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Invitrogen
CD268 (BAFF Receptor) Monoclonal Antibody (8A7), Functional Grade, eBioscience™
-Functional-Grade-Flow-20170216130641.jpg?time=20240507 "CD268 (BAFF Receptor) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 10
CD268 (BAFF Receptor) Antibody (16-9117-82) in Flow
Staining of normal human peripheral blood cells with 0.125 µg of Mouse IgG2a K Isotype Control Functional Grade Purified (Product # 16-4724-85) (open histogram) or 0.125 µg of Anti-Human CD268 (BAFF Receptor) Functional Grade Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte gate were used for analysis.
-Functional-Grade-Flow-20170216130641.jpg?time=20240507)
-Functional-Grade-Flow-20170216130641.jpg?time=20240507?time=20240507 "CD268 (BAFF Receptor) Antibody (16-9117-82) in Flow")
Product Details
16-9117-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
8A7
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_469270
Product Specific Information
Description: The 8A7 monoclonal antibody reacts with the human BAFF receptor, also known as B lymphocyte stimulator (BLyS protein) receptor, which is expressed on B cells. The ligand for this receptor, BAFF (B cell-activating factor of the TNF family) is a B cell survival factor and regulates CD21/35 and CD23 expression. Interaction of this ligand with its receptor causes elevated CD21/35 and CD23 expression, whereas receptor blockade has been shown to down-modulate expression.
Applications Reported: The 8A7 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in functional assays.
Applications Tested: The 8A7 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFFR, is a protein in humans is encoded by the TNFRSF13C gene. The BAFFR gene is mapped to chromosome 22q13. 1-q13. 31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B cell-activating factor receptor; B-cell-activating factor receptor; BAFF receptor; BAFF-R; BLyS receptor 3; CD268; Tumor necrosis factor receptor superfamily member 13C; tumor necrosis factor receptor superfamily, member 13C
Gene Aliases: BAFF-R; BAFFR; BR3; BROMIX; CD268; CVID4; prolixin; TNFRSF13C
UniProt ID: (Human) Q96RJ3
Entrez Gene ID: (Human) 115650
B cell homeostasis
adaptive immune response
positive regulation of germinal center formation
positive regulation of B cell proliferation
T cell costimulation
B cell costimulation
tumor necrosis factor-mediated signaling pathway
positive regulation of T cell proliferation
positive regulation of interferon-gamma biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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