Invitrogen

CD289 (TLR9) Monoclonal Antibody (M9.D6), FITC, eBioscience™

CD289 (TLR9) Antibody in Flow Cytometry (Flow)

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CD289 (TLR9) Antibody (11-9093-82) in Flow

Non-transfected (left) and mouse TLR9-transfected (right) 293T cells were intracellularly stained with 0.25 µg of Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-80) (open histogram) or 0.5 µg of Anti-Mouse CD289 (TLR9) FITC (filled histogram). Total viable cells were used for analysis.

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Product Details

11-9093-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M9.D6

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572523

Product Specific Information

Description: M9.D6 is generated against a peptide derived from the extracellular portion of mouse TLR9. Predominantly expressed as an intracellular protein, TLR9 is a ~115-120 kDa molecule which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9+ B cells. When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on RBC-lysed mouse splenocytes stained for intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Further studies are needed to determine the relationship between mRNA expression and protein detection.

Applications Reported: This M9.D6 antibody has been reported for use in intracellular flow cytometric analysis.

Applications Tested: This M9.D6 antibody has been tested by intracellular staining and flow cytometric analysis of fixed and permeabilized mouse TLR9 transfected 293T cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Hepatic DNA deposition drives drug-induced liver injury and inflammation in mice.

Hepatology (Baltimore, Md.)

Marques PE,Oliveira AG,Pereira RV,David BA,Gomides LF,Saraiva AM,Pires DA,Novaes JT,Patricio DO,Cisalpino D,Menezes-Garcia Z,Leevy WM,Chapman SE,Mahecha G,Marques RE,Guabiraba R,Martins VP,Souza DG,Mansur DS,Teixeira MM,Leite MF,Menezes GB

11-9093 was used in Flow cytometry/Cell sorting to examine whether DNA released from damaged hepatocytes accumulates into necrotic liver, and study the impact of its recognition by the immune system.

Thu Jan 01 00:00:00 EST 2015

Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells.

Frontiers in immunology

Vogel AJ,Brown DM

11-9093 was used in Flow cytometry/Cell sorting to exhibit a novel vaccine platform for inducing robust T cell responses capable of protecting against heterologous influenza A challenges.

Fri Jul 10 00:00:00 EDT 2015

Toll-Like receptor 2 (TLR2) and TLR9 play opposing roles in host innate immunity against Salmonella enterica serovar Typhimurium infection.

Infection and immunity

Zhan R,Han Q,Zhang C,Tian Z,Zhang J

11-9093 was used in Flow cytometry/Cell sorting to investigate the roles of TLR2 and TLR9 in regulating innate immunity against oral infection of Salmonella enterica serovar Typhimurium in mice.

Wed Apr 01 00:00:00 EDT 2015

Cytokine-driven loss of plasmacytoid dendritic cell function in chronic lymphocytic leukemia.

Leukemia

Saulep-Easton D,Vincent FB,Le Page M,Wei A,Ting SB,Croce CM,Tam C,Mackay F

11-9093 was used in Flow cytometry/Cell sorting to demonstrate that plasmacytoid dendritic cells are reduced in number and functionally impaired in progressive chronic lymphocytic leukemia.

Wed Oct 01 00:00:00 EDT 2014

A dynamic flux in natural killer cell subsets as a function of the duration of alcohol ingestion.

Alcoholism, clinical and experimental research

Ballas ZK,Cook RT,Shey MR,Coleman RA

11-9093 was used in Flow cytometry/Cell sorting to investigate the effect of duration of alcohol ingestion on NK cell function, showing a dynamic flux in NK cell subsets as a function of ingestion.

Tue May 01 00:00:00 EDT 2012

Mechanisms by which chronic ethanol feeding limits the ability of dendritic cells to stimulate T-cell proliferation.

Alcoholism, clinical and experimental research

Fan J,Edsen-Moore MR,Turner LE,Cook RT,Legge KL,Waldschmidt TJ,Schlueter AJ

11-9093 was used in Flow cytometry/Cell sorting to assess the functional capability of ethanol-exposed dendritic cells, using a murine model of chronic alcohol abuse.

Sat Jan 01 00:00:00 EST 2011

Production of autoantibodies by murine B-1a cells stimulated with Helicobacter pylori urease through toll-like receptor 2 signaling.

Infection and immunity

Kobayashi F,Watanabe E,Nakagawa Y,Yamanishi S,Norose Y,Fukunaga Y,Takahashi H

11-9093 was used in Flow cytometry/Cell sorting to determine the role of functional urease expression in the production of various autoantibodies and the induction of autoimmune disorders.

Thu Dec 01 00:00:00 EST 2011

The myeloid differentiation factor 88 (MyD88) is required for CD4+ T cell effector function in a murine model of inflammatory bowel disease.

Journal of immunology (Baltimore, Md. : 1950)

Fukata M,Breglio K,Chen A,Vamadevan AS,Goo T,Hsu D,Conduah D,Xu R,Abreu MT

Fri Feb 01 00:00:00 EST 2008

Role of scavenger receptor MARCO in macrophage responses to CpG oligodeoxynucleotides.

Journal of leukocyte biology

Józefowski S,Sulahian TH,Arredouani M,Kobzik L

11-9093 was used in Flow cytometry/Cell sorting to study the role of scavenger receptor MARCO in macrophage responses to CpG oligodeoxynucleotides.

Sun Oct 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: CD289; Toll-like receptor 9

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Gene Aliases: Tlr9

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UniProt ID: (Mouse) Q9EQU3

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Entrez Gene ID: (Mouse) 81897

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Function(s)

transmembrane signaling receptor activity interleukin-1 receptor binding protein binding siRNA binding unmethylated CpG binding

Process(es)

Performance Guarantee

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CD289 (TLR9) Monoclonal Antibody (M9.D6), FITC, eBioscience™

CD289 (TLR9) Antibody (11-9093-82) in Flow

Product Details

11-9093-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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