Invitrogen

CD19 Monoclonal Antibody (MB19-1), PE, eBioscience™

FIGURE: 1 / 1

CD19 Antibody (12-0191-82) in Flow

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or Anti-Mouse CD19 PE (filled histogram). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

12-0191-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgA, kappa

Class

Monoclonal

Type

Antibody

Clone

MB19-1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465577

Product Specific Information

Description: The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. Staining of B cells with MB19-1 and its conjugates is usually dimmer than the rat anti-mouse CD19 antibody, clone 6D5.

Applications Reported: The MB19-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The MB19-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Scientific reports

Tran A,Scholtes C,Songane M,Champagne C,Galarneau L,Levasseur MP,Fodil N,Dufour CR,Giguère V,Saleh M

12-0191-82 was used in Immunohistochemistry (Paraffin) to report a protective role of estrogen-related receptor alpha in the intestine.

Fri Jul 23 00:00:00 EDT 2021

Dectin-2 Deficiency Modulates Th1 Differentiation and Improves Wound Healing After Myocardial Infarction.

Circulation research

Yan X,Zhang H,Fan Q,Hu J,Tao R,Chen Q,Iwakura Y,Shen W,Lu L,Zhang Q,Zhang R

12-0191 was used in Flow cytometry/Cell sorting to determine whether Dectin-2 signalling is involved in the healing process and cardiac remodelling after myocardial infarction and to elucidate the underlying molecular mechanisms.

Fri Mar 31 00:00:00 EDT 2017

Double-stranded RNA promotes CTL-independent tumor cytolysis mediated by CD11b⁺Ly6G⁺ intratumor myeloid cells through the TICAM-1 signaling pathway.

Cell death and differentiation

Shime H,Matsumoto M,Seya T

12-0191 was used in Flow cytometry/Cell sorting to study the response of CD11b+Ly6G+ granulocytic myeloid-derived suppressor cells to polyI:C in an EL4 tumour implant model.

Wed Mar 01 00:00:00 EST 2017

Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization.

Journal of immunology (Baltimore, Md. : 1950)

Viladomiu M,Bassaganya-Riera J,Tubau-Juni N,Kronsteiner B,Leber A,Philipson CW,Zoccoli-Rodriguez V,Hontecillas R

12-0191 was used in Flow cytometry/Cell sorting to report the induction of IL-10-driven regulatory responses mediated by CD11b+F4/80hiCD64+CX3CR1+ MNPs that contribute to maintaining high levels of Helicobacter pylori loads in the stomach by modulating effector T cell responses at the gastric mucosa.

Sat Apr 15 00:00:00 EDT 2017

A critical role for cellular inhibitor of protein 2 (cIAP2) in colitis-associated colorectal cancer and intestinal homeostasis mediated by the inflammasome and survival pathways.

Mucosal immunology

Dagenais M,Dupaul-Chicoine J,Champagne C,Skeldon A,Morizot A,Saleh M

12-0191 was used in Immunofluorescence-paraffin to investigate the function of cellular inhibitors of apoptosis proteins in intestinal cancer and mechanisms in disease pathogenesis.

Fri Jan 01 00:00:00 EST 2016

Expression of a single ICAM-1 isoform on T cells is sufficient for development of experimental autoimmune encephalomyelitis.

European journal of immunology

Bullard DC,Hu X,Crawford D,McDonald K,Ramos TN,Barnum SR

12-0191 was used in Flow cytometry/Cell sorting to assess the role of a particular ICAM-1 isoform expressed by T-cells in the development of of experimental autoimmune encephalomyelitis.

Tue Apr 01 00:00:00 EDT 2014

Minor cell-death defects but reduced tumor latency in mice lacking the BH3-only proteins Bad and Bmf.

Oncogene

Baumgartner F,Woess C,Pedit V,Tzankov A,Labi V,Villunger A

12-0191 was used in Flow cytometry/Cell sorting to show that Bad and Bmf co-regulate lymphocyte homeostasis and limit spontaneous transformation in mice.

Thu Jan 31 00:00:00 EST 2013

IKKα-mediated signaling circuitry regulates early B lymphopoiesis during hematopoiesis.

Blood

Balkhi MY,Willette-Brown J,Zhu F,Chen Z,Liu S,Guttridge DC,Karin M,Hu Y

12-0191-82 was used in Flow Cytometry to demonstrate that IKKα regulates B-lineage commitment via combined canonical and noncanonical NF-κB transcriptional activities to target Pax5 expression during hematopoiesis.

Thu Jun 07 00:00:00 EDT 2012

Schistosomes induce regulatory features in human and mouse CD1d(hi) B cells: inhibition of allergic inflammation by IL-10 and regulatory T cells.

PloS one

van der Vlugt LE,Labuda LA,Ozir-Fazalalikhan A,Lievers E,Gloudemans AK,Liu KY,Barr TA,Sparwasser T,Boon L,Ngoa UA,Feugap EN,Adegnika AA,Kremsner PG,Gray D,Yazdanbakhsh M,Smits HH

12-0191 was used in Flow cytometry/Cell sorting to investigate the effect of Schistosoma mansoni infection on allergic airway inflammation, showing it was inhibited by IL-10 produced by CD1d(hi) B cells.

Tue Jul 31 00:00:00 EDT 2012

Rara haploinsufficiency modestly influences the phenotype of acute promyelocytic leukemia in mice.

Blood

Welch JS,Klco JM,Varghese N,Nagarajan R,Ley TJ

12-0191 was used in Flow cytometry/Cell sorting to test the prediction that RARA (retinoic acid receptor alpha) haploinsufficiency would contribute to acute promyelocytic leukemia (APL) pathogenesis.

Thu Feb 24 00:00:00 EST 2011

PML-RARA can increase hematopoietic self-renewal without causing a myeloproliferative disease in mice.

The Journal of clinical investigation

Welch JS,Yuan W,Ley TJ

12-0191 was used in Flow cytometry/Cell sorting to study a platform for more accurately dissecting the early events in acute promyelocytic leukemia pathogenesis.

Fri Apr 01 00:00:00 EDT 2011

Low CD4/CD8 T-cell ratio associated with inflammatory arthropathy in human T-cell leukemia virus type I Tax transgenic mice.

PloS one

Ohsugi T,Kumasaka T

12-0191 was used in Flow cytometry/Cell sorting to identify the primary T-cell subsets involved in the development of arthropathy in Tax transgenic mice.

Fri Apr 01 00:00:00 EDT 2011

Nylon wool purification alters the activation of T cells.

Molecular immunology

Wohler JE,Barnum SR

12-0191 was used in Flow cytometry/Cell sorting to examine the effects of nylon wool purification on T cell proliferation, activation marker expression and cytokine production.

Sun Feb 01 00:00:00 EST 2009

Recruitment and in situ renewal regulate rapid accumulation of CD11c+ cells in the lung following intranasal superantigen challenge.

International archives of allergy and immunology

Muralimohan G,Rossi RJ,Vella AT

12-0191 was used in Flow cytometry/Cell sorting to investigate how staphylococcal enterotoxins regulate innate immune cells, showing that it leads to rapid accumulation of CD11c+ cells in the lung following superantigen challenge.

Tue Sep 09 00:00:00 EDT 2008

Hierarchy of Notch-Delta interactions promoting T cell lineage commitment and maturation.

The Journal of experimental medicine

Besseyrias V,Fiorini E,Strobl LJ,Zimber-Strobl U,Dumortier A,Koch U,Arcangeli ML,Ezine S,Macdonald HR,Radtke F

12-0191 was used in Flow cytometry/Cell sorting to study the Notch-Delta interactions that promote T cell development.

Mon Feb 19 00:00:00 EST 2007

Induction of robust immune responses against human immunodeficiency virus is supported by the inherent tropism of adeno-associated virus type 5 for dendritic cells.

Journal of virology

Xin KQ,Mizukami H,Urabe M,Toda Y,Shinoda K,Yoshida A,Oomura K,Kojima Y,Ichino M,Klinman D,Ozawa K,Okuda K

12-0191 was used in Flow cytometry/Cell sorting to investigate the ability of different adeno-associated virus vectors expressing the human immunodeficiency virus to induce a robust immune response, and the factors which affect this.

Fri Dec 01 00:00:00 EST 2006

Delayed, asynchronous, and reversible T-lineage specification induced by Notch/Delta signaling.

Genes & development

Taghon TN,David ES,Zúñiga-Pflücker JC,Rothenberg EV

12-0191 was used in Flow cytometry/Cell sorting to study how Notch/Delta signaling is not sufficient for T-lineage specification and commitment, but can be permissive for the maintenance and proliferation of uncommitted progenitors.

Fri Apr 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: B-lymphocyte antigen CD19; CD19; Differentiation antigen CD19; Leu-12

View more View less

Gene Aliases: AW495831; Cd19

View more View less

UniProt ID: (Mouse) P25918

View more View less

Entrez Gene ID: (Mouse) 12478

View more View less

Process(es)

chromosome breakage B cell receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD19 Monoclonal Antibody (MB19-1), PE, eBioscience™

CD19 Antibody (12-0191-82) in Flow

Product Details

12-0191-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!