Invitrogen

CD16/CD32 Monoclonal Antibody (93), PerCP-Cyanine5.5, eBioscience™

7 Published Figures

31 References

View all (45) CD16/CD32 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (7)

CD16/CD32 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

CD16/CD32 Antibody (45-0161-82) in Flow

Staining of BALB/c splenocytes with 0.06 µg of Rat IgG2a K Isotype Control PerCP-Cyanine5-5 (Product # 45-4321-80) (open histogram) or 0.06 µg of Anti-Mouse CD16/CD32 PerCP-Cyanine5-5 (filled histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

45-0161-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 31 publications 31 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fruit fly, Mouse

Host/Isotype

Rat / IgG2a, lambda

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_996659

Product Specific Information

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported: This 93 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 93 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Nonredundancy of IL-1α and IL-1β is defined by distinct regulation of tissues orchestrating resistance versus tolerance to infection.

Science advances

Eislmayr K,Bestehorn A,Morelli L,Borroni M,Vande Walle L,Lamkanfi M,Kovarik P

Published figure using CD16/CD32 monoclonal antibody (Product # 45-0161-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Mapping and role of T cell response in SARS-CoV-2-infected mice.

The Journal of experimental medicine

Zhuang Z,Lai X,Sun J,Chen Z,Zhang Z,Dai J,Liu D,Li Y,Li F,Wang Y,Zhu A,Wang J,Yang W,Huang J,Li X,Hu L,Wen L,Zhuo J,Zhang Y,Chen D,Li S,Huang S,Shi Y,Zheng K,Zhong N,Zhao J,Zhou D,Zhao J

45-0161 was used in Flow cytometry/Cell sorting to demonstrate cross-reactive T cell responses between SARS-CoV and SARS-CoV-2, but not MERS-CoV, in mice.

Mon Apr 05 00:00:00 EDT 2021

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 45-0161-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Generation of a Broadly Useful Model for COVID-19 Pathogenesis, Vaccination, and Treatment.

Cell

Sun J,Zhuang Z,Zheng J,Li K,Wong RL,Liu D,Huang J,He J,Zhu A,Zhao J,Li X,Xi Y,Chen R,Alshukairi AN,Chen Z,Zhang Z,Chen C,Huang X,Li F,Lai X,Chen D,Wen L,Zhuo J,Zhang Y,Wang Y,Huang S,Dai J,Shi Y,Zheng K,Leidinger MR,Chen J,Li Y,Zhong N,Meyerholz DK,McCray PB,Perlman S,Zhao J

45-0161 was used in Flow cytometry/Cell sorting to describe a murine model of immediate utility to investigate COVID-19 pathogenesis and new therapies to treat it.

Thu Aug 06 00:00:00 EDT 2020

C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor.

Cancer cell

Di Genua C,Valletta S,Buono M,Stoilova B,Sweeney C,Rodriguez-Meira A,Grover A,Drissen R,Meng Y,Beveridge R,Aboukhalil Z,Karamitros D,Belderbos ME,Bystrykh L,Thongjuea S,Vyas P,Nerlov C

Published figure using CD16/CD32 monoclonal antibody (Product # 45-0161-82) in Flow Cytometry

Mon May 11 00:00:00 EDT 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 45-0161-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

tagHi-C Reveals 3D Chromatin Architecture Dynamics during Mouse Hematopoiesis.

Cell reports

Zhang C,Xu Z,Yang S,Sun G,Jia L,Zheng Z,Gu Q,Tao W,Cheng T,Li C,Cheng H

45-0161-82 was used in Flow Cytometry to introduce a low-input tagmentation-based Hi-C (tagHi-C) method to capture the chromatin structures of hundreds of cells.

Tue Sep 29 00:00:00 EDT 2020

Androgen Receptor Signaling Positively Regulates Monocytic Development.

Frontiers in immunology

Consiglio CR,Gollnick SO

45-0161-82 was used in Flow Cytometry to investigate the role of androgen signaling in monocytic differentiation with a genetic mouse model of myeloid androgen receptor (AR) knockout (MARKO) and pharmacological inhibition of AR.

Thu Apr 29 00:00:00 EDT 2021

LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis.

Nature

Peltzer N,Darding M,Montinaro A,Draber P,Draberova H,Kupka S,Rieser E,Fisher A,Hutchinson C,Taraborrelli L,Hartwig T,Lafont E,Haas TL,Shimizu Y,Böiers C,Sarr A,Rickard J,Alvarez-Diaz S,Ashworth MT,Beal A,Enver T,Bertin J,Kaiser W,Strasser A,Silke J,Bouillet P,Walczak H

45-0161-82 was used in Flow cytometry/Cell sorting to demonstrate that both linear ubiquitin chain assembly complex components HOIP and HOIL-1, are required for embryogenesis by preventing cell death.

Tue May 01 00:00:00 EDT 2018

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

Published figure using CD16/CD32 monoclonal antibody (Product # 45-0161-82) in Flow Cytometry

Wed Jun 20 00:00:00 EDT 2018

The creatine kinase pathway is a metabolic vulnerability in EVI1-positive acute myeloid leukemia.

Nature medicine

Fenouille N,Bassil CF,Ben-Sahra I,Benajiba L,Alexe G,Ramos A,Pikman Y,Conway AS,Burgess MR,Li Q,Luciano F,Auberger P,Galinsky I,DeAngelo DJ,Stone RM,Zhang Y,Perkins AS,Shannon K,Hemann MT,Puissant A,Stegmaier K

45-0161 was used in Flow cytometry/Cell sorting to investigate the effect of MECOM on cellular metabolism.

Wed Mar 01 00:00:00 EST 2017

Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.

Nature

Hérault A,Binnewies M,Leong S,Calero-Nieto FJ,Zhang SY,Kang YA,Wang X,Pietras EM,Chu SH,Barry-Holson K,Armstrong S,Göttgens B,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 45-0161-82) in Flow Cytometry

Thu Apr 06 00:00:00 EDT 2017

Ubiquitination of hnRNPA1 by TRAF6 links chronic innate immune signaling with myelodysplasia.

Nature immunology

Fang J,Bolanos LC,Choi K,Liu X,Christie S,Akunuru S,Kumar R,Wang D,Chen X,Greis KD,Stoilov P,Filippi MD,Maciejewski JP,Garcia-Manero G,Weirauch MT,Salomonis N,Geiger H,Zheng Y,Starczynowski DT

45-0161 was used in Flow cytometry/Cell sorting to examine the significance of ubiquitin signalling in coordinating RNA processing by Toll-like receptor pathways during an immune response and in premalignant haematologic diseases.

Wed Feb 01 00:00:00 EST 2017

Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.

Nature communications

Liu Z,Ravindranathan R,Kalinski P,Guo ZS,Bartlett DL

Published figure using CD16/CD32 monoclonal antibody (Product # 45-0161-82) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2017

CD36 receptor regulates malaria-induced immune responses primarily at early blood stage infection contributing to parasitemia control and resistance to mortality.

The Journal of biological chemistry

Thylur RP,Wu X,Gowda NM,Punnath K,Neelgund SE,Febbraio M,Gowda DC

45-0161 was used in Flow cytometry/Cell sorting to study the function of CD36 in malaria immunity.

Fri Jun 02 00:00:00 EDT 2017

Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis.

mBio

Schlam D,Canton J,Carreño M,Kopinski H,Freeman SA,Grinstein S,Fairn GD

Published figure using CD16/CD32 monoclonal antibody (Product # 45-0161-82) in Flow Cytometry

Tue Apr 05 00:00:00 EDT 2016

Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma.

Journal for immunotherapy of cancer

Patel MA,Kim JE,Theodros D,Tam A,Velarde E,Kochel CM,Francica B,Nirschl TR,Ghasemzadeh A,Mathios D,Harris-Bookman S,Jackson CC,Jackson C,Ye X,Tran PT,Tyler B,Coric V,Selby M,Brem H,Drake CG,Pardoll DM,Lim M

Published figure using CD16/CD32 monoclonal antibody (Product # 45-0161-82) in Flow Cytometry

Wed May 18 00:00:00 EDT 2016

Endothelial Cell-Selective Adhesion Molecule Expression in Hematopoietic Stem/Progenitor Cells Is Essential for Erythropoiesis Recovery after Bone Marrow Injury.

PloS one

Sudo T,Yokota T,Okuzaki D,Ueda T,Ichii M,Ishibashi T,Isono T,Habuchi Y,Oritani K,Kanakura Y

45-0161 was used in Flow cytometry/Cell sorting to determine the physiological importance of Endothelial Cell-Selective Adhesion Molecule (ESAM) in erythroid recovery, showing that ESAM is essential for erthryopoiesis recovery following bone marrow injury.

Tue Mar 14 00:00:00 EDT 2017

The GIY-YIG Type Endonuclease Ankyrin Repeat and LEM Domain-Containing Protein 1 (ANKLE1) Is Dispensable for Mouse Hematopoiesis.

PloS one

Braun J,Meixner A,Brachner A,Foisner R

45-0161 was used in Flow cytometry/Cell sorting to test whether ANKLE1 may contribute to Holliday junction resolution during DNA repair in mice.

Fri Aug 12 00:00:00 EDT 2016

Targeting megakaryocytic-induced fibrosis in myeloproliferative neoplasms by AURKA inhibition.

Nature medicine

Wen QJ,Yang Q,Goldenson B,Malinge S,Lasho T,Schneider RK,Breyfogle LJ,Schultz R,Gilles L,Koppikar P,Abdel-Wahab O,Pardanani A,Stein B,Gurbuxani S,Mullally A,Levine RL,Tefferi A,Crispino JD

45-0161 was used in Flow cytometry/Cell sorting to demonstrate that megakaryocytes drive fibrosis in PMF and that targeting them with AURKA inhibitors has the potential for therapeutic benefit.

Tue Dec 01 00:00:00 EST 2015

Telomerase Deficiency Causes Alveolar Stem Cell Senescence-associated Low-grade Inflammation in Lungs.

The Journal of biological chemistry

Chen R,Zhang K,Chen H,Zhao X,Wang J,Li L,Cong Y,Ju Z,Xu D,Williams BR,Jia J,Liu JP

45-0161 was used in Flow cytometry/Cell sorting to elucidate the mechanisms by which TERC and TERT participate in lung diseases.

Fri Dec 25 00:00:00 EST 2015

The miR-126-VEGFR2 axis controls the innate response to pathogen-associated nucleic acids.

Nature immunology

Agudo J,Ruzo A,Tung N,Salmon H,Leboeuf M,Hashimoto D,Becker C,Garrett-Sinha LA,Baccarini A,Merad M,Brown BD

45-0161 was used in Flow cytometry/Cell sorting to identify the miR-126-VEGFR2 axis as an important regulator of the innate response that operates through multiscale control of pDCs.

Wed Jan 01 00:00:00 EST 2014

Diet-induced obesity promotes myelopoiesis in hematopoietic stem cells.

Molecular metabolism

Singer K,DelProposto J,Morris DL,Zamarron B,Mergian T,Maley N,Cho KW,Geletka L,Subbaiah P,Muir L,Martinez-Santibanez G,Lumeng CN

45-0161 was used in Flow cytometry/Cell sorting to demonstrate how metabolic signals potentiate leukocyte production and dietary priming of haematopoietic progenitors contributes to adipose tissue inflammation.

Mon Sep 01 00:00:00 EDT 2014

Wnt5a regulates hematopoietic stem cell proliferation and repopulation through the Ryk receptor.

Stem cells (Dayton, Ohio)

Povinelli BJ,Nemeth MJ

45-0161 was used in Flow cytometry/Cell sorting to study whether Ryk protein regulates the response of haematopoietic stem cells to Wnt5a.

Wed Jan 01 00:00:00 EST 2014

Notch2-dependent classical dendritic cells orchestrate intestinal immunity to attaching-and-effacing bacterial pathogens.

Nature immunology

Satpathy AT,Briseño CG,Lee JS,Ng D,Manieri NA,Kc W,Wu X,Thomas SR,Lee WL,Turkoz M,McDonald KG,Meredith MM,Song C,Guidos CJ,Newberry RD,Ouyang W,Murphy TL,Stappenbeck TS,Gommerman JL,Nussenzweig MC,Colonna M,Kopan R,Murphy KM

45-0161 was used in Flow cytometry/Cell sorting to elucidate the cellular source of essential IL-23 production required for the mucosal response to attaching-and-effacing bacterial infection.

Sun Sep 01 00:00:00 EDT 2013

Bcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo.

PloS one

Wu X,Satpathy AT,Kc W,Liu P,Murphy TL,Murphy KM

45-0161 was used in Flow cytometry/Cell sorting to investigate the role of Bcl11a in the development of dendritic cells, showing that it controls Flt3 expression in early hematopoietic progenitors and is required for pDC development.

Fri Jan 10 00:00:00 EST 2014

Zbtb46 expression distinguishes classical dendritic cells and their committed progenitors from other immune lineages.

The Journal of experimental medicine

Satpathy AT,KC W,Albring JC,Edelson BT,Kretzer NM,Bhattacharya D,Murphy TL,Murphy KM

45-0161 was used in Flow cytometry/Cell sorting to identify Zbtb46 as a transcription factor selectively expressed by classical DCs and their committed progenitors.

Mon Jun 04 00:00:00 EDT 2012

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Published figure using CD16/CD32 monoclonal antibody (Product # 45-0161-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Published figure using CD16/CD32 monoclonal antibody (Product # 45-0161-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD16/CD32 monoclonal antibody (Product # 45-0161-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

IFN-γ- and IL-10-expressing virus epitope-specific Foxp3(+) T reg cells in the central nervous system during encephalomyelitis.

The Journal of experimental medicine

Zhao J,Zhao J,Fett C,Trandem K,Fleming E,Perlman S

45-0161 was used in Flow cytometry/Cell sorting to show that pathogen epitope-specific Foxp3(+) T reg cells can be identified on the basis of cytokine production.

Mon Aug 01 00:00:00 EDT 2011

Regulation of granulocyte and macrophage populations of murine bone marrow cells by G-CSF and CD137 protein.

PloS one

Jiang D,Schwarz H

45-0161 was used in Flow cytometry/Cell sorting to confirm the role of CD137 ligand signalling in regulating myelopoiesis, and demonstrate that this growth promoting influence is restricted to the monocytic lineage.

Mon Dec 13 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CD16A; CD16B; CD32; CD32 receptor 2; Fc gamma receptor IIB; Fc gamma receptor III; Fc gamma RIIB; Fc-gamma RII; fc-gamma RIII; Fc-gamma-RII; fc-gamma-RIIB; Fcg receptor III; FCG2; FcgammaRIIB; FcgammaRIII; FCGR2C; FCGR3A; FCGR3B; FCGRIII; FCR-10; FcRII-b; FcRII-c; fcRIII; FCRIIIA; FGFR2B; IgG Fc receptor II beta; igG Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; low affinity immunoglobulin gamma Fc region receptor III; Ly-17; Lymphocyte antigen 17; RP11-5K23.1

View more View less

Gene Aliases: AI528646; CD16; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

View more View less

UniProt ID: (Mouse) P08101

View more View less

Entrez Gene ID: (Mouse) 14130, (Mouse) 14131

View more View less

Function(s)

receptor signaling protein activity protein binding IgG binding immunoglobulin binding IgG receptor activity immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD16/CD32 Monoclonal Antibody (93), PerCP-Cyanine5.5, eBioscience™

CD16/CD32 Antibody (45-0161-82) in Flow

Product Details

45-0161-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!