Invitrogen

CD16/CD32 Monoclonal Antibody (93), PE, eBioscience™

7 Published Figures

35 References

View all (45) CD16/CD32 antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (7)

CD16/CD32 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

CD16/CD32 Antibody (12-0161-82) in Flow

Staining of C57BL/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of Anti-Mouse CD16/CD32 PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-0161-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 34 publications 34 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Fruit fly, Mouse, Rat

Host/Isotype

Rat / IgG2a, lambda

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465568

Product Specific Information

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis.

Veterinary research

Feng L,Luo G,Li Y,Zhang C,Liu Y,Liu Y,Chen H,He D,Zhu Y,Gan L

12-0161-82 was used in Flow cytometry/Cell sorting to demonstrate that CUR protected against PRV-induced viral encephalitis by switching the phenotype of BV2 cells, thereby protecting neurons from inflammatory injury, and this effect was mediated by improving mitochondrial function and the AMPK/NF-κB p65-energy metabolism-related pathway.

Tue Mar 14 00:00:00 EDT 2023

Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy.

Nature medicine

Ganan-Gomez I,Yang H,Ma F,Montalban-Bravo G,Thongon N,Marchica V,Richard-Carpentier G,Chien K,Manyam G,Wang F,Alfonso A,Chen S,Class C,Kanagal-Shamanna R,Ingram JP,Ogoti Y,Rose A,Loghavi S,Lockyer P,Cambo B,Muftuoglu M,Schneider S,Adema V,McLellan M,Garza J,Marchesini M,Giuliani N,Pellegrini M,Wang J,Walker J,Li Z,Takahashi K,Leverson JD,Bueso-Ramos C,Andreeff M,Clise-Dwyer K,Garcia-Manero G,Colla S

12-0161-82 was used in Flow Cytometry to study the biological mechanisms that drive hypomethylating agent therapy failure at the stem-cell level to uncover vulnerabilities in the disease and halt its evolution.

Tue Mar 01 00:00:00 EST 2022

CD47 restricts antiviral function of alveolar macrophages during influenza virus infection.

iScience

Wenzek C,Steinbach P,Wirsdörfer F,Sutter K,Boehme JD,Geffers R,Klopfleisch R,Bruder D,Jendrossek V,Buer J,Westendorf AM,Knuschke T

12-0161-82 was used in Ex vivo AEC II infection assayto show a negative role for CD47 during antiviral immune responses in the lung by confining HB expression in aMФ.

Thu Dec 22 00:00:00 EST 2022

Nonredundancy of IL-1α and IL-1β is defined by distinct regulation of tissues orchestrating resistance versus tolerance to infection.

Science advances

Eislmayr K,Bestehorn A,Morelli L,Borroni M,Vande Walle L,Lamkanfi M,Kovarik P

12-0161-82 was used in Flow Cytometry to investigate why interleukin-1α and -1β are not redundant in vivo despite triggering the same receptor and eliciting comparable cellular responses.

Fri Mar 04 00:00:00 EST 2022

Nonredundancy of IL-1α and IL-1β is defined by distinct regulation of tissues orchestrating resistance versus tolerance to infection.

Science advances

Eislmayr K,Bestehorn A,Morelli L,Borroni M,Vande Walle L,Lamkanfi M,Kovarik P

12-0161-82 was used in Flow Cytometry to investigate why interleukin-1α and -1β are not redundant in vivo despite triggering the same receptor and eliciting comparable cellular responses.

Fri Mar 04 00:00:00 EST 2022

Cytosolic Nuclear Sensor Dhx9 Controls Medullary Thymic Epithelial Cell Differentiation by p53-Mediated Pathways.

Frontiers in immunology

Dong X,Zhang J,Zhang Q,Liang Z,Xu Y,Zhao Y,Zhang B

12-0161-82 was used in Flow cytometry/Cell sorting to reveal that Dhx9, a cytosolic nuclear sensor recognizes viral DNA or RNA, playing an important role in mTEC development and function in mice.

Tue Jun 21 00:00:00 EDT 2022

TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity.

Cell stem cell

Muto T,Guillamot M,Yeung J,Fang J,Bennett J,Nadorp B,Lasry A,Redondo LZ,Choi K,Gong Y,Walker CS,Hueneman K,Bolanos LC,Barreyro L,Lee LH,Greis KD,Vasyliev N,Khodadadi-Jamayran A,Nudler E,Lujambio A,Lowe SW,Aifantis I,Starczynowski DT

12-0161-82 was used in Flow cytometry/Cell sorting to identify signaling states that cooperate with pre-leukemic cells and, notably, the study aimed to uncover the role of the ubiquitin ligase TRAF6 in preventing the transition from a pre-leukemic stage to overt myeloid leukemia by regulating MYC through ubiquitination and acetylation mechanisms.

Thu Feb 03 00:00:00 EST 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 12-0161-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Genistein Attenuates Isoflurane-Induced Neuroinflammation by Inhibiting TLR4-Mediated Microglial-Polarization in vivo and in vitro.

Journal of inflammation research

Jiang T,Xu S,Shen Y,Xu Y,Li Y

12-0161-82 was used in Flow Cytometry to studied the protective effects of genistein on isoflurane-induced neuroinflammation in rats and BV2 cells.

Sun Apr 24 00:00:00 EDT 2022

C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor.

Cancer cell

Di Genua C,Valletta S,Buono M,Stoilova B,Sweeney C,Rodriguez-Meira A,Grover A,Drissen R,Meng Y,Beveridge R,Aboukhalil Z,Karamitros D,Belderbos ME,Bystrykh L,Thongjuea S,Vyas P,Nerlov C

Published figure using CD16/CD32 monoclonal antibody (Product # 12-0161-82) in Flow Cytometry

Mon May 11 00:00:00 EDT 2020

Asymmetrically Segregated Mitochondria Provide Cellular Memory of Hematopoietic Stem Cell Replicative History and Drive HSC Attrition.

Cell stem cell

Hinge A,He J,Bartram J,Javier J,Xu J,Fjellman E,Sesaki H,Li T,Yu J,Wunderlich M,Mulloy J,Kofron M,Salomonis N,Grimes HL,Filippi MD

12-0161 was used in Flow cytometry/Cell sorting to investigate the underlying role of mitochondria in hematopoietic stem cells (HSCs) to meet change of metabolic requirements during their cell cycle activity.

Thu Mar 05 00:00:00 EST 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 12-0161-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Potently Cytotoxic Natural Killer Cells Initially Emerge from Erythro-Myeloid Progenitors during Mammalian Development.

Developmental cell

Dege C,Fegan KH,Creamer JP,Berrien-Elliott MM,Luff SA,Kim D,Wagner JA,Kingsley PD,McGrath KE,Fehniger TA,Palis J,Sturgeon CM

12-0161 was used in Flow cytometry/Cell sorting to identify an extra-embryonic origin of potently cytotoxic NK cells, suggesting that ontogenic origin is a relevant factor in designing hPSC-derived adoptive immunotherapies.

Mon Apr 20 00:00:00 EDT 2020

Use of Busulfan to Condition Mice for Bone Marrow Transplantation.

STAR protocols

Montecino-Rodriguez E,Dorshkind K

12-0161 was used in Flow cytometry/Cell sorting to describe a protocol for the use of busulfan to condition mice for bone marrow transplantation and several of the variables to consider for effective implementation.

Fri Dec 18 00:00:00 EST 2020

β4GALT1 controls β1 integrin function to govern thrombopoiesis and hematopoietic stem cell homeostasis.

Nature communications

Giannini S,Lee-Sundlov MM,Rivadeneyra L,Di Buduo CA,Burns R,Lau JT,Falet H,Balduini A,Hoffmeister KM

12-0161 was used in Flow cytometry/Cell sorting to identify the conserved B4galt1 gene as a critical regulator of thrombopoiesis in megakaryocytes.

Fri Jan 17 00:00:00 EST 2020

Single-nucleotide human disease mutation inactivates a blood-regenerative GATA2 enhancer.

The Journal of clinical investigation

Soukup AA,Zheng Y,Mehta C,Wu J,Liu P,Cao M,Hofmann I,Zhou Y,Zhang J,Johnson KD,Choi K,Keles S,Bresnick EH

12-0161 was used in Flow cytometry/Cell sorting to study the mechanisms underlying how GATA2 enhancer disease mutations impact hematopoiesis and pathology.

Fri Mar 01 00:00:00 EST 2019

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

Published figure using CD16/CD32 monoclonal antibody (Product # 12-0161-82) in Flow Cytometry

Wed Jun 20 00:00:00 EDT 2018

Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.

Nature

Hérault A,Binnewies M,Leong S,Calero-Nieto FJ,Zhang SY,Kang YA,Wang X,Pietras EM,Chu SH,Barry-Holson K,Armstrong S,Göttgens B,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 12-0161-82) in Flow Cytometry

Thu Apr 06 00:00:00 EDT 2017

GATA Factor-Regulated Samd14 Enhancer Confers Red Blood Cell Regeneration and Survival in Severe Anemia.

Developmental cell

Hewitt KJ,Katsumura KR,Matson DR,Devadas P,Tanimura N,Hebert AS,Coon JJ,Kim JS,Dewey CN,Keles S,Hao S,Paulson RF,Bresnick EH

12-0161 was used in Flow cytometry/Cell sorting to propose that Samd14-Enh and an ensemble of anemia-responsive enhancers are essential for erythrocyte regeneration in stress erythropoiesis.

Mon Aug 07 00:00:00 EDT 2017

Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.

Nature communications

Liu Z,Ravindranathan R,Kalinski P,Guo ZS,Bartlett DL

Published figure using CD16/CD32 monoclonal antibody (Product # 12-0161-82) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2017

PML-RARA requires DNA methyltransferase 3A to initiate acute promyelocytic leukemia.

The Journal of clinical investigation

Cole CB,Verdoni AM,Ketkar S,Leight ER,Russler-Germain DA,Lamprecht TL,Demeter RT,Magrini V,Ley TJ

12-0161 was used in Flow cytometry/Cell sorting to suggest that PML-RARA requires DNMT3A to initiate acute promyelocytic leukemia in mice.

Fri Jan 01 00:00:00 EST 2016

Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis.

mBio

Schlam D,Canton J,Carreño M,Kopinski H,Freeman SA,Grinstein S,Fairn GD

Published figure using CD16/CD32 monoclonal antibody (Product # 12-0161-82) in Flow Cytometry

Tue Apr 05 00:00:00 EDT 2016

Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma.

Journal for immunotherapy of cancer

Patel MA,Kim JE,Theodros D,Tam A,Velarde E,Kochel CM,Francica B,Nirschl TR,Ghasemzadeh A,Mathios D,Harris-Bookman S,Jackson CC,Jackson C,Ye X,Tran PT,Tyler B,Coric V,Selby M,Brem H,Drake CG,Pardoll DM,Lim M

Published figure using CD16/CD32 monoclonal antibody (Product # 12-0161-82) in Flow Cytometry

Wed May 18 00:00:00 EDT 2016

Angiotensin converting enzyme inhibitors and angiotensin II receptor antagonist attenuate tumor growth via polarization of neutrophils toward an antitumor phenotype.

Oncoimmunology

Shrestha S,Noh JM,Kim SY,Ham HY,Kim YJ,Yun YJ,Kim MJ,Kwon MS,Song DK,Hong CW

12-0161 was used in Flow cytometry/Cell sorting to investigate how modulation of Ang II pathway attenuates tumour growth via polarisation of neutrophils to an antitumoural phenotype.

Tue Jan 12 00:00:00 EST 2021

EphA2 Is a Therapy Target in EphA2-Positive Leukemias but Is Not Essential for Normal Hematopoiesis or Leukemia.

PloS one

Charmsaz S,Beckett K,Smith FM,Bruedigam C,Moore AS,Al-Ejeh F,Lane SW,Boyd AW

12-0161 was used in Flow cytometry/Cell sorting to explore the role of EphA2 in haematopoiesis and leukaemogenesis.

Tue May 03 00:00:00 EDT 2016

Central role of conventional dendritic cells in regulation of bone marrow release and survival of neutrophils.

Journal of immunology (Baltimore, Md. : 1950)

Jiao J,Dragomir AC,Kocabayoglu P,Rahman AH,Chow A,Hashimoto D,Leboeuf M,Kraus T,Moran T,Carrasco-Avino G,Friedman SL,Merad M,Aloman C

12-0161 was used in Flow cytometry/Cell sorting to investigate the role of dendritic cells in neutrophil homeostasis, showing a central role in regulation of bone marrow release and survival of neutrophils.

Tue Apr 01 00:00:00 EDT 2014

Distinct development and functions of resident and recruited liver Kupffer cells/macrophages.

Journal of leukocyte biology

Ikarashi M,Nakashima H,Kinoshita M,Sato A,Nakashima M,Miyazaki H,Nishiyama K,Yamamoto J,Seki S

12-0161 was used in Flow cytometry/Cell sorting to study how liver CD68+ Kupffer cells and CD11b+ Kupffer cells/macrophages are developmentally and functionally distinct subsets.

Sun Dec 01 00:00:00 EST 2013

Targeting the shift from M1 to M2 macrophages in experimental autoimmune encephalomyelitis mice treated with fasudil.

PloS one

Liu C,Li Y,Yu J,Feng L,Hou S,Liu Y,Guo M,Xie Y,Meng J,Zhang H,Xiao B,Ma C

12-0161 was used in Flow cytometry/Cell sorting to investigate the effect and mechanism of Fasudil on experimental autoimmune encephalomyelitis.

Fri Aug 09 00:00:00 EDT 2013

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Published figure using CD16/CD32 monoclonal antibody (Product # 12-0161-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Published figure using CD16/CD32 monoclonal antibody (Product # 12-0161-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD16/CD32 monoclonal antibody (Product # 12-0161-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

The autophagy protein Atg7 is essential for hematopoietic stem cell maintenance.

The Journal of experimental medicine

Mortensen M,Soilleux EJ,Djordjevic G,Tripp R,Lutteropp M,Sadighi-Akha E,Stranks AJ,Glanville J,Knight S,Jacobsen SE,Kranc KR,Simon AK

12-0161 was used in Flow cytometry/Cell sorting to show that Atg7 is an essential regulator of adult hematopoietic stem cell maintenance.

Mon Mar 14 00:00:00 EDT 2011

Functional characterization of high levels of meningioma 1 as collaborating oncogene in acute leukemia.

Leukemia

Liu T,Jankovic D,Brault L,Ehret S,Baty F,Stavropoulou V,Rossi V,Biondi A,Schwaller J

12-0161 was used in Flow cytometry/Cell sorting to investigate the role of MN1 in leukemia, showing that it is a collaborating oncogene in acute leukemia.

Mon Mar 01 00:00:00 EST 2010

Influence of 5 major Salmonella pathogenicity islands on NK cell depletion in mice infected with Salmonella enterica serovar Enteritidis.

BMC microbiology

Karasova D,Sebkova A,Havlickova H,Sisak F,Volf J,Faldyna M,Ondrackova P,Kummer V,Rychlik I

12-0161 was used in Flow cytometry/Cell sorting to investigate the colonisation and early immune response of Balb/C mice to infection with Salmonella Enteritidis and isogenic pathogenicity island free mutants.

Fri Mar 12 00:00:00 EST 2010

Gene expression profiling of leukemia stem cells.

Methods in molecular biology (Clifton, N.J.)

Krivtsov AV,Wang Y,Feng Z,Armstrong SA

12-0161 was used in Flow cytometry/Cell sorting to illustrate methods to isolate a leukemia stem cell population, amplify RNA, and perform microarray analyses.

Tue May 19 00:00:00 EDT 2009

Identification of regulatory functions for 4-1BB and 4-1BBL in myelopoiesis and the development of dendritic cells.

Nature immunology

Lee SW,Park Y,So T,Kwon BS,Cheroutre H,Mittler RS,Croft M

12-0161 was used in Flow cytometry/Cell sorting to show that the costimulatory molecule 4-1BB, and also its ligand 4-1BBL, is able to limit myelopoiesis and dendritic cell development.

Fri Aug 01 00:00:00 EDT 2008

Characterization of fibroblasts responsible for cartilage destruction in arthritis.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Treese C,Mittag A,Lange F,Tarnok A,Loesche A,Emmrich F,Lehmann J,Sack U

12-0161 was used in Flow cytometry/Cell sorting to show that LS48 is an excellent tool to gain a deeper understanding of synovial fibroblasts in rheumatoid arthritis.

Tue Apr 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD16A; CD16B; CD32; CD32 receptor 2; Fc gamma receptor IIB; Fc gamma receptor III; Fc gamma RIIB; Fc-gamma RII; fc-gamma RIII; Fc-gamma-RII; fc-gamma-RIIB; Fcg receptor III; FCG2; FcgammaRIIB; FcgammaRIII; FCGR2C; FCGR3A; FCGR3B; FCGRIII; FCR-10; FcRII-b; FcRII-c; fcRIII; FCRIIIA; FGFR2B; IgG Fc receptor II beta; igG Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; low affinity immunoglobulin gamma Fc region receptor III; Ly-17; Lymphocyte antigen 17; RP11-5K23.1

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Gene Aliases: AI528646; CD16; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

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UniProt ID: (Mouse) P08101

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Entrez Gene ID: (Mouse) 14130, (Mouse) 14131

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Function(s)

receptor signaling protein activity protein binding IgG binding immunoglobulin binding IgG receptor activity immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

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CD16/CD32 Monoclonal Antibody (93), PE, eBioscience™

CD16/CD32 Antibody (12-0161-82) in Flow

Product Details

12-0161-82

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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