Invitrogen

CD31 Monoclonal Antibody (TLD-3A12)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

12 References

View all (297) CD31 (PECAM-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD31 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

CD31 Antibody (MA1-80069) in ICC/IF

Immunofluorescence analysis of Platelet endothelial cell adhesion molecule was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD31 Monoclonal Antibody (TLD-3A12) (Product # MA1-80069) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondar... View More

CD31 Antibody in Immunohistochemistry (IHC)

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CD31 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of Platelet endothelial cell adhesion molecule was observed to be high in HUVEC in comparison to no expression in MDA-MB-231 using Anti-CD31 Monoclonal Antibody (TLD-3A12) (Product # MA1-80069) in Western Blot (Ref :https://www.nature.com/articles/onc2013214). {RE}

Product Details

MA1-80069

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

1:10-1:100

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Affinity Purification (AP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Rhesus monkey, Rat

Published species

Hamster, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TLD-3A12

Immunogen

Activated, Lewis rat derived microglial cells.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_928130

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Regulated contribution of local and systemic immunity to new bone regeneration by modulating B/Sr concentration of bioactive borosilicate glass.

Materials today. Bio

Ding J,Zhao J,Wang L,Chen X,Jiang D,Qin M,Zhu Z,Wang D,Jia W

MA1-80069 was used in Immunohistochemistry to provide a new approach and strategy in optimizing complex composition of novel BG and sheds light on the importance of spleen through modulating systemic immune response to contribute to local bone regeneration.

Sat Apr 01 00:00:00 EDT 2023

Effects of a neurokinin-1 receptor antagonist in the acute phase after thoracic spinal cord injury in a rat model.

Frontiers in molecular neuroscience

Zheng G,Harms AK,Tail M,Zhang H,Nimmo A,Skutella T,Kiening K,Unterberg A,Zweckberger K,Younsi A

MA1-80069 was used in Immunohistochemistry to observe the effect of antagonizing the binding of the neuropeptide Substance-P (SP) to its neurokinin-1 (NK1) receptor in a rodent spinal cord injury (SCI) model.

Wed May 31 00:00:00 EDT 2023

Propofol suppresses adipose-derived stem cell progression via PI3K/AKT-Wnt signaling pathway.

BMC anesthesiology

Yin G,Wang J,Zhong Y,Wu W

MA1-80069 was used in Flow Cytometry to indicate that the PI3K/AKT-Wnt pathways mediated propofol-inhibited ADSC proliferation, providing new insights into the propofol application in ADSCs.

Wed Mar 09 00:00:00 EST 2022

Propofol suppresses adipose-derived stem cell progression via PI3K/AKT-Wnt signaling pathway.

BMC anesthesiology

Yin G,Wang J,Zhong Y,Wu W

MA1-80069 was used in Flow Cytometry to indicate that the PI3K/AKT-Wnt pathways mediated propofol-inhibited ADSC proliferation, providing new insights into the propofol application in ADSCs.

Wed Mar 09 00:00:00 EST 2022

Mitomycin C induces pulmonary vascular endothelial-to-mesenchymal transition and pulmonary veno-occlusive disease via Smad3-dependent pathway in rats.

British journal of pharmacology

Zhang C,Lu W,Luo X,Liu S,Li Y,Zheng Q,Liu W,Wu X,Chen Y,Jiang Q,Zhang Z,Gu G,Chen J,Chen H,Liao J,Liu C,Hong C,Tang H,Sun D,Yang K,Wang J

MA1-80069 was used in Cell Culture to indicate that targeted inhibition of Smad3 leads to a potential, novel strategy for PVOD therapy, likely by inhibiting the EndoMT in pulmonary microvasculature.

Fri Jan 01 00:00:00 EST 2021

Regenerative Effects of Hypoxia Primed Flowable Placental Formulation in Muscle and Dermal Injury.

International journal of molecular sciences

Dhall S,Park MS,Li C,Sathyamoorthy M

Published figure using CD31 monoclonal antibody (Product # MA1-80069) in Immunocytochemistry

Thu Jul 01 00:00:00 EDT 2021

Regenerative Effects of Hypoxia Primed Flowable Placental Formulation in Muscle and Dermal Injury.

International journal of molecular sciences

Dhall S,Park MS,Li C,Sathyamoorthy M

Published figure using CD31 monoclonal antibody (Product # MA1-80069) in Immunocytochemistry

Thu Jul 01 00:00:00 EDT 2021

Dysregulation of BMP9/BMPR2/SMAD signalling pathway contributes to pulmonary fibrosis and pulmonary hypertension induced by bleomycin in rats.

British journal of pharmacology

Jiang Q,Liu C,Liu S,Lu W,Li Y,Luo X,Ma R,Zhang C,Chen H,Chen Y,Zhang Z,Hong C,Guo W,Wang T,Yang K,Wang J

MA1-80069 was used in Affinity Purification to show that therapeutic approaches reinforcing BMP9/BMPR2/SMAD signalling might be ideal strategies for this subtype of pulmonary hypertension.

Fri Jan 01 00:00:00 EST 2021

YangXue QingNao Wan, a Compound Chinese Medicine, Attenuates Cerebrovascular Hyperpermeability and Neuron Injury in Spontaneously Hypertensive Rat: Effect and Mechanism.

Frontiers in physiology

Jiao YQ,Huang P,Yan L,Sun K,Pan CS,Li Q,Fan JY,Ma ZZ,Han JY

MA1-80069 was used in Immunohistochemistry to explore the effect of YangXue QingNao Wan, a compound Chinese medicine, on cerebrovascular hyperpermeability, neuronal injury, and related mechanisms in spontaneously hypertensive rat.

Sun Apr 10 00:00:00 EDT 2022

YangXue QingNao Wan and Silibinin Capsules, the Two Chinese Medicines, Attenuate Cognitive Impairment in Aged LDLR (+/-) Golden Syrian Hamsters Involving Protection of Blood Brain Barrier.

Frontiers in physiology

Gu YY,Huang P,Li Q,Liu YY,Liu G,Wang YH,Yi M,Yan L,Wei XH,Yang L,Hu BH,Zhao XR,Chang X,Sun K,Pan CS,Cui YC,Chen QF,Wang CS,Fan JY,Ma ZZ,Han JY

MA1-80069 was used in Immunohistochemistry to explore the effect and the underlying mechanism of YangXue QingNao Wan (YXQNW) and Silibinin Capsules (SC), the two Chinese medicines, on cognitive impairment in older people with familial hyperlipidaemia.

Wed Sep 30 00:00:00 EDT 2020

YangXue QingNao Wan and Silibinin Capsules, the Two Chinese Medicines, Attenuate Cognitive Impairment in Aged LDLR (+/-) Golden Syrian Hamsters Involving Protection of Blood Brain Barrier.

Frontiers in physiology

Gu YY,Huang P,Li Q,Liu YY,Liu G,Wang YH,Yi M,Yan L,Wei XH,Yang L,Hu BH,Zhao XR,Chang X,Sun K,Pan CS,Cui YC,Chen QF,Wang CS,Fan JY,Ma ZZ,Han JY

Wed Sep 30 00:00:00 EDT 2020

Bushen Huoxue Attenuates Diabetes-Induced Cognitive Impairment by Improvement of Cerebral Microcirculation: Involvement of RhoA/ROCK/moesin and Src Signaling Pathways.

Frontiers in physiology

Li Y,Li Q,Pan CS,Yan L,Hu BH,Liu YY,Yang L,Huang P,Zhao SY,Wang CS,Fan JY,Wang XM,Han JY

MA1-80069 was used in Immunohistochemistry to investigate the mechanisms of Bushen Huoxue (BSHX) in treating diabetes-induced cognitive impairment in female KKAy mice through western blotting.

Thu Oct 01 00:00:00 EDT 2020

Bushen Huoxue Attenuates Diabetes-Induced Cognitive Impairment by Improvement of Cerebral Microcirculation: Involvement of RhoA/ROCK/moesin and Src Signaling Pathways.

Frontiers in physiology

Li Y,Li Q,Pan CS,Yan L,Hu BH,Liu YY,Yang L,Huang P,Zhao SY,Wang CS,Fan JY,Wang XM,Han JY

MA1-80069 was used in Immunohistochemistry to investigate the mechanisms of Bushen Huoxue (BSHX) in treating diabetes-induced cognitive impairment in female KKAy mice through western blotting.

Thu Oct 01 00:00:00 EDT 2020

Inhibitor of differentiation 4 (ID4) inactivation promotes de novo steroidogenesis and castration-resistant prostate cancer.

Molecular endocrinology (Baltimore, Md.)

Patel D,Knowell AE,Korang-Yeboah M,Sharma P,Joshi J,Glymph S,Chinaranagari S,Nagappan P,Palaniappan R,Bowen NJ,Chaudhary J

Published figure using CD31 monoclonal antibody (Product # MA1-80069) in Immunohistochemistry

Fri Aug 01 00:00:00 EDT 2014

Long-Term Stimulation with Electroacupuncture at DU20 and ST36 Rescues Hippocampal Neuron through Attenuating Cerebral Blood Flow in Spontaneously Hypertensive Rats.

Evidence-based complementary and alternative medicine : eCAM

Tian GH,Sun K,Huang P,Zhou CM,Yao HJ,Huo ZJ,Hao HF,Yang L,Pan CS,He K,Fan JY,Li ZG,Han JY

MA1-80069 was used in immunohistochemistry to study the mechanisms underlying the beneficial effects of electroacupuncture at DU20 and ST36 on cerebral blood flow, learning and memory in spontaneously hypertensive rats

Mon May 13 00:00:00 EDT 2013

Erythropoietin protects against doxorubicin-induced heart failure.

American journal of physiology. Heart and circulatory physiology

Ammar HI,Saba S,Ammar RI,Elsayed LA,Ghaly WB,Dhingra S

MA1-80069 was used in immunohistochemistry to study the ability of erythropoeitin to protect against heart failure induced by treatment with the cancer drug doxorubicin

Thu Dec 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: CD31; PECAM-1; Platelet endothelial cell adhesion molecule

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Gene Aliases: CD31; Pecam; Pecam1

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UniProt ID: (Rat) Q3SWT0

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Entrez Gene ID: (Rhesus monkey) 718302, (Rat) 29583

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Function(s)

protein binding immunoglobulin receptor superfamily

Process(es)

positive regulation of leukocyte migration phagocytosis cell adhesion Rho protein signal transduction regulation of cell migration negative regulation of actin filament polymerization response to cytokine negative regulation of GTPase activity wound healing positive regulation of tyrosine phosphorylation of Stat5 protein negative regulation of peptidyl-tyrosine phosphorylation diapedesis glomerular endothelium development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD31 Monoclonal Antibody (TLD-3A12)

CD31 Antibody (MA1-80069) in ICC/IF

Product Details

MA1-80069

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Affinity Purification (AP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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