Invitrogen

CD11b Monoclonal Antibody (M1/70.15), PE-Texas Red

3 Published Figures

14 References

View all (223) CD11b antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (3)

FIGURE: 1 / 3

CD11b Antibody (RM2817) in Flow

Nature communications 2020 - Fig. 4 TMDI promotes enhanced inflammation and chemotaxis through the CXCL9/CXCL10-CXCR3 axis. a Percentages of adoptively transferred OTI recovered in different tissues 4 days post immunization with the depicted strategies ( n = 4, one representative of 2 independent experiments shown; one-way ANOVA with multiple comparisons; shown are means +- SD). b qPCR quantification of inflammatory cytokines in the la... View More

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Product Details

RM2817

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 13 publications 13 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

M1/70.15

Immunogen

C57BL/10 splenic T cells and concanavalin A stimulated C57BL/10 splenocytes

View immunogen

Conjugate

PE-Texas Red PE-Texas Red PE-Texas Red

Excitation/Emission Max

567/615 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

purified

Storage buffer

PBS with sucrose, 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373548

Product Specific Information

The M1/70.15 monoclonal antibody (mAb) reacts with the alpha-M subunit of Mac-1 (CD11b/CD18). For this PE-Texas Red™ conjugate: excitation 488 nm/peak emission 616 (nm). Applications for the M1/70.15 mAb include immunoprecipitation, immunostaining for flow cytometry, IHC of acetone-fixed frozen sections, and blocking of CR3-mediated rosetting.

Use dim light during handling, incubation with cells and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Every lot is tested by flow cytometry using freshly harvested mouse blood.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Low-dose radiation therapy suppresses viral pneumonia by enhancing broad-spectrum anti-inflammatory responses via transforming growth factor-β production.

Frontiers in immunology

Song HY,Chen F,Park HR,Han JM,Ji HJ,Byun EB,Kwon Y,Kim MK,Ahn KB,Seo HS

RM2817 was used in Flow cytometry/Cell sorting to find that LDRT-induced TGF-β reduced the levels of cytokines and polarized M2 cells and blocked immune cell infiltration, including neutrophils, in BALF.

Tue Jun 13 00:00:00 EDT 2023

The cephalic phase of insulin release is modulated by IL-1β.

Cell metabolism

Wiedemann SJ,Trimigliozzi K,Dror E,Meier DT,Molina-Tijeras JA,Rachid L,Le Foll C,Magnan C,Schulze F,Stawiski M,Häuselmann SP,Méreau H,Böni-Schnetzler M,Donath MY

RM2817 was used in Flow cytometry/Cell sorting to describe that this incretin-like effect of IL-1β involves neuronal transmission.

Tue Jul 05 00:00:00 EDT 2022

Therapeutic CFTR Correction Normalizes Systemic and Lung-Specific S1P Level Alterations Associated with Heart Failure.

International journal of molecular sciences

Uhl FE,Vanherle L,Matthes F,Meissner A

RM2817 was used in Flow cytometry/Cell sorting to suggest an important role for the CFTR-S1P axis in HF-mediated systemic and pulmonary inflammation.

Fri Jan 14 00:00:00 EST 2022

Impact of Antibiotic-Resistant Bacteria on Immune Activation and Clostridioides difficile Infection in the Mouse Intestine.

Infection and immunity

Keith JW,Dong Q,Sorbara MT,Becattini S,Sia JK,Gjonbalaj M,Seok R,Leiner IM,Littmann ER,Pamer EG

RM2817 was used in Flow cytometry/Cell sorting to test the hypothesis that the residual microbiota influences the severity of colitis caused by infection with Clostridioides difficile.

Mon Mar 23 00:00:00 EDT 2020

Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation.

Immunobiology

Jaiswal AK,Makhija S,Stahr N,Sandey M,Suryawanshi A,Mishra A

RM2817 was used in Flow Cytometry to support a model in which Alternaria sensitization in airways induce a circuitry of glycolysis and PKM2 regulation that confers an acute activation of APCs in the lung, whose targeting might represent a strategy for asthma treatment.

Wed Jul 01 00:00:00 EDT 2020

Manipulation of macrophage polarization by peptide-coated gold nanoparticles and its protective effects on acute lung injury.

Journal of nanobiotechnology

Wang L,Zhang H,Sun L,Gao W,Xiong Y,Ma A,Liu X,Shen L,Li Q,Yang H

RM2817 was used in Flow cytometry/Cell sorting to demonstrate that peptide-coated GNPs could induce M2 macrophage polarization in vitro and in vivo to effectively regulate lung inflammation, protect lung from injuries and promote inflammation resolution.

Wed Feb 26 00:00:00 EST 2020

Manipulation of macrophage polarization by peptide-coated gold nanoparticles and its protective effects on acute lung injury.

Journal of nanobiotechnology

Wang L,Zhang H,Sun L,Gao W,Xiong Y,Ma A,Liu X,Shen L,Li Q,Yang H

Wed Feb 26 00:00:00 EST 2020

Enhancing mucosal immunity by transient microbiota depletion.

Nature communications

Becattini S,Littmann ER,Seok R,Amoretti L,Fontana E,Wright R,Gjonbalaj M,Leiner IM,Plitas G,Hohl TM,Pamer EG

RM2817 was used in Flow cytometry/Cell sorting to investigate if transient microbiota depletion-boosted immunization is a useful model to dissect the requirements for optimal tissue resident memory CD8+ T cells responses in the intestine, and also a potential platform to devise novel mucosal vaccination approaches.

Tue Sep 08 00:00:00 EDT 2020

Enhancing mucosal immunity by transient microbiota depletion.

Nature communications

Becattini S,Littmann ER,Seok R,Amoretti L,Fontana E,Wright R,Gjonbalaj M,Leiner IM,Plitas G,Hohl TM,Pamer EG

Tue Sep 08 00:00:00 EDT 2020

Distinct Transcriptional Responses across Tissue-Resident Macrophages to Short-Term and Long-Term Metabolic Challenge.

Cell reports

Brykczynska U,Geigges M,Wiedemann SJ,Dror E,Böni-Schnetzler M,Hess C,Donath MY,Paro R

RM2817 was used in Flow cytometry/Cell sorting to provide a resource for assessing the impact of nutrition and over-nutrition on the spectrum of macrophages across tissues with a potential for identification of systemic responses.

Tue Feb 04 00:00:00 EST 2020

Therapeutic Delivery of miR-148a Suppresses Ventricular Dilation in Heart Failure.

Molecular therapy : the journal of the American Society of Gene Therapy

Raso A,Dirkx E,Philippen LE,Fernandez-Celis A,De Majo F,Sampaio-Pinto V,Sansonetti M,Juni R,El Azzouzi H,Calore M,Bitsch N,Olieslagers S,Oerlemans MIFJ,Huibers MM,de Weger RA,Reckman YJ,Pinto YM,Zentilin L,Zacchigna S,Giacca M,da Costa Martins PA,López-Andrés N,De Windt LJ

RM2817 was used in Immunocytochemistry-immunoflourescence to study the ability of miR-148a to prevent the transition of pressure-overload induced concentric hypertrophic remodelling toward eccentric hypertrophy and dilated cardiomyopathy.

Wed Mar 06 00:00:00 EST 2019

IL-6/Stat3-Dependent Induction of a Distinct, Obesity-Associated NK Cell Subpopulation Deteriorates Energy and Glucose Homeostasis.

Cell metabolism

Theurich S,Tsaousidou E,Hanssen R,Lempradl AM,Mauer J,Timper K,Schilbach K,Folz-Donahue K,Heilinger C,Sexl V,Pospisilik JA,Wunderlich FT,Brüning JC

RM2817 was used in Flow cytometry/Cell sorting to demonstrate that obesity-associated inflammation and metabolic disturbances depend on interleukin-6/Stat3-dependent formation of a distinct natural killer population, and provide a target for the treatment of obesity, metaflammation-associated pathologies, and diabetes.

Wed Jul 05 00:00:00 EDT 2017

Commensal microbes provide first line defense against Listeria monocytogenes infection.

The Journal of experimental medicine

Becattini S,Littmann ER,Carter RA,Kim SG,Morjaria SM,Ling L,Gyaltshen Y,Fontana E,Taur Y,Leiner IM,Pamer EG

RM2817 was used in Flow cytometry/Cell sorting to demonstrate a defensive role of the gut microbiota against Listeria monocytogenes infection.

Mon Jul 03 00:00:00 EDT 2017

IL-33-Dependent Group 2 Innate Lymphoid Cells Promote Cutaneous Wound Healing.

The Journal of investigative dermatology

Rak GD,Osborne LC,Siracusa MC,Kim BS,Wang K,Bayat A,Artis D,Volk SW

RM2817 was used in Flow cytometry/Cell sorting to demonstrate that cutaneous injury promotes an IL-33-dependent group 2 ILC response and that abrogation of this response can impair efficient wound closure.

Mon Feb 01 00:00:00 EST 2016

Kruppel-like Factor 6 Promotes Macrophage-mediated Inflammation by Suppressing B Cell Leukemia/Lymphoma 6 Expression.

The Journal of biological chemistry

Kim GD,Das R,Goduni L,McClellan S,Hazlett LD,Mahabeleshwar GH

RM2817 was used in flow cytometry to show that Kruppel-like transcription factor 6-B cell leukemia/lymphoma 6 regulates macrophage inflammatory gene expression and function

Fri Sep 30 00:00:00 EDT 2016

Allergic Lung Inflammation Reduces Tissue Invasion and Enhances Survival from Pulmonary Pneumococcal Infection in Mice, Which Correlates with Increased Expression of Transforming Growth Factor β1 and SiglecF(low) Alveolar Macrophages.

Infection and immunity

Sanfilippo AM,Furuya Y,Roberts S,Salmon SL,Metzger DW

RM2817 was used in Flow cytometry/Cell sorting to clarify the effects of asthma on subsequent susceptibility to pneumococcal infection.

Wed Jul 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD11 antigen-like family member B; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; integrin alpha-M; leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; MGC117044

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Gene Aliases: Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1

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Entrez Gene ID: (Mouse) 16409

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Function(s)

opsonin binding glycoprotein binding heparin binding heparan sulfate proteoglycan binding metal ion binding protein heterodimerization activity integrin

Process(es)

leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion integrin-mediated signaling pathway microglia development single organismal cell-cell adhesion neutrophil chemotaxis cellular extravasation activated T cell proliferation

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CD11b Monoclonal Antibody (M1/70.15), PE-Texas Red

CD11b Antibody (RM2817) in Flow

Product Details

RM2817

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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