Invitrogen

CD127 Monoclonal Antibody (A7R34), PerCP-Cyanine5.5, eBioscience™

FIGURE: 1 / 19

CD127 Antibody (45-1271-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.5 µg of Rat IgG2a K Isotype Control PerCP-Cyanine5-5 (Product # 45-4321-80) (left) or 0.125 µg of Anti-Mouse CD127 PerCP-Cyanine5-5 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

45-1271-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 33 publications 33 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A7R34

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1106998

Product Specific Information

Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.

Applications Reported: This A7R34 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo.

Molecular medicine reports

Huo J,Liu T,Li F,Song X,Hou X

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Hedgehog interacting protein-expressing lung fibroblasts suppress lymphocytic inflammation in mice.

JCI insight

Yun JH,Lee C,Liu T,Liu S,Kim EY,Xu S,Curtis JL,Pinello L,Bowler RP,Silverman EK,Hersh CP,Zhou X

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Wed Sep 08 00:00:00 EDT 2021

SRC-3 Functions as a Coactivator of T-bet by Regulating the Maturation and Antitumor Activity of Natural Killer Cells.

Cancer immunology research

Hu M,Lu Y,Qi Y,Zhang Z,Wang S,Xu Y,Chen F,Tang Y,Chen S,Chen M,Du C,Shen M,Wang F,Su Y,Deng Y,Wang J

45-1271-82 was used in Flow Cytometry to study the role of SRC-3 as a coactivator of T-bet in natural killer-cell biology and indicate that targeting SRC-3 may be a promising strategy to increase the tumor surveillance function of natural killer cells.

Tue Sep 01 00:00:00 EDT 2020

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Tue Apr 16 00:00:00 EDT 2019

Activation of naïve CD4⁺ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4⁺ T cells.

Nature immunology

Twohig JP,Cardus Figueras A,Andrews R,Wiede F,Cossins BC,Derrac Soria A,Lewis MJ,Townsend MJ,Millrine D,Li J,Hill DG,Uceda Fernandez J,Liu X,Szomolay B,Pepper CJ,Taylor PR,Pitzalis C,Tiganis T,Williams NM,Jones GW,Jones SA

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

Bone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis.

Haematologica

Zhang Z,Huang Z,Ong B,Sahu C,Zeng H,Ruan HB

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Single-Cell Profiling Defines Transcriptomic Signatures Specific to Tumor-Reactive versus Virus-Responsive CD4⁺ T Cells.

Cell reports

Magen A,Nie J,Ciucci T,Tamoutounour S,Zhao Y,Mehta M,Tran B,McGavern DB,Hannenhalli S,Bosselut R

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Tue Dec 03 00:00:00 EST 2019

Phc2 controls hematopoietic stem and progenitor cell mobilization from bone marrow by repressing Vcam1 expression.

Nature communications

Bae J,Choi SP,Isono K,Lee JY,Park SW,Choi CY,Han J,Kim SH,Lee HH,Park K,Jin HY,Lee SJ,Park CG,Koseki H,Lee YS,Chun T

45-1271-82 was used in Flow cytometry/Cell sorting to determine the role of PHc2 in controlling the mobilisation of hematopoietic stem and progenitor cells.

Fri Aug 02 00:00:00 EDT 2019

Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.

Nature medicine

Maryanovich M,Zahalka AH,Pierce H,Pinho S,Nakahara F,Asada N,Wei Q,Wang X,Ciero P,Xu J,Leftin A,Frenette PS

45-1271 was used in Flow cytometry/Cell sorting to determine whether haematopoietic stem cell ageing depends on bone marrow innervation by the sympathetic nervous system.

Fri Jun 01 00:00:00 EDT 2018

Dependence of innate lymphoid cell 1 development on NKp46.

PLoS biology

Wang Y,Dong W,Zhang Y,Caligiuri MA,Yu J

45-1271 was used in Flow cytometry/Cell sorting to reveal that NKp46 selectively participates in the regulation of ILC1 development.

Sun Apr 01 00:00:00 EDT 2018

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

The interplay of BMP4 and IL‑7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia̸reperfusion.

International journal of molecular medicine

Luo B,Chen K,Feng Q,Xiao W,Ma D,Yang H,Zhang C

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Nature communications

Sun C,Lan P,Han Q,Huang M,Zhang Z,Xu G,Song J,Wang J,Wei H,Zhang J,Sun R,Zhang C,Tian Z

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Wed Mar 28 00:00:00 EDT 2018

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

45-1271 was used in Flow cytometry/Cell sorting to identify components of the transforming growth factor Β pathway as key targets of miR-143/145.

Wed Jun 20 00:00:00 EDT 2018

MERTK inhibition alters the PD-1 axis and promotes anti-leukemia immunity.

JCI insight

Lee-Sherick AB,Jacobsen KM,Henry CJ,Huey MG,Parker RE,Page LS,Hill AA,Wang X,Frye SV,Earp HS,Jordan CT,DeRyckere D,Graham DK

45-1271 was used in Flow cytometry/Cell sorting to demonstrate both the direct therapeutic effects of MERTK inhibition on leukaemia cells and the induction of anti-leukaemia immunity via suppression of the coinhibitory PD-1 axis.

Fri Nov 02 00:00:00 EDT 2018

Stem cell factor is selectively secreted by arterial endothelial cells in bone marrow.

Nature communications

Xu C,Gao X,Wei Q,Nakahara F,Zimmerman SE,Mar J,Frenette PS

45-1271 was used in Flow cytometry/Cell sorting to characterise the expression signature that distinguishes arterial endothelial cells from sinusoidal endothelial cells.

Fri Jun 22 00:00:00 EDT 2018

lncRNA requirements for mouse acute myeloid leukemia and normal differentiation.

eLife

Delás MJ,Sabin LR,Dolzhenko E,Knott SR,Munera Maravilla E,Jackson BT,Wild SA,Kovacevic T,Stork EM,Zhou M,Erard N,Lee E,Kelley DR,Roth M,Barbosa IA,Zuber J,Rinn JL,Smith AD,Hannon GJ

45-1271 was used in Flow cytometry/Cell sorting to assess lncRNA function via an in vivo RNAi screen in a model of acute myeloid leukemia.

Wed Sep 06 00:00:00 EDT 2017

A simple, accurate and universal method for quantification of PCR.

BMC biotechnology

Boulter N,Suarez FG,Schibeci S,Sunderland T,Tolhurst O,Hunter T,Hodge G,Handelsman D,Simanainen U,Hendriks E,Duggan K

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2016

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

CD70 and IFN-1 selectively induce eomesodermin or T-bet and synergize to promote CD8+ T-cell responses.

European journal of immunology

Dong H,Franklin NA,Ritchea SB,Yagita H,Glennie MJ,Bullock TN

45-1271 was used in Flow cytometry/Cell sorting to identify complex interactions between signal 3 and costimulatory pathways, and identify opportunities to influence the differentiation of CD8(+) T-cell responses.

Tue Dec 01 00:00:00 EST 2015

IL-7/IL-7 Receptor Signaling Differentially Affects Effector CD4+ T Cell Subsets Involved in Experimental Autoimmune Encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Arbelaez CA,Glatigny S,Duhen R,Eberl G,Oukka M,Bettelli E

45-1271 was used in Flow cytometry/Cell sorting to address the contribution of IL-23/IL-23R and IL-7/IL-7R signaling in Th17 and Th1 cell dynamics during CNS autoimmunity.

Tue Sep 01 00:00:00 EDT 2015

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Mon Sep 22 00:00:00 EDT 2014

Chromosome instability underlies hematopoietic stem cell dysfunction and lymphoid neoplasia associated with impaired Fbw7-mediated cyclin E regulation.

Molecular and cellular biology

Siu KT,Xu Y,Swartz KL,Bhattacharyya M,Gurbuxani S,Hua Y,Minella AC

45-1271 was used in Flow cytometry/Cell sorting to investigate the contribution of individual substrate ubiquitination pathways to hematopoietic stem cell homeostasis, showing that impaired Fbw7-mediated cyclin E regulation leads to chromosome instability.

Mon Sep 01 00:00:00 EDT 2014

Type 2 innate lymphoid cells control eosinophil homeostasis.

Nature

Nussbaum JC,Van Dyken SJ,von Moltke J,Cheng LE,Mohapatra A,Molofsky AB,Thornton EE,Krummel MF,Chawla A,Liang HE,Locksley RM

45-1271 was used in Flow cytometry/Cell sorting to investigate the processes underlying regulation of cytokine and chemokines for eosinophil development and survival, showing that type 2 innate lymphoid cells control eosinophil homeostasis.

Thu Oct 10 00:00:00 EDT 2013

Innate lymphoid type 2 cells sustain visceral adipose tissue eosinophils and alternatively activated macrophages.

The Journal of experimental medicine

Molofsky AB,Nussbaum JC,Liang HE,Van Dyken SJ,Cheng LE,Mohapatra A,Chawla A,Locksley RM

45-1271 was used in Flow cytometry/Cell sorting to study how ILC2s are resident in visceral adipose tissue and promote eosinophils and alternatively activated macrophages, implicated in metabolic homeostasis.

Mon Mar 11 00:00:00 EDT 2013

Deletion of the vaccinia virus gene A46R, encoding for an inhibitor of TLR signalling, is an effective approach to enhance the immunogenicity in mice of the HIV/AIDS vaccine candidate NYVAC-C.

PloS one

Perdiguero B,Gómez CE,Di Pilato M,Sorzano CO,Delaloye J,Roger T,Calandra T,Pantaleo G,Esteban M

45-1271 was used in Flow cytometry/Cell sorting to establish the immunological role of VACV A46R on innate immune responses of macrophages in vitro and antigen-specific T and B cell immune responses in vivo.

Sun Jun 15 00:00:00 EDT 2014

Early infection with respiratory syncytial virus impairs regulatory T cell function and increases susceptibility to allergic asthma.

Nature medicine

Krishnamoorthy N,Khare A,Oriss TB,Raundhal M,Morse C,Yarlagadda M,Wenzel SE,Moore ML,Peebles RS,Ray A,Ray P

45-1271 was used in Flow cytometry/Cell sorting to highlight a mechanism by which viral infection targets a host-protective mechanism in early life and increases susceptibility to allergic disease.

Mon Oct 01 00:00:00 EDT 2012

A self-reinforcing regulatory network triggered by limiting IL-7 activates pre-BCR signaling and differentiation.

Nature immunology

Ochiai K,Maienschein-Cline M,Mandal M,Triggs JR,Bertolino E,Sciammas R,Dinner AR,Clark MR,Singh H

Published figure using CD127 monoclonal antibody (Product # 45-1271-82) in Flow Cytometry

Sun Jan 22 00:00:00 EST 2012

Chronic IFN-γ production in mice induces anemia by reducing erythrocyte life span and inhibiting erythropoiesis through an IRF-1/PU.1 axis.

Blood

Libregts SF,Gutiérrez L,de Bruin AM,Wensveen FM,Papadopoulos P,van Ijcken W,Ozgür Z,Philipsen S,Nolte MA

45-1271 was used in Flow cytometry/Cell sorting to reveal a molecular mechanism by which chronic exposure to IFN-γ induces expression of IRF-1 and ultimately induces anaemia.

Thu Sep 01 00:00:00 EDT 2011

Infection-induced myelopoiesis during intracellular bacterial infection is critically dependent upon IFN-γ signaling.

Journal of immunology (Baltimore, Md. : 1950)

MacNamara KC,Oduro K,Martin O,Jones DD,McLaughlin M,Choi K,Borjesson DL,Winslow GM

45-1271 was used in Flow cytometry/Cell sorting to provide evidence that IFN-γ acts to control infection by directly promoting myeloid cell differentiation.

Sat Jan 15 00:00:00 EST 2011

LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.

Journal of immunology (Baltimore, Md. : 1950)

Vondenhoff MF,Greuter M,Goverse G,Elewaut D,Dewint P,Ware CF,Hoorweg K,Kraal G,Mebius RE

Fri May 01 00:00:00 EDT 2009

Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis.

The American journal of pathology

Leithäuser F,Meinhardt-Krajina T,Fink K,Wotschke B,Möller P,Reimann J

Thu Jun 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: CD127; IL 7 receptor; IL 7R a; IL 7R subunit alpha; IL 7R α; IL 7Ra; IL 7Rα; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; IL7 receptor; IL7 receptor subunit alpha; IL7R a; IL7R alpha; IL7R subunit alpha; IL7R α; IL7Rα; interleukin 7 receptor alpha chain; Interleukin-7 receptor subunit alpha; Interleukin7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7R

View more View less

Gene Aliases: CD127; IL-7Ralpha; Il7r

View more View less

UniProt ID: (Mouse) P16872

View more View less

Entrez Gene ID: (Mouse) 16197

View more View less

Function(s)

cytokine receptor activity transmembrane signal receptor

Process(es)

cell morphogenesis negative regulation of T cell mediated cytotoxicity immunoglobulin production regulation of cell size positive regulation of gene expression cell growth T cell differentiation positive regulation of T cell differentiation in thymus B cell proliferation lymph node development homeostasis of number of cells

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD127 Monoclonal Antibody (A7R34), PerCP-Cyanine5.5, eBioscience™

CD127 Antibody (45-1271-82) in Flow

Product Details

45-1271-82

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!