Invitrogen

CD135 (Flt3) Monoclonal Antibody (A2F10), PE-Cyanine5, eBioscience™

CD135 (Flt3) Antibody in Flow Cytometry (Flow)

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CD135 (Flt3) Antibody (15-1351-82) in Flow

Staining of BALB/c bone marrow cells with Anti-Mouse CD45-1 FITC (Product # 11-0453-82) and 0.5 µg of Anti-Mouse CD135 (Flt3) PE-Cyanine5.Total viable cells were used for analysis.

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Product Details

15-1351-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 26 publications 26 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A2F10

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_494219

Product Specific Information

Description: The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.

Applications Reported: This A2F10 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression.

International journal of molecular sciences

Li N,Wang Y,Wang A,Zhang J,Jia C,Yu C,Song Z,Wang S,Liu L,Yi J,Bao Y,Huang Y,Sun L

Published figure using CD135 (Flt3) monoclonal antibody (Product # 15-1351-82) in Flow Cytometry

Fri Feb 03 00:00:00 EST 2023

Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice.

STAR protocols

Liu C,Lin X,Jin Y,Pan J

15-1351-82 was used in flow cytometry to describe practical procedures for sorting and analysis of leukemia stem cells (LSCs) to improve our understanding of chronic myelogenous leukemia (CML).

Fri Mar 17 00:00:00 EDT 2023

Erythropoietin directly remodels the clonal composition of murine hematopoietic multipotent progenitor cells.

eLife

Eisele AS,Cosgrove J,Magniez A,Tubeuf E,Tenreira Bento S,Conrad C,Cayrac F,Tak T,Lyne AM,Urbanus J,Perié L

15-1351-82 was used in flow cytometry to show that EPO does act directly on MPP independent of the niche and modulates fate by remodeling the clonal composition of the MPP pool.

Tue Feb 15 00:00:00 EST 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD135 (Flt3) monoclonal antibody (Product # 15-1351-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

TNF-α-induced alterations in stromal progenitors enhance leukemic stem cell growth via CXCR2 signaling.

Cell reports

Agarwal P,Li H,Choi K,Hueneman K,He J,Welner RS,Starczynowski DT,Bhatia R

15-1351-82 was used in Flow Cytometry to find that TNF-α-mediated alterations in CML BM stromal niches enhance support of LSC maintenance and growth via CXCL1-CXCR2 signaling and that CXCR2 inhibition effectively depletes CML LSCs.

Tue Jul 13 00:00:00 EDT 2021

Mesenchymal stem cells attenuate liver fibrosis by targeting Ly6C^hi/lo macrophages through activating the cytokine-paracrine and apoptotic pathways.

Cell death discovery

Li YH,Shen S,Shao T,Jin MT,Fan DD,Lin AF,Xiang LX,Shao JZ

15-1351-82 was used in Flow Cytometry to provide insights into the functional role of bone marrow-derived sesenchymal stem cell in alleviating liver fibrosis.

Mon Sep 13 00:00:00 EDT 2021

Hydrogels Containing Gradients in Vascular Density Reveal Dose-Dependent Role of Angiocrine Cues on Stem Cell Behavior.

Advanced functional materials

Ngo MT,Barnhouse VR,Gilchrist AE,Mahadik BP,Hunter CJ,Hensold JN,Petrikas N,Harley BAC

15-1351-82 was used in Flow cytometry/Cell sorting to report the successful development of a microfluidic device to generate hydrogels containing lateral gradients in vascular density as a means to identify angiocrine signals underlying hematopoietic stem cell culture ex vivo.

Thu Dec 16 00:00:00 EST 2021

Adaptive response to inflammation contributes to sustained myelopoiesis and confers a competitive advantage in myelodysplastic syndrome HSCs.

Nature immunology

Muto T,Walker CS,Choi K,Hueneman K,Smith MA,Gul Z,Garcia-Manero G,Ma A,Zheng Y,Starczynowski DT

15-1351 was used in Flow cytometry/Cell sorting to show that chronic inflammation is a determinant for the competitive advantage of myelodysplastic syndrome (MDS) hematopoietic stem and progenitor cells (HSPCs) and for disease progression.

Fri May 01 00:00:00 EDT 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD135 (Flt3) monoclonal antibody (Product # 15-1351-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Zinc finger and BTB domain-containing protein 46 is essential for survival and proliferation of acute myeloid leukemia cell line but dispensable for normal hematopoiesis.

Chinese medical journal

Liu YY,Xiao FF,Yang BJ,Li X,Xu SN,Chen ZW,Li P,Huang YX,Fu XM,Huang XQ,Zheng GL,Chen JP,Hou Y

Published figure using CD135 (Flt3) monoclonal antibody (Product # 15-1351-82) in Flow Cytometry

Mon Jul 20 00:00:00 EDT 2020

Connecting secretome to hematopoietic stem cell phenotype shifts in an engineered bone marrow niche.

Integrative biology : quantitative biosciences from nano to macro

Gilchrist AE,Harley BAC

15-1351-82 was used in Flow Cytometry to investigate the mechanism of how marrow-derived mesenchymal stromal and progenitor cells impacts hematopoietic stem cells fate decisions.

Fri Jul 10 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD135 (Flt3) monoclonal antibody (Product # 15-1351-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

Published figure using CD135 (Flt3) monoclonal antibody (Product # 15-1351-82) in Flow Cytometry

Tue Apr 16 00:00:00 EDT 2019

Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow.

Immunity

Walker JA,Clark PA,Crisp A,Barlow JL,Szeto A,Ferreira ACF,Rana BMJ,Jolin HE,Rodriguez-Rodriguez N,Sivasubramaniam M,Pannell R,Cruickshank J,Daly M,Haim-Vilmovsky L,Teichmann SA,McKenzie ANJ

Published figure using CD135 (Flt3) monoclonal antibody (Product # 15-1351-82) in Flow Cytometry

Tue Jul 16 00:00:00 EDT 2019

Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells.

Cell stem cell

Agarwal P,Isringhausen S,Li H,Paterson AJ,He J,Gomariz Á,Nagasawa T,Nombela-Arrieta C,Bhatia R

15-1351-82 was used in Flow Cytometry to highlight a critical role of niche-specific effects of Cxcl12 expression in maintaining quiescence of TKI-resistant LSC populations.

Thu May 02 00:00:00 EDT 2019

MicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity.

Frontiers in immunology

Knolle MD,Chin SB,Rana BMJ,Englezakis A,Nakagawa R,Fallon PG,Git A,McKenzie ANJ

15-1351 was used in Flow cytometry/Cell sorting to identify a subset of micro-RNAs that are regulated upon group-2 innate lymphoid cells (ILC2) activation, and establish a specific role for miR-155 in regulating ILC2 survival following activation.

Mon Sep 30 00:00:00 EDT 2019

Ubiquitination of hnRNPA1 by TRAF6 links chronic innate immune signaling with myelodysplasia.

Nature immunology

Fang J,Bolanos LC,Choi K,Liu X,Christie S,Akunuru S,Kumar R,Wang D,Chen X,Greis KD,Stoilov P,Filippi MD,Maciejewski JP,Garcia-Manero G,Weirauch MT,Salomonis N,Geiger H,Zheng Y,Starczynowski DT

15-1351 was used in Flow cytometry/Cell sorting to examine the significance of ubiquitin signalling in coordinating RNA processing by Toll-like receptor pathways during an immune response and in premalignant haematologic diseases.

Wed Feb 01 00:00:00 EST 2017

LCN2 overexpression in bone enhances the hematopoietic compartment via modulation of the bone marrow microenvironment.

Journal of cellular physiology

Costa D,Principi E,Lazzarini E,Descalzi F,Cancedda R,Castagnola P,Tavella S

15135182 was used in flow cytometry to examine the function of lipocalin-2 in bone tissue

Wed Nov 01 00:00:00 EDT 2017

A single-cell resolution map of mouse hematopoietic stem and progenitor cell differentiation.

Blood

Nestorowa S,Hamey FK,Pijuan Sala B,Diamanti E,Shepherd M,Laurenti E,Wilson NK,Kent DG,Göttgens B

15-1351 was used in Flow cytometry/Cell sorting to report the development of an intuitive Web interface as a new community resource to permit visualisation of gene expression in haematopoietic stem and progenitor cells at single-cell resolution for any gene of choice.

Thu Aug 25 00:00:00 EDT 2016

Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton.

eLife

Yue R,Shen B,Morrison SJ

15-1351 was used in Flow cytometry/Cell sorting to study how Clec11a/Osteolectin maintains the adult skeleton by promoting the differentiation of mesenchymal progenitors into mature osteoblasts.

Tue Dec 13 00:00:00 EST 2016

Index sorting resolves heterogeneous murine hematopoietic stem cell populations.

Experimental hematology

Schulte R,Wilson NK,Prick JC,Cossetti C,Maj MK,Gottgens B,Kent DG

15-1351 was used in Flow cytometry/Cell sorting to indicate that when used in combination with single-cell functional assays, index sorting is a powerful tool for refining cell isolation strategies.

Tue Sep 01 00:00:00 EDT 2015

Oestrogen increases haematopoietic stem-cell self-renewal in females and during pregnancy.

Nature

Nakada D,Oguro H,Levi BP,Ryan N,Kitano A,Saitoh Y,Takeichi M,Wendt GR,Morrison SJ

15-1351 was used in Flow cytometry/Cell sorting to assess the effect of oestradiol administration on division of haematopoietic stem cells in males and females.

Thu Jan 23 00:00:00 EST 2014

HIF prolyl hydroxylase 2 (PHD2) is a critical regulator of hematopoietic stem cell maintenance during steady-state and stress.

Blood

Singh RP,Franke K,Kalucka J,Mamlouk S,Muschter A,Gembarska A,Grinenko T,Willam C,Naumann R,Anastassiadis K,Stewart AF,Bornstein S,Chavakis T,Breier G,Waskow C,Wielockx B

15-1351 was used in Flow cytometry/Cell sorting to study the role of PHD2 in HSC compartment maintenance.

Thu Jun 27 00:00:00 EDT 2013

An information theoretic, microfluidic-based single cell analysis permits identification of subpopulations among putatively homogeneous stem cells.

PloS one

Glotzbach JP,Januszyk M,Vial IN,Wong VW,Gelbard A,Kalisky T,Thangarajah H,Longaker MT,Quake SR,Chu G,Gurtner GC

15-1351 was used in Flow cytometry/Cell sorting to develop a novel method to characterize and predict transcriptional programs across individual homogeneous stem cells.

Wed Nov 02 00:00:00 EDT 2011

CX3CR1+ CD115+ CD135+ common macrophage/DC precursors and the role of CX3CR1 in their response to inflammation.

The Journal of experimental medicine

Auffray C,Fogg DK,Narni-Mancinelli E,Senechal B,Trouillet C,Saederup N,Leemput J,Bigot K,Campisi L,Abitbol M,Molina T,Charo I,Hume DA,Cumano A,Lauvau G,Geissmann F

15-1351 was used in Flow cytometry/Cell sorting to show that macrophage/DC precursors give rise to conventional DCs,.

Mon Mar 16 00:00:00 EDT 2009

Niche recycling through division-independent egress of hematopoietic stem cells.

The Journal of experimental medicine

Bhattacharya D,Czechowicz A,Ooi AG,Rossi DJ,Bryder D,Weissman IL

15-1351 was used in Flow cytometry/Cell sorting to provide insight as to how HSC replacement can occur despite the residence of endogenous HSCs in niches.

Mon Nov 23 00:00:00 EST 2009

Bioinformatics

Protein Aliases: CD135; Fetal liver kinase 2; FL cytokine receptor; FLK-2; FLT-3; Fms-like tyrosine kinase 3; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; tyrosine-protein kinase FLT3; Tyrosine-protein kinase receptor flk-2

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Gene Aliases: B230315G04; CD135; Flk-2; Flk2; Flt-3; Flt3; Ly72; wmfl

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UniProt ID: (Mouse) Q00342

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Entrez Gene ID: (Mouse) 14255

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity cytokine receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups ubiquitin protein ligase binding protein complex binding glucocorticoid receptor binding phosphatidylinositol 3-kinase binding transmembrane signal receptor

Process(es)

leukocyte homeostasis positive regulation of protein phosphorylation myeloid progenitor cell differentiation lymphoid progenitor cell differentiation pro-B cell differentiation pro-T cell differentiation protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway negative regulation of cell proliferation phosphorylation peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway hemopoiesis lymphocyte differentiation cell differentiation B cell differentiation common myeloid progenitor cell proliferation negative regulation of B cell differentiation lymphocyte proliferation protein autophosphorylation cellular response to cytokine stimulus dendritic cell differentiation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD135 (Flt3) Monoclonal Antibody (A2F10), PE-Cyanine5, eBioscience™

CD135 (Flt3) Antibody (15-1351-82) in Flow

Product Details

15-1351-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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