Invitrogen

CD135 (Flt3) Monoclonal Antibody (A2F10), APC, eBioscience™

6 Published Figures

22 References

View all (47) FLT3 (CD135) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (6)

CD135 (Flt3) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 7

CD135 (Flt3) Antibody (17-1351-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD117 (c-Kit) PE (Product # 12-1171-82) and 0.5 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.5 µg of Anti-Mouse CD135 (Flt3) APC (right). Cells were also stained with Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) and Mouse Hematopoietic Lineage Cocktail eFluor® 450 (Product # 88-7772-72), and viable, lineage negative/low cells in the large scatter population were used for analysis.

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Product Details

17-1351-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A2F10

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717261

Product Specific Information

Description: The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.

Applications Reported: This A2F10 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression.

International journal of molecular sciences

Li N,Wang Y,Wang A,Zhang J,Jia C,Yu C,Song Z,Wang S,Liu L,Yi J,Bao Y,Huang Y,Sun L

Published figure using CD135 (Flt3) monoclonal antibody (Product # 17-1351-82) in Flow Cytometry

Fri Feb 03 00:00:00 EST 2023

STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression.

International journal of molecular sciences

Li N,Wang Y,Wang A,Zhang J,Jia C,Yu C,Song Z,Wang S,Liu L,Yi J,Bao Y,Huang Y,Sun L

Published figure using CD135 (Flt3) monoclonal antibody (Product # 17-1351-82) in Flow Cytometry

Fri Feb 03 00:00:00 EST 2023

A Myb enhancer-guided analysis of basophil and mast cell differentiation.

Nature communications

Matsumura T,Totani H,Gunji Y,Fukuda M,Yokomori R,Deng J,Rethnam M,Yang C,Tan TK,Karasawa T,Kario K,Takahashi M,Osato M,Sanda T,Suda T

17-1351-82 was used in Flow Cytometry to identify the Myb -68 enhancer, a regulatory element which marks basophils and mast cells, and show a population of granulocyte-macrophage progenitors with higher potential to differentiate into basophils and mast cells.

Fri Nov 18 00:00:00 EST 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD135 (Flt3) monoclonal antibody (Product # 17-1351-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Hlf Expression Marks Early Emergence of Hematopoietic Stem Cell Precursors With Adult Repopulating Potential and Fate.

Frontiers in cell and developmental biology

Tang W,He J,Huang T,Bai Z,Wang C,Wang H,Yang R,Ni Y,Hou J,Wang J,Zhou J,Yao Y,Gong Y,Hou S,Liu B,Lan Y

17-1351-82 was used in Flow cytometry/Cell sorting to develop an Hlf-tdTomato reporter mouse model and detected Hlf-tdTomato expression exclusively in the hematopoietic cells including part of the immunophenotypic CD45- and CD45+ pre-HSCs in the embryonic day (E) 10.5 AGM region.

Fri Oct 01 00:00:00 EDT 2021

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD135 (Flt3) monoclonal antibody (Product # 17-1351-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

SRC-3 Functions as a Coactivator of T-bet by Regulating the Maturation and Antitumor Activity of Natural Killer Cells.

Cancer immunology research

Hu M,Lu Y,Qi Y,Zhang Z,Wang S,Xu Y,Chen F,Tang Y,Chen S,Chen M,Du C,Shen M,Wang F,Su Y,Deng Y,Wang J

17-1351-82 was used in Flow Cytometry to study the role of SRC-3 as a coactivator of T-bet in natural killer-cell biology and indicate that targeting SRC-3 may be a promising strategy to increase the tumor surveillance function of natural killer cells.

Tue Sep 01 00:00:00 EDT 2020

Anti-FLT3 nanoparticles for acute myeloid leukemia: Preclinical pharmacology and pharmacokinetics.

Journal of controlled release : official journal of the Controlled Release Society

Park M,Vaikari VP,Lam AT,Zhang Y,MacKay JA,Alachkar H

17-1351-82 was used in Flow cytometry/Cell sorting to conclude that α-FLT3-A192 fusions appear to be a viable new modality for targeting FLT3 in AML and warrant further preclinical development to bring it into the clinic.

Mon Aug 10 00:00:00 EDT 2020

Zinc finger and BTB domain-containing protein 46 is essential for survival and proliferation of acute myeloid leukemia cell line but dispensable for normal hematopoiesis.

Chinese medical journal

Liu YY,Xiao FF,Yang BJ,Li X,Xu SN,Chen ZW,Li P,Huang YX,Fu XM,Huang XQ,Zheng GL,Chen JP,Hou Y

Published figure using CD135 (Flt3) monoclonal antibody (Product # 17-1351-82) in Flow Cytometry

Mon Jul 20 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD135 (Flt3) monoclonal antibody (Product # 17-1351-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Osterix-Cre marks distinct subsets of CD45- and CD45+ stromal populations in extra-skeletal tumors with pro-tumorigenic characteristics.

eLife

Ricci B,Tycksen E,Celik H,Belle JI,Fontana F,Civitelli R,Faccio R

17-1351 was used in Flow cytometry/Cell sorting to find Osx transcript and Osx protein expression early during hematopoiesis, in subsets of hematopoietic stem cells and multipotent progenitor populations.

Wed Aug 05 00:00:00 EDT 2020

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

Published figure using CD135 (Flt3) monoclonal antibody (Product # 17-1351-82) in Flow Cytometry

Tue Apr 16 00:00:00 EDT 2019

Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow.

Immunity

Walker JA,Clark PA,Crisp A,Barlow JL,Szeto A,Ferreira ACF,Rana BMJ,Jolin HE,Rodriguez-Rodriguez N,Sivasubramaniam M,Pannell R,Cruickshank J,Daly M,Haim-Vilmovsky L,Teichmann SA,McKenzie ANJ

Published figure using CD135 (Flt3) monoclonal antibody (Product # 17-1351-82) in Flow Cytometry

Tue Jul 16 00:00:00 EDT 2019

Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps.

Journal of visualized experiments : JoVE

Morganti C,Bonora M,Ito K

Published figure using CD135 (Flt3) monoclonal antibody (Product # 17-1351-82) in Flow Cytometry

Tue Jul 30 00:00:00 EDT 2019

Large-Scale Clonal Analysis Resolves Aging of the Mouse Hematopoietic Stem Cell Compartment.

Cell stem cell

Yamamoto R,Wilkinson AC,Ooehara J,Lan X,Lai CY,Nakauchi Y,Pritchard JK,Nakauchi H

17-1351 was used in Flow cytometry/Cell sorting to determine the age-related functional changes to the haematopoietic stem cell compartment.

Thu Apr 05 00:00:00 EDT 2018

The E-Id Protein Axis Specifies Adaptive Lymphoid Cell Identity and Suppresses Thymic Innate Lymphoid Cell Development.

Immunity

Miyazaki M,Miyazaki K,Chen K,Jin Y,Turner J,Moore AJ,Saito R,Yoshida K,Ogawa S,Rodewald HR,Lin YC,Kawamoto H,Murre C

17-1351 was used in Flow cytometry/Cell sorting to propose that the E-Id protein axis specifies innate and adaptive lymphoid cell fate.

Tue May 16 00:00:00 EDT 2017

Ubiquitination of hnRNPA1 by TRAF6 links chronic innate immune signaling with myelodysplasia.

Nature immunology

Fang J,Bolanos LC,Choi K,Liu X,Christie S,Akunuru S,Kumar R,Wang D,Chen X,Greis KD,Stoilov P,Filippi MD,Maciejewski JP,Garcia-Manero G,Weirauch MT,Salomonis N,Geiger H,Zheng Y,Starczynowski DT

17-1351 was used in Flow cytometry/Cell sorting to examine the significance of ubiquitin signalling in coordinating RNA processing by Toll-like receptor pathways during an immune response and in premalignant haematologic diseases.

Wed Feb 01 00:00:00 EST 2017

MLL-AF9- and HOXA9-mediated acute myeloid leukemia stem cell self-renewal requires JMJD1C.

The Journal of clinical investigation

Zhu N,Chen M,Eng R,DeJong J,Sinha AU,Rahnamay NF,Koche R,Al-Shahrour F,Minehart JC,Chen CW,Deshpande AJ,Xu H,Chu SH,Ebert BL,Roeder RG,Armstrong SA

17-1351 was used in Flow cytometry/Cell sorting to show that loss of JMJD1C substantially decreased LSC frequency and caused differentiation of MLL-AF9- and homeobox A9-driven leukemias.

Tue Mar 01 00:00:00 EST 2016

PML-RARA requires DNA methyltransferase 3A to initiate acute promyelocytic leukemia.

The Journal of clinical investigation

Cole CB,Verdoni AM,Ketkar S,Leight ER,Russler-Germain DA,Lamprecht TL,Demeter RT,Magrini V,Ley TJ

17-1351 was used in Flow cytometry/Cell sorting to suggest that PML-RARA requires DNMT3A to initiate acute promyelocytic leukemia in mice.

Fri Jan 01 00:00:00 EST 2016

Biliary repair and carcinogenesis are mediated by IL-33-dependent cholangiocyte proliferation.

The Journal of clinical investigation

Li J,Razumilava N,Gores GJ,Walters S,Mizuochi T,Mourya R,Bessho K,Wang YH,Glaser SS,Shivakumar P,Bezerra JA

17-1351 was used in Flow cytometry/Cell sorting to demonstrate the role of the Th2-promoting cytokine, IL-33, in cholangiocyte proliferation.

Tue Jul 01 00:00:00 EDT 2014

Eosinophils regulate peripheral B cell numbers in both mice and humans.

Journal of immunology (Baltimore, Md. : 1950)

Wong TW,Doyle AD,Lee JJ,Jelinek DF

17-1351 was used in Flow cytometry/Cell sorting to assess the effect of eosinophils on B cell survival, proliferation, and Ig secretion.

Tue Apr 15 00:00:00 EDT 2014

Chromosome instability underlies hematopoietic stem cell dysfunction and lymphoid neoplasia associated with impaired Fbw7-mediated cyclin E regulation.

Molecular and cellular biology

Siu KT,Xu Y,Swartz KL,Bhattacharyya M,Gurbuxani S,Hua Y,Minella AC

17-1351 was used in Flow cytometry/Cell sorting to investigate the contribution of individual substrate ubiquitination pathways to hematopoietic stem cell homeostasis, showing that impaired Fbw7-mediated cyclin E regulation leads to chromosome instability.

Mon Sep 01 00:00:00 EDT 2014

Usp16 contributes to somatic stem-cell defects in Down's syndrome.

Nature

Adorno M,Sikandar S,Mitra SS,Kuo A,Nicolis Di Robilant B,Haro-Acosta V,Ouadah Y,Quarta M,Rodriguez J,Qian D,Reddy VM,Cheshier S,Garner CC,Clarke MF

17-1351 was used in Flow cytometry/Cell sorting to demonstrate a role for USP16 in antagonising the self-renewal and senescence pathways in Down's syndrome.

Thu Sep 19 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD135; Fetal liver kinase 2; FL cytokine receptor; FLK-2; FLT-3; Fms-like tyrosine kinase 3; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; tyrosine-protein kinase FLT3; Tyrosine-protein kinase receptor flk-2

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Gene Aliases: B230315G04; CD135; Flk-2; Flk2; Flt-3; Flt3; Ly72; wmfl

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UniProt ID: (Mouse) Q00342

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Entrez Gene ID: (Mouse) 14255

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity cytokine receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups ubiquitin protein ligase binding protein complex binding glucocorticoid receptor binding phosphatidylinositol 3-kinase binding transmembrane signal receptor

Process(es)

leukocyte homeostasis positive regulation of protein phosphorylation myeloid progenitor cell differentiation lymphoid progenitor cell differentiation pro-B cell differentiation pro-T cell differentiation protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway negative regulation of cell proliferation phosphorylation peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway hemopoiesis lymphocyte differentiation cell differentiation B cell differentiation common myeloid progenitor cell proliferation negative regulation of B cell differentiation lymphocyte proliferation protein autophosphorylation cellular response to cytokine stimulus dendritic cell differentiation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD135 (Flt3) Monoclonal Antibody (A2F10), APC, eBioscience™

CD135 (Flt3) Antibody (17-1351-82) in Flow

Product Details

17-1351-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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