Invitrogen

CD135 (Flt3) Monoclonal Antibody (A2F10), PerCP-eFluor™ 710, eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (4)

CD135 (Flt3) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD135 (Flt3) Antibody (46-1351-82) in Flow

Staining of BALB/c bone marrow cells with Mouse Hematopoietic Lineage Cocktail eFluor® 450 (Product # 88-7772-72), Anti-Mouse CD117 APC (Product # 17-1171-82) and 0.5 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or 0.5 µg of Anti-Mouse CD135 (Flt3) PerCP-eFluor® 710 (right). Lineage negative/low, viable cells, as determined by Fixable Viability Dye eFluor® 506, were used for analysis.

Product Image Gallery

Product Details

46-1351-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Mouse

Published species

Hamster, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A2F10

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10733393

Product Specific Information

Description: The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.

Applications Reported: This A2F10 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression.

International journal of molecular sciences

Li N,Wang Y,Wang A,Zhang J,Jia C,Yu C,Song Z,Wang S,Liu L,Yi J,Bao Y,Huang Y,Sun L

Published figure using CD135 (Flt3) monoclonal antibody (Product # 46-1351-82) in Flow Cytometry

Fri Feb 03 00:00:00 EST 2023

The microbiota regulates hematopoietic stem cell fate decisions by controlling iron availability in bone marrow.

Cell stem cell

Zhang D,Gao X,Li H,Borger DK,Wei Q,Yang E,Xu C,Pinho S,Frenette PS

46-1351-82 was used in Flow cytometry/Cell sorting to reveal an intricate interplay between the microbiota, macrophages, and iron, and their essential roles in regulating critical HSC fate decisions under stress.

Thu Feb 03 00:00:00 EST 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD135 (Flt3) monoclonal antibody (Product # 46-1351-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Microbiota triggers STING-type I IFN-dependent monocyte reprogramming of the tumor microenvironment.

Cell

Lam KC,Araya RE,Huang A,Chen Q,Di Modica M,Rodrigues RR,Lopès A,Johnson SB,Schwarz B,Bohrnsen E,Cogdill AP,Bosio CM,Wargo JA,Lee MP,Goldszmid RS

46-1351-82 was used in Flow cytometry/Cell sorting to uncover a mechanistic link between the microbiota and the innate TME that can be harnessed to improve cancer therapies.

Thu Oct 14 00:00:00 EDT 2021

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD135 (Flt3) monoclonal antibody (Product # 46-1351-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Zinc finger and BTB domain-containing protein 46 is essential for survival and proliferation of acute myeloid leukemia cell line but dispensable for normal hematopoiesis.

Chinese medical journal

Liu YY,Xiao FF,Yang BJ,Li X,Xu SN,Chen ZW,Li P,Huang YX,Fu XM,Huang XQ,Zheng GL,Chen JP,Hou Y

46-1351 was used in Flow cytometry/Cell sorting to show Zinc finger and BTB domain-containing protein 46 (Zbtb46) is essential in acute myeloid leukemia cells for survival and proliferation.

Mon Jul 20 00:00:00 EDT 2020

Zinc finger and BTB domain-containing protein 46 is essential for survival and proliferation of acute myeloid leukemia cell line but dispensable for normal hematopoiesis.

Chinese medical journal

Liu YY,Xiao FF,Yang BJ,Li X,Xu SN,Chen ZW,Li P,Huang YX,Fu XM,Huang XQ,Zheng GL,Chen JP,Hou Y

46-1351 was used in Flow cytometry/Cell sorting to show Zinc finger and BTB domain-containing protein 46 (Zbtb46) is essential in acute myeloid leukemia cells for survival and proliferation.

Mon Jul 20 00:00:00 EDT 2020

Development of an inexpensive Raman-compatible substrate for the construction of a microarray screening platform.

The Analyst

Pastrana-Otero I,Majumdar S,Gilchrist AE,Gorman BL,Harley BAC,Kraft ML

46-1351-82 was used in Flow Cytometry to show that the spectra acquired from individual living cells on functionalized PA gels attached to our custom substrates were of sufficient quality to enable accurate identification of cell phenotypes using PLS-DA models of the cell spectra.

Mon Oct 26 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD135 (Flt3) monoclonal antibody (Product # 46-1351-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Proteomic analysis of young and old mouse hematopoietic stem cells and their progenitors reveals post-transcriptional regulation in stem cells.

eLife

Zaro BW,Noh JJ,Mascetti VL,Demeter J,George B,Zukowska M,Gulati GS,Sinha R,Flynn RA,Banuelos A,Zhang A,Wilkinson AC,Jackson P,Weissman IL

46-1351 was used in Flow cytometry/Cell sorting to report a proteomics resource from mass spectrometry of mouse young adult and old adult mouse hematopoietic stem cells, multipotent progenitors and oligopotent progenitors; 12 cell types in total.

Wed Nov 25 00:00:00 EST 2020

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

Published figure using CD135 (Flt3) monoclonal antibody (Product # 46-1351-82) in Flow Cytometry

Tue Apr 16 00:00:00 EDT 2019

Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow.

Immunity

Walker JA,Clark PA,Crisp A,Barlow JL,Szeto A,Ferreira ACF,Rana BMJ,Jolin HE,Rodriguez-Rodriguez N,Sivasubramaniam M,Pannell R,Cruickshank J,Daly M,Haim-Vilmovsky L,Teichmann SA,McKenzie ANJ

46-1351 was used in Flow cytometry/Cell sorting to identify a discrete ILC2-committed population and delineated transition states between early progenitors and a highly heterogeneous ILC1, ILC3, and NK precursor cell cluster.

Tue Jul 16 00:00:00 EDT 2019

Mutations in topoisomerase IIβ result in a B cell immunodeficiency.

Nature communications

Broderick L,Yost S,Li D,McGeough MD,Booshehri LM,Guaderrama M,Brydges SD,Kucharova K,Patel NC,Harr M,Hakonarson H,Zackai E,Cowell IG,Austin CA,Hügle B,Gebauer C,Zhang J,Xu X,Wang J,Croker BA,Frazer KA,Putnam CD,Hoffman HM

46-1351-82 was used in Flow Cytometry to identify mutations in type II topoisomerase as a driver of defective proliferation and survival of B2 cells, leading to a B cell immunodeficiency.

Tue Aug 13 00:00:00 EDT 2019

Phc2 controls hematopoietic stem and progenitor cell mobilization from bone marrow by repressing Vcam1 expression.

Nature communications

Bae J,Choi SP,Isono K,Lee JY,Park SW,Choi CY,Han J,Kim SH,Lee HH,Park K,Jin HY,Lee SJ,Park CG,Koseki H,Lee YS,Chun T

46-1351-82 was used in Flow cytometry/Cell sorting to determine the role of PHc2 in controlling the mobilisation of hematopoietic stem and progenitor cells.

Fri Aug 02 00:00:00 EDT 2019

FateID infers cell fate bias in multipotent progenitors from single-cell RNA-seq data.

Nature methods

Herman JS,Grün D

46-1351-82 was used in Flow Cytometry to understand stem cell differentiation along multiple lineages, it is necessary to resolve heterogeneous cellular states and the ancestral relationships between them.

Tue May 01 00:00:00 EDT 2018

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

46-1351 was used in Flow Cytometry to indicate that the VHL-HIF-glycolysis axis is essential for the late-stage maturation and function of ILC2s via targeting IL-33-ST2 pathway.

Tue Feb 20 00:00:00 EST 2018

Large-Scale Clonal Analysis Resolves Aging of the Mouse Hematopoietic Stem Cell Compartment.

Cell stem cell

Yamamoto R,Wilkinson AC,Ooehara J,Lan X,Lai CY,Nakauchi Y,Pritchard JK,Nakauchi H

46-1351 was used in Flow cytometry/Cell sorting to determine the age-related functional changes to the haematopoietic stem cell compartment.

Thu Apr 05 00:00:00 EDT 2018

Notch Signaling Facilitates In Vitro Generation of Cross-Presenting Classical Dendritic Cells.

Cell reports

Kirkling ME,Cytlak U,Lau CM,Lewis KL,Resteu A,Khodadadi-Jamayran A,Siebel CW,Salmon H,Merad M,Tsirigos A,Collin M,Bigley V,Reizis B

46-1351 was used in Flow cytometry/Cell sorting to examine the role of Notch signalling in the in vitro generation of classical dendritic cells.

Tue Jun 19 00:00:00 EDT 2018

Human umbilical cord blood-borne fibroblasts contain marrow niche precursors that form a bone/marrow organoid in vivo.

Development (Cambridge, England)

Pievani A,Sacchetti B,Corsi A,Rambaldi B,Donsante S,Scagliotti V,Vergani P,Remoli C,Biondi A,Robey PG,Riminucci M,Serafini M

46-1351 was used in Flow cytometry/Cell sorting to show that human umbilical cord blood-borne fibroblasts are able to recapitulate the conditions by which the bone marrow microenvironment is formed and establish complete hematopoietic stem cell niches.

Wed Mar 15 00:00:00 EDT 2017

Frizzled-6 Regulates Hematopoietic Stem/Progenitor Cell Survival and Self-Renewal.

Journal of immunology (Baltimore, Md. : 1950)

Abidin BM,Owusu Kwarteng E,Heinonen KM

46-1351 was used in Flow cytometry/Cell sorting to reveal that Fzd6 has an essential role in HSPC maintenance and survival.

Tue Sep 01 00:00:00 EDT 2015

Clonal analysis unveils self-renewing lineage-restricted progenitors generated directly from hematopoietic stem cells.

Cell

Yamamoto R,Morita Y,Ooehara J,Hamanaka S,Onodera M,Rudolph KL,Ema H,Nakauchi H

46-1351 was used in Flow cytometry/Cell sorting to identify multipotent progenitors of myeloid cells and study differentiation pathways giving rise to common myeloid cells.

Thu Aug 29 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD135; Fetal liver kinase 2; FL cytokine receptor; FLK-2; FLT-3; Fms-like tyrosine kinase 3; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; tyrosine-protein kinase FLT3; Tyrosine-protein kinase receptor flk-2

View more View less

Gene Aliases: B230315G04; CD135; Flk-2; Flk2; Flt-3; Flt3; Ly72; wmfl

View more View less

UniProt ID: (Mouse) Q00342

View more View less

Entrez Gene ID: (Mouse) 14255

View more View less

Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity cytokine receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups ubiquitin protein ligase binding protein complex binding glucocorticoid receptor binding phosphatidylinositol 3-kinase binding transmembrane signal receptor

Process(es)

leukocyte homeostasis positive regulation of protein phosphorylation myeloid progenitor cell differentiation lymphoid progenitor cell differentiation pro-B cell differentiation pro-T cell differentiation protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway negative regulation of cell proliferation phosphorylation peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway hemopoiesis lymphocyte differentiation cell differentiation B cell differentiation common myeloid progenitor cell proliferation negative regulation of B cell differentiation lymphocyte proliferation protein autophosphorylation cellular response to cytokine stimulus dendritic cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD135 (Flt3) Monoclonal Antibody (A2F10), PerCP-eFluor™ 710, eBioscience™

CD135 (Flt3) Antibody (46-1351-82) in Flow

Product Details

46-1351-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!