Invitrogen

CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Biotin, eBioscience™

CD274 (PD-L1, B7-H1) Antibody in Flow Cytometry (Flow)

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CD274 (PD-L1, B7-H1) Antibody (13-5982-82) in Flow

Staining of C57Bl/6 splenocytes with 0.125 µg of Rat IgG2a Isotype Control Biotin (Product # 13-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD274 (B7-H1) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD274 (PD-L1, B7-H1) Antibody in Western Blot (WB)

CD274 (PD-L1, B7-H1) Antibody in Immunohistochemistry (IHC)

CD274 (PD-L1, B7-H1) Antibody in Immunocytochemistry (ICC/IF)

CD274 (PD-L1, B7-H1) Antibody in Neutralization (Neu)

CD274 (PD-L1, B7-H1) Antibody in Functional Assay (Functional)

Product Image Gallery

Product Details

13-5982-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 34 publications 34 publications

Neutralization (Neu)

View 5 publications 5 publications

Functional Assay (Functional)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, lambda

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MIH5

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466837

Product Specific Information

Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.

Applications Reported: The MIH5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

AR-A014418 regulates intronic polyadenylation and transcription of PD-L1 through inhibiting CDK12 and CDK13 in tumor cells.

Journal for immunotherapy of cancer

Zhang G,Lan B,Zhang X,Lin M,Liu Y,Chen J,Guo F

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

Sustained release of therapeutic gene by injectable hydrogel for hepatocellular carcinoma.

International journal of pharmaceutics: X

Xu S,Cai J,Cheng H,Wang W

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2023

Contribution of Mesenchymal Stem Cells from Obese Adipose Tissue to PD-L1 Over-Expression and Breast Cancer Progression through Pathogenic Th17 Cell Activation.

Cancers

Blangero F,Robert M,Andraud T,Dumontet C,Vidal H,Eljaafari A

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Mon May 29 00:00:00 EDT 2023

BRAF D594A mutation defines a unique biological and immuno-modulatory subgroup associated with functional CD8⁺ T cell infiltration in colorectal cancer.

Journal of translational medicine

Li W,Zhao C,Li W,Gong Y,Ma K,Lu Y,Liu X,Zhang L,Guo F

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Wed Oct 18 00:00:00 EDT 2023

IFNAR blockade synergizes with oncolytic VSV to prevent virus-mediated PD-L1 expression and promote antitumor T cell activity.

Molecular therapy oncolytics

El-Sayes N,Walsh S,Vito A,Reihani A,Ask K,Wan Y,Mossman K

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Immunohistochemistry

Thu Jun 16 00:00:00 EDT 2022

Modification of PLAC8 by UFM1 affects tumorous proliferation and immune response by impacting PD-L1 levels in triple-negative breast cancer.

Journal for immunotherapy of cancer

Mao M,Chen Y,Yang J,Cheng Y,Xu L,Ji F,Zhou J,Zhang X,Li Z,Chen C,Ju S,Zhang J,Wang L

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Heterogeneity of tyrosine-based melanin anabolism regulates pulmonary and cerebral organotropic colonization microenvironment of melanoma cells.

Theranostics

Wang X,Chen Y,Lan B,Wang Y,Lin W,Jiang X,Ye J,Shang B,Feng C,Liu J,Zhai J,Xu M,Li Q,Lin L,Hu M,Zheng F,Chen L,Shao C,Wang Y,Shi Y

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2022

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

5-Azacytidine-Mediated Modulation of the Immune Microenvironment in Murine Acute Myeloid Leukemia.

Cancers

Ebelt ND,Manuel ER

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Sun Dec 25 00:00:00 EST 2022

Scoring model based on the signature of non-m6A-related neoantigen-coding lncRNAs assists in immune microenvironment analysis and TCR-neoantigen pair selection in gliomas.

Journal of translational medicine

Zhao W,Wu Y,Zhao F,Xue Z,Liu W,Cao Z,Zhao Z,Huang B,Han M,Li X

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Immunohistochemistry

Sat Oct 29 00:00:00 EDT 2022

Isotope tracing reveals distinct substrate preference in murine melanoma subtypes with differing anti-tumor immunity.

Cancer & metabolism

Zhang X,Halberstam AA,Zhu W,Leitner BP,Thakral D,Bosenberg MW,Perry RJ

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Multiscale imaging of therapeutic anti-PD-L1 antibody localization using molecularly defined imaging agents.

Journal of nanobiotechnology

Hagemans IM,Wierstra PJ,Steuten K,Molkenboer-Kuenen JDM,van Dalen D,Ter Beest M,van der Schoot JMS,Ilina O,Gotthardt M,Figdor CG,Scheeren FA,Heskamp S,Verdoes M

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Wed Feb 02 00:00:00 EST 2022

Radiation-induced abscopal effect and its enhancement by programmed cell death 1 blockade in the hepatocellular carcinoma: A murine model study.

Clinical and molecular hepatology

Yoo GS,Ahn WG,Kim SY,Kang W,Choi C,Park HC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Oncolytic herpes simplex virus HF10 (canerpaturev) promotes accumulation of CD8⁺ PD-1^- tumor-infiltrating T cells in PD-L1-enriched tumor microenvironment.

International journal of cancer

Eissa IR,Mukoyama N,Abdelmoneim M,Naoe Y,Matsumura S,Bustos-Villalobos I,Ichinose T,Miyajima N,Morimoto D,Tanaka M,Fujimoto Y,Sone M,Kodera Y,Kasuya H

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Preclinical characterization of bemarituzumab, an anti-FGFR2b antibody for the treatment of cancer.

mAbs

Xiang H,Chan AG,Ahene A,Bellovin DI,Deng R,Hsu AW,Jeffry U,Palencia S,Powers J,Zanghi J,Collins H

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Immunocytochemistry

Mon Apr 04 00:00:00 EDT 2022

Collagenase-Expressing Salmonella Targets Major Collagens in Pancreatic Cancer Leading to Reductions in Immunosuppressive Subsets and Tumor Growth.

Cancers

Ebelt ND,Zamloot V,Zuniga E,Passi KB,Sobocinski LJ,Young CA,Blazar BR,Manuel ER

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

N-cadherin inhibitor creates a microenvironment that protect TILs from immune checkpoints and Treg cells.

Journal for immunotherapy of cancer

Sun Y,Jing J,Xu H,Xu L,Hu H,Tang C,Liu S,Wei Q,Duan R,Guo J,Yang L

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Neutralization

Mon Mar 01 00:00:00 EST 2021

N-cadherin inhibitor creates a microenvironment that protect TILs from immune checkpoints and Treg cells.

Journal for immunotherapy of cancer

Sun Y,Jing J,Xu H,Xu L,Hu H,Tang C,Liu S,Wei Q,Duan R,Guo J,Yang L

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Neutralization

Mon Mar 01 00:00:00 EST 2021

Ketogenic diet and ketone bodies enhance the anticancer effects of PD-1 blockade.

JCI insight

Ferrere G,Tidjani Alou M,Liu P,Goubet AG,Fidelle M,Kepp O,Durand S,Iebba V,Fluckiger A,Daillère R,Thelemaque C,Grajeda-Iglesias C,Alves Costa Silva C,Aprahamian F,Lefevre D,Zhao L,Ryffel B,Colomba E,Arnedos M,Drubay D,Rauber C,Raoult D,Asnicar F,Spector T,Segata N,Derosa L,Kroemer G,Zitvogel L

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Mon Jan 25 00:00:00 EST 2021

Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8⁺ T cell exclusion.

Journal of experimental & clinical cancer research : CR

Fang W,Zhou T,Shi H,Yao M,Zhang D,Qian H,Zeng Q,Wang Y,Jin F,Chai C,Chen T

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Neutralization

Mon Jan 04 00:00:00 EST 2021

USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade.

Nature communications

Yang Z,Xu G,Wang B,Liu Y,Zhang L,Jing T,Tang M,Xu X,Jiao K,Xiang L,Fu Y,Tang D,Zhang X,Jin W,Zhuang G,Zhao X,Liu Y

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Wed Aug 11 00:00:00 EDT 2021

Antigen-Capturing Mesoporous Silica Nanoparticles Enhance the Radiation-Induced Abscopal Effect in Murine Hepatocellular Carcinoma Hepa1-6 Models.

Pharmaceutics

Yang K,Choi C,Cho H,Ahn WG,Kim SY,Shin SW,Kim Y,Jang T,Lee N,Park HC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Downregulation of PD-L1 via amide analogues of brefelamide: Alternatives to antibody-based cancer immunotherapy.

Experimental and therapeutic medicine

Zhang J,Yamada O,Kida S,Murase S,Hattori T,Oshima Y,Kikuchi H

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation.

Journal for immunotherapy of cancer

Wu B,Chiang HC,Sun X,Yuan B,Mitra P,Hu Y,Curiel TJ,Li R

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.

Journal for immunotherapy of cancer

Neuwelt AJ,Kimball AK,Johnson AM,Arnold BW,Bullock BL,Kaspar RE,Kleczko EK,Kwak JW,Wu MH,Heasley LE,Doebele RC,Li HY,Nemenoff RA,Clambey ET

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

CD279 mediates the homeostasis and survival of regulatory T cells by enhancing T cell and macrophage interactions.

FEBS open bio

Liu Y,Gao Y,Hao H,Hou T

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Functional assay

Mon Jun 01 00:00:00 EDT 2020

CD279 mediates the homeostasis and survival of regulatory T cells by enhancing T cell and macrophage interactions.

FEBS open bio

Liu Y,Gao Y,Hao H,Hou T

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Functional assay

Mon Jun 01 00:00:00 EDT 2020

Nuclear imaging-guided PD-L1 blockade therapy increases effectiveness of cancer immunotherapy.

Journal for immunotherapy of cancer

Gao H,Wu Y,Shi J,Zhang X,Liu T,Hu B,Jia B,Wan Y,Liu Z,Wang F

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Epigenetically modulated FOXM1 suppresses dendritic cell maturation in pancreatic cancer and colon cancer.

Molecular oncology

Zhou Z,Chen H,Xie R,Wang H,Li S,Xu Q,Xu N,Cheng Q,Qian Y,Huang R,Shao Z,Xiang M

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

Inhibition of ATM reverses EMT and decreases metastatic potential of cisplatin-resistant lung cancer cells through JAK/STAT3/PD-L1 pathway.

Journal of experimental & clinical cancer research : CR

Shen M,Xu Z,Xu W,Jiang K,Zhang F,Ding Q,Xu Z,Chen Y

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Neutralization

Mon Apr 08 00:00:00 EDT 2019

Persistence of Integrase-Deficient Lentiviral Vectors Correlates with the Induction of STING-Independent CD8⁺ T Cell Responses.

Cell reports

Cousin C,Oberkampf M,Felix T,Rosenbaum P,Weil R,Fabrega S,Morante V,Negri D,Cara A,Dadaglio G,Leclerc C

13-5982 was used in Flow cytometry/Cell sorting to demonstrate that integrase-deficient lentiviral vectors can trigger persistent adaptive response by the mediation of a weak and transient innate response alongside the persistence of the vector in tissues.

Tue Jan 29 00:00:00 EST 2019

Obesity Drives STAT-1-Dependent NASH and STAT-3-Dependent HCC.

Cell

Grohmann M,Wiede F,Dodd GT,Gurzov EN,Ooi GJ,Butt T,Rasmiena AA,Kaur S,Gulati T,Goh PK,Treloar AE,Archer S,Brown WA,Muller M,Watt MJ,Ohara O,McLean CA,Tiganis T

13-5982-82 was used in Flow Cytometry to reveal how obesity-associated hepatic oxidative stress can independently contribute to the pathogenesis of non-alcoholic steatohepatitis, fibrosis, and hepatocellular carcinoma.

Thu Nov 15 00:00:00 EST 2018

PD-L1 in tumor microenvironment mediates resistance to oncolytic immunotherapy.

The Journal of clinical investigation

Zamarin D,Ricca JM,Sadekova S,Oseledchyk A,Yu Y,Blumenschein WM,Wong J,Gigoux M,Merghoub T,Wolchok JD

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Mon Apr 02 00:00:00 EDT 2018

ATM‑JAK‑PD‑L1 signaling pathway inhibition decreases EMT and metastasis of androgen‑independent prostate cancer.

Molecular medicine reports

Zhang L,Xu LJ,Zhu J,Li J,Xue BX,Gao J,Sun CY,Zang YC,Zhou YB,Yang DR,Shan YX

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Immunofluorescence

Tue May 01 00:00:00 EDT 2018

Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Nature communications

Sun C,Lan P,Han Q,Huang M,Zhang Z,Xu G,Song J,Wang J,Wei H,Zhang J,Sun R,Zhang C,Tian Z

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Wed Mar 28 00:00:00 EDT 2018

Oncogenic RAS Signaling Promotes Tumor Immunoresistance by Stabilizing PD-L1 mRNA.

Immunity

Coelho MA,de Carné Trécesson S,Rana S,Zecchin D,Moore C,Molina-Arcas M,East P,Spencer-Dene B,Nye E,Barnouin K,Snijders AP,Lai WS,Blackshear PJ,Downward J

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Tue Dec 19 00:00:00 EST 2017

Neuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1⁺T_reg cells.

Nature communications

Liu Y,Marin A,Ejlerskov P,Rasmussen LM,Prinz M,Issazadeh-Navikas S

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Western Blot

Mon Apr 24 00:00:00 EDT 2017

Spatiotemporally restricted arenavirus replication induces immune surveillance and type I interferon-dependent tumour regression.

Nature communications

Kalkavan H,Sharma P,Kasper S,Helfrich I,Pandyra AA,Gassa A,Virchow I,Flatz L,Brandenburg T,Namineni S,Heikenwalder M,Höchst B,Knolle PA,Wollmann G,von Laer D,Drexler I,Rathbun J,Cannon PM,Scheu S,Bauer J,Chauhan J,Häussinger D,Willimsky G,Löhning M,Schadendorf D,Brandau S,Schuler M,Lang PA,Lang KS

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Immunofluorescence

Wed Mar 01 00:00:00 EST 2017

Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.

Nature communications

Liu Z,Ravindranathan R,Kalinski P,Guo ZS,Bartlett DL

13-5982 was used in Flow cytometry/Cell sorting to demonstrate in colon and ovarian cancer models that an oncolytic vaccinia virus attracts effector T cells and induces PD-L1 expression on both cancer and immune cells in the tumour.

Mon Mar 27 00:00:00 EDT 2017

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Tue May 17 00:00:00 EDT 2016

Preclinical Development of Ipilimumab and Nivolumab Combination Immunotherapy: Mouse Tumor Models, In Vitro Functional Studies, and Cynomolgus Macaque Toxicology.

PloS one

Selby MJ,Engelhardt JJ,Johnston RJ,Lu LS,Han M,Thudium K,Yao D,Quigley M,Valle J,Wang C,Chen B,Cardarelli PM,Blanset D,Korman AJ

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Immunohistochemistry

Fri Aug 25 00:00:00 EDT 2017

Exploring the induction of preproinsulin-specific Foxp3(+) CD4(+) Treg cells that inhibit CD8(+) T cell-mediated autoimmune diabetes by DNA vaccination.

Scientific reports

Stifter K,Schuster C,Schlosser M,Boehm BO,Schirmbeck R

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Neutralization

Mon Jul 11 00:00:00 EDT 2016

Th2 cell-intrinsic hypo-responsiveness determines susceptibility to helminth infection.

PLoS pathogens

van der Werf N,Redpath SA,Azuma M,Yagita H,Taylor MD

13-5982 was used in Flow cytometry/Cell sorting to demonstrate that during chronic Litomosoides sigmodontis infection, CD4(+) Th2 cells are conditioned towards an intrinsically hypo-responsive phenotype.

Fri Mar 01 00:00:00 EST 2013

IL-10 controls dendritic cell-induced T-cell reactivation in the skin to limit contact hypersensitivity.

The Journal of allergy and clinical immunology

Girard-Madoux MJ,Kel JM,Reizis B,Clausen BE

13-5982 was used in Flow cytometry/Cell sorting to demonstrate that IL-10 signaling in dendritic cells is critical in preventing an exaggerated effector T-cell response in the skin.

Sun Jan 01 00:00:00 EST 2012

Ex vivo expanded hematopoietic stem cells overcome the MHC barrier in allogeneic transplantation.

Cell stem cell

Zheng J,Umikawa M,Zhang S,Huynh H,Silvany R,Chen BP,Chen L,Zhang CC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Fri Aug 05 00:00:00 EDT 2011

Ex vivo expanded hematopoietic stem cells overcome the MHC barrier in allogeneic transplantation.

Cell stem cell

Zheng J,Umikawa M,Zhang S,Huynh H,Silvany R,Chen BP,Chen L,Zhang CC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Fri Aug 05 00:00:00 EDT 2011

Farnesyltransferase inhibitor FTI-277 reduces mortality of septic mice along with improved bacterial clearance.

The Journal of pharmacology and experimental therapeutics

Yang W,Yamada M,Tamura Y,Chang K,Mao J,Zou L,Feng Y,Kida K,Scherrer-Crosbie M,Chao W,Ichinose F,Yu YM,Fischman AJ,Tompkins RG,Yao S,Kaneki M

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 13-5982-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Pre-existing autoimmunity determines type 1 diabetes outcome after Flt3-ligand treatment.

Journal of autoimmunity

Van Belle TL,Juntti T,Liao J,von Herrath MG

13-5982 was used in Flow cytometry/Cell sorting to investigate how Flt3 stimulation actually accelerates autoimmunity when autoreactive CD8 T cells are detectable in blood prior to treatment.

Tue Jun 01 00:00:00 EDT 2010

A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1.

Cell research

Sheng H,Wang Y,Jin Y,Zhang Q,Zhang Y,Wang L,Shen B,Yin S,Liu W,Cui L,Li N

Fri Aug 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1

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Gene Aliases: A530045L16Rik; B7h1; Cd274; Pdcd1l1; Pdcd1lg1; Pdl1

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UniProt ID: (Mouse) Q9EP73

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Entrez Gene ID: (Mouse) 60533

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Function(s)

protein binding immunoglobulin receptor superfamily

Process(es)

immune response signal transduction cell surface receptor signaling pathway positive regulation of cell migration T cell costimulation negative regulation of interferon-gamma production negative regulation of interleukin-10 production positive regulation of T cell proliferation negative regulation of T cell proliferation negative regulation of activated T cell proliferation toxin transport negative regulation of tumor necrosis factor superfamily cytokine production positive regulation of interleukin-10 secretion

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CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Biotin, eBioscience™

CD274 (PD-L1, B7-H1) Antibody (13-5982-82) in Flow

Product Details

13-5982-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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