Invitrogen

CD140a (PDGFRA) Monoclonal Antibody (APA5), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

57 Published Figures

56 References

View all (78) PDGFRA (CD140a) antibodies

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Datasheet

Protocols

Questions & Answers

CD140a (PDGFRA) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 59

CD140a (PDGFRA) Antibody (25-1401-82) in Flow

Staining of NIH/3T3 cells with 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (blue histogram) or 0.06 µg of Anti-Mouse CD140a (PDGF Receptor a) PE-Cyanine7 (purple histogram). Total viable cells were used for analysis.

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Product Details

25-1401-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Flow Cytometry (Flow)

0.125 µg/test

View 54 publications 54 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

APA5

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573400

Product Specific Information

Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Distinct niche structures and intrinsic programs of fallopian tube and ovarian surface epithelial cells.

iScience

Qin G,Park ES,Chen X,Han S,Xiang D,Ren F,Liu G,Chen H,Yuan GC,Li Z

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Fri Jan 20 00:00:00 EST 2023

Stromal niche inflammation mediated by IL-1 signalling is a targetable driver of haematopoietic ageing.

Nature cell biology

Mitchell CA,Verovskaya EV,Calero-Nieto FJ,Olson OC,Swann JW,Wang X,Hérault A,Dellorusso PV,Zhang SY,Svendsen AF,Pietras EM,Bakker ST,Ho TT,Göttgens B,Passegué E

25-1401-82 was used in Flow cytometry/Cell sorting to characterize the inflammatory milieu that drives both niche and haematopoietic remodelling.

Sun Jan 01 00:00:00 EST 2023

The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency.

Nature communications

Athanasouli P,Balli M,De Jaime-Soguero A,Boel A,Papanikolaou S,van der Veer BK,Janiszewski A,Vanhessche T,Francis A,El Laithy Y,Nigro AL,Aulicino F,Koh KP,Pasque V,Cosma MP,Verfaillie C,Zwijsen A,Heindryckx B,Nikolaou C,Lluis F

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Fri Mar 03 00:00:00 EST 2023

Inhibition of antiapoptotic BCL-2 proteins with ABT-263 induces fibroblast apoptosis, reversing persistent pulmonary fibrosis.

JCI insight

Cooley JC,Javkhlan N,Wilson JA,Foster DG,Edelman BL,Ortiz LA,Schwartz DA,Riches DW,Redente EF

25-1401-82 was used in Flow cytometry/Cell sorting to conclude that, by targeting profibrotic fibroblasts, ABT-263 offers a promising therapeutic option for progressive fibrosing interstitial lung diseases (PF-ILDs).

Wed Feb 08 00:00:00 EST 2023

An estrogen-sensitive fibroblast population drives abdominal muscle fibrosis in an inguinal hernia mouse model.

JCI insight

Potluri T,Taylor MJ,Stulberg JJ,Lieber RL,Zhao H,Bulun SE

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Apr 19 00:00:00 EDT 2022

Hematopoietic-Mesenchymal Signals Regulate the Properties of Mesenchymal Stem Cells.

International journal of molecular sciences

Kanazawa S,Okada H,Riu D,Mabuchi Y,Akazawa C,Iwata J,Hoshi K,Hikita A

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Jul 26 00:00:00 EDT 2022

PEAR1 regulates expansion of activated fibroblasts and deposition of extracellular matrix in pulmonary fibrosis.

Nature communications

Geng Y,Li L,Yan J,Liu K,Yang A,Zhang L,Shen Y,Gao H,Wu X,Noth I,Huang Y,Liu J,Fan X

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Sat Nov 19 00:00:00 EST 2022

Lamin A/C-dependent chromatin architecture safeguards naïve pluripotency to prevent aberrant cardiovascular cell fate and function.

Nature communications

Wang Y,Elsherbiny A,Kessler L,Cordero J,Shi H,Serke H,Lityagina O,Trogisch FA,Mohammadi MM,El-Battrawy I,Backs J,Wieland T,Heineke J,Dobreva G

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Fri Nov 04 00:00:00 EDT 2022

Evidence for oligodendrocyte progenitor cell heterogeneity in the adult mouse brain.

Scientific reports

Beiter RM,Rivet-Noor C,Merchak AR,Bai R,Johanson DM,Slogar E,Sol-Church K,Overall CC,Gaultier A

25-1401-82 was used in Flow cytometry/Cell sorting to investigate the exact mechanisms through which Oligodendrocyte progenitor cells might influence brain function.

Thu Jul 28 00:00:00 EDT 2022

IGF1R controls mechanosignaling in myofibroblasts required for pulmonary alveologenesis.

JCI insight

He H,Snowball J,Sun F,Na CL,Whitsett JA

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Mon Mar 22 00:00:00 EDT 2021

Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer.

Nature communications

Nishina T,Deguchi Y,Ohshima D,Takeda W,Ohtsuka M,Shichino S,Ueha S,Yamazaki S,Kawauchi M,Nakamura E,Nishiyama C,Kojima Y,Adachi-Akahane S,Hasegawa M,Nakayama M,Oshima M,Yagita H,Shibuya K,Mikami T,Inohara N,Matsushima K,Tada N,Nakano H

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Fri Apr 16 00:00:00 EDT 2021

Gain-of-function p53 protein transferred via small extracellular vesicles promotes conversion of fibroblasts to a cancer-associated phenotype.

Cell reports

Ma S,McGuire MH,Mangala LS,Lee S,Stur E,Hu W,Bayraktar E,Villar-Prados A,Ivan C,Wu SY,Yokoi A,Dasari SK,Jennings NB,Liu J,Lopez-Berestein G,Ram P,Sood AK

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Feb 09 00:00:00 EST 2021

Extracellular vesicles synchronize cellular phenotypes of differentiating cells.

Journal of extracellular vesicles

Minakawa T,Matoba T,Ishidate F,Fujiwara TK,Takehana S,Tabata Y,Yamashita JK

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Mesothelial cells are not a source of adipocytes in mice.

Cell reports

Westcott GP,Emont MP,Li J,Jacobs C,Tsai L,Rosen ED

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Jul 13 00:00:00 EDT 2021

Heterogeneity of murine periosteum progenitors involved in fracture healing.

eLife

Matthews BG,Novak S,Sbrana FV,Funnell JL,Cao Y,Buckels EJ,Grcevic D,Kalajzic I

25-1401-82 was used in Flow Cytometry to demonstrate that the periosteum is highly enriched with skeletal progenitor cells, and there is heterogeneity in the populations of cells that contribute to mature lineages during periosteal fracture healing.

Tue Feb 09 00:00:00 EST 2021

Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice.

Scientific reports

Wilm TP,Tanton H,Mutter F,Foisor V,Middlehurst B,Ward K,Benameur T,Hastie N,Wilm B

25-1401-82 was used in Flow cytometry/Cell sorting to determine the spatio-temporal contribution of Wt1-expressing cells to the adult and postnatal intestine and peritoneal body wall, including the mesentery.

Thu Aug 05 00:00:00 EDT 2021

TNF-α-induced alterations in stromal progenitors enhance leukemic stem cell growth via CXCR2 signaling.

Cell reports

Agarwal P,Li H,Choi K,Hueneman K,He J,Welner RS,Starczynowski DT,Bhatia R

25-1401-82 was used in Flow Cytometry to find that TNF-α-mediated alterations in CML BM stromal niches enhance support of LSC maintenance and growth via CXCL1-CXCR2 signaling and that CXCR2 inhibition effectively depletes CML LSCs.

Tue Jul 13 00:00:00 EDT 2021

Hyaluronan Degradation by Cemip Regulates Host Defense against Staphylococcus aureus Skin Infection.

Cell reports

Dokoshi T,Zhang LJ,Li F,Nakatsuji T,Butcher A,Yoshida H,Shimoda M,Okada Y,Gallo RL

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Jan 07 00:00:00 EST 2020

Tissue-Resident PDGFRα⁺ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner.

Cell reports

Santini MP,Malide D,Hoffman G,Pandey G,D'Escamard V,Nomura-Kitabayashi A,Rovira I,Kataoka H,Ochando J,Harvey RP,Finkel T,Kovacic JC

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis.

JCI insight

Redente EF,Chakraborty S,Sajuthi S,Black BP,Edelman BL,Seibold MA,Riches DW

25-1401 was used in Flow cytometry/Cell sorting to test the hypothesis that the development of resistance to Fas-induced apoptosis by lung fibroblasts and myofibroblasts contibributes to their accumulation in the distal lung tissues of IPF patients.

Tue Dec 08 00:00:00 EST 2020

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis.

JCI insight

Redente EF,Chakraborty S,Sajuthi S,Black BP,Edelman BL,Seibold MA,Riches DW

Tue Dec 08 00:00:00 EST 2020

Adipocyte Piezo1 mediates obesogenic adipogenesis through the FGF1/FGFR1 signaling pathway in mice.

Nature communications

Wang S,Cao S,Arhatte M,Li D,Shi Y,Kurz S,Hu J,Wang L,Shao J,Atzberger A,Wang Z,Wang C,Zang W,Fleming I,Wettschureck N,Honoré E,Offermanns S

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Fri May 08 00:00:00 EDT 2020

Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells.

PloS one

Falero-Perez J,Sheibani N,Sorenson CM

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Fri Jul 31 00:00:00 EDT 2020

Cardiac fibroblast proliferation rates and collagen expression mature early and are unaltered with advancing age.

JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Thu Dec 17 00:00:00 EST 2020

Phosphatase SHP1 impedes mesenchymal stromal cell immunosuppressive capacity modulated by JAK1/STAT3 and P38 signals.

Cell & bioscience

Jiang M,Ye J,Wang X,Li N,Wang Y,Shi Y

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Thu Apr 14 00:00:00 EDT 2022

Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells.

Cell stem cell

Agarwal P,Isringhausen S,Li H,Paterson AJ,He J,Gomariz Á,Nagasawa T,Nombela-Arrieta C,Bhatia R

25-1401-82 was used in Flow Cytometry to highlight a critical role of niche-specific effects of Cxcl12 expression in maintaining quiescence of TKI-resistant LSC populations.

Thu May 02 00:00:00 EDT 2019

PDGFRA defines the mesenchymal stem cell Kaposi's sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environment.

PLoS pathogens

Naipauer J,Rosario S,Gupta S,Premer C,Méndez-Solís O,Schlesinger M,Ponzinibbio V,Jain V,Gay L,Renne R,Chan HL,Morey L,Salyakina D,Abba M,Williams S,Hare JM,Goldschmidt-Clermont PJ,Mesri EA

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

Dual lineage tracing identifies intermediate mesenchymal stage for endocardial contribution to fibroblasts, coronary mural cells, and adipocytes.

The Journal of biological chemistry

Huang X,Feng T,Jiang Z,Meng J,Kou S,Lu Z,Chen W,Lin CP,Zhou B,Zhang H

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Fri May 31 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Mar 05 00:00:00 EST 2019

Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy.

Nature communications

Welc SS,Flores I,Wehling-Henricks M,Ramos J,Wang Y,Bertoni C,Tidball JG

25-1401 was used in Flow cytometry/Cell sorting to study the effect of expression of a therapeutic transgene in leukocyte progenitors that invade muscle on selective and timely delivery to diseased muscle.

Wed Jun 26 00:00:00 EDT 2019

Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.

Nature medicine

Maryanovich M,Zahalka AH,Pierce H,Pinho S,Nakahara F,Asada N,Wei Q,Wang X,Ciero P,Xu J,Leftin A,Frenette PS

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

SCL/TAL1 cooperates with Polycomb RYBP-PRC1 to suppress alternative lineages in blood-fated cells.

Nature communications

Chagraoui H,Kristiansen MS,Ruiz JP,Serra-Barros A,Richter J,Hall-Ponselé E,Gray N,Waithe D,Clark K,Hublitz P,Repapi E,Otto G,Sopp P,Taylor S,Thongjuea S,Vyas P,Porcher C

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Dec 18 00:00:00 EST 2018

FZD4 Marks Lateral Plate Mesoderm and Signals with NORRIN to Increase Cardiomyocyte Induction from Pluripotent Stem Cell-Derived Cardiac Progenitors.

Stem cell reports

Yoon C,Song H,Yin T,Bausch-Fluck D,Frei AP,Kattman S,Dubois N,Witty AD,Hewel JA,Guo H,Emili A,Wollscheid B,Keller G,Zandstra PW

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Jan 09 00:00:00 EST 2018

Pdgfra marks a cellular lineage with distinct contributions to myofibroblasts in lung maturation and injury response.

eLife

Li R,Bernau K,Sandbo N,Gu J,Preissl S,Sun X

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Sep 04 00:00:00 EDT 2018

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

25-1401 was used in Flow cytometry/Cell sorting to study paediatric acute myeloid leukaemia biology, and provide murine models that offer the potential for developing paediatric specific therapeutic strategies.

Tue Dec 11 00:00:00 EST 2018

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

Tue Dec 11 00:00:00 EST 2018

Mesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitis.

Cell death & disease

Feng Y,Liao Y,Huang W,Lai X,Luo J,Du C,Lin J,Zhang Z,Qiu D,Liu Q,Shen H,Xiang AP,Zhang Q

25-1401 was used in Flow cytometry/Cell sorting to study the role of matrix Gla protein as a novel mediator of mesenchymal stromal cell-mediated immunomodulation in treating Crohn's disease.

Thu Jun 07 00:00:00 EDT 2018

Gli1 identifies osteogenic progenitors for bone formation and fracture repair.

Nature communications

Shi Y,He G,Lee WC,McKenzie JA,Silva MJ,Long F

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Mon Dec 11 00:00:00 EST 2017

Pericytes regulate VEGF-induced endothelial sprouting through VEGFR1.

Nature communications

Eilken HM,Diéguez-Hurtado R,Schmidt I,Nakayama M,Jeong HW,Arf H,Adams S,Ferrara N,Adams RH

25-1401 was used in Flow cytometry/Cell sorting to show that pericytes promote endothelial sprouting in the postnatal retinal vasculature.

Fri Nov 17 00:00:00 EST 2017

Cardiac Fibro-Adipocyte Progenitors Express Desmosome Proteins and Preferentially Differentiate to Adipocytes Upon Deletion of the Desmoplakin Gene.

Circulation research

Lombardi R,Chen SN,Ruggiero A,Gurha P,Czernuszewicz GZ,Willerson JT,Marian AJ

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Fri Jun 24 00:00:00 EDT 2016

Genetic lineage tracing defines myofibroblast origin and function in the injured heart.

Nature communications

Kanisicak O,Khalil H,Ivey MJ,Karch J,Maliken BD,Correll RN,Brody MJ,J Lin SC,Aronow BJ,Tallquist MD,Molkentin JD

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Fri Jul 22 00:00:00 EDT 2016

mTOR inhibition and BMP signaling act synergistically to reduce muscle fibrosis and improve myofiber regeneration.

JCI insight

Agarwal S,Cholok D,Loder S,Li J,Breuler C,Chung MT,Sung HH,Ranganathan K,Habbouche J,Drake J,Peterson J,Priest C,Li S,Mishina Y,Levi B

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Thu Dec 08 00:00:00 EST 2016

Drug-based modulation of endogenous stem cells promotes functional remyelination in vivo.

Nature

Najm FJ,Madhavan M,Zaremba A,Shick E,Karl RT,Factor DC,Miller TE,Nevin ZS,Kantor C,Sargent A,Quick KL,Schlatzer DM,Tang H,Papoian R,Brimacombe KR,Shen M,Boxer MB,Jadhav A,Robinson AP,Podojil JR,Miller SD,Miller RH,Tesar PJ

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Thu Jun 11 00:00:00 EDT 2015

The Isl1/Ldb1 Complex Orchestrates Genome-wide Chromatin Organization to Instruct Differentiation of Multipotent Cardiac Progenitors.

Cell stem cell

Caputo L,Witzel HR,Kolovos P,Cheedipudi S,Looso M,Mylona A,van IJcken WF,Laugwitz KL,Evans SM,Braun T,Soler E,Grosveld F,Dobreva G

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Thu Sep 03 00:00:00 EDT 2015

Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle.

The Journal of clinical investigation

Quattrocelli M,Swinnen M,Giacomazzi G,Camps J,Barthélemy I,Ceccarelli G,Caluwé E,Grosemans H,Thorrez L,Pelizzo G,Muijtjens M,Verfaillie CM,Blot S,Janssens S,Sampaolesi M

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

IKKβ acts as a tumor suppressor in cancer-associated fibroblasts during intestinal tumorigenesis.

The Journal of experimental medicine

Pallangyo CK,Ziegler PK,Greten FR

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Mon Dec 14 00:00:00 EST 2015

Brg1 modulates enhancer activation in mesoderm lineage commitment.

Development (Cambridge, England)

Alexander JM,Hota SK,He D,Thomas S,Ho L,Pennacchio LA,Bruneau BG

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Wed Apr 15 00:00:00 EDT 2015

Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.

Stem cell reports

Djelloul M,Holmqvist S,Boza-Serrano A,Azevedo C,Yeung MS,Goldwurm S,Frisén J,Deierborg T,Roybon L

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Aug 11 00:00:00 EDT 2015

Cardiogenic genes expressed in cardiac fibroblasts contribute to heart development and repair.

Circulation research

Furtado MB,Costa MW,Pranoto EA,Salimova E,Pinto AR,Lam NT,Park A,Snider P,Chandran A,Harvey RP,Boyd R,Conway SJ,Pearson J,Kaye DM,Rosenthal NA

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Fri Apr 25 00:00:00 EDT 2014

Recruitment of bone marrow-derived cells to periodontal tissue defects.

Frontiers in cell and developmental biology

Kimura Y,Komaki M,Iwasaki K,Sata M,Izumi Y,Morita I

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Mon Nov 03 00:00:00 EST 2014

Dynamic and nuclear expression of PDGFRα and IGF-1R in alveolar Rhabdomyosarcoma.

Molecular cancer research : MCR

Aslam MI,Hettmer S,Abraham J,Latocha D,Soundararajan A,Huang ET,Goros MW,Michalek JE,Wang S,Mansoor A,Druker BJ,Wagers AJ,Tyner JW,Keller C

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Totipotent embryonic stem cells arise in ground-state culture conditions.

Cell reports

Morgani SM,Canham MA,Nichols J,Sharov AA,Migueles RP,Ko MS,Brickman JM

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Thu Jun 27 00:00:00 EDT 2013

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

ER71 directs mesodermal fate decisions during embryogenesis.

Development (Cambridge, England)

Rasmussen TL,Kweon J,Diekmann MA,Belema-Bedada F,Song Q,Bowlin K,Shi X,Ferdous A,Li T,Kyba M,Metzger JM,Koyano-Nakagawa N,Garry DJ

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2011

CCAAT/enhancer binding protein beta regulates stem cell activity and specifies luminal cell fate in the mammary gland.

Stem cells (Dayton, Ohio)

LaMarca HL,Visbal AP,Creighton CJ,Liu H,Zhang Y,Behbod F,Rosen JM

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Wed Mar 31 00:00:00 EDT 2010

Bioluminescence imaging of Olig2-neural stem cells reveals improved engraftment in a demyelination mouse model.

Stem cells (Dayton, Ohio)

Sher F,van Dam G,Boddeke E,Copray S

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 25-1401-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2009

Olig2+ neuroepithelial motoneuron progenitors are not multipotent stem cells in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Mukouyama YS,Deneen B,Lukaszewicz A,Novitch BG,Wichterle H,Jessell TM,Anderson DJ

Tue Jan 31 00:00:00 EST 2006

Tlx, an orphan nuclear receptor, regulates cell numbers and astrocyte development in the developing retina.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Miyawaki T,Uemura A,Dezawa M,Yu RT,Ide C,Nishikawa S,Honda Y,Tanabe Y,Tanabe T

Wed Sep 15 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF alpha chain; PDGF Receptor alpha; PDGF-R-alpha; pdgfr-a; PDGFR-alpha; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor alpha

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Gene Aliases: AI115593; CD140a; Pdgfr-2; Pdgfra

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UniProt ID: (Mouse) P26618

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Entrez Gene ID: (Mouse) 18595

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity platelet-derived growth factor alpha-receptor activity vascular endothelial growth factor-activated receptor activity platelet-derived growth factor receptor binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding vascular endothelial growth factor binding protein homodimerization activity phosphatidylinositol 3-kinase binding platelet-derived growth factor binding transmembrane signal receptor

Process(es)

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CD140a (PDGFRA) Monoclonal Antibody (APA5), PE-Cyanine7, eBioscience™

CD140a (PDGFRA) Antibody (25-1401-82) in Flow

Product Details

25-1401-82

Immunohistochemistry (Frozen) (IHC (F))

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