Invitrogen

CD178 (Fas Ligand) Monoclonal Antibody (MFL3), PE, eBioscience™

CD178 (Fas Ligand) Antibody in Flow Cytometry (Flow)

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CD178 (Fas Ligand) Antibody (12-5911-82) in Flow

Staining of mouse CD178-transfected cells with 0.5 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (blue histogram) or 0.5 µg of Anti-Mouse CD178 (Fas Ligand) PE (purple histogram). Total viable cells were used for analysis.

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Product Details

12-5911-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MFL3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466039

Product Specific Information

Description: The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by mouse activated T cells. The interaction of FasL with its receptor CD95 induces Fas-mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. The degree of sensitivity for the mouse antigen to MMPs has not been reported.

Applications Reported: The MFL3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The MFL3 antibody has been tested by flow cytometric analysis of mouse FasL transfected cells and activated T cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD178 (Fas ligand, FasL) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor, FasR, is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. As a member of the TNF-cytokine family CD178 induces apoptosis when interacting with FasR. CD178 may exist as either membrane bound (45 kD) or soluble forms (26 kD). The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of CD178 to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins. CD178 is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. CD178 expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney. It is assumed that induction of apoptosis through CD178 is predominantly involved in anti-viral immune responses. CD178 is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express FAS, where CD178 is expressed predominantly on activated T cells. FAS and CD178 are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity. CD178 concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension. The Fas/ CD178 system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 12-5911-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

A macrophage-T cell coculture model for severe tissue injury-induced T cell death.

STAR protocols

Zhu J,Cao J,Liesz A,Roth S

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 12-5911-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Silencing of soluble epoxide hydrolase 2 gene reduces H₂O₂-induced oxidative damage in rat intestinal epithelial IEC-6 cells via activating PI3K/Akt/GSK3β signaling pathway.

Cytotechnology

Li J,Luo J,Zhang Y,Tang C,Wang J,Chen C

12-5911 was used in Western Blotting to explore the effects and molecular mechanism of siEPHX2 on H2O2-induced oxidative damage in rat intestinal epithelial IEC-6 cells.

Sat Feb 01 00:00:00 EST 2020

CD4⁺ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium Development.

Frontiers in immunology

Cobbold SP,Adams E,Howie D,Waldmann H

Mon Jan 31 00:00:00 EST 2022

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 12-5911-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Hypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury.

Journal of the American Society of Nephrology : JASN

Zhang J,Han C,Dai H,Hou J,Dong Y,Cui X,Xu L,Zhang M,Xia Q

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 12-5911-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells.

PLoS pathogens

Frenkel D,Zhang F,Guirnalda P,Haynes C,Bockstal V,Radwanska M,Magez S,Black SJ

12-5911 was used in Flow cytometry/Cell sorting to investigate the mechanism by which natural killer cells in Trypanosoma brucei infected mice kill B cells, suppress humoral immunity and induce early mortality.

Fri Jul 01 00:00:00 EDT 2016

Activated cytotoxic lymphocytes promote tumor progression by increasing the ability of 3LL tumor cells to mediate MDSC chemoattraction via Fas signaling.

Cellular & molecular immunology

Yang F,Wei Y,Cai Z,Yu L,Jiang L,Zhang C,Yan H,Wang Q,Cao X,Liang T,Wang J

12-5911 was used in Flow cytometry/Cell sorting to highlight a novel mechanism whereby cytotoxic T lymphocytes may participate in the tumour immune evasion process.

Thu Jan 01 00:00:00 EST 2015

The NF-κB regulator Bcl-3 governs dendritic cell antigen presentation functions in adaptive immunity.

Journal of immunology (Baltimore, Md. : 1950)

Tassi I,Claudio E,Wang H,Tang W,Ha HL,Saret S,Ramaswamy M,Siegel R,Siebenlist U

12-5911 was used in Flow cytometry/Cell sorting to study the role of Bcl-3 in dendritic cell survival and the generation of adaptive immunity.

Sat Nov 01 00:00:00 EDT 2014

Inhibition of eIF5A results in aberrant uterine natural killer cell function and embryo loss in mice.

American journal of reproductive immunology (New York, N.Y. : 1989)

Qin X,Liu X,Shan B,Shi L,Sharma S,Wu J,Lin Y

12-5911 was used in Flow cytometry/Cell sorting to investigate the effects of N1-guanyl-1,7-diaminoheptane, an inhibitor of eukaryotic initiation factor 5A, on natural killer cells in vitro.

Sat Mar 01 00:00:00 EST 2014

Cancer immunotherapy employing an innovative strategy to enhance CD4+ T cell help in the tumor microenvironment.

PloS one

Song L,Yang MC,Knoff J,Wu TC,Hung CF

12-5911 was used in Flow cytometry/Cell sorting to investigate the effect of intratumoral injection of PADRE and the adjuvant CpG into HPV16 E7-expressing TC-1 tumours following cisplatin chemotherapy, showing potent antitumor effects and antigen-specific cell-mediated immune response.

Tue Dec 15 00:00:00 EST 2015

Enhanced effector responses in activated CD8+ T cells deficient in diacylglycerol kinases.

Cancer research

Riese MJ,Wang LC,Moon EK,Joshi RP,Ranganathan A,June CH,Koretzky GA,Albelda SM

12-5911 was used in Flow cytometry/Cell sorting to investigate whether activation of T cells using chimeric antigen receptor can be enhanced by deletion of negative regulators, showing that deletion of dgk enhanced cytokine production and were effective at limiting growth of implanted tumours.

Sat Jun 15 00:00:00 EDT 2013

Induction of Anti-Tumor Immune Responses by Peptide Receptor Radionuclide Therapy with (177)Lu-DOTATATE in a Murine Model of a Human Neuroendocrine Tumor.

Diagnostics (Basel, Switzerland)

Wu Y,Pfeifer AK,Myschetzky R,Garbyal RS,Rasmussen P,Knigge U,Bzorek M,Kristensen MH,Kjaer A

12-5911 was used in Flow cytometry/Cell sorting to study anti-tumor immune response induction by peptide receptor radionuclide therapy.

Wed Oct 02 00:00:00 EDT 2013

Increased killing of liver NK cells by Fas/Fas ligand and NKG2D/NKG2D ligand contributes to hepatocyte necrosis in virus-induced liver failure.

Journal of immunology (Baltimore, Md. : 1950)

Zou Y,Chen T,Han M,Wang H,Yan W,Song G,Wu Z,Wang X,Zhu C,Luo X,Ning Q

12-5911 was used in Flow cytometry/Cell sorting to reveal a pivotal role for liver natural killer cells in the pathogenesis of virus-induced fulminant hepatic failure and acute-on-chronic liver failure.

Fri Jan 01 00:00:00 EST 2010

Effect of polyI:C cotreatment on halothane-induced liver injury in mice.

Hepatology (Baltimore, Md.)

Cheng L,You Q,Yin H,Holt M,Franklin C,Ju C

12-5911 was used in Flow cytometry/Cell sorting to investigate the effect of viral infection on halothane-induced hepatotoxicity in mice using a viral RNA mimetic.

Thu Jan 01 00:00:00 EST 2009

RNA interference-mediated in vivo silencing of fas ligand as a strategy for the enhancement of DNA vaccine potency.

Human gene therapy

Huang B,Mao CP,Peng S,Hung CF,Wu TC

12-5911 was used in Immunofluorescence to indicate that coadministration of DNA encoding shRNA targeting luciferase significantly reduces luciferase expression in mice intradermally administered luciferase DNA.

Fri Aug 01 00:00:00 EDT 2008

Rapid default transition of CD4 T cell effectors to functional memory cells.

The Journal of experimental medicine

McKinstry KK,Golech S,Lee WH,Huston G,Weng NP,Swain SL

12-5911 was used in Flow cytometry/Cell sorting to follow well-defined cohorts of CD4 T cells through the effector-to-memory transition by analyzing phenotype, important functional properties, and gene expression profiles.

Mon Sep 03 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: Apoptosis (APO 1) antigen ligand 1; CD178; CD178 antigen; CD95 ligand; CD95 ligand protein;Generalized lymphoproliferative disease (Gld); CD95-L; Fas antigen ligand; Fas ligand; Fas-LG; generalized lymphoproliferative disease; soluble form; TNFL; Tumor necrosis factor (ligand) superfamily member 6; Tumor necrosis factor ligand; Tumor necrosis factor ligand superfamily member 6; Tumor necrosis factor ligand superfamily member 6 (TNFL6 / TNFSF6)

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Gene Aliases: APT1LG1; CD178; CD95-L; CD95L; Fas-L; Fasl; Faslg; gld; Tnfsf6

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UniProt ID: (Mouse) P41047

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Entrez Gene ID: (Mouse) 14103

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Function(s)

death receptor binding cytokine activity tumor necrosis factor receptor binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory cell apoptotic process immune response positive regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors negative regulation of angiogenesis cellular chloride ion homeostasis positive regulation of apoptotic process positive regulation of neuron apoptotic process positive regulation of epidermal growth factor receptor signaling pathway retinal cell programmed cell death endosomal lumen acidification T cell apoptotic process necroptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway necroptotic signaling pathway positive regulation of endothelial cell apoptotic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD178 (Fas Ligand) Monoclonal Antibody (MFL3), PE, eBioscience™

CD178 (Fas Ligand) Antibody (12-5911-82) in Flow

Product Details

12-5911-82

Western Blot (WB)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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