show that the spectra acquired from individual living cells on functionalized PA gels attached to our custom substrates were of sufficient quality to enable accurate identification of cell phenotypes using PLS-DA models of the cell spectra.

Mon Oct 26 00:00:00 EDT 2020

Connecting secretome to hematopoietic stem cell phenotype shifts in an engineered bone marrow niche.

Integrative biology : quantitative biosciences from nano to macro

Gilchrist AE,Harley BAC

47-1172-82 was used in Flow Cytometry to investigate the mechanism of how marrow-derived mesenchymal stromal and progenitor cells impacts hematopoietic stem cells fate decisions.

Fri Jul 10 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 47-1172-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Loss of FGFR3 Delays Acute Myeloid Leukemogenesis by Programming Weakly Pathogenic CD117-Positive Leukemia Stem-Like Cells.

Frontiers in pharmacology

Guo C,Ran Q,Sun C,Zhou T,Yang X,Zhang J,Pang S,Xiao Y

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 47-1172-82) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

Osterix-Cre marks distinct subsets of CD45- and CD45+ stromal populations in extra-skeletal tumors with pro-tumorigenic characteristics.

eLife

Ricci B,Tycksen E,Celik H,Belle JI,Fontana F,Civitelli R,Faccio R

47-1172 was used in Flow cytometry/Cell sorting to find Osx transcript and Osx protein expression early during hematopoiesis, in subsets of hematopoietic stem cells and multipotent progenitor populations.

Wed Aug 05 00:00:00 EDT 2020

Transcriptional Basis of Mouse and Human Dendritic Cell Heterogeneity.

Cell

Brown CC,Gudjonson H,Pritykin Y,Deep D,Lavallée VP,Mendoza A,Fromme R,Mazutis L,Ariyan C,Leslie C,Pe'er D,Rudensky AY

47-1172 was used in Flow cytometry/Cell sorting to identify two principal cDC2 lineages defined by distinct developmental pathways and transcriptional regulators, including T-bet and RORγt, two key transcription factors known to define innate and adaptive lymphocyte subsets.

Thu Oct 31 00:00:00 EDT 2019

Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells.

Cell stem cell

Agarwal P,Isringhausen S,Li H,Paterson AJ,He J,Gomariz Á,Nagasawa T,Nombela-Arrieta C,Bhatia R

47-1172-82 was used in Flow Cytometry to highlight a critical role of niche-specific effects of Cxcl12 expression in maintaining quiescence of TKI-resistant LSC populations.

Thu May 02 00:00:00 EDT 2019

SIRT1 regulates metabolism and leukemogenic potential in CML stem cells.

The Journal of clinical investigation

Abraham A,Qiu S,Chacko BK,Li H,Paterson A,He J,Agarwal P,Shah M,Welner R,Darley-Usmar VM,Bhatia R

47-1172 was used in Flow cytometry/Cell sorting to demonstrate an important role for SIRT1 in leukemia development.

Mon Jun 10 00:00:00 EDT 2019

Bone marrow niche trafficking of miR-126 controls the self-renewal of leukemia stem cells in chronic myelogenous leukemia.

Nature medicine

Zhang B,Nguyen LXT,Li L,Zhao D,Kumar B,Wu H,Lin A,Pellicano F,Hopcroft L,Su YL,Copland M,Holyoake TL,Kuo CJ,Bhatia R,Snyder DS,Ali H,Stein AS,Brewer C,Wang H,McDonald T,Swiderski P,Troadec E,Chen CC,Dorrance A,Pullarkat V,Yuan YC,Perrotti D,Carlesso N,Forman SJ,Kortylewski M,Kuo YH,Marcucci G

47-1172 was used in Flow cytometry/Cell sorting to demonstrate how bone marrow endothelial cells supply miR-126 to leukemia stem cells in chronic myelogenous leukemia which modulates their quiescence and self-renewal.

Tue May 01 00:00:00 EDT 2018

CD150^high Bone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine.

Cell stem cell

Hirata Y,Furuhashi K,Ishii H,Li HW,Pinho S,Ding L,Robson SC,Frenette PS,Fujisaki J

47-1172-82 was used in Flow Cytometry to describe a Treg subpopulation that controls hematopoietic stem cell (HSC) quiescence and engraftment.

Thu Mar 01 00:00:00 EST 2018

The E-Id Protein Axis Specifies Adaptive Lymphoid Cell Identity and Suppresses Thymic Innate Lymphoid Cell Development.

Immunity

Miyazaki M,Miyazaki K,Chen K,Jin Y,Turner J,Moore AJ,Saito R,Yoshida K,Ogawa S,Rodewald HR,Lin YC,Kawamoto H,Murre C

47-1172 was used in Flow cytometry/Cell sorting to propose that the E-Id protein axis specifies innate and adaptive lymphoid cell fate.

Tue May 16 00:00:00 EDT 2017

GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis.

The Journal of clinical investigation

Géraud C,Koch PS,Zierow J,Klapproth K,Busch K,Olsavszky V,Leibing T,Demory A,Ulbrich F,Diett M,Singh S,Sticht C,Breitkopf-Heinlein K,Richter K,Karppinen SM,Pihlajaniemi T,Arnold B,Rodewald HR,Augustin HG,Schledzewski K,Goerdt S

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 47-1172-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Low Testosterone Alters the Activity of Mouse Prostate Stem Cells.

The Prostate

Zhou Y,Copeland B,Otto-Duessel M,He M,Markel S,Synold TW,Jones JO

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 47-1172-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

A Functional Bioluminescent Zebrafish Screen for Enhancing Hematopoietic Cell Homing.

Stem cell reports

Astuti Y,Kramer AC,Blake AL,Blazar BR,Tolar J,Taisto ME,Lund TC

47117282 was used in flow cytometry to report that ergosterol increases hematopoietic cell homing in zebrafish and mice

Tue Jan 10 00:00:00 EST 2017

Mutant U2AF1-expressing cells are sensitive to pharmacological modulation of the spliceosome.

Nature communications

Shirai CL,White BS,Tripathi M,Tapia R,Ley JN,Ndonwi M,Kim S,Shao J,Carver A,Saez B,Fulton RS,Fronick C,O'Laughlin M,Lagisetti C,Webb TR,Graubert TA,Walter MJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 47-1172-82) in Flow Cytometry

Mon Jan 09 00:00:00 EST 2017

Core Circadian Clock Genes Regulate Leukemia Stem Cells in AML.

Cell

Puram RV,Kowalczyk MS,de Boer CG,Schneider RK,Miller PG,McConkey M,Tothova Z,Tejero H,Heckl D,Järås M,Chen MC,Li H,Tamayo A,Cowley GS,Rozenblatt-Rosen O,Al-Shahrour F,Regev A,Ebert BL

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 47-1172-82) in Flow Cytometry

Thu Apr 07 00:00:00 EDT 2016

Regulation of B-cell development and tolerance by different members of the miR-17∼92 family microRNAs.

Nature communications

Lai M,Gonzalez-Martin A,Cooper AB,Oda H,Jin HY,Shepherd J,He L,Zhu J,Nemazee D,Xiao C

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 47-1172-82) in Flow Cytometry

Tue Aug 02 00:00:00 EDT 2016

Identification of a CD133-CD55- population functions as a fetal common skeletal progenitor.

Scientific reports

Weng L,Hu X,Kumar B,Garcia M,Todorov I,Jung X,Marcucci G,Forman SJ,Chen CC

47-1172 was used in Flow cytometry/Cell sorting to identify a CD133-CD55- population in the fetal skeletal element that can generate bone and bone marrow.

Thu Dec 08 00:00:00 EST 2016

Geminin deletion increases the number of fetal hematopoietic stem cells by affecting the expression of key transcription factors.

Development (Cambridge, England)

Karamitros D,Patmanidi AL,Kotantaki P,Potocnik AJ,Bähr-Ivacevic T,Benes V,Lygerou Z,Kioussis D,Taraviras S

47-1172 was used in Flow cytometry/Cell sorting to investigate the role of geminin in hematopoiesis.

Thu Jan 01 00:00:00 EST 2015

Mast cells expedite control of pulmonary murine cytomegalovirus infection by enhancing the recruitment of protective CD8 T cells to the lungs.

PLoS pathogens

Ebert S,Becker M,Lemmermann NA,Büttner JK,Michel A,Taube C,Podlech J,Böhm V,Freitag K,Thomas D,Holtappels R,Reddehase MJ,Stassen M

47-1172 was used in Flow cytometry/Cell sorting to explore the role of mast cells in the recruitment of protective CD8 T cells to the lungs during systemic infection.

Tue Apr 01 00:00:00 EDT 2014

TRPA1-dependent pruritus in IL-13-induced chronic atopic dermatitis.

Journal of immunology (Baltimore, Md. : 1950)

Oh MH,Oh SY,Lu J,Lou H,Myers AC,Zhu Z,Zheng T

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 47-1172-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2013

Distribution of lentiviral vector integration sites in mice following therapeutic gene transfer to treat β-thalassemia.

Molecular therapy : the journal of the American Society of Gene Therapy

Ronen K,Negre O,Roth S,Colomb C,Malani N,Denaro M,Brady T,Fusil F,Gillet-Legrand B,Hehir K,Beuzard Y,Leboulch P,Down JD,Payen E,Bushman FD

47-1172 was used in Flow cytometry/Cell sorting to study integration sites of a lentiviral vector encoding β-globin during treatment of β-thalassemia, showing that integration occurred multiple times and clonal populations with integration near growth-control genes were not enriched.

Fri Jul 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: belly-spot; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit

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Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; Kit; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W

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UniProt ID: (Mouse) P05532

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Entrez Gene ID: (Mouse) 16590

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Function(s)

nucleotide binding protease binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity stem cell factor receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups cytokine binding protein homodimerization activity metal ion binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD117 (c-Kit) Monoclonal Antibody (ACK2), APC-eFluor™ 780, eBioscience™

CD117 (c-Kit) Antibody (47-1172-82) in Flow

Product Details

47-1172-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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