Invitrogen

CD184 (CXCR4) Monoclonal Antibody (12G5), PE-Cyanine7, eBioscience™

CD184 (CXCR4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 21

CD184 (CXCR4) Antibody (25-9999-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (Product # 11-0199-42) and Mouse IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4724-81) (left) or Anti-Human CD184 (CXCR4) PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

25-9999-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

12G5

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1659706

Product Specific Information

Description: The 12G5 monoclonal antibody reacts with human CD184 (CXCR4), also termed Fusin, LESTR, or HUMSTR. CD184 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. The 12G5 antibody shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CD184), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates.

Applications Reported: This 12G5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 12G5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Dynamics of CXCR4 positive circulating tumor cells in prostate cancer patients during radiotherapy.

International journal of cancer

Klusa D,Lohaus F,Franken A,Baumbach M,Cojoc M,Dowling P,Linge A,Offermann A,Löck S,Hušman D,Rivandi M,Polzer B,Freytag V,Lange T,Neubauer H,Kücken M,Perner S,Hölscher T,Dubrovska A,Krause M,Kurth I,Baumann M,Peitzsch C

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 25-9999-42) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2023

Amanita muscaria extract potentiates production of proinflammatory cytokines by dsRNA-activated human microglia.

Frontiers in pharmacology

Wagner A,Pehar M,Yan Z,Kulka M

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 25-9999-42) in Flow Cytometry

Tue May 02 00:00:00 EDT 2023

Airway and Lung Organoids from Human-Induced Pluripotent Stem Cells Can Be Used to Assess CFTR Conductance.

International journal of molecular sciences

Demchenko A,Kondrateva E,Tabakov V,Efremova A,Salikhova D,Bukharova T,Goldshtein D,Balyasin M,Bulatenko N,Amelina E,Lavrov A,Smirnikhina S

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 25-9999-42) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2023

Parallel functional assessment of m⁶A sites in human endodermal differentiation with base editor screens.

Nature communications

Cheng W,Liu F,Ren Z,Chen W,Chen Y,Liu T,Ma Y,Cao N,Wang J

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 25-9999-42) in Flow Cytometry

Tue Jan 25 00:00:00 EST 2022

Rapamycin Pretreatment Rescues the Bone Marrow AML Cell Elimination Capacity of CAR-T Cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Nian Z,Zheng X,Dou Y,Du X,Zhou L,Fu B,Sun R,Tian Z,Wei H

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 25-9999-42) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Cotargeting of miR-126-3p and miR-221-3p inhibits PIK3R2 and PTEN, reducing lung cancer growth and metastasis by blocking AKT and CXCR4 signalling.

Molecular oncology

Di Paolo D,Pontis F,Moro M,Centonze G,Bertolini G,Milione M,Mensah M,Segale M,Petraroia I,Borzi C,Suatoni P,Brignole C,Perri P,Ponzoni M,Pastorino U,Sozzi G,Fortunato O

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 25-9999-42) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 25-9999-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphoma.

Nature communications

Zhang S,Jiang VC,Han G,Hao D,Lian J,Liu Y,Zhang R,McIntosh J,Wang R,Dang M,Dai E,Wang Y,Santos D,Badillo M,Leeming A,Chen Z,Hartig K,Bigcal J,Zhou J,Kanagal-Shamanna R,Ok CY,Lee H,Steiner RE,Zhang J,Song X,Nair R,Ahmed S,Rodriquez A,Thirumurthi S,Jain P,Wagner-Bartak N,Hill H,Nomie K,Flowers C,Futreal A,Wang L,Wang M

25-9999-42 was used in Flow Cytometry to demonstrate that due to 17q gain, BIRC5/survivin expression is upregulated in resistant MCL tumor cells and targeting BIRC5 results in marked tumor inhibition in preclinical models.

Mon May 17 00:00:00 EDT 2021

Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphoma.

Nature communications

Mon May 17 00:00:00 EDT 2021

Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

International journal of inflammation

Genkel V,Dolgushin I,Baturina I,Savochkina A,Kuznetsova A,Pykhova L,Shaposhnik I

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 25-9999-42) in Flow Cytometry

Sun Apr 24 00:00:00 EDT 2022

Enhanced CXCR4 Expression of Human CD8^Low T Lymphocytes Is Driven by S1P₄.

Frontiers in immunology

Burkard T,Dreis C,Herrero San Juan M,Huhn M,Weigert A,Pfeilschifter JM,Radeke HH

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 25-9999-42) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Circulating Progenitor Cells in Patients With Coronary Artery Disease and Renal Insufficiency.

JACC. Basic to translational science

Mehta A,Tahhan AS,Liu C,Dhindsa DS,Nayak A,Hooda A,Moazzami K,Islam SJ,Rogers SC,Almuwaqqat Z,Mokhtari A,Hesaroieh I,Ko YA,Waller EK,Quyyumi AA

25-9999-42 was used in Flow Cytometry to investigate: 1) the association between renal insufficiency, age, and circulating progenitor cell counts; and 2) the predictive value of circulating progenitor cell among patients with coronary artery disease and renal insufficiency.

Sat Aug 01 00:00:00 EDT 2020

Circulating Progenitor Cells in Patients With Coronary Artery Disease and Renal Insufficiency.

JACC. Basic to translational science

Mehta A,Tahhan AS,Liu C,Dhindsa DS,Nayak A,Hooda A,Moazzami K,Islam SJ,Rogers SC,Almuwaqqat Z,Mokhtari A,Hesaroieh I,Ko YA,Waller EK,Quyyumi AA

Sat Aug 01 00:00:00 EDT 2020

Modulatory effects of trophoblast-secreted CXCL12 on the migration and invasion of human first-trimester decidual epithelial cells are mediated by CXCR4 rather than CXCR7.

Reproductive biology and endocrinology : RB&E

Zheng J,Wang H,Zhou W

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 25-9999-42) in Flow Cytometry

Fri Mar 02 00:00:00 EST 2018

The CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signaling.

Cell communication and signaling : CCS

Berning P,Schaefer C,Clemens D,Korsching E,Dirksen U,Potratz J

25-9999 was used in Flow cytometry/Cell sorting to investigate the CXCR4 receptor antagonist, plerixafor, for anti-cancer activity in Ewing sarcoma.

Fri May 18 00:00:00 EDT 2018

The CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signaling.

Cell communication and signaling : CCS

Berning P,Schaefer C,Clemens D,Korsching E,Dirksen U,Potratz J

25-9999 was used in Flow cytometry/Cell sorting to investigate the CXCR4 receptor antagonist, plerixafor, for anti-cancer activity in Ewing sarcoma.

Fri May 18 00:00:00 EDT 2018

Sex Differences in Circulating Progenitor Cells.

Journal of the American Heart Association

Topel ML,Hayek SS,Ko YA,Sandesara PB,Samman Tahhan A,Hesaroieh I,Mahar E,Martin GS,Waller EK,Quyyumi AA

Tue Oct 03 00:00:00 EDT 2017

Age and Human Regenerative Capacity Impact of Cardiovascular Risk Factors.

Circulation research

Al Mheid I,Hayek SS,Ko YA,Akbik F,Li Q,Ghasemzadeh N,Martin GS,Long Q,Hammadah M,Maziar Zafari A,Vaccarino V,Waller EK,Quyyumi AA

25-9999 was used in Flow cytometry/Cell sorting to investigate ageing of human endogenous reparative capacity and to clarify whether it is affected by presence of cardiovascular disease or its risk factors.

Fri Sep 16 00:00:00 EDT 2016

ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Berger RP,Sun YH,Kulik M,Lee JK,Nairn AV,Moremen KW,Pierce M,Dalton S

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 25-9999-42) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Circulating Progenitor Cells is Linked to Cognitive Decline in Healthy Adults.

The American journal of the medical sciences

Hajjar I,Goldstein FC,Waller EK,Moss LD,Quyyumi A

25-9999 was used in Flow cytometry/Cell sorting to assess the association between progenitor cells and cognition in a sample of healthy adults enrolled in a cohort study.

Mon Feb 01 00:00:00 EST 2016

In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells.

Methods in molecular biology (Clifton, N.J.)

Tian L,Prasad N,Jang YY

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 25-9999-42) in Flow Cytometry

Thu Aug 18 00:00:00 EDT 2016

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

25-9999 was used in Flow cytometry/Cell sorting to showcase the therapeutic potential of gene-editing coupled with stem cell technology for the treatment of recessive dystrophic epidermolysis bullosa.

Wed Sep 30 00:00:00 EDT 2020

Circulating CD34+ progenitor cells and risk of mortality in a population with coronary artery disease.

Circulation research

Patel RS,Li Q,Ghasemzadeh N,Eapen DJ,Moss LD,Janjua AU,Manocha P,Kassem HA,Veledar E,Samady H,Taylor WR,Zafari AM,Sperling L,Vaccarino V,Waller EK,Quyyumi AA

25-9999 was used in Flow cytometry/Cell sorting to investigate whether low numbers of progenitor cells associate with a greater risk of mortality in a population at high cardiovascular risk.

Fri Jan 16 00:00:00 EST 2015

Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.

Oncotarget

Ye SB,Li ZL,Luo DH,Huang BJ,Chen YS,Zhang XS,Cui J,Zeng YX,Li J

25-9999 was used in Flow cytometry/Cell sorting to show that tumour-derived exosomes promote tumour progression and T-cell dysfunction through regulation of exosomal microRNAs in human carcinomas.

Wed Jul 30 00:00:00 EDT 2014

Plerixafor as a chemosensitizing agent in pediatric acute lymphoblastic leukemia: efficacy and potential mechanisms of resistance to CXCR4 inhibition.

Oncotarget

Sison EA,Magoon D,Li L,Annesley CE,Rau RE,Small D,Brown P

25-9999 was used in Flow cytometry/Cell sorting to demonstrate that cells which upregulate surface CXCR4 in response to chemotherapy treatment are protected from chemotherapy-induced apoptosis when co-cultured with bone marrow stroma.

Wed Oct 15 00:00:00 EDT 2014

Effect of progenitor cell mobilization with granulocyte-macrophage colony-stimulating factor in patients with peripheral artery disease: a randomized clinical trial.

JAMA

Poole J,Mavromatis K,Binongo JN,Khan A,Li Q,Khayata M,Rocco E,Topel M,Zhang X,Brown C,Corriere MA,Murrow J,Sher S,Clement S,Ashraf K,Rashed A,Kabbany T,Neuman R,Morris A,Ali A,Hayek S,Oshinski J,Yoon YS,Waller EK,Quyyumi AA

25-9999 was used in Flow cytometry/Cell sorting to investigate whether therapy with granulocyte-macrophage colony-stimulating factor improves exercise capacity in patients with intermittent claudication.

Wed Dec 25 00:00:00 EST 2013

Elevated circulating angiogenic progenitors and white blood cells are associated with hypoxia-inducible angiogenic growth factors in children with sickle cell disease.

Anemia

Ofori-Acquah SF,Buchanan ID,Osunkwo I,Manlove-Simmons J,Lawal F,Quarshie A,Quyyumi AA,Gibbons GH,Gee BE

25-9999 was used in Flow cytometry/Cell sorting to contrast the immune cell population in children with and without haemoglobin SS, a form of sickle cell disease.

Thu Aug 23 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine receptor; CXC; CXC motif chemokine; CXC-R4; FB22; Fusin; HM89; HUMSTR; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPYRL; SDF-1 receptor; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor

View more View less

Gene Aliases: CD184; CXCR4; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; WHIMS

View more View less

UniProt ID: (Human) P61073

View more View less

Entrez Gene ID: (Human) 7852

View more View less

Function(s)

virus receptor activity actin binding G-protein coupled receptor activity protein binding drug binding coreceptor activity C-X-C chemokine receptor activity cytokine binding ubiquitin protein ligase binding myosin light chain binding ubiquitin binding

Process(es)

activation of MAPK activity response to hypoxia neuron migration epithelial cell development dendritic cell chemotaxis apoptotic process inflammatory response G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration neuron recognition response to virus fusion of virus membrane with host plasma membrane calcium-mediated signaling telencephalon cell migration entry into host cell regulation of cell migration regulation of programmed cell death myelin maintenance positive regulation of oligodendrocyte differentiation regulation of chemotaxis regulation of calcium ion transport cardiac muscle contraction chemokine-mediated signaling pathway cellular response to cytokine stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD184 (CXCR4) Monoclonal Antibody (12G5), PE-Cyanine7, eBioscience™

CD184 (CXCR4) Antibody (25-9999-42) in Flow

Product Details

25-9999-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!