Invitrogen

CD38 Monoclonal Antibody (90), Functional Grade, eBioscience™

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CD38 Antibody (16-0381-85) in Flow

International journal of molecular sciences 2023 - BMDMs reprogramming in the presence of 4T1/GFP cancer cell spheroid. ( a ) Experimental design for the reprogramming of free-floating M1 and M2 macrophages in the presence of cancer cell spheroid. BMDMs were activated for 48 h in 3D free-floating conditions to achieve M1 and M2 phenotypes. In parallel, 4T1/GFP murine mammary cancer cells were plated in a 96-well black round bottom ultra-low attachment plate... View More

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Product Details

16-0381-85

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 23 publications 23 publications

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468939

Product Specific Information

Description: The 90 monoclonal antibody reacts with the mouse CD38 molecule, an ~42 kDa type II transmembrane protein. CD38 is expressed at increasingly higher levels on B cells at each stage of B-cell differentiation, and is then down-regulated on germinal center B cells and mature plasma cells. Its expression is reported on a subpopulation of thymocytes, mature T cells, and NK cells. Crosslinking of CD38 on the surface of mature, resting B cells induces B-cell proliferation, which is enhanced by co-signals such as IL-4 and LPS. CD38, a counter-receptor for CD31, is an ectoenzyme with cyclase and hydrolase enzymatic activity and is speculated to play a role in lymphocyte activation and differentiation.

Applications Reported: The 90 antibody has been reported for use in flow cytometric analysis and functional assays.

Applications Tested: The 90 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Establishment and Characterization of Free-Floating 3D Macrophage Programming Model in the Presence of Cancer Cell Spheroids.

International journal of molecular sciences

Korotkaja K,Jansons J,Spunde K,Rudevica Z,Zajakina A

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Wed Jun 28 00:00:00 EDT 2023

Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative regulator of B cell responses.

Proceedings of the National Academy of Sciences of the United States of America

Wang Y,Feswick A,Apostolou V,Petkov PM,Moser EK,Tibbetts SA

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2022

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2021

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Pro-inflammatory immunity supports fibrosis advancement in epidermolysis bullosa: intervention with Ang-(1-7).

EMBO molecular medicine

Bernasconi R,Thriene K,Romero-Fernández E,Gretzmeier C,Kühl T,Maler M,Nauroy P,Kleiser S,Rühl-Muth AC,Stumpe M,Kiritsi D,Martin SF,Hinz B,Bruckner-Tuderman L,Dengjel J,Nyström A

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Thu Oct 07 00:00:00 EDT 2021

CD11b Deficiency Exacerbates Methicillin-Resistant Staphylococcus aureus-Induced Sepsis by Upregulating Inflammatory Responses of Macrophages.

Immune network

Sim H,Jeong D,Kim HI,Pak S,Thapa B,Kwon HJ,Lee K

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma.

Cancers

Nasarre P,Garcia DI,Siegel JB,Bonilla IV,Mukherjee R,Hilliard E,Chakraborty P,Nasarre C,Yustein JT,Lang M,Jaffa AA,Mehrotra S,Klauber-DeMore N

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Sun May 30 00:00:00 EDT 2021

B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches.

Cell reports

Biram A,Winter E,Denton AE,Zaretsky I,Dassa B,Bemark M,Linterman MA,Yaari G,Shulman Z

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Tue Feb 11 00:00:00 EST 2020

Antigen-presenting ILC3 regulate T cell-dependent IgA responses to colonic mucosal bacteria.

The Journal of experimental medicine

Melo-Gonzalez F,Kammoun H,Evren E,Dutton EE,Papadopoulou M,Bradford BM,Tanes C,Fardus-Reid F,Swann JR,Bittinger K,Mabbott NA,Vallance BA,Willinger T,Withers DR,Hepworth MR

16-0381-85 was used in Flow Cytometry to study the mechanisms that modulate the magnitude and quality of T cell-dependent IgA responses.

Mon Apr 01 00:00:00 EDT 2019

CHK1 dosage in germinal center B cells controls humoral immunity.

Cell death and differentiation

Schoeler K,Jakic B,Heppke J,Soratroi C,Aufschnaiter A,Hermann-Kleiter N,Villunger A,Labi V

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

DC3 is a method for deconvolution and coupled clustering from bulk and single-cell genomics data.

Nature communications

Zeng W,Chen X,Duren Z,Wang Y,Jiang R,Wong WH

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Thu Oct 10 00:00:00 EDT 2019

EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop.

Nature communications

Béguelin W,Rivas MA,Calvo Fernández MT,Teater M,Purwada A,Redmond D,Shen H,Challman MF,Elemento O,Singh A,Melnick AM

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2017

Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization.

Nature communications

Bemark M,Hazanov H,Strömberg A,Komban R,Holmqvist J,Köster S,Mattsson J,Sikora P,Mehr R,Lycke NY

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Tue Sep 06 00:00:00 EDT 2016

CD19 and BAFF-R can signal to promote B-cell survival in the absence of Syk.

The EMBO journal

Hobeika E,Levit-Zerdoun E,Anastasopoulou V,Pohlmeyer R,Altmeier S,Alsadeq A,Dobenecker MW,Pelanda R,Reth M

16-0381 was used in Flow cytometry/Cell sorting to investigate pathways responsible for the maintenance and survival of mature Syk-deficient B cells.

Wed Apr 01 00:00:00 EDT 2015

The E-Id protein axis modulates the activities of the PI3K-AKT-mTORC1-Hif1a and c-myc/p19Arf pathways to suppress innate variant TFH cell development, thymocyte expansion, and lymphomagenesis.

Genes & development

Miyazaki M,Miyazaki K,Chen S,Chandra V,Wagatsuma K,Agata Y,Rodewald HR,Saito R,Chang AN,Varki N,Kawamoto H,Murre C

16-0381 was used in Flow cytometry/Cell sorting to show that Id2 and Id3 suppress the development and expansion of innate variant follicular helper T cells.

Sun Feb 15 00:00:00 EST 2015

BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.

PloS one

Funakoshi S,Shimizu T,Numata O,Ato M,Melchers F,Ohnishi K

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Wed Sep 16 00:00:00 EDT 2015

Interleukin-35 administration counteracts established murine type 1 diabetes--possible involvement of regulatory T cells.

Scientific reports

Singh K,Kadesjö E,Lindroos J,Hjort M,Lundberg M,Espes D,Carlsson PO,Sandler S,Thorvaldson L

16-0381 was used in Flow cytometry/Cell sorting to suggest that insufficient IL-35 levels play a pivotal role in the development of T1D and that treatment with IL-35 should be investigated in treatment of T1D and other autoimmune diseases.

Thu Jul 30 00:00:00 EDT 2015

The role of the Syk/Shp-1 kinase-phosphatase equilibrium in B cell development and signaling.

Journal of immunology (Baltimore, Md. : 1950)

Alsadeq A,Hobeika E,Medgyesi D,Kläsener K,Reth M

16-0381 was used in Functional assays to display the importance of balance between kinases and phosphatases for immune cell function.

Tue Jul 01 00:00:00 EDT 2014

In vitro induced regulatory T cells are unique from endogenous regulatory T cells and effective at suppressing late stages of ongoing autoimmunity.

PloS one

Nguyen TL,Makhlouf NT,Anthony BA,Teague RM,DiPaolo RJ

16-0381 was used in Flow cytometry/Cell sorting to evaluate the ability of TGF-β-induced Tregs specific for the major self-antigen in autoimmune gastritis to suppress the established disease.

Mon May 11 00:00:00 EDT 2015

A disease-associated PTPN22 variant promotes systemic autoimmunity in murine models.

The Journal of clinical investigation

Dai X,James RG,Habib T,Singh S,Jackson S,Khim S,Moon RT,Liggitt D,Wolf-Yadlin A,Buckner JH,Rawlings DJ

Published figure using CD38 monoclonal antibody (Product # 16-0381-85) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

Critical role of SAP in progression and reactivation but not maintenance of T cell-dependent humoral immunity.

Molecular and cellular biology

Zhong MC,Veillette A

16-0381 was used in Flow cytometry/Cell sorting to explore the therapeutic potential of blocking the SLAM-associated protein (SAP) for human autoimmune diseases.

Fri Mar 01 00:00:00 EST 2013

Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas.

Journal of immunology (Baltimore, Md. : 1950)

Bolduc A,Long E,Stapler D,Cascalho M,Tsubata T,Koni PA,Shimoda M

16-0381 was used in Flow cytometry/Cell sorting to elucidate the role of CD40 signalling through cognate T-B interaction in the generation of germinal centre and memory B cells.

Thu Jul 01 00:00:00 EDT 2010

Tracking germinal center B cells expressing germ-line immunoglobulin gamma1 transcripts by conditional gene targeting.

Proceedings of the National Academy of Sciences of the United States of America

Casola S,Cattoretti G,Uyttersprot N,Koralov SB,Seagal J,Hao Z,Waisman A,Egert A,Ghitza D,Rajewsky K

16-0381 was used in Flow cytometry/Cell sorting to study gene function specifically in germinal centre B cells using Cγ1-cre knockin mice.

Tue May 09 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen p45; Cyclic ADP-ribose hydrolase 1; I-19; NAD+ nucleosidase; NIM-R5 antigen

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Gene Aliases: ADPRC 1; Cd38; Cd38-rs1

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UniProt ID: (Mouse) P56528

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Entrez Gene ID: (Mouse) 12494

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Function(s)

NAD+ nucleosidase activity transferase activity hydrolase activity hydrolase activity, acting on glycosyl bonds phosphorus-oxygen lyase activity NAD(P)+ nucleosidase activity cyclase lyase metabolite interconversion enzyme

Process(es)

response to hypoxia positive regulation of cytosolic calcium ion concentration metabolic process response to hormone positive regulation of cell growth positive regulation of B cell proliferation positive regulation of insulin secretion response to hydroperoxide response to drug negative regulation of apoptotic process negative regulation of bone resorption negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of vasoconstriction B cell receptor signaling pathway long term synaptic depression

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD38 Monoclonal Antibody (90), Functional Grade, eBioscience™

CD38 Antibody (16-0381-85) in Flow

Product Details

16-0381-85

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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