Invitrogen

CD138 (Syndecan-1) Monoclonal Antibody (DL-101), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9 Published Figures

13 References

View all (80) CD138 (Syndecan-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 11

CD138 (Syndecan-1) Antibody (12-1389-42) in Flow

Staining of the U266 cell line with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD138 (Syndecan-1) PE (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 660, were used for analysis.

Product Image Gallery

Product Details

12-1389-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DL-101

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10548508

Product Specific Information

Description: The DL-101 monoclonal antibody reacts with human CD138, also known as syndecan-1. CD138 is a transmembrane protein containing chondroitin sulfate and heparin sulfate moieties responsible for binding to the extracellular matrix components. CD138 is not expressed by mature B cells but is present on pre-B cells and the finally differentiated plasma cells.

Applications Reported: DL-101 has been reported for use in flow cytometric analysis.

Applications Tested: This DL-101 antibody has been pre-titrated and tested by flow cytometric analysis of the U266 cell line. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Macrophages drive KSHV B cell latency.

Cell reports

Szymula A,Samayoa-Reyes G,Ogolla S,Liu B,Li S,George A,Van Sciver N,Rochford R,Simas JP,Kaye KM

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 12-1389-42) in Flow Cytometry

Tue Jul 25 00:00:00 EDT 2023

Attenuated Dengue virus PV001-DV induces oncolytic tumor cell death and potent immune responses.

Journal of translational medicine

Goldufsky JW,Daniels P,Williams MD,Gupta K,Lyday B,Chen T,Singh G,Kaufman HL,Zloza A,Marzo AL

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 12-1389-42) in Flow Cytometry

Wed Jul 19 00:00:00 EDT 2023

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection.

PLoS pathogens

Akidil E,Albanese M,Buschle A,Ruhle A,Pich D,Keppler OT,Hammerschmidt W

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 12-1389-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis.

Immunity

Castro-Dopico T,Dennison TW,Ferdinand JR,Mathews RJ,Fleming A,Clift D,Stewart BJ,Jing C,Strongili K,Labzin LI,Monk EJM,Saeb-Parsy K,Bryant CE,Clare S,Parkes M,Clatworthy MR

12-1389-42 was used in Flow cytometry/Cell sorting to understand the mechanisms whereby changes in FcγRIIA affinity would affect inflammation in an IgA-dominated organ.

Tue Apr 16 00:00:00 EDT 2019

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 12-1389-42) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

Syndecan-1 is a novel molecular marker for triple negative inflammatory breast cancer and modulates the cancer stem cell phenotype via the IL-6/STAT3, Notch and EGFR signaling pathways.

Molecular cancer

Ibrahim SA,Gadalla R,El-Ghonaimy EA,Samir O,Mohamed HT,Hassan H,Greve B,El-Shinawi M,Mohamed MM,Götte M

12-1389 was used in Flow cytometry/Cell sorting to identify Syndecan-1 as a molecular marker in IBC patients, and demonstrate its role in fine-tuning IL-6/STAT3, Notch and EGFR signaling pathways.

Tue Mar 07 00:00:00 EST 2017

CD138 and CD31 Double-Positive Cells Comprise the Functional Antibody-Secreting Plasma Cell Compartment in Primate Bone Marrow.

Frontiers in immunology

Martinez-Murillo P,Pramanik L,Sundling C,Hultenby K,Wretenberg P,Spångberg M,Karlsson Hedestam GB

12-1389 was used in Flow cytometry/Cell sorting to characterise bone-marrow resident rhesus plasma cell phenotype and function.

Fri Jul 22 00:00:00 EDT 2016

Mitochondrial-Targeted Decyl-Triphenylphosphonium Enhances 2-Deoxy-D-Glucose Mediated Oxidative Stress and Clonogenic Killing of Multiple Myeloma Cells.

PloS one

Schibler J,Tomanek-Chalkley AM,Reedy JL,Zhan F,Spitz DR,Schultz MK,Goel A

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 12-1389-42) in Flow Cytometry

Thu Jul 06 00:00:00 EDT 2017

Bone marrow plasma cells are a primary source of serum HIV-1-specific antibodies in chronically infected individuals.

Journal of immunology (Baltimore, Md. : 1950)

Montezuma-Rusca JM,Moir S,Kardava L,Buckner CM,Louie A,Kim LJ,Santich BH,Wang W,Fankuchen OR,Diaz G,Daub JR,Rosenzweig SD,Chun TW,Li Y,Braylan RC,Calvo KR,Fauci AS

12-1389 was used in Flow cytometry/Cell sorting to demonstrate that bone marrow plasma cells are a primary source for circulating HIV-1-specific antibodies in HIV-1-infected individuals.

Sun Mar 15 00:00:00 EDT 2015

A CD8 T cell/indoleamine 2,3-dioxygenase axis is required for mesenchymal stem cell suppression of human systemic lupus erythematosus.

Arthritis & rheumatology (Hoboken, N.J.)

Wang D,Feng X,Lu L,Konkel JE,Zhang H,Chen Z,Li X,Gao X,Lu L,Shi S,Chen W,Sun L

12-1389 was used in Flow cytometry/Cell sorting to highlight a previously unrecognised CD8+ T cell/IFNγ/IDO axis that mediates the therapeutic effects of allogeneic MSCs in lupus patients.

Fri Aug 01 00:00:00 EDT 2014

Flow cytometry assessment of in vitro generated CD138+ human plasma cells.

BioMed research international

Itoua Maïga R,Lemieux J,Roy A,Simard C,Néron S

12-1389 was used in Flow cytometry/Cell sorting to assess the effectiveness of in vitro differentiation techniques to produce plasma cells from B lymphocytes.

Wed May 13 00:00:00 EDT 2015

Dynamics of memory B-cell populations in blood, lymph nodes, and bone marrow during antiretroviral therapy and envelope boosting in simian immunodeficiency virus SIVmac251-infected rhesus macaques.

Journal of virology

Demberg T,Brocca-Cofano E,Xiao P,Venzon D,Vargas-Inchaustegui D,Lee EM,Kalisz I,Kalyanaraman VS,Dipasquale J,McKinnon K,Robert-Guroff M

12-1389 was used in Flow cytometry/Cell sorting to investigate memory B-cell subpopulations and plasma cells/plasmablasts of rhesus macaques during, and upon release of, antiretoviral treatment, showing that long-term memory does not form.

Sat Dec 01 00:00:00 EST 2012

Long-lived plasma cells from human small intestine biopsies secrete immunoglobulins for many weeks in vitro.

Journal of immunology (Baltimore, Md. : 1950)

Mesin L,Di Niro R,Thompson KM,Lundin KE,Sollid LM

12-1389 was used in Flow cytometry/Cell sorting to demonstrate that the human small intestine harbors nonproliferating plasma cells that are instructed by the microenvironment for prolonged survival and Ab secretion.

Thu Sep 15 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD antigen 138; CD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; sCD138; soluble CD138; SYND1; syndecan proteoglycan 1; Syndecan-1; Syndecan1

View more View less

Gene Aliases: CD138; SDC; SDC1; SYND1; syndecan

View more View less

UniProt ID: (Human) P18827

View more View less

Entrez Gene ID: (Human) 6382

View more View less

Function(s)

glycoprotein binding protein binding protein C-terminus binding transmembrane signal receptor

Process(es)

retinoid metabolic process ureteric bud development glycosaminoglycan biosynthetic process glycosaminoglycan catabolic process inflammatory response response to toxic substance cell migration glycosaminoglycan metabolic process wound healing lipoprotein metabolic process odontogenesis response to hydrogen peroxide myoblast development response to glucocorticoid response to cAMP response to calcium ion striated muscle cell development Sertoli cell development canonical Wnt signaling pathway positive regulation of exosomal secretion positive regulation of extracellular exosome assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD138 (Syndecan-1) Monoclonal Antibody (DL-101), PE, eBioscience™

CD138 (Syndecan-1) Antibody (12-1389-42) in Flow

Product Details

12-1389-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!