Invitrogen

CD38 Monoclonal Antibody (90), APC, eBioscience™

FIGURE: 1 / 20

CD38 Antibody (17-0381-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.03 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.03 µg of Anti-Mouse CD38 APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-0381-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469382

Product Specific Information

Description: The 90 monoclonal antibody reacts with the mouse CD38 molecule, an ~42 kDa type II transmembrane protein. CD38 is expressed at increasingly higher levels on B cells at each stage of B-cell differentiation, and is then down-regulated on germinal center B cells and mature plasma cells. Its expression is reported on a subpopulation of thymocytes, mature T cells, and NK cells. Crosslinking of CD38 on the surface of mature, resting B cells induces B-cell proliferation, which is enhanced by co-signals such as IL-4 and LPS. CD38, a counter-receptor for CD31, is an ectoenzyme with cyclase and hydrolase enzymatic activity and is speculated to play a role in lymphocyte activation and differentiation.

Applications Reported: The 90 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 90 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Establishment and Characterization of Free-Floating 3D Macrophage Programming Model in the Presence of Cancer Cell Spheroids.

International journal of molecular sciences

Korotkaja K,Jansons J,Spunde K,Rudevica Z,Zajakina A

Published figure using CD38 monoclonal antibody (Product # 17-0381-82) in Flow Cytometry

Wed Jun 28 00:00:00 EDT 2023

Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative regulator of B cell responses.

Proceedings of the National Academy of Sciences of the United States of America

Wang Y,Feswick A,Apostolou V,Petkov PM,Moser EK,Tibbetts SA

Published figure using CD38 monoclonal antibody (Product # 17-0381-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2022

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD38 monoclonal antibody (Product # 17-0381-82) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2021

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD38 monoclonal antibody (Product # 17-0381-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

A unified atlas of CD8 T cell dysfunctional states in cancer and infection.

Molecular cell

Pritykin Y,van der Veeken J,Pine AR,Zhong Y,Sahin M,Mazutis L,Pe'er D,Rudensky AY,Leslie CS

17-0381-82 was used in Flow cytometry/Cell sorting to show that our atlas of CD8 T cell states will facilitate mechanistic studies of T cell immunity and translational efforts.

Thu Jun 03 00:00:00 EDT 2021

Pro-inflammatory immunity supports fibrosis advancement in epidermolysis bullosa: intervention with Ang-(1-7).

EMBO molecular medicine

Bernasconi R,Thriene K,Romero-Fernández E,Gretzmeier C,Kühl T,Maler M,Nauroy P,Kleiser S,Rühl-Muth AC,Stumpe M,Kiritsi D,Martin SF,Hinz B,Bruckner-Tuderman L,Dengjel J,Nyström A

Published figure using CD38 monoclonal antibody (Product # 17-0381-82) in Flow Cytometry

Thu Oct 07 00:00:00 EDT 2021

CD11b Deficiency Exacerbates Methicillin-Resistant Staphylococcus aureus-Induced Sepsis by Upregulating Inflammatory Responses of Macrophages.

Immune network

Sim H,Jeong D,Kim HI,Pak S,Thapa B,Kwon HJ,Lee K

Published figure using CD38 monoclonal antibody (Product # 17-0381-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using CD38 monoclonal antibody (Product # 17-0381-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma.

Cancers

Nasarre P,Garcia DI,Siegel JB,Bonilla IV,Mukherjee R,Hilliard E,Chakraborty P,Nasarre C,Yustein JT,Lang M,Jaffa AA,Mehrotra S,Klauber-DeMore N

Published figure using CD38 monoclonal antibody (Product # 17-0381-82) in Flow Cytometry

Sun May 30 00:00:00 EDT 2021

TBL1XR1 Mutations Drive Extranodal Lymphoma by Inducing a Pro-tumorigenic Memory Fate.

Cell

Venturutti L,Teater M,Zhai A,Chadburn A,Babiker L,Kim D,Béguelin W,Lee TC,Kim Y,Chin CR,Yewdell WT,Raught B,Phillip JM,Jiang Y,Staudt LM,Green MR,Chaudhuri J,Elemento O,Farinha P,Weng AP,Nissen MD,Steidl C,Morin RD,Scott DW,Privé GG,Melnick AM

17-0381-82 was used in Flow Cytometry to show that TBL1XR1 mutations skew the humoral immune response toward generating abnormal immature memory B cells (MB), while impairing plasma cell differentiation.

Thu Jul 23 00:00:00 EDT 2020

Heterogenous Populations of Tissue-Resident CD8⁺ T Cells Are Generated in Response to Infection and Malignancy.

Immunity

Milner JJ,Toma C,He Z,Kurd NS,Nguyen QP,McDonald B,Quezada L,Widjaja CE,Witherden DA,Crowl JT,Shaw LA,Yeo GW,Chang JT,Omilusik KD,Goldrath AW

17-0381 was used in Flow cytometry/Cell sorting to identify discrete lineages of intestinal antigen-specific CD8+ T cells, including a Blimp1hiId3lo tissue-resident effector cell population most prominent in the early phase of acute viral and bacterial infections and a molecularly distinct Blimp1loId3hi tissue-resident memory population that subsequently accumulated at later infection time points.

Tue May 19 00:00:00 EDT 2020

Mutant EZH2 Induces a Pre-malignant Lymphoma Niche by Reprogramming the Immune Response.

Cancer cell

Béguelin W,Teater M,Meydan C,Hoehn KB,Phillip JM,Soshnev AA,Venturutti L,Rivas MA,Calvo-Fernández MT,Gutierrez J,Camarillo JM,Takata K,Tarte K,Kelleher NL,Steidl C,Mason CE,Elemento O,Allis CD,Kleinstein SH,Melnick AM

17-0381 was used in Flow cytometry/Cell sorting to show that EZH2 mutations initiate FL by attenuating germinal center(GC) B cell requirement for T cell help and driving slow expansion of GC centrocytes that become enmeshed with and dependent on follicular dendritic cells.

Mon May 11 00:00:00 EDT 2020

Non-oncogene Addiction to SIRT3 Plays a Critical Role in Lymphomagenesis.

Cancer cell

Li M,Chiang YL,Lyssiotis CA,Teater MR,Hong JY,Shen H,Wang L,Hu J,Jing H,Chen Z,Jain N,Duy C,Mistry SJ,Cerchietti L,Cross JR,Cantley LC,Green MR,Lin H,Melnick AM

17-0381 was used in Flow cytometry/Cell sorting to investigate if diffuse large B cell lymphomas are dependent on mitochondrial lysine deacetylase SIRT3 for proliferation, survival, self-renewal, and tumor growth in vivo regardless of disease subtype and genetics.

Mon Jun 10 00:00:00 EDT 2019

Histone demethylase LSD1 is required for germinal center formation and BCL6-driven lymphomagenesis.

Nature immunology

Hatzi K,Geng H,Doane AS,Meydan C,LaRiviere R,Cardenas M,Duy C,Shen H,Vidal MNC,Baslan T,Mohammad HP,Kruger RG,Shaknovich R,Haberman AM,Inghirami G,Lowe SW,Melnick AM

17-0381 was used in Flow cytometry/Cell sorting to demonstrate that LSD1 modulates enhancer function by forming repression complexes with BCL6 during the humoral immune response.

Tue Jan 01 00:00:00 EST 2019

CHK1 dosage in germinal center B cells controls humoral immunity.

Cell death and differentiation

Schoeler K,Jakic B,Heppke J,Soratroi C,Aufschnaiter A,Hermann-Kleiter N,Villunger A,Labi V

Published figure using CD38 monoclonal antibody (Product # 17-0381-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop.

Nature communications

Béguelin W,Rivas MA,Calvo Fernández MT,Teater M,Purwada A,Redmond D,Shen H,Challman MF,Elemento O,Singh A,Melnick AM

17-0381 was used in Flow cytometry/Cell sorting to show EZH2 mediates germinal centre formation through epigenetic silencing of CDKN1A and release of cell cycle checkpoints.

Thu Oct 12 00:00:00 EDT 2017

Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization.

Nature communications

Bemark M,Hazanov H,Strömberg A,Komban R,Holmqvist J,Köster S,Mattsson J,Sikora P,Mehr R,Lycke NY

Published figure using CD38 monoclonal antibody (Product # 17-0381-82) in Flow Cytometry

Tue Sep 06 00:00:00 EDT 2016

Angiotensin converting enzyme inhibitors and angiotensin II receptor antagonist attenuate tumor growth via polarization of neutrophils toward an antitumor phenotype.

Oncoimmunology

Shrestha S,Noh JM,Kim SY,Ham HY,Kim YJ,Yun YJ,Kim MJ,Kwon MS,Song DK,Hong CW

17-0381 was used in Flow cytometry/Cell sorting to investigate how modulation of Ang II pathway attenuates tumour growth via polarisation of neutrophils to an antitumoural phenotype.

Tue Jan 12 00:00:00 EST 2021

BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.

PloS one

Funakoshi S,Shimizu T,Numata O,Ato M,Melchers F,Ohnishi K

Published figure using CD38 monoclonal antibody (Product # 17-0381-82) in Flow Cytometry

Wed Sep 16 00:00:00 EDT 2015

Mir-17-92 regulates bone marrow homing of plasma cells and production of immunoglobulin G2c.

Nature communications

Xu S,Ou X,Huo J,Lim K,Huang Y,Chee S,Lam KP

17-0381 was used in Flow cytometry/Cell sorting to identify important roles for mir-17-92 in the regulation of peripheral B-cell function.

Fri Apr 17 00:00:00 EDT 2015

Characterization of cardiac-resident progenitor cells expressing high aldehyde dehydrogenase activity.

BioMed research international

Roehrich ME,Spicher A,Milano G,Vassalli G

17-0381 was used in Flow cytometry/Cell sorting to investigate the presence of cardiac-resident progenitor cells with high aldehyde dehydrogenase activity isolated from mouse hearts.

Tue Sep 03 00:00:00 EDT 2013

Use of a virus-encoded enzymatic marker reveals that a stable fraction of memory B cells expresses latency-associated nuclear antigen throughout chronic gammaherpesvirus infection.

Journal of virology

Nealy MS,Coleman CB,Li H,Tibbetts SA

17-0381 was used in Flow cytometry/Cell sorting to investigate the expression of LANA/EBNA-1 in individual B-cell subsets through the course of chronic gammaherpesvirus infection, showing that a stable fraction of memory B cells expresses LANA.

Sun Aug 01 00:00:00 EDT 2010

Impaired germinal center responses and suppression of local IgG production during intracellular bacterial infection.

Journal of immunology (Baltimore, Md. : 1950)

Racine R,Jones DD,Chatterjee M,McLaughlin M,Macnamara KC,Winslow GM

17-0381 was used in Flow cytometry/Cell sorting to provide a mechanism whereby an intracellular bacterial infection can compromise local immunity to coinfecting pathogens or antigenic challenge.

Sat May 01 00:00:00 EDT 2010

T-bet concomitantly controls migration, survival, and effector functions during the development of Valpha14i NKT cells.

Blood

Matsuda JL,Zhang Q,Ndonye R,Richardson SK,Howell AR,Gapin L

17-0381 was used in Flow cytometry/Cell sorting to identify some of the genetic targets of T-bet during V14i NKT-cell lineage development.

Sat Apr 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen p45; Cyclic ADP-ribose hydrolase 1; I-19; NAD+ nucleosidase; NIM-R5 antigen

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Gene Aliases: ADPRC 1; Cd38; Cd38-rs1

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UniProt ID: (Mouse) P56528

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Entrez Gene ID: (Mouse) 12494

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Function(s)

NAD+ nucleosidase activity transferase activity hydrolase activity hydrolase activity, acting on glycosyl bonds phosphorus-oxygen lyase activity NAD(P)+ nucleosidase activity cyclase lyase metabolite interconversion enzyme

Process(es)

response to hypoxia positive regulation of cytosolic calcium ion concentration metabolic process response to hormone positive regulation of cell growth positive regulation of B cell proliferation positive regulation of insulin secretion response to hydroperoxide response to drug negative regulation of apoptotic process negative regulation of bone resorption negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of vasoconstriction B cell receptor signaling pathway long term synaptic depression

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CD38 Monoclonal Antibody (90), APC, eBioscience™

CD38 Antibody (17-0381-82) in Flow

Product Details

17-0381-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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