Invitrogen

CD282 (TLR2) Monoclonal Antibody (6C2), FITC, eBioscience™

11 Published Figures

21 References

View all (76) TLR2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (11)

CD282 (TLR2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 12

CD282 (TLR2) Antibody (11-9021-82) in Flow

BALB/c splenocytes were pre-incubated with Anti-Mouse CD16/CD32 Purified (Product # 14-0161-82) and then stained with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.5 µg of Rat IgG2b kappa Isotype Control FITC (Product # 11-4031-82) (left) or 0.5 µg of Anti-Mouse CD282 (TLR2) FITC (right). Total viable cells were used for analysis.

CD282 (TLR2) Antibody in Immunocytochemistry (ICC/IF)

CD282 (TLR2) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

11-9021-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

6C2

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465440

Product Specific Information

Description: The 6C2 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2). mouse TLR2 is expressed by the myeloid lineage, including macrophage and dendritic cells in splenocytes and the RAW264.7 cell line. To date, at least twelve members of the Toll family have been identified in human and mouse. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed on the surface of cells and is responsible for distinguishing different pathogens.

Applications Reported: 6C2 has been reported for use in flow cytometric analysis. For optimal flow cytometry staining with this antibody, it is recommended that a directly conjugated 6C2 (FITC, PE, or Biotin) be used in combination with FcR blocking with anti-mouse CD16/CD32 (clone 93, Product # 14-0161).

Applications Tested: The 6C2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Glial cell line-derived neurotrophic factor increases matrix metallopeptidase 9 and 14 expression in microglia and promotes microglia-mediated glioma progression.

Journal of neuroscience research

Huang Y,Zhang B,Haneke H,Haage V,Lubas M,Yuan Y,Xia P,Motta E,Nanvuma C,Dzaye O,Hu F,Kettenmann H

11-9021-82 was used in Flow Cytometry to provide evidence that glial cell line-derived neurotrophic factor is capable to induce microglial MMP9/14 production and thus promotes glioma growth.

Thu Apr 01 00:00:00 EDT 2021

Glial cell line-derived neurotrophic factor increases matrix metallopeptidase 9 and 14 expression in microglia and promotes microglia-mediated glioma progression.

Journal of neuroscience research

Huang Y,Zhang B,Haneke H,Haage V,Lubas M,Yuan Y,Xia P,Motta E,Nanvuma C,Dzaye O,Hu F,Kettenmann H

11-9021-82 was used in Flow Cytometry to provide evidence that glial cell line-derived neurotrophic factor is capable to induce microglial MMP9/14 production and thus promotes glioma growth.

Thu Apr 01 00:00:00 EDT 2021

Macrophage mediated recognition and clearance of Borrelia burgdorferi elicits MyD88-dependent and -independent phagosomal signals that contribute to phagocytosis and inflammation.

BMC immunology

Benjamin SJ,Hawley KL,Vera-Licona P,La Vake CJ,Cervantes JL,Ruan Y,Radolf JD,Salazar JC

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9021-82) in Immunocytochemistry

Mon May 17 00:00:00 EDT 2021

Spatial organization of FcγR and TLR2/1 on phagosome membranes differentially regulates their synergistic and inhibitory receptor crosstalk.

Scientific reports

Li W,Li M,Anthony SM,Yu Y

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9021-82) in Immunocytochemistry

Mon Jun 28 00:00:00 EDT 2021

Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.

Nature communications

Ramakrishna C,Kujawski M,Chu H,Li L,Mazmanian SK,Cantin EM

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9021-82) in Flow Cytometry

Tue May 14 00:00:00 EDT 2019

Immunology

Xu F,Zhang C,Zou Z,Fan EKY,Chen L,Li Y,Billiar TR,Wilson MA,Shi X,Fan J

11-9021 was used in Flow cytometry/Cell sorting to suggest an important role of Atg5 and autophagy in maintaining the function of neutrophil extracellular trap formation.

Tue Aug 01 00:00:00 EDT 2017

Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal Gamma-Delta T Cells.

Frontiers in pharmacology

Li M,Gao J,Tang Y,Liu M,Wu S,Qu K,Long X,Li H,Liu M,Liu Y,Yuan J,Mao L,Liu Y,Zheng X,Wang E,Wang J,Yang Y

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9021-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

CD14 is critical for TLR2-mediated M1 macrophage activation triggered by N-glycan recognition.

Scientific reports

da Silva TA,Zorzetto-Fernandes ALV,Cecílio NT,Sardinha-Silva A,Fernandes FF,Roque-Barreira MC

11-9021 was used in Flow cytometry/Cell sorting to show that CD14 is critical for ArtinM-induced macrophage activation in mice.

Tue Aug 01 00:00:00 EDT 2017

Toll-Like receptor 2 (TLR2) and TLR9 play opposing roles in host innate immunity against Salmonella enterica serovar Typhimurium infection.

Infection and immunity

Zhan R,Han Q,Zhang C,Tian Z,Zhang J

11-9021 was used in Flow cytometry/Cell sorting to investigate the roles of TLR2 and TLR9 in regulating innate immunity against oral infection of Salmonella enterica serovar Typhimurium in mice.

Wed Apr 01 00:00:00 EDT 2015

Chronic morphine and HIV-1 Tat promote differential central nervous system trafficking of CD3+ and Ly6C+ immune cells in a murine Streptococcus pneumoniae infection model.

Journal of neuroinflammation

Dutta R,Roy S

11-9021 was used in Flow cytometry/Cell sorting to explore the role of systemic infection with Streptococcus pneumoniae in the recruitment of peripheral leukocytes into the central nervous system.

Sat Jun 20 00:00:00 EDT 2015

Outer membrane protein A (OmpA) of Shigella flexneri 2a induces TLR2-mediated activation of B cells: involvement of protein tyrosine kinase, ERK and NF-κB.

PloS one

Bhowmick R,Pore D,Chakrabarti MK

11-9021 was used in Flow cytometry/Cell sorting to investigate whether OmpA can modulate B cell functions.

Wed Jun 10 00:00:00 EDT 2015

Eosinophil contamination of thioglycollate-elicited peritoneal macrophage cultures skews the functional readouts of in vitro assays.

Journal of leukocyte biology

Misharin AV,Saber R,Perlman H

11-9021 was used in Flow cytometry/Cell sorting to examine the presence of other cell types in freshly isolated and cultured thioglycollate-elicited peritoneal cells.

Wed Aug 01 00:00:00 EDT 2012

Protective role for TLR4 signaling in atherosclerosis progression as revealed by infection with a common oral pathogen.

Journal of immunology (Baltimore, Md. : 1950)

Hayashi C,Papadopoulos G,Gudino CV,Weinberg EO,Barth KR,Madrigal AG,Chen Y,Ning H,LaValley M,Gibson FC,Hamilton JA,Genco CA

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9021-82) in Immunohistochemistry

Mon Oct 01 00:00:00 EDT 2012

The tumor suppressor ARF regulates innate immune responses in mice.

Journal of immunology (Baltimore, Md. : 1950)

Través PG,López-Fontal R,Luque A,Hortelano S

11-9021 was used in Flow cytometry/Cell sorting to investigate the role of the tumour suppressor alternative reading frame (ARF) in the regulation of innate immunity in mice.

Thu Dec 15 00:00:00 EST 2011

Role for MyD88, TLR2 and TLR9 but not TLR1, TLR4 or TLR6 in experimental autoimmune encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Miranda-Hernandez S,Gerlach N,Fletcher JM,Biros E,Mack M,Körner H,Baxter AG

11-9021 was used in Flow cytometry/Cell sorting to examine the effects of targeted deletions of specific TLRs on the induction of toxin-induced autoimmune encephalomyelitis.

Fri Jul 15 00:00:00 EDT 2011

TLR2 hypersensitivity of astrocytes as functional consequence of previous inflammatory episodes.

Journal of immunology (Baltimore, Md. : 1950)

Henn A,Kirner S,Leist M

11-9021 was used in Flow cytometry/Cell sorting to demonstrate that astrocytes exposed to activated microglia assume a different functional phenotype involving longer term TLR2 responsiveness.

Tue Mar 01 00:00:00 EST 2011

Toll-like receptor 2- and MyD88-dependent phosphatidylinositol 3-kinase and Rac1 activation facilitates the phagocytosis of Listeria monocytogenes by murine macrophages.

Infection and immunity

Shen Y,Kawamura I,Nomura T,Tsuchiya K,Hara H,Dewamitta SR,Sakai S,Qu H,Daim S,Yamamoto T,Mitsuyama M

11-9021 was used in Flow cytometry/Cell sorting to investigate receptor activation facilitating the phagocytosis of Listeria monocytogenes.

Tue Jun 01 00:00:00 EDT 2010

Increased endothelial expression of Toll-like receptor 2 at sites of disturbed blood flow exacerbates early atherogenic events.

The Journal of experimental medicine

Mullick AE,Soldau K,Kiosses WB,Bell TA,Tobias PS,Curtiss LK

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9021-82) in Immunofluorescence

Mon Feb 18 00:00:00 EST 2008

Toll-like receptor 2 plays a critical role in the progression of atherosclerosis that is independent of dietary lipids.

Atherosclerosis

Liu X,Ukai T,Yumoto H,Davey M,Goswami S,Gibson FC,Genco CA

11-9021 was used in Flow cytometry/Cell sorting to demonstrate that TLR2 plays a critical role in the progression of atherosclerosis in Apoe-/- mice.

Tue Jan 01 00:00:00 EST 2008

Macrophage-elicited osteoclastogenesis in response to bacterial stimulation requires Toll-like receptor 2-dependent tumor necrosis factor-alpha production.

Infection and immunity

Ukai T,Yumoto H,Gibson FC,Genco CA

11-9021 was used in Flow cytometry/Cell sorting to define a requirement for TLR2 in TNF-alpha-elicited osteoclastogenesis in response to exposure to P. gingivalis.

Fri Feb 01 00:00:00 EST 2008

Escherichia coli strain Nissle 1917 ameliorates experimental colitis via toll-like receptor 2- and toll-like receptor 4-dependent pathways.

Infection and immunity

Grabig A,Paclik D,Guzy C,Dankof A,Baumgart DC,Erckenbrecht J,Raupach B,Sonnenborn U,Eckert J,Schumann RR,Wiedenmann B,Dignass AU,Sturm A

11-9021 was used in Flow cytometry/Cell sorting to analyse the effects of probiotic Escherichia coli Nissle strain 1917 in experimental colitis, showing that it ameliorates experimental colitis via TLR2- and TLR4-dependent pathways.

Sat Jul 01 00:00:00 EDT 2006

TLR2 modulates inflammation in zymosan-induced arthritis in mice.

Arthritis research & therapy

Frasnelli ME,Tarussio D,Chobaz-Péclat V,Busso N,So A

Published figure using CD282 (TLR2) monoclonal antibody (Product # 11-9021-82) in Immunohistochemistry

Thu Dec 15 00:00:00 EST 2005

Bioinformatics

Protein Aliases: CD282; TLR-2; Toll-like receptor 2

View more View less

Gene Aliases: Ly105; Tlr2

View more View less

UniProt ID: (Mouse) Q9QUN7

View more View less

Entrez Gene ID: (Mouse) 24088

View more View less

Function(s)

receptor activity transmembrane signaling receptor activity protein binding signaling pattern recognition receptor activity triacyl lipopeptide binding diacyl lipopeptide binding peptidoglycan binding protein heterodimerization activity lipoteichoic acid binding lipopeptide binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD282 (TLR2) Monoclonal Antibody (6C2), FITC, eBioscience™

CD282 (TLR2) Antibody (11-9021-82) in Flow

Product Details

11-9021-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!