Invitrogen

CD41a Monoclonal Antibody (HIP8), PE, eBioscience™

FIGURE: 1 / 3

CD41a Antibody (12-0419-42) in Flow

Staining of human platelets with Anti-Human CD42b FITC (Product # 11-0429-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD41a PE (right). Total viable cells were used for analysis.

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Product Details

12-0419-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human

Published species

Baboon, Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HIP8

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10870785

Product Specific Information

Description: The HIP8 monoclonal antibody reacts with the human CD41 molecule, the integrin alpha IIb also known as platelet GPIIb. CD41 non-covalently associates with integrin beta3 (GPIIIa, CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin and mediates platelets aggregation. HIP8 blocks platelet aggregation.

Applications Reported: The HIP8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIP8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD41 (platelet glycoprotein IIb, ITGA2B) is composed of two subunits -120 kDa a, alpha and 23 kDa b, beta- that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. CD41 is also involved in blood coagulation by mediating platelet aggregation. Upon blood vessel damage, CD41 binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during the early stages of hematopoietic differentiation. Diseases associated with CD41 dysfunction include Glansmann Thrombasthenia, and Platelet type-16 bleeding disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD41a monoclonal antibody (Product # 12-0419-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Common variants in signaling transcription-factor-binding sites drive phenotypic variability in red blood cell traits.

Nature genetics

Choudhuri A,Trompouki E,Abraham BJ,Colli LM,Kock KH,Mallard W,Yang ML,Vinjamur DS,Ghamari A,Sporrij A,Hoi K,Hummel B,Boatman S,Chan V,Tseng S,Nandakumar SK,Yang S,Lichtig A,Superdock M,Grimes SN,Bowman TV,Zhou Y,Takahashi S,Joehanes R,Cantor AB,Bauer DE,Ganesh SK,Rinn J,Albert PS,Bulyk ML,Chanock SJ,Young RA,Zon LI

12-0419 was used in Flow cytometry/Cell sorting to propose that the majority of the RBC-trait-associated variants that reside on transcription-factor-binding sequences fall in STF target sequences, suggesting that the phenotypic variation of RBC traits could stem from altered responsiveness to extracellular stimuli.

Tue Dec 01 00:00:00 EST 2020

Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis.

Cancers

Zimmer N,Krebs FK,Zimmer S,Mitzel-Rink H,Kumm EJ,Jurk K,Grabbe S,Loquai C,Tuettenberg A

Published figure using CD41a monoclonal antibody (Product # 12-0419-42) in Flow Cytometry

Sat Dec 05 00:00:00 EST 2020

Mitochondrial DNA in the tumour microenvironment activates neutrophils and is associated with worse outcomes in patients with advanced epithelial ovarian cancer.

British journal of cancer

Singel KL,Grzankowski KS,Khan ANMNH,Grimm MJ,D'Auria AC,Morrell K,Eng KH,Hylander B,Mayor PC,Emmons TR,Lénárt N,Fekete R,Környei Z,Muthukrishnan U,Gilthorpe JD,Urban CF,Itagaki K,Hauser CJ,Leifer C,Moysich KB,Odunsi K,Dénes Á,Segal BH

12-0419 was used in Flow cytometry/Cell sorting to hypothesize that extracellular mitochondrial DNA in ascites from patients with epithelial ovarian cancer would correlate with worse outcomes.

Tue Jan 01 00:00:00 EST 2019

Mesenchymal stromal cells lower platelet activation and assist in platelet formation in vitro.

JCI insight

Mendelson A,Strat AN,Bao W,Rosston P,Fallon G,Ohrn S,Zhong H,Lobo C,An X,Yazdanbakhsh K

12-0419-42 was used in Flow Cytometry to develop a potentially novel culture system for supporting platelet formation in vitro using a unique population of CD51+PDGFRα+ perivascular cells, derived from human umbilical cord tissue, which display numerous mesenchymal stem cell (MSC) properties.

Thu Aug 22 00:00:00 EDT 2019

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

12-0419-42 was used in Flow Cytometry to identify 77 genes at 38 GWAS loci, with most loci harboring 1-2 candidate genes.

Thu May 09 00:00:00 EDT 2019

Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation.

Cell

Kim AR,Ulirsch JC,Wilmes S,Unal E,Moraga I,Karakukcu M,Yuan D,Kazerounian S,Abdulhay NJ,King DS,Gupta N,Gabriel SB,Lander ES,Patiroglu T,Ozcan A,Ozdemir MA,Garcia KC,Piehler J,Gazda HT,Klein DE,Sankaran VG

12-0419 was used in Flow cytometry/Cell sorting to demonstrate how variation in a single cytokine can lead to biased downstream signaling and can thereby cause human disease.

Thu Mar 09 00:00:00 EST 2017

Antithymocyte Globulin at Clinically Relevant Concentrations Kills Leukemic Blasts.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Dabas R,Lee R,Servito MT,Dharmani-Khan P,Modi M,van Slyke T,Luider J,Durand C,Larratt L,Brandwein J,Morris D,Daly A,Khan FM,Storek J

Sun May 01 00:00:00 EDT 2016

Mesenchymal stromal cells express GARP/LRRC32 on their surface: effects on their biology and immunomodulatory capacity.

Stem cells (Dayton, Ohio)

Carrillo-Galvez AB,Cobo M,Cuevas-Ocaña S,Gutiérrez-Guerrero A,Sánchez-Gilabert A,Bongarzone P,García-Pérez A,Muñoz P,Benabdellah K,Toscano MG,Martín F,Anderson P

12-0419 was used in Flow cytometry/Cell sorting to show that human and mouse mesenchymal stromal cells express glycoprotein A repetitions predominant, which presents LAP/TGF-β1 on their cell surface.

Thu Jan 01 00:00:00 EST 2015

Altered translation of GATA1 in Diamond-Blackfan anemia.

Nature medicine

Ludwig LS,Gazda HT,Eng JC,Eichhorn SW,Thiru P,Ghazvinian R,George TI,Gotlib JR,Beggs AH,Sieff CA,Lodish HF,Lander ES,Sankaran VG

12-0419 was used in Flow cytometry/Cell sorting to study the altered translation of GATA1 in cells isolated from patients with Diamond-Blackfan anemia.

Tue Jul 01 00:00:00 EDT 2014

Prasugrel metabolites inhibit neutrophil functions.

The Journal of pharmacology and experimental therapeutics

Liverani E,Rico MC,Garcia AE,Kilpatrick LE,Kunapuli SP

12-0419 was used in Flow cytometry/Cell sorting to investigate the effect of prasugrel metabolites on neutrophils to further clarify the role of thienopyridines in inflammation.

Tue Jan 01 00:00:00 EST 2013

Potential factors influencing the development of thrombocytopenia and consumptive coagulopathy after genetically modified pig liver xenotransplantation.

Transplant international : official journal of the European Society for Organ Transplantation

Ekser B,Lin CC,Long C,Echeverri GJ,Hara H,Ezzelarab M,Bogdanov VY,Stolz DB,Enjyoji K,Robson SC,Ayares D,Dorling A,Cooper DK,Gridelli B

12-0419 was used in Flow cytometry/Cell sorting to reveal tissue factor exposure on recipient platelets and peripheral blood mononuclear cells as a potential key factor in the development of thrombocytopenia.

Wed Aug 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: alphaIIb protein; CD41; form 1; form 2; GPalpha IIb; GPIIb; Integrin alpha-IIb; integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41); platelet fibrinogen receptor, alpha subunit; platelet glycoprotein IIb of IIb/IIIa complex; Platelet membrane glycoprotein IIb; platelet-specific antigen BAK; protein phosphatase 1, regulatory subunit 93

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Gene Aliases: BDPLT16; BDPLT2; CD41; CD41B; GP2B; GPIIb; GT; GTA; HPA3; ITGA2B; ITGAB; PPP1R93

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UniProt ID: (Human) P08514

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Entrez Gene ID: (Human) 3674

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Function(s)

protein binding identical protein binding metal ion binding extracellular matrix binding fibrinogen binding integrin

Process(es)

platelet degranulation positive regulation of leukocyte migration cell adhesion cell-matrix adhesion integrin-mediated signaling pathway extracellular matrix organization platelet aggregation

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CD41a Monoclonal Antibody (HIP8), PE, eBioscience™

CD41a Antibody (12-0419-42) in Flow

Product Details

12-0419-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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