Invitrogen

CD40 Monoclonal Antibody (5C3), PerCP-eFluor™ 710, eBioscience™

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CD40 Antibody (46-0409-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or Anti-Human CD40 PerCP-eFluor® 710 (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

46-0409-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.03 µg)/test

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

5C3

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834403

Product Specific Information

Description: The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.

Applications Reported: This 5C3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 5C3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

SIDT1 plays a key role in type I IFN responses to nucleic acids in plasmacytoid dendritic cells and mediates the pathogenesis of an imiquimod-induced psoriasis model.

EBioMedicine

Morell M,Varela N,Castillejo-López C,Coppard C,Luque MJ,Wu YY,Martín-Morales N,Pérez-Cózar F,Gómez-Hernández G,Kumar R,O'Valle F,Alarcón-Riquelme ME,Marañón C

46-0409-42 was used in Flow Cytometry to explore the role of SIDT1, an ER-resident protein expressed in the lymphoid lineage and involved in anti-viral IFN-I responses in vivo, in the main IFN-producing cells, the plasmacytoid dendritic cells.

Tue Feb 01 00:00:00 EST 2022

Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells.

Reproductive biology and endocrinology : RB&E

Li L,Liao Z,Ye M,Jiang J

Published figure using CD40 monoclonal antibody (Product # 46-0409-42) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Activated monocytes and markers of inflammation in newly diagnosed multiple sclerosis.

Immunology and cell biology

Carstensen M,Christensen T,Stilund M,Møller HJ,Petersen EL,Petersen T

Published figure using CD40 monoclonal antibody (Product # 46-0409-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Subsets of activated monocytes and markers of inflammation in incipient and progressed multiple sclerosis.

Immunology and cell biology

Gjelstrup MC,Stilund M,Petersen T,Møller HJ,Petersen EL,Christensen T

46-0409 was used in Flow cytometry/Cell sorting to investigate the involvement of peripheral blood mononuclear cells in multiple sclerosis.

Thu Feb 01 00:00:00 EST 2018

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

Published figure using CD40 monoclonal antibody (Product # 46-0409-42) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis.

Frontiers in immunology

Lopes DM,Oliveira SC,Page B,Carvalho LP,Carvalho EM,Cardoso LS

46-0409 was used in Flow cytometry/Cell sorting to evaluate the potential of the S.mansoni Sm29 antigen to change aspects of profiles of monocyte derived dendritic cells and lymphocytes from subjects with cutaneous leishmaniasis.

Tue Oct 15 00:00:00 EDT 2019

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

Oncotarget

Pathangey LB,McCurry DB,Gendler SJ,Dominguez AL,Gorman JE,Pathangey G,Mihalik LA,Dang Y,Disis ML,Cohen PA

Published figure using CD40 monoclonal antibody (Product # 46-0409-42) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2017

A simple, accurate and universal method for quantification of PCR.

BMC biotechnology

Boulter N,Suarez FG,Schibeci S,Sunderland T,Tolhurst O,Hunter T,Hodge G,Handelsman D,Simanainen U,Hendriks E,Duggan K

Published figure using CD40 monoclonal antibody (Product # 46-0409-42) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2016

A novel subset of B7-H3⁺CD14⁺HLA-DR^-/low myeloid-derived suppressor cells are associated with progression of human NSCLC.

Oncoimmunology

Zhang G,Huang H,Zhu Y,Yu G,Gao X,Xu Y,Liu C,Hou J,Zhang X

Published figure using CD40 monoclonal antibody (Product # 46-0409-42) in Flow Cytometry

Sun Feb 01 00:00:00 EST 2015

NF-κB, p38 MAPK, ERK1/2, mTOR, STAT3 and increased glycolysis regulate stability of paricalcitol/dexamethasone-generated tolerogenic dendritic cells in the inflammatory environment.

Oncotarget

Dáňová K,Klapetková A,Kayserová J,Šedivá A,Špíšek R,Jelínková LP

46-0409 was used in Flow cytometry/Cell sorting to investigate the signalling pathways regulating the properties of tolerogenic dendritic cells in the inflammatory environment.

Wed Jun 10 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; Tumor necrosis factor receptor superfamily member 5

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Gene Aliases: Bp50; CD40; CDW40; p50; TNFRSF5

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UniProt ID: (Human) P25942

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Entrez Gene ID: (Human) 958

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Function(s)

antigen binding signal transducer activity receptor activity tumor necrosis factor-activated receptor activity protein binding enzyme binding ubiquitin protein ligase binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD40 Monoclonal Antibody (5C3), PerCP-eFluor™ 710, eBioscience™

CD40 Antibody (46-0409-42) in Flow

Product Details

46-0409-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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