Invitrogen

CD127 Monoclonal Antibody (A7R34), PE-Cyanine7, eBioscience™

FIGURE: 1 / 19

CD127 Antibody (25-1271-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 1.0 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 1.0 µg of Anti-Mouse CD127 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

25-1271-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 55 publications 55 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A7R34

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469649

Product Specific Information

Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.

Applications Reported: The A7R34 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

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Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD127 monoclonal antibody (Product # 25-1271-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

CD8 memory precursor cell generation is a continuous process.

iScience

Todorov H,Prieux M,Laubreton D,Bouvier M,Wang S,de Bernard S,Arpin C,Cannoodt R,Saelens W,Bonnaffoux A,Gandrillon O,Crauste F,Saeys Y,Marvel J

25-1271-82 was used in flow cytometry to study the generation of memory precursor cells following an acute infection by analyzing single-cell RNA-seq data that contained CD8 T cells collected during the postinfection expansion phase.

Fri Sep 16 00:00:00 EDT 2022

The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function.

eLife

Li S,Bern MD,Miao B,Fan C,Xing X,Inoue T,Piersma SJ,Wang T,Colonna M,Kurosaki T,Yokoyama WM

25-1271-82 was used in Flow cytometry/Cell sorting to investigate the role of Bach2 in natural killer (NK) cell development, revealing that Bach2 expression peaked in immature CD27+CD11b+ cells, and its deficiency led to increased proportions of terminally differentiated NK cells with enhanced granzyme and cytokine production, as well as improved NK cell-mediated control of tumor metastasis.

Mon Oct 03 00:00:00 EDT 2022

Tissue-specific transcriptional profiles and heterogeneity of natural killer cells and group 1 innate lymphoid cells.

Cell reports. Medicine

Lopes N,Galluso J,Escalière B,Carpentier S,Kerdiles YM,Vivier E

25-1271-82 was used in Flow cytometry/Cell sorting to reveal that the expressions of EOMES, GZMA, IRF8, JAK1, NKG7, PLEK, PRF1, and ZEB2 define NK cells and that IL7R, LTB, and RGS1 differentiate ILC1s from NK cells in mice and humans.

Tue Nov 15 00:00:00 EST 2022

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo.

Molecular medicine reports

Huo J,Liu T,Li F,Song X,Hou X

Published figure using CD127 monoclonal antibody (Product # 25-1271-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD127 monoclonal antibody (Product # 25-1271-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Hedgehog interacting protein-expressing lung fibroblasts suppress lymphocytic inflammation in mice.

JCI insight

Yun JH,Lee C,Liu T,Liu S,Kim EY,Xu S,Curtis JL,Pinello L,Bowler RP,Silverman EK,Hersh CP,Zhou X

Published figure using CD127 monoclonal antibody (Product # 25-1271-82) in Flow Cytometry

Wed Sep 08 00:00:00 EDT 2021

Single-Cell Analysis of Target Antigens of CAR-T Reveals a Potential Landscape of "On-Target, Off-Tumor Toxicity".

Frontiers in immunology

Zhang Y,Li Y,Cao W,Wang F,Xie X,Li Y,Wang X,Guo R,Jiang Z,Guo R

25-1271-82 was used in Flow Cytometry to identify the target antigen expression patterns of CAR therapies in 18 tissues and organs (peripheral blood mononuclear cells, bone marrow, lymph nodes, spleen, heart, ascending aortic tissue, trachea, lung, skin, kidney, bladder, esophagus, stomach, small intestine, rectum, liver, common bile duct, and pancreas) from healthy human samples.

Wed Feb 16 00:00:00 EST 2022

GM-CSF production by non-classical monocytes controls antagonistic LPS-driven functions in allergic inflammation.

Cell reports

Kaur K,Bachus H,Lewis C,Papillion AM,Rosenberg AF,Ballesteros-Tato A,León B

25-1271-82 was used in Flow cytometry/Cell sorting to show here that lipopolysaccharide activity switches from pro-pathogenic to protective depending on the production of granulocyte-macrophage colony-stimulating factor by non-classical monocytes.

Tue Dec 28 00:00:00 EST 2021

Heterogenous Populations of Tissue-Resident CD8⁺ T Cells Are Generated in Response to Infection and Malignancy.

Immunity

Milner JJ,Toma C,He Z,Kurd NS,Nguyen QP,McDonald B,Quezada L,Widjaja CE,Witherden DA,Crowl JT,Shaw LA,Yeo GW,Chang JT,Omilusik KD,Goldrath AW

25-1271 was used in Flow cytometry/Cell sorting to identify discrete lineages of intestinal antigen-specific CD8+ T cells, including a Blimp1hiId3lo tissue-resident effector cell population most prominent in the early phase of acute viral and bacterial infections and a molecularly distinct Blimp1loId3hi tissue-resident memory population that subsequently accumulated at later infection time points.

Tue May 19 00:00:00 EDT 2020

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using CD127 monoclonal antibody (Product # 25-1271-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

The cis-Regulatory Atlas of the Mouse Immune System.

Cell

Yoshida H,Lareau CA,Ramirez RN,Rose SA,Maier B,Wroblewska A,Desland F,Chudnovskiy A,Mortha A,Dominguez C,Tellier J,Kim E,Dwyer D,Shinton S,Nabekura T,Qi Y,Yu B,Robinette M,Kim KW,Wagers A,Rhoads A,Nutt SL,Brown BD,Mostafavi S,Buenrostro JD,Benoist C

25-1271-82 was used in Flow Cytometry to generate matched epigenome and transcriptome measurements in 86 primary cell types that span the mouse immune system and its differentiation cascade.

Thu Feb 07 00:00:00 EST 2019

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

Published figure using CD127 monoclonal antibody (Product # 25-1271-82) in Flow Cytometry

Tue Apr 16 00:00:00 EDT 2019

A Weaning Reaction to Microbiota Is Required for Resistance to Immunopathologies in the Adult.

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Al Nabhani Z,Dulauroy S,Marques R,Cousu C,Al Bounny S,Déjardin F,Sparwasser T,Bérard M,Cerf-Bensussan N,Eberl G

25-1271 was used in Flow cytometry/Cell sorting to explore the reactivity of the mouse intestinal immune system during the first weeks after birth and into adulthood.

Tue May 21 00:00:00 EDT 2019

A Mutation in the Transcription Factor Foxp3 Drives T Helper 2 Effector Function in Regulatory T Cells.

Immunity

Van Gool F,Nguyen MLT,Mumbach MR,Satpathy AT,Rosenthal WL,Giacometti S,Le DT,Liu W,Brusko TM,Anderson MS,Rudensky AY,Marson A,Chang HY,Bluestone JA

25-1271 was used in Flow cytometry/Cell sorting to identify an IPEX (immune dysregulation polyendocrinopathy enteropathy X-linked) syndrome patient with a FOXP3 mutation in the domain swap interface of the protein.

Tue Feb 19 00:00:00 EST 2019

Activation of naïve CD4⁺ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4⁺ T cells.

Nature immunology

Twohig JP,Cardus Figueras A,Andrews R,Wiede F,Cossins BC,Derrac Soria A,Lewis MJ,Townsend MJ,Millrine D,Li J,Hill DG,Uceda Fernandez J,Liu X,Szomolay B,Pepper CJ,Taylor PR,Pitzalis C,Tiganis T,Williams NM,Jones GW,Jones SA

Published figure using CD127 monoclonal antibody (Product # 25-1271-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

Bone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis.

Haematologica

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Sun Sep 01 00:00:00 EDT 2019

Single-Cell Profiling Defines Transcriptomic Signatures Specific to Tumor-Reactive versus Virus-Responsive CD4⁺ T Cells.

Cell reports

Magen A,Nie J,Ciucci T,Tamoutounour S,Zhao Y,Mehta M,Tran B,McGavern DB,Hannenhalli S,Bosselut R

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Tue Dec 03 00:00:00 EST 2019

Macrophage β2-Integrins Regulate IL-22 by ILC3s and Protect from Lethal Citrobacter rodentium-Induced Colitis.

Cell reports

Wang B,Lim JH,Kajikawa T,Li X,Vallance BA,Moutsopoulos NM,Chavakis T,Hajishengallis G

25-1271 was used in Flow cytometry/Cell sorting to conclude that β2-integrins are required for protective IL-1β-dependent IL-22 responses in colitis, and the identified mechanism may underlie the association of human LAD1 with colitis.

Tue Feb 05 00:00:00 EST 2019

Female and male mouse lung group 2 innate lymphoid cells differ in gene expression profiles and cytokine production.

PloS one

Mathä L,Shim H,Steer CA,Yin YH,Martinez-Gonzalez I,Takei F

25-1271-82 was used in Flow Cytometry to suggest that female ILC2s are more readily activated than male ILC2s due to their gene expression at the naïve state, which is potentially influenced by the lung environment.

Mon Dec 16 00:00:00 EST 2019

Aryl hydrocarbon receptor activation modulates γδ intestinal intraepithelial lymphocytes and protects against ischemia/reperfusion injury in the murine small intestine.

Molecular medicine reports

Zhang Z,Pu A,Yu M,Xiao W,Sun L,Cai Y,Yang H

25-1271-82 was used in Flow cytometry/Cell sorting to suggest that the AhR plays an important role in protecting the small intestine from I/R and increasing the γδIEL population by decreasing apoptosis of γδIELs.

Fri Mar 01 00:00:00 EST 2019

MicroRNA-150 controls differentiation of intraepithelial lymphocytes through TGF-β receptor II regulation.

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Seo SH,Jang MS,Kim DJ,Kim SM,Oh SC,Jung CR,Park Y,Ha SJ,Jung H,Park YJ,Yoon SR,Choi I,Kim TD

25-1271-82 was used in Flow Cytometry to investigate the regulation of intraepithelial lymphocyte differentiation and the underlying mechanisms in mice.

Sun Apr 01 00:00:00 EDT 2018

Attenuation of PKCδ enhances metabolic activity and promotes expansion of blood progenitors.

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Rao TN,Gupta MK,Softic S,Wang LD,Jang YC,Thomou T,Bezy O,Kulkarni RN,Kahn CR,Wagers AJ

25-1271-82 was used in Flow Cytometry to identify PKCδ as a critical regulator of HSPC signalling and metabolism that acts to limit HSPC expansion in response to physiological and regenerative demands.

Fri Dec 14 00:00:00 EST 2018

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

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International journal of molecular medicine

Luo B,Chen K,Feng Q,Xiao W,Ma D,Yang H,Zhang C

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Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Nature communications

Sun C,Lan P,Han Q,Huang M,Zhang Z,Xu G,Song J,Wang J,Wei H,Zhang J,Sun R,Zhang C,Tian Z

Published figure using CD127 monoclonal antibody (Product # 25-1271-82) in Flow Cytometry

Wed Mar 28 00:00:00 EDT 2018

Spred1 Safeguards Hematopoietic Homeostasis against Diet-Induced Systemic Stress.

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25-1271-82 was used in Flow Cytometry to demonstrate the involvement of diet-induced stress on hematopoietic stem cell self-renewal.

Thu May 03 00:00:00 EDT 2018

Characterization of the Inflammatory Response in Dystrophic Muscle Using Flow Cytometry.

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25-1271 was used in Flow cytometry/Cell sorting to highlight methods for the isolation and study of immune cell populations from muscles of mouse models of DMD.

Tue Jun 05 00:00:00 EDT 2018

Selective Expression of Flt3 within the Mouse Hematopoietic Stem Cell Compartment.

International journal of molecular sciences

Mooney CJ,Cunningham A,Tsapogas P,Toellner KM,Brown G

25-1271 was used in Flow cytometry/Cell sorting to investigate the functional heterogeneity of hematopoietic stem and progenitor cell populations.

Fri May 12 00:00:00 EDT 2017

Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells.

Cell

Yu VWC,Yusuf RZ,Oki T,Wu J,Saez B,Wang X,Cook C,Baryawno N,Ziller MJ,Lee E,Gu H,Meissner A,Lin CP,Kharchenko PV,Scadden DT

25127182 was used in flow cytometry to study epigenetic heterogeneity in hematopoietic stem cells.

Thu Nov 17 00:00:00 EST 2016

Hematopoietic Stem Cells Count and Remember Self-Renewal Divisions.

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Bernitz JM,Kim HS,MacArthur B,Sieburg H,Moore K

25-1271 was used in Flow cytometry/Cell sorting to suggest that HSCs accumulate discrete memory stages over their divisional history and identify cellular memory in HSC aging.

Thu Nov 17 00:00:00 EST 2016

Chronic interleukin-1 exposure drives haematopoietic stem cells towards precocious myeloid differentiation at the expense of self-renewal.

Nature cell biology

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25-1271 was used in Flow cytometry/Cell sorting to identify a critical regulatory circuit that tailors HSC responses to acute needs, and is likely to underlie deregulated blood homeostasis in chronic inflammation conditions.

Wed Jun 01 00:00:00 EDT 2016

Flt3 Ligand Regulates the Development of Innate Lymphoid Cells in Fetal and Adult Mice.

Journal of immunology (Baltimore, Md. : 1950)

Baerenwaldt A,von Burg N,Kreuzaler M,Sitte S,Horvath E,Peter A,Voehringer D,Rolink AG,Finke D

25-1271 was used in Flow cytometry/Cell sorting to investigate the role of the Flt3 ligand in the development and regulation of innate lymphoid cells in fetal and adult mice.

Tue Mar 15 00:00:00 EDT 2016

A simple, accurate and universal method for quantification of PCR.

BMC biotechnology

Boulter N,Suarez FG,Schibeci S,Sunderland T,Tolhurst O,Hunter T,Hodge G,Handelsman D,Simanainen U,Hendriks E,Duggan K

Published figure using CD127 monoclonal antibody (Product # 25-1271-82) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2016

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

25-1271 was used in Flow cytometry/Cell sorting to that Treg cell-derived IL-10 is needed to insulate, and promote the quality of, developing CD8(+) T cells.

Sat Aug 01 00:00:00 EDT 2015

Aging-associated inflammation promotes selection for adaptive oncogenic events in B cell progenitors.

The Journal of clinical investigation

Henry CJ,Casás-Selves M,Kim J,Zaberezhnyy V,Aghili L,Daniel AE,Jimenez L,Azam T,McNamee EN,Clambey ET,Klawitter J,Serkova NJ,Tan AC,Dinarello CA,DeGregori J

25-1271 was used in Flow cytometry/Cell sorting to conclude that chronic inflammatory microenvironments in old age lead to reductions in the fitness of B cell progenitor populations.

Tue Dec 01 00:00:00 EST 2015

High-level Gpr56 expression is dispensable for the maintenance and function of hematopoietic stem and progenitor cells in mice.

Stem cell research

Rao TN,Marks-Bluth J,Sullivan J,Gupta MK,Chandrakanthan V,Fitch SR,Ottersbach K,Jang YC,Piao X,Kulkarni RN,Serwold T,Pimanda JE,Wagers AJ

25-1271 was used in Flow cytometry/Cell sorting to indicate that high-level expression of GPR56 by primitive HSPCs is largely dispensable for steady-state and regenerative hematopoiesis.

Fri May 01 00:00:00 EDT 2015

The miR-126-VEGFR2 axis controls the innate response to pathogen-associated nucleic acids.

Nature immunology

Agudo J,Ruzo A,Tung N,Salmon H,Leboeuf M,Hashimoto D,Becker C,Garrett-Sinha LA,Baccarini A,Merad M,Brown BD

25-1271 was used in Flow cytometry/Cell sorting to identify the miR-126-VEGFR2 axis as an important regulator of the innate response that operates through multiscale control of pDCs.

Wed Jan 01 00:00:00 EST 2014

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

Published figure using CD127 monoclonal antibody (Product # 25-1271-82) in Flow Cytometry

Mon Sep 22 00:00:00 EDT 2014

High-frequency vaccine-induced CD8⁺ T cells specific for an epitope naturally processed during infection with Mycobacterium tuberculosis do not confer protection.

European journal of immunology

Lindenstrøm T,Aagaard C,Christensen D,Agger EM,Andersen P

25-1271 was used in Flow cytometry/Cell sorting to study the role of CD8 T cells in resistance to mycobacterial infections.

Sun Jun 01 00:00:00 EDT 2014

Innate lymphoid type 2 cells sustain visceral adipose tissue eosinophils and alternatively activated macrophages.

The Journal of experimental medicine

Molofsky AB,Nussbaum JC,Liang HE,Van Dyken SJ,Cheng LE,Mohapatra A,Chawla A,Locksley RM

25-1271 was used in Flow cytometry/Cell sorting to study how ILC2s are resident in visceral adipose tissue and promote eosinophils and alternatively activated macrophages, implicated in metabolic homeostasis.

Mon Mar 11 00:00:00 EDT 2013

Minor cell-death defects but reduced tumor latency in mice lacking the BH3-only proteins Bad and Bmf.

Oncogene

Baumgartner F,Woess C,Pedit V,Tzankov A,Labi V,Villunger A

25-1271 was used in Flow cytometry/Cell sorting to show that Bad and Bmf co-regulate lymphocyte homeostasis and limit spontaneous transformation in mice.

Thu Jan 31 00:00:00 EST 2013

Enhancing T lineage production in aged mice: a novel function of Foxn1 in the bone marrow niche.

Journal of immunology (Baltimore, Md. : 1950)

Zook EC,Zhang S,Gerstein RM,Witte PL,Le PT

25-1271 was used in Flow cytometry/Cell sorting to report that Foxn1 is expressed in wild-type BM and overexpressed in Foxn1Tg.

Sun Dec 01 00:00:00 EST 2013

IL-33 signaling regulates innate and adaptive immunity to Cryptococcus neoformans.

Journal of immunology (Baltimore, Md. : 1950)

Flaczyk A,Duerr CU,Shourian M,Lafferty EI,Fritz JH,Qureshi ST

25-1271 was used in Flow cytometry/Cell sorting to study how allergic patterns of lung inflammation are mediated as a host response to Cryptococcus neoformans infection.

Sun Sep 01 00:00:00 EDT 2013

RAG-1 and Ly6D independently reflect progression in the B lymphoid lineage.

PloS one

Zhang Q,Esplin BL,Iida R,Garrett KP,Huang ZL,Medina KL,Kincade PW

25-1271 was used in Flow cytometry/Cell sorting to elucidate the mechanisms regulating the differentiation path from haematopoietic stem cells to B lineage lymphocytes.

Tue Apr 15 00:00:00 EDT 2014

A self-reinforcing regulatory network triggered by limiting IL-7 activates pre-BCR signaling and differentiation.

Nature immunology

Ochiai K,Maienschein-Cline M,Mandal M,Triggs JR,Bertolino E,Sciammas R,Dinner AR,Clark MR,Singh H

Sun Jan 22 00:00:00 EST 2012

Protein energy malnutrition impairs homeostatic proliferation of memory CD8 T cells.

Journal of immunology (Baltimore, Md. : 1950)

Iyer SS,Chatraw JH,Tan WG,Wherry EJ,Becker TC,Ahmed R,Kapasi ZF

25-1271 was used in Flow cytometry/Cell sorting to show a metabolic requirement of dietary protein in sustaining functional CD8 memory.

Sun Jan 01 00:00:00 EST 2012

The transcriptional regulators Id2 and Id3 control the formation of distinct memory CD8+ T cell subsets.

Nature immunology

Yang CY,Best JA,Knell J,Yang E,Sheridan AD,Jesionek AK,Li HS,Rivera RR,Lind KC,D'Cruz LM,Watowich SS,Murre C,Goldrath AW

25-1271 was used in Flow cytometry/Cell sorting to elucidate the mechanisms of regulation of terminal differentiation of CD8(+) T cells.

Sun Nov 06 00:00:00 EDT 2011

A peripheral CD4+ T cell precursor for naive, memory, and regulatory T cells.

The Journal of experimental medicine

Zhao C,Davies JD

25-1271 was used in Flow cytometry/Cell sorting to support a key role for CD4(+) CD44(v.low) cells as peripheral precursors that maintain the integrity of the CD4(+) T cell pool.

Mon Dec 20 00:00:00 EST 2010

Route of adenovirus-based HIV-1 vaccine delivery impacts the phenotype and trafficking of vaccine-elicited CD8+ T lymphocytes.

Journal of virology

Kaufman DR,Bivas-Benita M,Simmons NL,Miller D,Barouch DH

25-1271 was used in Flow cytometry/Cell sorting to indicate that the anatomic microenvironment of antigen expression critically impacts the phenotype and trafficking of antigen-specific CD8(+) T lymphocytes.

Tue Jun 01 00:00:00 EDT 2010

Efficient generation of mucosal and systemic antigen-specific CD8+ T-cell responses following pulmonary DNA immunization.

Journal of virology

Bivas-Benita M,Bar L,Gillard GO,Kaufman DR,Simmons NL,Hovav AH,Letvin NL

25-1271 was used in Flow cytometry/Cell sorting to evaluate the ability of plasmid DNA to induce antigen-specific immune cell responses after pulmonary administration.

Tue Jun 01 00:00:00 EDT 2010

Langerhans cell (LC) proliferation mediates neonatal development, homeostasis, and inflammation-associated expansion of the epidermal LC network.

The Journal of experimental medicine

Chorro L,Sarde A,Li M,Woollard KJ,Chambon P,Malissen B,Kissenpfennig A,Barbaroux JB,Groves R,Geissmann F

25-1271 was used in Flow cytometry/Cell sorting to investigate the mechanisms involved in Langerhans cell development and homeostasis.

Mon Dec 21 00:00:00 EST 2009

Infection with a helminth parasite attenuates autoimmunity through TGF-beta-mediated suppression of Th17 and Th1 responses.

Journal of immunology (Baltimore, Md. : 1950)

Walsh KP,Brady MT,Finlay CM,Boon L,Mills KH

25-1271 was used in Flow cytometry/Cell sorting to provide evidence that parasites can exert bystander immunosuppression of pathogenic T cells that mediate autoimmune diseases.

Sat Aug 01 00:00:00 EDT 2009

LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.

Journal of immunology (Baltimore, Md. : 1950)

Vondenhoff MF,Greuter M,Goverse G,Elewaut D,Dewint P,Ware CF,Hoorweg K,Kraal G,Mebius RE

Fri May 01 00:00:00 EDT 2009

Trafficking of antigen-specific CD8+ T lymphocytes to mucosal surfaces following intramuscular vaccination.

Journal of immunology (Baltimore, Md. : 1950)

Kaufman DR,Liu J,Carville A,Mansfield KG,Havenga MJ,Goudsmit J,Barouch DH

25-1271 was used in Flow cytometry/Cell sorting to evaluate mucosal cellular immune responses in mice and rhesus monkeys after systemic immunisation with various replication-incompetent recombinant adenovirus vectors.

Mon Sep 15 00:00:00 EDT 2008

Rapid memory CD8+ T-lymphocyte induction through priming with recombinant Mycobacterium smegmatis.

Journal of virology

Hovav AH,Cayabyab MJ,Panas MW,Santra S,Greenland J,Geiben R,Haynes BF,Jacobs WR,Letvin NL

25-1271 was used in Flow cytometry/Cell sorting to investigate novel priming immunogens that might be used in heterologous immunization regimens, showing that priming with Mycobacterium smegmatis leads to rapid memory CD8+ T-lymphocyte induction.

Mon Jan 01 00:00:00 EST 2007

Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis.

The American journal of pathology

Leithäuser F,Meinhardt-Krajina T,Fink K,Wotschke B,Möller P,Reimann J

Thu Jun 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: CD127; IL 7 receptor; IL 7R a; IL 7R subunit alpha; IL 7R α; IL 7Ra; IL 7Rα; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; IL7 receptor; IL7 receptor subunit alpha; IL7R a; IL7R alpha; IL7R subunit alpha; IL7R α; IL7Rα; interleukin 7 receptor alpha chain; Interleukin-7 receptor subunit alpha; Interleukin7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7R

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Gene Aliases: CD127; IL-7Ralpha; Il7r

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UniProt ID: (Mouse) P16872

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Entrez Gene ID: (Mouse) 16197

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Function(s)

cytokine receptor activity transmembrane signal receptor

Process(es)

cell morphogenesis negative regulation of T cell mediated cytotoxicity immunoglobulin production regulation of cell size positive regulation of gene expression cell growth T cell differentiation positive regulation of T cell differentiation in thymus B cell proliferation lymph node development homeostasis of number of cells

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CD127 Monoclonal Antibody (A7R34), PE-Cyanine7, eBioscience™

CD127 Antibody (25-1271-82) in Flow

Product Details

25-1271-82

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

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Published species

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Recommended Isotype Control

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