Invitrogen

CD1d Monoclonal Antibody (1B1), PE, eBioscience™

FIGURE: 1 / 5

CD1d Antibody (12-0011-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2b K Isotype Control PE (Product # 12-4031-82) (left) or 0.06 µg of Anti-Mouse CD1d PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-0011-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1B1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465483

Product Specific Information

Description: The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural homology to MHC class I molecules. While similar to MHC Class I, CD1d associates with beta2m, functionally CD1d is similar to MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes. 1B1 detects beta2m associated CD1d.

Applications Reported: The 1B1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 1B1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins. The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Methotrexate-loaded nanoparticles ameliorate experimental model of autoimmune arthritis by regulating the balance of interleukin-17-producing T cells and regulatory T cells.

Journal of translational medicine

Park JS,Lee D,Yang S,Jeong HY,Na HS,Cho KH,Choi J,Koo H,Cho ML,Park SH

Published figure using CD1d monoclonal antibody (Product # 12-0011-82) in Flow Cytometry

Fri Feb 11 00:00:00 EST 2022

Methotrexate-loaded nanoparticles ameliorate experimental model of autoimmune arthritis by regulating the balance of interleukin-17-producing T cells and regulatory T cells.

Journal of translational medicine

Park JS,Lee D,Yang S,Jeong HY,Na HS,Cho KH,Choi J,Koo H,Cho ML,Park SH

Published figure using CD1d monoclonal antibody (Product # 12-0011-82) in Flow Cytometry

Fri Feb 11 00:00:00 EST 2022

Environmental activation of a hypothalamic BDNF-adipocyte IL-15 axis regulates adipose-natural killer cells.

Brain, behavior, and immunity

Bergin SM,Xiao R,Huang W,Judd CRT,Liu X,Mansour AG,Queen N,Widstrom KJ,Caligiuri MA,Cao L

12-0011-82 was used in Flow cytometry/Cell sorting to show that hypothalamic brain-derived neurotrophic factor upregulates IL-15 production in adipocytes via sympathetic β-adrenergic signaling.

Thu Jul 01 00:00:00 EDT 2021

Induction of Foxp3 and activation of Tregs by HSP gp96 for treatment of autoimmune diseases.

iScience

Xu Y,Liu E,Xie X,Wang J,Zheng H,Ju Y,Chen L,Li C,Zhou X,Li Z,Li X,Meng S

12-0011-82 was used in Flow cytometry/Cell sorting to show that gp96 immunization restricted antibody-induced and Th-induced autoimmune diseases by integrating Treg expansion and activation, indicating its potential clinical usefulness against autoimmune diseases.

Fri Dec 17 00:00:00 EST 2021

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

12-0011-82 was used in Flow Cytometry to indicate a potential alternative to IVIg for therapeutic intervention in case of pregnancy failures.

Mon Jun 29 00:00:00 EDT 2020

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

12-0011-82 was used in Flow Cytometry to indicate a potential alternative to IVIg for therapeutic intervention in case of pregnancy failures.

Mon Jun 29 00:00:00 EDT 2020

Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome.

Nature communications

Komban RJ,Strömberg A,Biram A,Cervin J,Lebrero-Fernández C,Mabbott N,Yrlid U,Shulman Z,Bemark M,Lycke N

12-0011-82 was used in Flow Cytometry to report a M cell-B cell antigen-specific transporting pathway in PP that is independent of DC. We propose that this antigen transporting pathway has a critical role in gut IgA responses, and should be taken into account when developing mucosal vaccines.

Mon Jun 03 00:00:00 EDT 2019

The c-Myc/miR17-92/PTEN Axis Tunes PI3K Activity to Control Expression of Recombination Activating Genes in Early B Cell Development.

Frontiers in immunology

Benhamou D,Labi V,Getahun A,Benchetrit E,Dowery R,Rajewsky K,Cambier JC,Melamed D

12-0011 was used in Flow cytometry/Cell sorting to elucidate that the c-Myc/miR17-92/PTEN axis tunes PI3K activity to control the expression of recombination activating genes in proB cells.

Wed Oct 02 00:00:00 EDT 2019

Estrogen protection against EAE modulates the microbiota and mucosal-associated regulatory cells.

Journal of neuroimmunology

Benedek G,Zhang J,Nguyen H,Kent G,Seifert HA,Davin S,Stauffer P,Vandenbark AA,Karstens L,Asquith M,Offner H

Fri Sep 15 00:00:00 EDT 2017

Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis.

PloS one

Komegae EN,Souza TA,Grund LZ,Lima C,Lopes-Ferreira M

Published figure using CD1d monoclonal antibody (Product # 12-0011-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Tissue-specific DNA demethylation is required for proper B-cell differentiation and function.

Proceedings of the National Academy of Sciences of the United States of America

Orlanski S,Labi V,Reizel Y,Spiro A,Lichtenstein M,Levin-Klein R,Koralov SB,Skversky Y,Rajewsky K,Cedar H,Bergman Y

12-0011 was used in Flow cytometry/Cell sorting to determine the effect of DNA demethylation at specific sites on B cell differentiation and function.

Tue May 03 00:00:00 EDT 2016

Histone deacetylase inhibitors enhance CD1d-dependent NKT cell responses to lymphoma.

Cancer immunology, immunotherapy : CII

Tiper IV,Webb TJ

12-0011 was used in Flow cytometry/Cell sorting to demonstrate the efficacy of HDACi in restoring anti-tumor responses to mantle cell lymphoma through both cell-intrinsic and cell-extrinsic mechanisms.

Tue Nov 01 00:00:00 EDT 2016

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

12-0011 was used in Flow cytometry/Cell sorting to indicate a close link between NFATc1 and IL-10 expression in B cells, suggesting that NFATc1 may be a potential target to treat human psoriasis.

Wed May 25 00:00:00 EDT 2016

CXCL16-positive dendritic cells enhance invariant natural killer T cell-dependent IFNγ production and tumor control.

Oncoimmunology

Veinotte L,Gebremeskel S,Johnston B

12-0011 was used in Flow cytometry/Cell sorting to study the co-stimulatory role of CXCR6/CXCL16 interactions in glycolipid-dependent invariant natural killer T (iNKT) cell activation and tumour control, showing that CXCL16-positive dendritic cells enhance iNKT cell-dependent IFNγ production and tumor control.

Wed Jun 01 00:00:00 EDT 2016

BAFF activation of the ERK5 MAP kinase pathway regulates B cell survival.

The Journal of experimental medicine

Jacque E,Schweighoffer E,Tybulewicz VL,Ley SC

12-0011 was used in Flow cytometry/Cell sorting to reveal a potentially critical role for the MEK5-ERK5 MAP kinase signalling pathway in BAFF-induced mature B cell survival and homeostatic maintenance of B2 cell numbers.

Mon Jun 01 00:00:00 EDT 2015

Obesity enhances Th2 inflammatory response via natural killer T cells in a murine model of allergic asthma.

International journal of clinical and experimental medicine

Chen YP,Zhang JH,Li CQ,Sun QX,Jiang XH

12-0011 was used in Flow cytometry/Cell sorting to investigate the mechanisms connecting the development of asthma and obesity.

Wed Dec 02 00:00:00 EST 2015

Oral combined therapy with probiotics and alloantigen induces B cell-dependent long-lasting specific tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Mercadante AC,Perobelli SM,Alves AP,Gonçalves-Silva T,Mello W,Gomes-Santos AC,Miyoshi A,Azevedo V,Faria AM,Bonomo A

12-0011 was used in Flow cytometry/Cell sorting to suggest that combined therapy is a promising strategy for prevention of GVHD with preservation of graft-versus-leukemia in allogenic HSCT.

Sat Feb 15 00:00:00 EST 2014

The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice.

The Journal of biological chemistry

Koenig PA,Nicholls PK,Schmidt FI,Hagiwara M,Maruyama T,Frydman GH,Watson N,Page DC,Ploegh HL

12-0011 was used in Flow cytometry/Cell sorting to identify an essential function for the ubiquitin-proteasome-system in spermiogenesis.

Fri Dec 12 00:00:00 EST 2014

AID-expressing germinal center B cells cluster normally within lymph node follicles in the absence of FDC-M1+ CD35+ follicular dendritic cells but dissipate prematurely.

Journal of immunology (Baltimore, Md. : 1950)

Boulianne B,Le MX,Ward LA,Meng L,Haddad D,Li C,Martin A,Gommerman JL

12-0011 was used in Flow cytometry/Cell sorting to provide a spatial-temporal analysis of Ag-specific B cell activation and AID expression in the context of a peripheral lymph node that lacks FDC-M1(+) CD35(+) FDC and other LT-sensitive cell types.

Fri Nov 01 00:00:00 EDT 2013

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

12-0011 was used in Flow cytometry/Cell sorting to demonstrate that nuclear export of IκBα is essential in maintaining constitutive, canonical, and noncanonical NF-κB activation potentials in mature B cells in vivo.

Fri Feb 25 00:00:00 EST 2011

Blockade of B7-H1 (programmed death ligand 1) enhances humoral immunity by positively regulating the generation of T follicular helper cells.

Journal of immunology (Baltimore, Md. : 1950)

Hams E,McCarron MJ,Amu S,Yagita H,Azuma M,Chen L,Fallon PG

12-0011 was used in Flow cytometry/Cell sorting to demonstrate that the coinhibitory B7-H1/programmed death 1 pathway can limit the expansion of T(FH) cells and constrain Ag-specific Ig responses.

Sun May 15 00:00:00 EDT 2011

An anti-inflammatory role for carbon monoxide and heme oxygenase-1 in chronic Th2-mediated murine colitis.

Journal of immunology (Baltimore, Md. : 1950)

Sheikh SZ,Hegazi RA,Kobayashi T,Onyiah JC,Russo SM,Matsuoka K,Sepulveda AR,Li F,Otterbein LE,Plevy SE

12-0011 was used in Flow cytometry/Cell sorting to investigate homeostatic and therapeutic effects of CO and HO-1 in chronic colonic inflammation in TCR-α-deficient (-/-) mice.

Sun May 01 00:00:00 EDT 2011

Regulation of the B cell receptor repertoire and self-reactivity by BAFF.

Journal of immunology (Baltimore, Md. : 1950)

Ota M,Duong BH,Torkamani A,Doyle CM,Gavin AL,Ota T,Nemazee D

12-0011 was used in Flow cytometry/Cell sorting to highlight the negative effects of excess BAFF signalling to display the positive implications of BAFF-depleting therapy.

Fri Oct 01 00:00:00 EDT 2010

Stromal cell independent B cell development in vitro: generation and recovery of autoreactive clones.

Journal of immunological methods

Holl TM,Haynes BF,Kelsoe G

12-0011 was used in Flow cytometry/Cell sorting to describe a stromal cell independent culture system that efficiently supports pro-B cell to IgM+ B cell development with near normal levels of IgH and Igkappa diversity.

Wed Mar 31 00:00:00 EDT 2010

Absence of IFN-beta impairs antigen presentation capacity of splenic dendritic cells via down-regulation of heat shock protein 70.

Journal of immunology (Baltimore, Md. : 1950)

Zietara N,Łyszkiewicz M,Gekara N,Puchałka J,Dos Santos VA,Hunt CR,Pandita TK,Lienenklaus S,Weiss S

12-0011 was used in Flow cytometry/Cell sorting to investigate the role of low level production of IFN, showing that its absence impairs antigen presentation capacity of splenic dendritic cells.

Wed Jul 15 00:00:00 EDT 2009

Functional maturation of lamina propria dendritic cells by activation of NKT cells mediates the abrogation of oral tolerance.

European journal of immunology

Chang JH,Lee JM,Youn HJ,Lee KA,Chung Y,Lee AY,Kweon MN,Kim HY,Taniguchi M,Kang CY

12-0011 was used in Flow cytometry/Cell sorting to examine the essential factor responsible for the disruption of oral tolerance.

Wed Oct 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: Antigen-presenting glycoprotein CD1d1; CD1d.1; T-cell surface glycoprotein CD1d1

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Gene Aliases: AI747460; CD1.1; Cd1a; Cd1d; Cd1d1; Ly-38

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UniProt ID: (Mouse) P11609

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Entrez Gene ID: (Mouse) 12479

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Function(s)

protein binding beta-2-microglobulin binding lipid antigen binding endogenous lipid antigen binding exogenous lipid antigen binding histone binding T cell receptor binding cell adhesion molecule binding lipopeptide binding major histocompatibility complex protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD1d Monoclonal Antibody (1B1), PE, eBioscience™

CD1d Antibody (12-0011-82) in Flow

Product Details

12-0011-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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