Invitrogen

CD150 Monoclonal Antibody (mShad150), APC, eBioscience™

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Antibody Testing Data (1)
Published Figures (5)

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CD150 Antibody (17-1502-80) in Flow

Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (left) or 0.06 µg of Anti-Mouse CD150 APC (right). Total viable cells were used for analysis.

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Product Details

17-1502-80

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, lambda

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

mShad150

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1582223

Product Specific Information

Description: The mShad150 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T cells, in particular Th1, and B cells; this expression is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. The mShad150 antibody has been reported to also stain the CD150+CD244-CD48-CD41- population of pluripotent hematopoietic stem cells.

Applications Reported: This mShad150 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This mShad150 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells. Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses.

Wellcome open research

Wu G,Murugesan G,Nagala M,McCraw A,Haslam SM,Dell A,Crocker PR

Published figure using CD150 monoclonal antibody (Product # 17-1502-80) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS.

Journal of neuroinflammation

Hohsfield LA,Najafi AR,Ghorbanian Y,Soni N,Hingco EE,Kim SJ,Jue AD,Swarup V,Inlay MA,Green KN

Published figure using CD150 monoclonal antibody (Product # 17-1502-80) in Flow Cytometry

Sun Sep 20 00:00:00 EDT 2020

Id1 and Id3 Maintain Steady-State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration.

Cell reports

Gadomski S,Singh SK,Singh S,Sarkar T,Klarmann KD,Berenschot M,Seaman S,Jakubison B,Gudmundsson KO,Lockett S,Keller JR

17-1502 was used in Flow cytometry/Cell sorting to investigate the effects of loss of ld genes on adult endothelial cell.

Tue Apr 28 00:00:00 EDT 2020

Recipient bone marrow assimilates the myeloid/lymphoid reconstitution of distinct fetal hematopoietic stem cells.

Oncotarget

Guo XL,Chu L,Ke F,Mu LL,Li Z,Cai JJ,Li HF,Hong DL

Published figure using CD150 monoclonal antibody (Product # 17-1502-80) in Flow Cytometry

Tue Dec 12 00:00:00 EST 2017

Small molecule p300/catenin antagonist enhances hematopoietic recovery after radiation.

PloS one

Zhao Y,Wu K,Nguyen C,Smbatyan G,Melendez E,Higuchi Y,Chen Y,Kahn M

Published figure using CD150 monoclonal antibody (Product # 17-1502-80) in Flow Cytometry

Tue Sep 26 00:00:00 EDT 2017

Aging-associated inflammation promotes selection for adaptive oncogenic events in B cell progenitors.

The Journal of clinical investigation

Henry CJ,Casás-Selves M,Kim J,Zaberezhnyy V,Aghili L,Daniel AE,Jimenez L,Azam T,McNamee EN,Clambey ET,Klawitter J,Serkova NJ,Tan AC,Dinarello CA,DeGregori J

17-1502 was used in Flow cytometry/Cell sorting to conclude that chronic inflammatory microenvironments in old age lead to reductions in the fitness of B cell progenitor populations.

Tue Dec 01 00:00:00 EST 2015

Smurf2 regulates hematopoietic stem cell self-renewal and aging.

Aging cell

Ramkumar C,Kong Y,Trabucco SE,Gerstein RM,Zhang H

17-1502 was used in Flow cytometry/Cell sorting to suggest that Smurf2 plays an important role in regulating HSC self-renewal and ageing.

Sun Jun 01 00:00:00 EDT 2014

Superior long-term repopulating capacity of G-CSF+plerixafor-mobilized blood: implications for stem cell gene therapy by studies in the Hbb(th-3) mouse model.

Human gene therapy methods

Psatha N,Sgouramali E,Gkountis A,Siametis A,Baliakas P,Constantinou V,Athanasiou E,Arsenakis M,Anagnostopoulos A,Papayannopoulou T,Stamatoyannopoulos G,Yannaki E

17-1502 was used in Flow cytometry/Cell sorting to suggest that the G-CSF + plerixaformobilized cells represent a superior graft source for thalassemia gene therapy.

Mon Dec 01 00:00:00 EST 2014

Bcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo.

PloS one

Wu X,Satpathy AT,Kc W,Liu P,Murphy TL,Murphy KM

17-1502 was used in Flow cytometry/Cell sorting to investigate the role of Bcl11a in the development of dendritic cells, showing that it controls Flt3 expression in early hematopoietic progenitors and is required for pDC development.

Fri Jan 10 00:00:00 EST 2014

Zbtb46 expression distinguishes classical dendritic cells and their committed progenitors from other immune lineages.

The Journal of experimental medicine

Satpathy AT,KC W,Albring JC,Edelson BT,Kretzer NM,Bhattacharya D,Murphy TL,Murphy KM

17-1502 was used in Flow cytometry/Cell sorting to identify Zbtb46 as a transcription factor selectively expressed by classical DCs and their committed progenitors.

Mon Jun 04 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD150; CD150 antigen; signaling lymphocyte activation molecule; Signaling lymphocytic activation molecule; SLAM family member 1

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Gene Aliases: 4933415F16; AA177906; CD150; CDw150; ESTM51; IPO-3; Slam; Slamf1

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UniProt ID: (Mouse) Q9QUM4

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Entrez Gene ID: (Mouse) 27218

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Function(s)

receptor activity protein binding immunoglobulin receptor superfamily

Process(es)

regulation of vesicle fusion lymphocyte activation regulation of catalytic activity

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CD150 Monoclonal Antibody (mShad150), APC, eBioscience™

CD150 Antibody (17-1502-80) in Flow

Product Details

17-1502-80

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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