Invitrogen

CD28 Monoclonal Antibody (37.51), APC, eBioscience™

7 Published Figures

12 References

View all (168) CD28 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (7)

FIGURE: 1 / 8

CD28 Antibody (17-0281-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.25 µg of Golden Syrian Hamster IgG Isotype Control APC (Product # 17-4914-81) (left) or 0.25 µg of Anti-Mouse CD28 APC (right). Total viable cells were used for analysis.

Product Image Gallery

Product Details

17-0281-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 11 publications 11 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Syrian hamster / IgG

Recommended Isotype Control

Syrian Hamster IgG Isotype Control, APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

37.51

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469374

Product Specific Information

Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Microbially-Induced Exosomes from Dendritic Cells Promote Paracrine Immune Senescence: Novel Mechanism of Bone Degenerative Disease in Mice.

Aging and disease

Elsayed R,Elashiry M,Liu Y,Morandini AC,El-Awady A,Elashiry MM,Hamrick M,Cutler CW

17-0281-82 was used in Flow cytometry/Cell sorting to conclude that immune senescence, compounded by advanced age, and accelerated by oral dysbiosis and its induced SASP exosomes, plays a pivotal role in the pathophysiology of experimental periodontitis.

Wed Feb 01 00:00:00 EST 2023

ZhenQi FuZheng formula inhibits the growth of colorectal tumors by modulating intestinal microflora-mediated immune function.

Aging

Meng W,Li Z,Zhang Y,Yang A,Wang Y,Zhou Y,Wu W,Qiu Y,Li L

17-0281-82 was used in Flow cytometry/Cell sorting to provide the experimental basis for the application of ZQFZ as a therapeutic agent for CRC.

Wed Jun 08 00:00:00 EDT 2022

Senescent T Cell Induces Brown Adipose Tissue "Whitening" Via Secreting IFN-γ.

Frontiers in cell and developmental biology

Pan XX,Yao KL,Yang YF,Ge Q,Zhang R,Gao PJ,Ruan CC,Wu F

Published figure using CD28 monoclonal antibody (Product # 17-0281-82) in Flow Cytometry

Tue Mar 23 00:00:00 EDT 2021

VX-765 reduces neuroinflammation after spinal cord injury in mice.

Neural regeneration research

Chen J,Chen YQ,Shi YJ,Ding SQ,Shen L,Wang R,Wang QY,Zha C,Ding H,Hu JG,Lü HZ

Published figure using CD28 monoclonal antibody (Product # 17-0281-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.

Protein & cell

Tong D,Zhang L,Ning F,Xu Y,Hu X,Shi Y

Published figure using CD28 monoclonal antibody (Product # 17-0281-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD28 monoclonal antibody (Product # 17-0281-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

CTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression.

Molecular cell

Ren G,Jin W,Cui K,Rodrigez J,Hu G,Zhang Z,Larson DR,Zhao K

17-0281 was used in Flow cytometry/Cell sorting to study the role of CTCF in regulating cell-to-cell variation of expression.

Thu Sep 21 00:00:00 EDT 2017

CTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression.

Molecular cell

Ren G,Jin W,Cui K,Rodrigez J,Hu G,Zhang Z,Larson DR,Zhao K

17-0281 was used in Flow cytometry/Cell sorting to study the role of CTCF in regulating cell-to-cell variation of expression.

Thu Sep 21 00:00:00 EDT 2017

The ER membrane-anchored ubiquitin ligase Hrd1 is a positive regulator of T-cell immunity.

Nature communications

Xu Y,Zhao F,Qiu Q,Chen K,Wei J,Kong Q,Gao B,Melo-Cardenas J,Zhang B,Zhang J,Song J,Zhang DD,Zhang J,Fan Y,Li H,Fang D

Published figure using CD28 monoclonal antibody (Product # 17-0281-82) in Flow Cytometry

Fri Jul 15 00:00:00 EDT 2016

Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

Shock (Augusta, Ga.)

Shindo Y,Unsinger J,Burnham CA,Green JM,Hotchkiss RS

Published figure using CD28 monoclonal antibody (Product # 17-0281-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Occupancy of lymphocyte LFA-1 by surface-immobilized ICAM-1 is critical for TCR- but not for chemokine-triggered LFA-1 conversion to an open headpiece high-affinity state.

Journal of immunology (Baltimore, Md. : 1950)

Feigelson SW,Pasvolsky R,Cemerski S,Shulman Z,Grabovsky V,Ilani T,Sagiv A,Lemaitre F,Laudanna C,Shaw AS,Alon R

17-0281 was used in Functional assays to study the conformational switches of LFA-1 during both TCR- and chemokine-triggered conversion.

Wed Dec 15 00:00:00 EST 2010

Isolated CD39 expression on CD4+ T cells denotes both regulatory and memory populations.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Zhou Q,Yan J,Putheti P,Wu Y,Sun X,Toxavidis V,Tigges J,Kassam N,Enjyoji K,Robson SC,Strom TB,Gao W

17-0281 was used in Flow cytometry/Cell sorting to study how besides Tregs, CD39 is also expressed on pre-existing memory T cells of Th1-, Th2- and Th17-types with heightened alloreactivity.

Thu Oct 01 00:00:00 EDT 2009

Light chain editing generates polyreactive antibodies in chronic graft-versus-host reaction.

The Journal of experimental medicine

Witsch EJ,Cao H,Fukuyama H,Weigert M

17-0281 was used in Flow cytometry/Cell sorting to investigate the structure, specificity, and origin of the induced autoantibodies in chronic graft-versus-host disease, showing that light chain editing generates polyreactive antibodies.

Mon Jul 10 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: CD28; MGC138290; sCD28; soluble CD28; T-cell-specific surface glycoprotein CD28; T44

View more View less

Gene Aliases: Cd28

View more View less

UniProt ID: (Mouse) P31041

View more View less

Entrez Gene ID: (Mouse) 12487

View more View less

Function(s)

protease binding SH3/SH2 adaptor activity protein homodimerization activity immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD28 Monoclonal Antibody (37.51), APC, eBioscience™

CD28 Antibody (17-0281-82) in Flow

Product Details

17-0281-82

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!