Invitrogen

CD184 (CXCR4) Monoclonal Antibody (12G5), PE-Cyanine5, eBioscience™

CD184 (CXCR4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 19

CD184 (CXCR4) Antibody (15-9999-42) in Flow

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD184 (CXCR4) PE-Cyanine5 (filled histogram).Cells in the lymphocyte gate were used for analysis.

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Product Details

15-9999-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 23 publications 23 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

12G5

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10670201

Product Specific Information

Description: The 12G5 monoclonal antibody reacts with human CD184 (CXCR4), also termed Fusin, LESTR, or HUMSTR. CD184 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. The 12G5 antibody shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CD184), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates.

Applications Reported: The 12G5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 12G5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Amanita muscaria extract potentiates production of proinflammatory cytokines by dsRNA-activated human microglia.

Frontiers in pharmacology

Wagner A,Pehar M,Yan Z,Kulka M

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Tue May 02 00:00:00 EDT 2023

Airway and Lung Organoids from Human-Induced Pluripotent Stem Cells Can Be Used to Assess CFTR Conductance.

International journal of molecular sciences

Demchenko A,Kondrateva E,Tabakov V,Efremova A,Salikhova D,Bukharova T,Goldshtein D,Balyasin M,Bulatenko N,Amelina E,Lavrov A,Smirnikhina S

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2023

Dynamics of CXCR4 positive circulating tumor cells in prostate cancer patients during radiotherapy.

International journal of cancer

Klusa D,Lohaus F,Franken A,Baumbach M,Cojoc M,Dowling P,Linge A,Offermann A,Löck S,Hušman D,Rivandi M,Polzer B,Freytag V,Lange T,Neubauer H,Kücken M,Perner S,Hölscher T,Dubrovska A,Krause M,Kurth I,Baumann M,Peitzsch C

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2023

Parallel functional assessment of m⁶A sites in human endodermal differentiation with base editor screens.

Nature communications

Cheng W,Liu F,Ren Z,Chen W,Chen Y,Liu T,Ma Y,Cao N,Wang J

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Tue Jan 25 00:00:00 EST 2022

Rapamycin Pretreatment Rescues the Bone Marrow AML Cell Elimination Capacity of CAR-T Cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Nian Z,Zheng X,Dou Y,Du X,Zhou L,Fu B,Sun R,Tian Z,Wei H

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Cotargeting of miR-126-3p and miR-221-3p inhibits PIK3R2 and PTEN, reducing lung cancer growth and metastasis by blocking AKT and CXCR4 signalling.

Molecular oncology

Di Paolo D,Pontis F,Moro M,Centonze G,Bertolini G,Milione M,Mensah M,Segale M,Petraroia I,Borzi C,Suatoni P,Brignole C,Perri P,Ponzoni M,Pastorino U,Sozzi G,Fortunato O

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

15-9999 was used in Flow cytometry/Cell sorting to suggest senescent tumor cells generate a cytokine barrier protecting nonsenescent tumor cells from immune attack and provide a new target for overcoming the immunotherapy resistance of CRC.

Mon Feb 01 00:00:00 EST 2021

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

Mon Feb 01 00:00:00 EST 2021

Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphoma.

Nature communications

Zhang S,Jiang VC,Han G,Hao D,Lian J,Liu Y,Zhang R,McIntosh J,Wang R,Dang M,Dai E,Wang Y,Santos D,Badillo M,Leeming A,Chen Z,Hartig K,Bigcal J,Zhou J,Kanagal-Shamanna R,Ok CY,Lee H,Steiner RE,Zhang J,Song X,Nair R,Ahmed S,Rodriquez A,Thirumurthi S,Jain P,Wagner-Bartak N,Hill H,Nomie K,Flowers C,Futreal A,Wang L,Wang M

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Mon May 17 00:00:00 EDT 2021

Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

International journal of inflammation

Genkel V,Dolgushin I,Baturina I,Savochkina A,Kuznetsova A,Pykhova L,Shaposhnik I

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Sun Apr 24 00:00:00 EDT 2022

Enhanced CXCR4 Expression of Human CD8^Low T Lymphocytes Is Driven by S1P₄.

Frontiers in immunology

Burkard T,Dreis C,Herrero San Juan M,Huhn M,Weigert A,Pfeilschifter JM,Radeke HH

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Circulating Progenitor Cells in Patients With Coronary Artery Disease and Renal Insufficiency.

JACC. Basic to translational science

Mehta A,Tahhan AS,Liu C,Dhindsa DS,Nayak A,Hooda A,Moazzami K,Islam SJ,Rogers SC,Almuwaqqat Z,Mokhtari A,Hesaroieh I,Ko YA,Waller EK,Quyyumi AA

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Differential transcriptome and development of human peripheral plasma cell subsets.

JCI insight

Garimalla S,Nguyen DC,Halliley JL,Tipton C,Rosenberg AF,Fucile CF,Saney CL,Kyu S,Kaminski D,Qian Y,Scheuermann RH,Gibson G,Sanz I,Lee FE

15-9999 was used in Flow cytometry/Cell sorting to study the heterogeneity of human antibody secreting cells after vaccination.

Thu May 02 00:00:00 EDT 2019

Modulatory effects of trophoblast-secreted CXCL12 on the migration and invasion of human first-trimester decidual epithelial cells are mediated by CXCR4 rather than CXCR7.

Reproductive biology and endocrinology : RB&E

Zheng J,Wang H,Zhou W

15-9999 was used in Flow cytometry/Cell sorting to explore whether human first-trimester trophoblast cells were capable of modulating the migration and invasion of human first-trimester decidual epithelial cells via CXCL12/CXCR4/CXCR7 signaling.

Fri Mar 02 00:00:00 EST 2018

Modulatory effects of trophoblast-secreted CXCL12 on the migration and invasion of human first-trimester decidual epithelial cells are mediated by CXCR4 rather than CXCR7.

Reproductive biology and endocrinology : RB&E

Zheng J,Wang H,Zhou W

Fri Mar 02 00:00:00 EST 2018

The CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signaling.

Cell communication and signaling : CCS

Berning P,Schaefer C,Clemens D,Korsching E,Dirksen U,Potratz J

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Fri May 18 00:00:00 EDT 2018

ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Berger RP,Sun YH,Kulik M,Lee JK,Nairn AV,Moremen KW,Pierce M,Dalton S

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells.

Methods in molecular biology (Clifton, N.J.)

Tian L,Prasad N,Jang YY

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 15-9999-42) in Flow Cytometry

Thu Aug 18 00:00:00 EDT 2016

Porphyromonas gingivalis evasion of autophagy and intracellular killing by human myeloid dendritic cells involves DC-SIGN-TLR2 crosstalk.

PLoS pathogens

El-Awady AR,Miles B,Scisci E,Kurago ZB,Palani CD,Arce RM,Waller JL,Genco CA,Slocum C,Manning M,Schoenlein PV,Cutler CW

15-9999 was used in Flow cytometry/Cell sorting to reveal that selective engagement of DC-SIGN by Mfa-1+P. gingivalis results in intracellular persistence in myeloid DCs.

Sun Feb 01 00:00:00 EST 2015

Intracellular trafficking and endocytosis of CXCR4 in fetal mesenchymal stem/stromal cells.

BMC cell biology

Pelekanos RA,Ting MJ,Sardesai VS,Ryan JM,Lim YC,Chan JK,Fisk NM

15-9999 was used in Flow cytometry/Cell sorting to investigate the intracellular localisation of CXCR4 in fetal bone marrow-derived MSC, and the role of intracellular trafficking in CXCR4 surface expression and function.

Fri May 16 00:00:00 EDT 2014

Human secondary lymphoid organs typically contain polyclonally-activated proliferating regulatory T cells.

Blood

Peters JH,Koenen HJ,Fasse E,Tijssen HJ,Ijzermans JN,Groenen PJ,Schaap NP,Kwekkeboom J,Joosten I

15-9999 was used in Flow cytometry/Cell sorting to suggest that human secondary lymphoid organs aid in the maintenance and regulation of regulatory T-cell function and homeostasis.

Thu Sep 26 00:00:00 EDT 2013

Elucidation of seventeen human peripheral blood B-cell subsets and quantification of the tetanus response using a density-based method for the automated identification of cell populations in multidimensional flow cytometry data.

Cytometry. Part B, Clinical cytometry

Qian Y,Wei C,Eun-Hyung Lee F,Campbell J,Halliley J,Lee JA,Cai J,Kong YM,Sadat E,Thomson E,Dunn P,Seegmiller AC,Karandikar NJ,Tipton CM,Mosmann T,Sanz I,Scheuermann RH

15-9999 was used in Flow cytometry/Cell sorting to develop a novel program, FLOCK, to algorithmically identify biologically relevant cell populations from multiple samples.

Thu Dec 23 00:00:00 EST 2010

Human CD25highFoxp3pos regulatory T cells differentiate into IL-17-producing cells.

Blood

Koenen HJ,Smeets RL,Vink PM,van Rijssen E,Boots AM,Joosten I

15-9999 was used in Flow cytometry/Cell sorting to report that regulatory T cells can differentiate into IL-17-producing cells, when stimulated by antigen-presenting cells in the presence of rhIL-2/rhIL-15.

Mon Sep 15 00:00:00 EDT 2008

AMD3100 mobilizes hematopoietic stem cells with long-term repopulating capacity in nonhuman primates.

Blood

Larochelle A,Krouse A,Metzger M,Orlic D,Donahue RE,Fricker S,Bridger G,Dunbar CE,Hematti P

15-9999 was used in Flow cytometry/Cell sorting to study how AMD3100-mobilized CD34(+) cells represent an alternative source of hematopoietic stem cells for clinical stem cell transplantation and genetic manipulation with integrating retroviral vectors.

Mon May 01 00:00:00 EDT 2006

Flt3 ligand and the Flt3 receptor regulate hematopoietic cell migration by modulating the SDF-1alpha(CXCL12)/CXCR4 axis.

Blood

Fukuda S,Broxmeyer HE,Pelus LM

15-9999 was used in Flow cytometry/Cell sorting to investigate if Flt3 ligand and its receptor functionally interact with CXCL12/CXCR4 to regulate haematopoietic cell migration.

Fri Apr 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine receptor; CXC; CXC motif chemokine; CXC-R4; FB22; Fusin; HM89; HUMSTR; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPYRL; SDF-1 receptor; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor

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Gene Aliases: CD184; CXCR4; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; WHIMS

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UniProt ID: (Human) P61073

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Entrez Gene ID: (Human) 7852

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Function(s)

virus receptor activity actin binding G-protein coupled receptor activity protein binding drug binding coreceptor activity C-X-C chemokine receptor activity cytokine binding ubiquitin protein ligase binding myosin light chain binding ubiquitin binding

Process(es)

activation of MAPK activity response to hypoxia neuron migration epithelial cell development dendritic cell chemotaxis apoptotic process inflammatory response G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration neuron recognition response to virus fusion of virus membrane with host plasma membrane calcium-mediated signaling telencephalon cell migration entry into host cell regulation of cell migration regulation of programmed cell death myelin maintenance positive regulation of oligodendrocyte differentiation regulation of chemotaxis regulation of calcium ion transport cardiac muscle contraction chemokine-mediated signaling pathway cellular response to cytokine stimulus

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CD184 (CXCR4) Monoclonal Antibody (12G5), PE-Cyanine5, eBioscience™

CD184 (CXCR4) Antibody (15-9999-42) in Flow

Product Details

15-9999-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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