Invitrogen

CD144 (VE-cadherin) Monoclonal Antibody (eBioBV13 (BV13)), eFluor™ 660, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

44 Published Figures

32 References

View all (56) VE-cadherin (CD144) antibodies

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Protocols

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Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)

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CD144 (VE-cadherin) Antibody (50-1441-82) in Flow

Staining of bEnd-3 cells with 0.5 µg of Rat IgG1 K Isotype Control eFluor® 660 (Product # 50-4301-82) (blue histogram) or 0.5 µg of Anti-Mouse CD144 (VE-Cadherin) eFluor® 660 (Alexa Fluor® 647 Replacement) (purple histogram). Total viable cells were used for analysis.

CD144 (VE-cadherin) Antibody in Immunocytochemistry (ICC/IF)

CD144 (VE-cadherin) Antibody in Immunohistochemistry (IHC)

CD144 (VE-cadherin) Antibody in Western Blot (WB)

CD144 (VE-cadherin) Antibody in Flow Cytometry, ChIP Assay, Peptide array (Flow, ChIP, ARRAY)

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Product Details

50-1441-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

View 8 publications 8 publications

Flow Cytometry (Flow)

1 µg/test

View 14 publications 14 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Peptide Array (Array)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioBV13 (BV13)

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11219483

Product Specific Information

Description: The eBioBV13 (BV13) monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells.

Applications Reported: This eBioBV13 (BV13) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioBV13 (BV13) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Flow pattern-dependent mitochondrial dynamics regulates the metabolic profile and inflammatory state of endothelial cells.

JCI insight

Hong SG,Shin J,Choi SY,Powers JC,Meister BM,Sayoc J,Son JS,Tierney R,Recchia FA,Brown MD,Yang X,Park JY

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Immunohistochemistry

Thu Sep 22 00:00:00 EDT 2022

Adiponectin receptors sustain haematopoietic stem cells throughout adulthood by protecting them from inflammation.

Nature cell biology

Meacham CE,Jeffery EC,Burgess RJ,Sivakumar CD,Arora MA,Stanley AM,Colby EM,Crane GM,Zhao Z,Morrison SJ

50-1441-82 was used in Flow cytometry/Cell sorting to find that adiponectin receptors are non-cell-autonomously required in haematopoietic cells to promote HSC quiescence and self-renewal.

Sun May 01 00:00:00 EDT 2022

Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?

Aging cell

Zhang Z,Na H,Gan Q,Tao Q,Alekseyev Y,Hu J,Yan Z,Yang JB,Tian H,Zhu S,Li Q,Rajab IM,Blusztajn JK,Wolozin B,Emili A,Zhang X,Stein T,Potempa LA,Qiu WQ

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Western Blot

Mon Nov 01 00:00:00 EDT 2021

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Western Blot

Tue May 11 00:00:00 EDT 2021

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Western Blot

Tue May 11 00:00:00 EDT 2021

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Western Blot

Tue May 11 00:00:00 EDT 2021

The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition.

Cell reports

Azzoni E,Frontera V,Anselmi G,Rode C,James C,Deltcheva EM,Demian AS,Brown J,Barone C,Patelli A,Harman JR,Nicholls M,Conway SJ,Morrissey E,Jacobsen SEW,Sparrow DB,Harris AL,Enver T,de Bruijn MFTR

50-1441-82 was used in Immunocytochemistry-immunoflourescence to investigate how and when the onset of circulation affects Hematopoietic stem cells emergence.

Tue Dec 14 00:00:00 EST 2021

Local Mitochondrial ATP Production Regulates Endothelial Fatty Acid Uptake and Transport.

Cell metabolism

Ibrahim A,Yucel N,Kim B,Arany Z

50-1441-82 was used in Flow Cytometry to elucidate the mechanisms behind endothelial fatty acid uptake and transport.

Tue Aug 04 00:00:00 EDT 2020

VE-cadherin endocytosis controls vascular integrity and patterning during development.

The Journal of cell biology

Grimsley-Myers CM,Isaacson RH,Cadwell CM,Campos J,Hernandes MS,Myers KR,Seo T,Giang W,Griendling KK,Kowalczyk AP

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Immunocytochemistry

Mon May 04 00:00:00 EDT 2020

VE-cadherin endocytosis controls vascular integrity and patterning during development.

The Journal of cell biology

Grimsley-Myers CM,Isaacson RH,Cadwell CM,Campos J,Hernandes MS,Myers KR,Seo T,Giang W,Griendling KK,Kowalczyk AP

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Immunocytochemistry

Mon May 04 00:00:00 EDT 2020

Interleukin 35 Delays Hindlimb Ischemia-Induced Angiogenesis Through Regulating ROS-Extracellular Matrix but Spares Later Regenerative Angiogenesis.

Frontiers in immunology

Fu H,Sun Y,Shao Y,Saredy J,Cueto R,Liu L,Drummer C,Johnson C,Xu K,Lu Y,Li X,Meng S,Xue ER,Tan J,Jhala NC,Yu D,Zhou Y,Bayless KJ,Yu J,Rogers TJ,Hu W,Snyder NW,Sun J,Qin X,Jiang X,Wang H,Yang X

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Flow Cytometry

Fri Jul 09 00:00:00 EDT 2021

Endothelial CDS2 deficiency causes VEGFA-mediated vascular regression and tumor inhibition.

Cell research

Zhao W,Cao L,Ying H,Zhang W,Li D,Zhu X,Xue W,Wu S,Cao M,Fu C,Qi H,Hao Y,Tang YC,Qin J,Zhong TP,Lin X,Yu L,Li X,Li L,Wu D,Pan W

50-1441-82 was used in Flow Cytometry to indicate that the effect of VEGFA on vasculature is context-dependent and can be converted from angiogenesis to vascular regression.

Fri Nov 01 00:00:00 EDT 2019

Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor Targeting.

Cancer cell

Srivastava S,Salter AI,Liggitt D,Yechan-Gunja S,Sarvothama M,Cooper K,Smythe KS,Dudakov JA,Pierce RH,Rader C,Riddell SR

50-1441 was used in Flow cytometry/Cell sorting to demonstrate the use of “AND” logic gates as a strategy to increase the portfolio of cell surface molecules on solid tumors that can be targeted safely with CAR-T cells.

Mon Mar 18 00:00:00 EDT 2019

Metabolic Imaging Reveals a Unique Preference of Symmetric Cell Division and Homing of Leukemia-Initiating Cells in an Endosteal Niche.

Cell metabolism

Hao X,Gu H,Chen C,Huang D,Zhao Y,Xie L,Zou Y,Shu HS,Zhang Y,He X,Lai X,Zhang X,Zhou BO,Zhang CC,Chen GQ,Yu Z,Yang Y,Zheng J

50-1441 was used in Immunocytochemistry-immunoflourescence to provide a unique angle for the study of metabolisms in stem cells, and may lead to development of novel strategies for cancer treatment.

Tue Apr 02 00:00:00 EDT 2019

Cellular cartography of the organ of Corti based on optical tissue clearing and machine learning.

eLife

Urata S,Iida T,Yamamoto M,Mizushima Y,Fujimoto C,Matsumoto Y,Yamasoba T,Okabe S

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Immunohistochemistry

Fri Jan 18 00:00:00 EST 2019

Iron deficiency disrupts embryonic haematopoiesis but not the endothelial to haematopoietic transition.

Scientific reports

Shvartsman M,Bilican S,Lancrin C

50-1441 was used in Flow cytometry/Cell sorting to explore how cellular iron status affects embryonic haematopoiesis.

Tue Apr 23 00:00:00 EDT 2019

CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature.

Nature neuroscience

Louveau A,Herz J,Alme MN,Salvador AF,Dong MQ,Viar KE,Herod SG,Knopp J,Setliff JC,Lupi AL,Da Mesquita S,Frost EL,Gaultier A,Harris TH,Cao R,Hu S,Lukens JR,Smirnov I,Overall CC,Oliver G,Kipnis J

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Immunocytochemistry

Mon Oct 01 00:00:00 EDT 2018

Role of Notch1 in the arterial specification and angiogenic potential of mouse embryonic stem cell-derived endothelial cells.

Stem cell research & therapy

Park JK,Lee TW,Do EK,Moon HJ,Kim JH

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Flow Cytometry

Wed Jul 18 00:00:00 EDT 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in ChIP assay

Tue Mar 20 00:00:00 EDT 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in ChIP assay

Tue Mar 20 00:00:00 EDT 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in ChIP assay

Tue Mar 20 00:00:00 EDT 2018

Tumour ischaemia by interferon-γ resembles physiological blood vessel regression.

Nature

Kammertoens T,Friese C,Arina A,Idel C,Briesemeister D,Rothe M,Ivanov A,Szymborska A,Patone G,Kunz S,Sommermeyer D,Engels B,Leisegang M,Textor A,Fehling HJ,Fruttiger M,Lohoff M,Herrmann A,Yu H,Weichselbaum R,Uckert W,Hübner N,Gerhardt H,Beule D,Schreiber H,Blankenstein T

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Immunocytochemistry

Thu May 04 00:00:00 EDT 2017

A molecular roadmap of the AGM region reveals BMPER as a novel regulator of HSC maturation.

The Journal of experimental medicine

McGarvey AC,Rybtsov S,Souilhol C,Tamagno S,Rice R,Hills D,Godwin D,Rice D,Tomlinson SR,Medvinsky A

50-1441 was used in Flow cytometry/Cell sorting to identify novel secreted regulators of haematopoietic stem cell maturation.

Mon Dec 04 00:00:00 EST 2017

Activation of the TGFβ pathway impairs endothelial to haematopoietic transition.

Scientific reports

Vargel Ö,Zhang Y,Kosim K,Ganter K,Foehr S,Mardenborough Y,Shvartsman M,Enright AJ,Krijgsveld J,Lancrin C

50-1441 was used in Flow cytometry/Cell sorting to assess the contribution of transforming growth factor-beta 1 signalling in key haematological developmental processes during embryogenesis.

Fri Feb 19 00:00:00 EST 2016

Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development.

eLife

Ramo K,Sugamura K,Craige S,Keaney JF,Davis RJ

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Western Blot

Tue Aug 09 00:00:00 EDT 2016

EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta.

Scientific reports

Chen II,Caprioli A,Ohnuki H,Kwak H,Porcher C,Tosato G

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Flow Cytometry

Thu Jun 02 00:00:00 EDT 2016

Angiopoietin 2 regulates the transformation and integrity of lymphatic endothelial cell junctions.

Genes & development

Zheng W,Nurmi H,Appak S,Sabine A,Bovay E,Korhonen EA,Orsenigo F,Lohela M,D'Amico G,Holopainen T,Leow CC,Dejana E,Petrova TV,Augustin HG,Alitalo K

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Immunofluorescence

Tue Jul 15 00:00:00 EDT 2014

Sox7 is regulated by ETV2 during cardiovascular development.

Stem cells and development

Behrens AN,Zierold C,Shi X,Ren Y,Koyano-Nakagawa N,Garry DJ,Martin CM

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

The expression of Sox17 identifies and regulates haemogenic endothelium.

Nature cell biology

Clarke RL,Yzaguirre AD,Yashiro-Ohtani Y,Bondue A,Blanpain C,Pear WS,Speck NA,Keller G

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

HIF-1α activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells.

Cell communication and signaling : CCS

Weidemann A,Breyer J,Rehm M,Eckardt KU,Daniel C,Cicha I,Giehl K,Goppelt-Struebe M

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Immunofluorescence

Mon Oct 21 00:00:00 EDT 2013

Discontinuous LYVE-1 expression in corneal limbal lymphatics: dual function as microvalves and immunological hot spots.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Nakao S,Zandi S,Faez S,Kohno R,Hafezi-Moghadam A

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Immunofluorescence

Wed Feb 01 00:00:00 EST 2012

Endothelial-myofibroblast transition contributes to the early development of diabetic renal interstitial fibrosis in streptozotocin-induced diabetic mice.

The American journal of pathology

Li J,Qu X,Bertram JF

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 50-1441-82) in Immunofluorescence

Thu Oct 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: 7B4/cadherin-5; Cadherin-5; CD144; Vascular endothelial cadherin; VE-cadherin

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Gene Aliases: 7B4; AA408225; Cd144; Cdh5; VE-Cad; Vec; VEcad; VECD

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UniProt ID: (Mouse) P55284

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Entrez Gene ID: (Mouse) 12562

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Function(s)

receptor binding calcium ion binding protein binding beta-catenin binding protein phosphatase binding ion channel binding metal ion binding cadherin

Process(es)

blood vessel maturation cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules negative regulation of cell proliferation single organismal cell-cell adhesion regulation of establishment of cell polarity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD144 (VE-cadherin) Monoclonal Antibody (eBioBV13 (BV13)), eFluor™ 660, eBioscience™

CD144 (VE-cadherin) Antibody (50-1441-82) in Flow

Product Details

50-1441-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Peptide Array (Array)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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