Invitrogen

CD366 (TIM3) Monoclonal Antibody (F38-2E2), PE, eBioscience™

CD366 (TIM3) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD366 (TIM3) Antibody (12-3109-42) in Flow

Staining of 3-day Anti-Human CD3 and CD28 Functional Grade Purifieds (Product # 16-0039-81 and Product # 16-0289-81)-stimulated normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0049-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD366 (TIM3) PE (right). Total viable cells, as determined by Fixable Viability Dye eFluor® 660 (Product # 65-0864-14), were used for analysis.

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Product Details

12-3109-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

F38-2E2

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572605

Product Specific Information

Description: This F38-2E2 monoclonal antibody reacts with human CD366, also known as T cell immunoglobulin and mucin domain-containing protein 3 (TIM3) or HAVCR2. This cell surface receptor is expressed on activated CD4+ T cell subsets (e.g. Th1, Th17, and Treg), CD8+ T cells, monocytes, dendritic cells, and mast cells. Due to alternative splicing, CD366 exists as membrane-bound and soluble forms. Galectin-9 has been identified as the ligand for CD366. In humans, this receptor negatively regulates CD4+ T cells, influencing the secretion of some Th1- and Th17-related cytokines. CD366 has also been implicated in tolerance, autoimmune disease (e.g. multiple sclerosis), and HIV infection.

Applications Reported: This F38-2E2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This F38-2E2 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

MYEF2: an immune infiltration-related prognostic factor in IDH-wild-type glioblastoma.

Aging

Zhang Y,Wen Y,Nie J,Wang T,Wang G,Gao Q,Cao Y,Wang H,Qi S,Xie S

Published figure using CD366 (TIM3) monoclonal antibody (Product # 12-3109-42) in Flow Cytometry

Tue Aug 08 00:00:00 EDT 2023

High-dimensional immune cell profiling of cerebrospinal fluid from patients with metastatic breast cancer and leptomeningeal disease.

NPJ breast cancer

Im KW,Huppert LA,Malevanchik L,Rugo HS,Combes AJ,Campbell MJ,Krummel MF,Melisko ME

12-3109-42 was used in Flow cytometry/Cell sorting to suggest that patients with LMD may have lower overall immune infiltrates than patients without LMD, suggesting a more permissive CSF immune microenvironment but a higher frequency of partially exhausted CD8+ T cells, which may offer an important therapeutic target.

Fri Apr 07 00:00:00 EDT 2023

Is IFN expression by NK cells a hallmark of severe COVID-19?

Cytokine

Csordas BG,de Sousa Palmeira PH,Peixoto RF,Comberlang FCQDDS,de Medeiros IA,Azevedo FLAA,Veras RC,Janebro DI,Amaral IPG,Barbosa-Filho JM,Keesen TSL

12-3109-42 was used in Flow cytometry/Cell sorting to demonstrate that CD3-CD56+ NK cells frequency in the volunteers who recovered from mild COVID-19 (Mild CoV) presented a significant increase compared to the healthy control (HC) and individuals recovering from severe COVID-19 (Severe CoV) groups.

Thu Sep 01 00:00:00 EDT 2022

TIM-3 Expression Level on AML Blasts Correlates With Presence of Core Binding Factor Translocations Rather Than Clinical Outcomes.

Frontiers in oncology

Hong J,Xia L,Huang Z,Yuan X,Liang X,Dai J,Wu Z,Liang L,Ruan M,Long Z,Cheng X,Chen X,Ni J,Ge J,Li Q,Zeng Q,Xia R,Wang Y,Yang M

12-3109-42 was used in Flow cytometry/Cell sorting to investigate the expression of T-cell immunoglobulin and mucin domain-containing molecule 3 (TIM-3) in acute myeloid leukemia (AML) patients.

Sat Jul 16 00:00:00 EDT 2022

Humans with inherited T cell CD28 deficiency are susceptible to skin papillomaviruses but are otherwise healthy.

Cell

Béziat V,Rapaport F,Hu J,Titeux M,Bonnet des Claustres M,Bourgey M,Griffin H,Bandet É,Ma CS,Sherkat R,Rokni-Zadeh H,Louis DM,Changi-Ashtiani M,Delmonte OM,Fukushima T,Habib T,Guennoun A,Khan T,Bender N,Rahman M,About F,Yang R,Rao G,Rouzaud C,Li J,Shearer D,Balogh K,Al Ali F,Ata M,Dabiri S,Momenilandi M,Nammour J,Alyanakian MA,Leruez-Ville M,Guenat D,Materna M,Marcot L,Vladikine N,Soret C,Vahidnezhad H,Youssefian L,Saeidian AH,Uitto J,Catherinot É,Navabi SS,Zarhrate M,Woodley DT,Jeljeli M,Abraham T,Belkaya S,Lorenzo L,Rosain J,Bayat M,Lanternier F,Lortholary O,Zakavi F,Gros P,Orth G,Abel L,Prétet JL,Fraitag S,Jouanguy E,Davis MM,Tangye SG,Notarangelo LD,Marr N,Waterboer T,Langlais D,Doorbar J,Hovnanian A,Christensen N,Bossuyt X,Shahrooei M,Casanova JL

12-3109-42 was used in Flow cytometry/Cell sorting to conclude that human CD28-dependent T cell responses are largely redundant for protective immunity.

Thu Jul 08 00:00:00 EDT 2021

TIGIT⁺ TIM-3⁺ NK cells are correlated with NK cell exhaustion and disease progression in patients with hepatitis B virus‑related hepatocellular carcinoma.

Oncoimmunology

Yu L,Liu X,Wang X,Yan F,Wang P,Jiang Y,Du J,Yang Z

Published figure using CD366 (TIM3) monoclonal antibody (Product # 12-3109-42) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

SLC1A1 mediated glutamine addiction and contributed to natural killer T-cell lymphoma progression with immunotherapeutic potential.

EBioMedicine

Xiong J,Wang N,Zhong HJ,Cui BW,Cheng S,Sun R,Chen JY,Xu PP,Cai G,Wang L,Sun XJ,Huang JY,Zhao WL

Published figure using CD366 (TIM3) monoclonal antibody (Product # 12-3109-42) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation.

Frontiers in immunology

Cerqueira OLD,Clavijo-Salomon MA,Cardoso EC,Citrangulo Tortelli Junior T,Mendonça SA,Barbuto JAM,Strauss BE

Published figure using CD366 (TIM3) monoclonal antibody (Product # 12-3109-42) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

YY1 Upregulates Checkpoint Receptors and Downregulates Type I Cytokines in Exhausted, Chronically Stimulated Human T Cells.

iScience

Balkhi MY,Wittmann G,Xiong F,Junghans RP

12-3109 was used in Flow cytometry/Cell sorting to elucidate a Yin Yang-1-centred mechanism for diverse cellular components correlated with exhaustion.

Fri Apr 27 00:00:00 EDT 2018

Low-dose interleukin-2 promotes STAT-5 phosphorylation, T_reg survival and CTLA-4-dependent function in autoimmune liver diseases.

Clinical and experimental immunology

Jeffery HC,Jeffery LE,Lutz P,Corrigan M,Webb GJ,Hirschfield GM,Adams DH,Oo YH

12-3109 was used in Flow cytometry/Cell sorting to establish the potential of interleukin-2 to enhance regulatory T cell therapy in autoimmune liver disease.

Thu Jun 01 00:00:00 EDT 2017

NLRC3 is an inhibitory sensor of PI3K-mTOR pathways in cancer.

Nature

Karki R,Man SM,Malireddi RKS,Kesavardhana S,Zhu Q,Burton AR,Sharma BR,Qi X,Pelletier S,Vogel P,Rosenstiel P,Kanneganti TD

12-3109 was used in Flow cytometry/Cell sorting to explore a key role for NLRC3 in mediating protection against colorectal cancer.

Thu Dec 22 00:00:00 EST 2016

Alkylator-Induced and Patient-Derived Xenograft Mouse Models of Therapy-Related Myeloid Neoplasms Model Clinical Disease and Suggest the Presence of Multiple Cell Subpopulations with Leukemia Stem Cell Activity.

PloS one

Jonas BA,Johnson C,Gratzinger D,Majeti R

12-3109 was used in Flow cytometry/Cell sorting to report several new t-AML/MDS mouse models that could potentially be used to further define disease pathogenesis and test novel therapeutics.

Wed Jul 26 00:00:00 EDT 2017

Mice lacking Axl and Mer tyrosine kinase receptors are susceptible to experimental autoimmune orchitis induction.

Immunology and cell biology

Li N,Liu Z,Zhang Y,Chen Q,Liu P,Cheng CY,Lee WM,Chen Y,Han D

12-3109 was used in Flow cytometry to indicate that Axl and Mer receptors cooperatively regulate the systemic immune tolerance to male germ cell antigens.

Sun Mar 01 00:00:00 EST 2015

Identifying panaxynol, a natural activator of nuclear factor erythroid-2 related factor 2 (Nrf2) from American ginseng as a suppressor of inflamed macrophage-induced cardiomyocyte hypertrophy.

Journal of ethnopharmacology

Qu C,Li B,Lai Y,Li H,Windust A,Hofseth LJ,Nagarkatti M,Nagarkatti P,Wang XL,Tang D,Janicki JS,Tian X,Cui T

12-3109 was used in Flow cytometry/Cell sorting to demonstrate that panaxynol-activated Nrf2 signalling is at least partly responsible for American ginseng-induced health benefit in the heart.

Sat Jun 20 00:00:00 EDT 2015

Anti-melanoma vaccines engineered to simultaneously modulate cytokine priming and silence PD-L1 characterized using ex vivo myeloid-derived suppressor cells as a readout of therapeutic efficacy.

Oncoimmunology

Liechtenstein T,Perez-Janices N,Blanco-Luquin I,Goyvaerts C,Schwarze J,Dufait I,Lanna A,Ridder M,Guerrero-Setas D,Breckpot K,Escors D

12-3109 was used in Flow cytometry/Cell sorting to show that the simultaneous delivery of IL12 and a PD-L1-silencing shRNA exhibits therapeutically relevant anti-tumour activities.

Thu Feb 18 00:00:00 EST 2021

Bioinformatics

Protein Aliases: CD366; FLJ14428; HAVcr-2; Hepatitis A virus cellular receptor 2; kidney injury molecule-3; sCD366; soluble CD366; soluble TIM 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM-3; TIMD-3

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Gene Aliases: CD366; HAVcr-2; HAVCR2; KIM-3; Tim-3; TIM3; TIMD-3; TIMD3

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UniProt ID: (Human) Q8TDQ0

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Entrez Gene ID: (Human) 84868

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Function(s)

protein binding metal ion binding immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD366 (TIM3) Monoclonal Antibody (F38-2E2), PE, eBioscience™

CD366 (TIM3) Antibody (12-3109-42) in Flow

Product Details

12-3109-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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