Invitrogen

CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Super Bright™ 645, eBioscience™

CD274 (PD-L1, B7-H1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 37

CD274 (PD-L1, B7-H1) Antibody (64-5982-82) in Flow

Staining of C57Bl/6 splenocytes with CD3e Monoclonal Antibody, FITC (Product # 11-0031) and 0.06 µg of Rat IgG2a K Isotype Control, Super Bright 645 (Product # 64-4321) (left) or 0.06 µg of Anti-Mouse CD274 (B7-H1) Super Bright 645 (right). Cells in the lymphocyte gate were used for analysis.

CD274 (PD-L1, B7-H1) Antibody in Immunohistochemistry (IHC)

CD274 (PD-L1, B7-H1) Antibody in Neutralization (Neu)

CD274 (PD-L1, B7-H1) Antibody in Functional Assay (Functional)

Product Image Gallery

Product Details

64-5982-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Flow Cytometry (Flow)

0.125 µg/test

View 23 publications 23 publications

Neutralization (Neu)

View 3 publications 3 publications

Functional Assay (Functional)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, lambda

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MIH5

Conjugate

Super Bright™ 645 Super Bright™ 645 Super Bright™ 645

Excitation/Emission Max

414/645 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2688084

Product Specific Information

Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.

Applications Reported: This MIH5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4 degrees C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

AR-A014418 regulates intronic polyadenylation and transcription of PD-L1 through inhibiting CDK12 and CDK13 in tumor cells.

Journal for immunotherapy of cancer

Zhang G,Lan B,Zhang X,Lin M,Liu Y,Chen J,Guo F

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

Sustained release of therapeutic gene by injectable hydrogel for hepatocellular carcinoma.

International journal of pharmaceutics: X

Xu S,Cai J,Cheng H,Wang W

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2023

Contribution of Mesenchymal Stem Cells from Obese Adipose Tissue to PD-L1 Over-Expression and Breast Cancer Progression through Pathogenic Th17 Cell Activation.

Cancers

Blangero F,Robert M,Andraud T,Dumontet C,Vidal H,Eljaafari A

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Mon May 29 00:00:00 EDT 2023

BRAF D594A mutation defines a unique biological and immuno-modulatory subgroup associated with functional CD8⁺ T cell infiltration in colorectal cancer.

Journal of translational medicine

Li W,Zhao C,Li W,Gong Y,Ma K,Lu Y,Liu X,Zhang L,Guo F

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Wed Oct 18 00:00:00 EDT 2023

IFNAR blockade synergizes with oncolytic VSV to prevent virus-mediated PD-L1 expression and promote antitumor T cell activity.

Molecular therapy oncolytics

El-Sayes N,Walsh S,Vito A,Reihani A,Ask K,Wan Y,Mossman K

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Immunohistochemistry

Thu Jun 16 00:00:00 EDT 2022

Modification of PLAC8 by UFM1 affects tumorous proliferation and immune response by impacting PD-L1 levels in triple-negative breast cancer.

Journal for immunotherapy of cancer

Mao M,Chen Y,Yang J,Cheng Y,Xu L,Ji F,Zhou J,Zhang X,Li Z,Chen C,Ju S,Zhang J,Wang L

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Heterogeneity of tyrosine-based melanin anabolism regulates pulmonary and cerebral organotropic colonization microenvironment of melanoma cells.

Theranostics

Wang X,Chen Y,Lan B,Wang Y,Lin W,Jiang X,Ye J,Shang B,Feng C,Liu J,Zhai J,Xu M,Li Q,Lin L,Hu M,Zheng F,Chen L,Shao C,Wang Y,Shi Y

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2022

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

5-Azacytidine-Mediated Modulation of the Immune Microenvironment in Murine Acute Myeloid Leukemia.

Cancers

Ebelt ND,Manuel ER

64-5982-82 was used in Flow cytometry/Cell sorting to find that 5-Aza treatment re-established immune-related transcript expression, suppressed leukemic burden and extended survival in leukemia-challenged mice.

Sun Dec 25 00:00:00 EST 2022

5-Azacytidine-Mediated Modulation of the Immune Microenvironment in Murine Acute Myeloid Leukemia.

Cancers

Ebelt ND,Manuel ER

Sun Dec 25 00:00:00 EST 2022

Scoring model based on the signature of non-m6A-related neoantigen-coding lncRNAs assists in immune microenvironment analysis and TCR-neoantigen pair selection in gliomas.

Journal of translational medicine

Zhao W,Wu Y,Zhao F,Xue Z,Liu W,Cao Z,Zhao Z,Huang B,Han M,Li X

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Immunohistochemistry

Sat Oct 29 00:00:00 EDT 2022

Isotope tracing reveals distinct substrate preference in murine melanoma subtypes with differing anti-tumor immunity.

Cancer & metabolism

Zhang X,Halberstam AA,Zhu W,Leitner BP,Thakral D,Bosenberg MW,Perry RJ

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Multiscale imaging of therapeutic anti-PD-L1 antibody localization using molecularly defined imaging agents.

Journal of nanobiotechnology

Hagemans IM,Wierstra PJ,Steuten K,Molkenboer-Kuenen JDM,van Dalen D,Ter Beest M,van der Schoot JMS,Ilina O,Gotthardt M,Figdor CG,Scheeren FA,Heskamp S,Verdoes M

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Wed Feb 02 00:00:00 EST 2022

Radiation-induced abscopal effect and its enhancement by programmed cell death 1 blockade in the hepatocellular carcinoma: A murine model study.

Clinical and molecular hepatology

Yoo GS,Ahn WG,Kim SY,Kang W,Choi C,Park HC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Oncolytic herpes simplex virus HF10 (canerpaturev) promotes accumulation of CD8⁺ PD-1^- tumor-infiltrating T cells in PD-L1-enriched tumor microenvironment.

International journal of cancer

Eissa IR,Mukoyama N,Abdelmoneim M,Naoe Y,Matsumura S,Bustos-Villalobos I,Ichinose T,Miyajima N,Morimoto D,Tanaka M,Fujimoto Y,Sone M,Kodera Y,Kasuya H

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Preclinical characterization of bemarituzumab, an anti-FGFR2b antibody for the treatment of cancer.

mAbs

Xiang H,Chan AG,Ahene A,Bellovin DI,Deng R,Hsu AW,Jeffry U,Palencia S,Powers J,Zanghi J,Collins H

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Immunocytochemistry

Mon Apr 04 00:00:00 EDT 2022

Collagenase-Expressing Salmonella Targets Major Collagens in Pancreatic Cancer Leading to Reductions in Immunosuppressive Subsets and Tumor Growth.

Cancers

Ebelt ND,Zamloot V,Zuniga E,Passi KB,Sobocinski LJ,Young CA,Blazar BR,Manuel ER

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

Collagenase-Expressing Salmonella Targets Major Collagens in Pancreatic Cancer Leading to Reductions in Immunosuppressive Subsets and Tumor Growth.

Cancers

Ebelt ND,Zamloot V,Zuniga E,Passi KB,Sobocinski LJ,Young CA,Blazar BR,Manuel ER

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

N-cadherin inhibitor creates a microenvironment that protect TILs from immune checkpoints and Treg cells.

Journal for immunotherapy of cancer

Sun Y,Jing J,Xu H,Xu L,Hu H,Tang C,Liu S,Wei Q,Duan R,Guo J,Yang L

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Neutralization

Mon Mar 01 00:00:00 EST 2021

N-cadherin inhibitor creates a microenvironment that protect TILs from immune checkpoints and Treg cells.

Journal for immunotherapy of cancer

Sun Y,Jing J,Xu H,Xu L,Hu H,Tang C,Liu S,Wei Q,Duan R,Guo J,Yang L

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Neutralization

Mon Mar 01 00:00:00 EST 2021

Ketogenic diet and ketone bodies enhance the anticancer effects of PD-1 blockade.

JCI insight

Ferrere G,Tidjani Alou M,Liu P,Goubet AG,Fidelle M,Kepp O,Durand S,Iebba V,Fluckiger A,Daillère R,Thelemaque C,Grajeda-Iglesias C,Alves Costa Silva C,Aprahamian F,Lefevre D,Zhao L,Ryffel B,Colomba E,Arnedos M,Drubay D,Rauber C,Raoult D,Asnicar F,Spector T,Segata N,Derosa L,Kroemer G,Zitvogel L

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Mon Jan 25 00:00:00 EST 2021

Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8⁺ T cell exclusion.

Journal of experimental & clinical cancer research : CR

Fang W,Zhou T,Shi H,Yao M,Zhang D,Qian H,Zeng Q,Wang Y,Jin F,Chai C,Chen T

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Neutralization

Mon Jan 04 00:00:00 EST 2021

USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade.

Nature communications

Yang Z,Xu G,Wang B,Liu Y,Zhang L,Jing T,Tang M,Xu X,Jiao K,Xiang L,Fu Y,Tang D,Zhang X,Jin W,Zhuang G,Zhao X,Liu Y

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Wed Aug 11 00:00:00 EDT 2021

Antigen-Capturing Mesoporous Silica Nanoparticles Enhance the Radiation-Induced Abscopal Effect in Murine Hepatocellular Carcinoma Hepa1-6 Models.

Pharmaceutics

Yang K,Choi C,Cho H,Ahn WG,Kim SY,Shin SW,Kim Y,Jang T,Lee N,Park HC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Downregulation of PD-L1 via amide analogues of brefelamide: Alternatives to antibody-based cancer immunotherapy.

Experimental and therapeutic medicine

Zhang J,Yamada O,Kida S,Murase S,Hattori T,Oshima Y,Kikuchi H

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation.

Journal for immunotherapy of cancer

Wu B,Chiang HC,Sun X,Yuan B,Mitra P,Hu Y,Curiel TJ,Li R

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.

Journal for immunotherapy of cancer

Neuwelt AJ,Kimball AK,Johnson AM,Arnold BW,Bullock BL,Kaspar RE,Kleczko EK,Kwak JW,Wu MH,Heasley LE,Doebele RC,Li HY,Nemenoff RA,Clambey ET

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

CD279 mediates the homeostasis and survival of regulatory T cells by enhancing T cell and macrophage interactions.

FEBS open bio

Liu Y,Gao Y,Hao H,Hou T

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Functional assay

Mon Jun 01 00:00:00 EDT 2020

CD279 mediates the homeostasis and survival of regulatory T cells by enhancing T cell and macrophage interactions.

FEBS open bio

Liu Y,Gao Y,Hao H,Hou T

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Functional assay

Mon Jun 01 00:00:00 EDT 2020

Nuclear imaging-guided PD-L1 blockade therapy increases effectiveness of cancer immunotherapy.

Journal for immunotherapy of cancer

Gao H,Wu Y,Shi J,Zhang X,Liu T,Hu B,Jia B,Wan Y,Liu Z,Wang F

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Restructures the Immune Contexture to Improve Checkpoint Blockade Efficacy.

Biomedicines

Ebelt ND,Zuniga E,Marzagalli M,Zamloot V,Blazar BR,Salgia R,Manuel ER

64-5982 was used in Flow cytometry/Cell sorting to suggest that the success of ICB therapy may be more accurately predicted by taking into account multiple factors such as potential for antigen presentation and immune subset repertoire in addition to markers already being considered.

Wed Dec 16 00:00:00 EST 2020

Epigenetically modulated FOXM1 suppresses dendritic cell maturation in pancreatic cancer and colon cancer.

Molecular oncology

Zhou Z,Chen H,Xie R,Wang H,Li S,Xu Q,Xu N,Cheng Q,Qian Y,Huang R,Shao Z,Xiang M

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

Inhibition of ATM reverses EMT and decreases metastatic potential of cisplatin-resistant lung cancer cells through JAK/STAT3/PD-L1 pathway.

Journal of experimental & clinical cancer research : CR

Shen M,Xu Z,Xu W,Jiang K,Zhang F,Ding Q,Xu Z,Chen Y

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Neutralization

Mon Apr 08 00:00:00 EDT 2019

PD-L1 in tumor microenvironment mediates resistance to oncolytic immunotherapy.

The Journal of clinical investigation

Zamarin D,Ricca JM,Sadekova S,Oseledchyk A,Yu Y,Blumenschein WM,Wong J,Gigoux M,Merghoub T,Wolchok JD

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 64-5982-82) in Flow Cytometry

Mon Apr 02 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1

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Gene Aliases: A530045L16Rik; B7h1; Cd274; Pdcd1l1; Pdcd1lg1; Pdl1

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UniProt ID: (Mouse) Q9EP73

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Entrez Gene ID: (Mouse) 60533

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Function(s)

protein binding immunoglobulin receptor superfamily

Process(es)

immune response signal transduction cell surface receptor signaling pathway positive regulation of cell migration T cell costimulation negative regulation of interferon-gamma production negative regulation of interleukin-10 production positive regulation of T cell proliferation negative regulation of T cell proliferation negative regulation of activated T cell proliferation toxin transport negative regulation of tumor necrosis factor superfamily cytokine production positive regulation of interleukin-10 secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Super Bright™ 645, eBioscience™

CD274 (PD-L1, B7-H1) Antibody (64-5982-82) in Flow

Product Details

64-5982-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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