Invitrogen

CD38 Monoclonal Antibody (90), eBioscience™

FIGURE: 1 / 22

CD38 Antibody (14-0381-82) in Flow

Staining of BALB/c splenocytes with 0.06 µg of Rat IgG2a Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.06 µg Anti-Mouse CD38 Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Total viable cells were used for analysis.

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Product Details

14-0381-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 18 publications 18 publications

Functional Assay (Functional)

Assay-Dependent

Mass Spectrometry (MS)

- -

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467219

Product Specific Information

Description: The 90 monoclonal antibody reacts with the mouse CD38 molecule, an ~42 kDa type II transmembrane protein. CD38 is expressed at increasingly higher levels on B cells at each stage of B-cell differentiation, and is then down-regulated on germinal center B cells and mature plasma cells. Its expression is reported on a subpopulation of thymocytes, mature T cells, and NK cells. Crosslinking of CD38 on the surface of mature, resting B cells induces B-cell proliferation, which is enhanced by co-signals such as IL-4 and LPS. CD38, a counter-receptor for CD31, is an ectoenzyme with cyclase and hydrolase enzymatic activity and is speculated to play a role in lymphocyte activation and differentiation.

Applications Reported: The 90 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. It has also been reported in functional assays. (Please use Functional Grade purified 90, Product # 16-0381, in functional assays).

Applications Tested: The 90 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Establishment and Characterization of Free-Floating 3D Macrophage Programming Model in the Presence of Cancer Cell Spheroids.

International journal of molecular sciences

Korotkaja K,Jansons J,Spunde K,Rudevica Z,Zajakina A

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Wed Jun 28 00:00:00 EDT 2023

Integrative Analysis Identified CD38 As a Key Node That Correlates Highly with Immunophenotype, Chemoradiotherapy Resistance, And Prognosis of Head and Neck Cancer.

Journal of Cancer

He Z,Yue C,Chen X,Li X,Zhang L,Tan S,Yi X,Luo G,Zhou Y

14-0381-82 was used in Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry (Paraffin) to identify and verify that CD38, which is closely related to the prognosis of HNC, is a potential biological marker of radiotherapy and chemotherapy resistance and PD-L1 immunotherapy resistance via a comprehensive bioinformatic analysis in The Cancer Genome Atlas and Gene Expression Omnibus databases.

Wed Jan 11 00:00:00 EST 2023

Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative regulator of B cell responses.

Proceedings of the National Academy of Sciences of the United States of America

Wang Y,Feswick A,Apostolou V,Petkov PM,Moser EK,Tibbetts SA

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2022

Proteoglycan 4 (PRG4) treatment enhances wound closure and tissue regeneration.

NPJ Regenerative medicine

Krawetz RJ,Abubacker S,Leonard C,Masson AO,Shah S,Narendran N,Tailor P,Regmi SC,Labit E,Ninkovic N,Corpuz JM,Ito K,Underhill TM,Salo PT,Schmidt TA,Biernaskie JA

14-0381-82 was used in Flow cytometry/Cell sorting to propose that rhPRG4 may represent a potential therapy to mitigate scar and improve wound healing.

Fri Jun 24 00:00:00 EDT 2022

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2021

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Pro-inflammatory immunity supports fibrosis advancement in epidermolysis bullosa: intervention with Ang-(1-7).

EMBO molecular medicine

Bernasconi R,Thriene K,Romero-Fernández E,Gretzmeier C,Kühl T,Maler M,Nauroy P,Kleiser S,Rühl-Muth AC,Stumpe M,Kiritsi D,Martin SF,Hinz B,Bruckner-Tuderman L,Dengjel J,Nyström A

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Thu Oct 07 00:00:00 EDT 2021

CD11b Deficiency Exacerbates Methicillin-Resistant Staphylococcus aureus-Induced Sepsis by Upregulating Inflammatory Responses of Macrophages.

Immune network

Sim H,Jeong D,Kim HI,Pak S,Thapa B,Kwon HJ,Lee K

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma.

Cancers

Nasarre P,Garcia DI,Siegel JB,Bonilla IV,Mukherjee R,Hilliard E,Chakraborty P,Nasarre C,Yustein JT,Lang M,Jaffa AA,Mehrotra S,Klauber-DeMore N

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Sun May 30 00:00:00 EDT 2021

Mycobacterial Trehalose 6,6'-Dimycolate-Induced M1-Type Inflammation.

The American journal of pathology

Nguyen TKT,d'Aigle J,Chinea L,Niaz Z,Hunter RL,Hwang SA,Actor JK

14-0381 was used in Immunohistochemistry to investigate the role of trehalose 6,6'-dimycolate (TDM) in the early macrophages response during initiation of granulomas of primary pathology.

Sat Feb 01 00:00:00 EST 2020

CHK1 dosage in germinal center B cells controls humoral immunity.

Cell death and differentiation

Schoeler K,Jakic B,Heppke J,Soratroi C,Aufschnaiter A,Hermann-Kleiter N,Villunger A,Labi V

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

DC3 is a method for deconvolution and coupled clustering from bulk and single-cell genomics data.

Nature communications

Zeng W,Chen X,Duren Z,Wang Y,Jiang R,Wong WH

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Thu Oct 10 00:00:00 EDT 2019

EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop.

Nature communications

Béguelin W,Rivas MA,Calvo Fernández MT,Teater M,Purwada A,Redmond D,Shen H,Challman MF,Elemento O,Singh A,Melnick AM

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2017

Robust growth of avirulent phase II Coxiella burnetii in bone marrow-derived murine macrophages.

PloS one

Cockrell DC,Long CM,Robertson SJ,Shannon JG,Miller HE,Myers L,Larson CL,Starr T,Beare PA,Heinzen RA

14038182 was used in flow cytometry to develop and describe reagents to study Coxiella-macrophage interactions

Wed Sep 06 00:00:00 EDT 2017

mTOR has distinct functions in generating versus sustaining humoral immunity.

The Journal of clinical investigation

Jones DD,Gaudette BT,Wilmore JR,Chernova I,Bortnick A,Weiss BM,Allman D

14038182 was used in flow cytometry to investigate the role of mTOR in plasma cell differentiation and antibody production in RAPTOR deficient cells.

Tue Nov 01 00:00:00 EDT 2016

Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization.

Nature communications

Bemark M,Hazanov H,Strömberg A,Komban R,Holmqvist J,Köster S,Mattsson J,Sikora P,Mehr R,Lycke NY

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Tue Sep 06 00:00:00 EDT 2016

BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.

PloS one

Funakoshi S,Shimizu T,Numata O,Ato M,Melchers F,Ohnishi K

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Wed Sep 16 00:00:00 EDT 2015

mTOR kinase inhibitors promote antibody class switching via mTORC2 inhibition.

Proceedings of the National Academy of Sciences of the United States of America

Limon JJ,So L,Jellbauer S,Chiu H,Corado J,Sykes SM,Raffatellu M,Fruman DA

14-0381 was used in Flow cytometry/Cell sorting to further elucidate the complexity of mTOR signalling in B cells.

Tue Nov 25 00:00:00 EST 2014

A disease-associated PTPN22 variant promotes systemic autoimmunity in murine models.

The Journal of clinical investigation

Dai X,James RG,Habib T,Singh S,Jackson S,Khim S,Moon RT,Liggitt D,Wolf-Yadlin A,Buckner JH,Rawlings DJ

Published figure using CD38 monoclonal antibody (Product # 14-0381-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen p45; Cyclic ADP-ribose hydrolase 1; I-19; NAD+ nucleosidase; NIM-R5 antigen

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Gene Aliases: ADPRC 1; Cd38; Cd38-rs1

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UniProt ID: (Mouse) P56528

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Entrez Gene ID: (Mouse) 12494

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Function(s)

NAD+ nucleosidase activity transferase activity hydrolase activity hydrolase activity, acting on glycosyl bonds phosphorus-oxygen lyase activity NAD(P)+ nucleosidase activity cyclase lyase metabolite interconversion enzyme

Process(es)

response to hypoxia positive regulation of cytosolic calcium ion concentration metabolic process response to hormone positive regulation of cell growth positive regulation of B cell proliferation positive regulation of insulin secretion response to hydroperoxide response to drug negative regulation of apoptotic process negative regulation of bone resorption negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of vasoconstriction B cell receptor signaling pathway long term synaptic depression

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD38 Monoclonal Antibody (90), eBioscience™

CD38 Antibody (14-0381-82) in Flow

Product Details

14-0381-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Functional Assay (Functional)

Mass Spectrometry (MS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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