Invitrogen

CD184 (CXCR4) Monoclonal Antibody (12G5), PE, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

19 Published Figures

30 References

View all (76) CXCR4 (CD184) antibodies

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Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD184 (CXCR4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 22

CD184 (CXCR4) Antibody (12-9999-42) in Flow

Knockout of CXCR4 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR695599_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CXCR4 was performed by staining HeLa CXCR4 Knock out cells with 0.25 µg Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ (Product # 12-4724-82, yellow histogram) or 0.25 µg CD184 (CXCR4) Monoclonal Antibody (12G5), PE, eBioscience™ (Product # 12-9999-42, blue histogram). HeLa Cas9 control cells were also stained w... View More

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Product Details

12-9999-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 29 publications 29 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Sheep

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

12G5

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10669164

Product Specific Information

Description: The 12G5 monoclonal antibody reacts with human CD184 (CXCR4), also termed Fusin, LESTR, or HUMSTR. CD184 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. The 12G5 antibody shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CD184), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates.

Applications Reported: This 12G5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 12G5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Airway and Lung Organoids from Human-Induced Pluripotent Stem Cells Can Be Used to Assess CFTR Conductance.

International journal of molecular sciences

Demchenko A,Kondrateva E,Tabakov V,Efremova A,Salikhova D,Bukharova T,Goldshtein D,Balyasin M,Bulatenko N,Amelina E,Lavrov A,Smirnikhina S

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2023

Amanita muscaria extract potentiates production of proinflammatory cytokines by dsRNA-activated human microglia.

Frontiers in pharmacology

Wagner A,Pehar M,Yan Z,Kulka M

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Tue May 02 00:00:00 EDT 2023

Dynamics of CXCR4 positive circulating tumor cells in prostate cancer patients during radiotherapy.

International journal of cancer

Klusa D,Lohaus F,Franken A,Baumbach M,Cojoc M,Dowling P,Linge A,Offermann A,Löck S,Hušman D,Rivandi M,Polzer B,Freytag V,Lange T,Neubauer H,Kücken M,Perner S,Hölscher T,Dubrovska A,Krause M,Kurth I,Baumann M,Peitzsch C

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2023

Parallel functional assessment of m⁶A sites in human endodermal differentiation with base editor screens.

Nature communications

Cheng W,Liu F,Ren Z,Chen W,Chen Y,Liu T,Ma Y,Cao N,Wang J

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Tue Jan 25 00:00:00 EST 2022

Rapamycin Pretreatment Rescues the Bone Marrow AML Cell Elimination Capacity of CAR-T Cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Nian Z,Zheng X,Dou Y,Du X,Zhou L,Fu B,Sun R,Tian Z,Wei H

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Rapamycin Pretreatment Rescues the Bone Marrow AML Cell Elimination Capacity of CAR-T Cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Nian Z,Zheng X,Dou Y,Du X,Zhou L,Fu B,Sun R,Tian Z,Wei H

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Cotargeting of miR-126-3p and miR-221-3p inhibits PIK3R2 and PTEN, reducing lung cancer growth and metastasis by blocking AKT and CXCR4 signalling.

Molecular oncology

Di Paolo D,Pontis F,Moro M,Centonze G,Bertolini G,Milione M,Mensah M,Segale M,Petraroia I,Borzi C,Suatoni P,Brignole C,Perri P,Ponzoni M,Pastorino U,Sozzi G,Fortunato O

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphoma.

Nature communications

Zhang S,Jiang VC,Han G,Hao D,Lian J,Liu Y,Zhang R,McIntosh J,Wang R,Dang M,Dai E,Wang Y,Santos D,Badillo M,Leeming A,Chen Z,Hartig K,Bigcal J,Zhou J,Kanagal-Shamanna R,Ok CY,Lee H,Steiner RE,Zhang J,Song X,Nair R,Ahmed S,Rodriquez A,Thirumurthi S,Jain P,Wagner-Bartak N,Hill H,Nomie K,Flowers C,Futreal A,Wang L,Wang M

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Mon May 17 00:00:00 EDT 2021

Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

International journal of inflammation

Genkel V,Dolgushin I,Baturina I,Savochkina A,Kuznetsova A,Pykhova L,Shaposhnik I

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Sun Apr 24 00:00:00 EDT 2022

Enhanced CXCR4 Expression of Human CD8^Low T Lymphocytes Is Driven by S1P₄.

Frontiers in immunology

Burkard T,Dreis C,Herrero San Juan M,Huhn M,Weigert A,Pfeilschifter JM,Radeke HH

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.

Oncology letters

Zou Y,Liang J,Li D,Fang J,Wang L,Wang J,Zhang J,Guo Q,Yan X,Tang H

12-9999 was used in Flow cytometry/Cell sorting to demonstrate that modification of the CK-CKR axis using exogenous recombinant chemokines at the proper time point enhanced CIK cell trafficking ability and improved CIK antitumor effects.

Wed Jul 01 00:00:00 EDT 2020

Circulating Progenitor Cells in Patients With Coronary Artery Disease and Renal Insufficiency.

JACC. Basic to translational science

Mehta A,Tahhan AS,Liu C,Dhindsa DS,Nayak A,Hooda A,Moazzami K,Islam SJ,Rogers SC,Almuwaqqat Z,Mokhtari A,Hesaroieh I,Ko YA,Waller EK,Quyyumi AA

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

Combined single-cell profiling of expression and DNA methylation reveals splicing regulation and heterogeneity.

Genome biology

Linker SM,Urban L,Clark SJ,Chhatriwala M,Amatya S,McCarthy DJ,Ebersberger I,Vallier L,Reik W,Stegle O,Bonder MJ

12-9999 was used in Flow cytometry/Cell sorting to apply parallel DNA methylation and transcriptome sequencing to differentiating human induced pluripotent stem cells to characterize splicing variation (exon skipping) and its determinants.

Mon Feb 11 00:00:00 EST 2019

A library of ATTR amyloidosis patient-specific induced pluripotent stem cells for disease modelling and in vitro testing of novel therapeutics.

Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis

Giadone RM,Rosarda JD,Akepati PR,Thomas AC,Boldbaatar B,James MF,Wilson AA,Sanchorawala V,Connors LH,Berk JL,Wiseman RL,Murphy GJ

12-9999-42 was used in Flow Cytometry to characterize six representative iPSC lines from a library of previously reprogrammed iPSC lines and reprogrammable blood samples derived from ATTR amyloidosis patients.

Sat Sep 01 00:00:00 EDT 2018

Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells.

Molecular medicine reports

Ran LJ,Zeng Y,Wang SC,Zhang DS,Hong M,Li SY,Dong J,Shi MX

12-9999-42 was used in Flow Cytometry to investigate the effect of co‚culture with amniotic epithelial cells (AECs) on the biological characteristics of amniotic mesenchymal stem cells.

Sun Jul 01 00:00:00 EDT 2018

Modulatory effects of trophoblast-secreted CXCL12 on the migration and invasion of human first-trimester decidual epithelial cells are mediated by CXCR4 rather than CXCR7.

Reproductive biology and endocrinology : RB&E

Zheng J,Wang H,Zhou W

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Fri Mar 02 00:00:00 EST 2018

The CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signaling.

Cell communication and signaling : CCS

Berning P,Schaefer C,Clemens D,Korsching E,Dirksen U,Potratz J

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Fri May 18 00:00:00 EDT 2018

Improving the biological function of decellularized heart valves through integration of protein tethering and three-dimensional cell seeding in a bioreactor.

Journal of tissue engineering and regenerative medicine

Namiri M,Kazemi Ashtiani M,Abbasalizadeh S,Mazidi Z,Mahmoudi E,Nikeghbalian S,Aghdami N,Baharvand H

12-9999-42 was used in Flow Cytometry, Immunohistochemistry to show that integration of SDF-1α immobilization and 3D cell seeding in a bioreactor might provide a novel, promising approach for production of functional heart valves.

Sun Apr 01 00:00:00 EDT 2018

Improving the biological function of decellularized heart valves through integration of protein tethering and three-dimensional cell seeding in a bioreactor.

Journal of tissue engineering and regenerative medicine

Namiri M,Kazemi Ashtiani M,Abbasalizadeh S,Mazidi Z,Mahmoudi E,Nikeghbalian S,Aghdami N,Baharvand H

Sun Apr 01 00:00:00 EDT 2018

3D Microfluidic model for evaluating immunotherapy efficacy by tracking dendritic cell behaviour toward tumor cells.

Scientific reports

Parlato S,De Ninno A,Molfetta R,Toschi E,Salerno D,Mencattini A,Romagnoli G,Fragale A,Roccazzello L,Buoncervello M,Canini I,Bentivegna E,Falchi M,Bertani FR,Gerardino A,Martinelli E,Natale C,Paolini R,Businaro L,Gabriele L

12-9999 was used in Flow cytometry/Cell sorting to study the interactions between IFN-dendritic cells and cancer cells in response to anti-tumour agents.

Mon Apr 24 00:00:00 EDT 2017

ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Berger RP,Sun YH,Kulik M,Lee JK,Nairn AV,Moremen KW,Pierce M,Dalton S

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 12-9999-42) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells.

Methods in molecular biology (Clifton, N.J.)

Tian L,Prasad N,Jang YY

12-9999 was used in Flow cytometry/Cell sorting to study the effects of alcohol exposure on multistage hepatic cells to model the early stages of liver tissue injury associated with alcoholic liver disease.

Thu Aug 18 00:00:00 EDT 2016

In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells.

Methods in molecular biology (Clifton, N.J.)

Tian L,Prasad N,Jang YY

Thu Aug 18 00:00:00 EDT 2016

IL12-mediated sensitizing of T-cell receptor-dependent and -independent tumor cell killing.

Oncoimmunology

Braun M,Ress ML,Yoo YE,Scholz CJ,Eyrich M,Schlegel PG,Wölfl M

12-9999 was used in Flow cytometry/Cell sorting to investigate how IL12, and other inflammatory cytokines, shape effector functions of human T-cells.

Fri Jul 01 00:00:00 EDT 2016

A Periodic Diet that Mimics Fasting Promotes Multi-System Regeneration, Enhanced Cognitive Performance, and Healthspan.

Cell metabolism

Brandhorst S,Choi IY,Wei M,Cheng CW,Sedrakyan S,Navarrete G,Dubeau L,Yap LP,Park R,Vinciguerra M,Di Biase S,Mirzaei H,Mirisola MG,Childress P,Ji L,Groshen S,Penna F,Odetti P,Perin L,Conti PS,Ikeno Y,Kennedy BK,Cohen P,Morgan TE,Dorff TB,Longo VD

12-9999 was used in Flow cytometry/Cell sorting to study the effect of prolonged fasting on promoting multi-system regeneration, enhancing cognitive performance, and altering healthspan.

Tue Jul 07 00:00:00 EDT 2015

Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.

Infection and immunity

Miles B,Zakhary I,El-Awady A,Scisci E,Carrion J,O'Neill JC,Rawlings A,Stern JK,Susin C,Cutler CW

12-9999 was used in Flow cytometry/Cell sorting to investigate how the alteration in chemokine-chemokine receptor expression contributes to pathogen trafficking via myeloid dendritic cells, showing that P.gingivalis infection induces an angiogenic migratory profile and disrupts secondary lymphoid organ-homing CCR7.

Wed Jan 01 00:00:00 EST 2014

Infliximab induces downregulation of the IL-12/IL-23 axis in 6-sulfo-LacNac (slan)+ dendritic cells and macrophages.

The Journal of allergy and clinical immunology

Brunner PM,Koszik F,Reininger B,Kalb ML,Bauer W,Stingl G

12-9999 was used in Flow cytometry/Cell sorting to analyse the effects of anti-TNF-α treatment on TNF-α(+) cells in patients with psoriasis, showing that induces down-regulation of the IL-12/IL-23 axis.

Fri Nov 01 00:00:00 EDT 2013

Human cytomegalovirus infection inhibits CXCL12- mediated migration and invasion of human extravillous cytotrophoblasts.

Virology journal

Warner JA,Zwezdaryk KJ,Day B,Sullivan DE,Pridjian G,Morris CA

12-9999 was used in Flow cytometry/Cell sorting to investigate the effect of human cytomegalovirus infection on the CXCL12-mediated migration and invasion of the SGHPL-4 cell line.

Thu Nov 01 00:00:00 EDT 2012

Identification and metastatic potential of tumor-initiating cells in malignant rhabdoid tumor of the kidney.

Clinical cancer research : an official journal of the American Association for Cancer Research

Yanagisawa S,Kadouchi I,Yokomori K,Hirose M,Hakozaki M,Hojo H,Maeda K,Kobayashi E,Murakami T

12-9999 was used in Flow cytometry/Cell sorting to suggest that CD133(+) cells determine the metastatic fate of malignant rhabdoid tumour of kidney cells and that CD133(-) cells may play an auxiliary role in tumour progression.

Fri May 01 00:00:00 EDT 2009

Inhibitory effect of triptolide on lymph node metastasis in patients with non-Hodgkin lymphoma by regulating SDF-1/CXCR4 axis in vitro.

Acta pharmacologica Sinica

Zhang C,Cui GH,Liu F,Wu QL,Chen Y

12-9999 was used in Flow cytometry/Cell sorting to investigate the antiproliferative effect of triptolide on B-NHL cells, study its effect on lymph node metastasis in vitro in patients with non-Hodgkin's lymphoma, and explore the mechanism regulating SDF-1/CXCR4 axis.

Wed Nov 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine receptor; CXC; CXC motif chemokine; CXC-R4; FB22; Fusin; HM89; HUMSTR; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPYRL; SDF-1 receptor; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor

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Gene Aliases: CD184; CXCR4; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; WHIMS

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UniProt ID: (Human) P61073

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Entrez Gene ID: (Human) 7852

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Function(s)

virus receptor activity actin binding G-protein coupled receptor activity protein binding drug binding coreceptor activity C-X-C chemokine receptor activity cytokine binding ubiquitin protein ligase binding myosin light chain binding ubiquitin binding

Process(es)

activation of MAPK activity response to hypoxia neuron migration epithelial cell development dendritic cell chemotaxis apoptotic process inflammatory response G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration neuron recognition response to virus fusion of virus membrane with host plasma membrane calcium-mediated signaling telencephalon cell migration entry into host cell regulation of cell migration regulation of programmed cell death myelin maintenance positive regulation of oligodendrocyte differentiation regulation of chemotaxis regulation of calcium ion transport cardiac muscle contraction chemokine-mediated signaling pathway cellular response to cytokine stimulus

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CD184 (CXCR4) Monoclonal Antibody (12G5), PE, eBioscience™

CD184 (CXCR4) Antibody (12-9999-42) in Flow

Product Details

12-9999-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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