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CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), FITC, eBioscience™

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Antibody Testing Data (1)
Published Figures (14)

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CD43 Antibody (11-0439-42) in Flow

Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, eFluor 450 (Product # 48-0038-42) and Mouse IgG1 kappa Isotype Control, FITC (Product # 11-4714-82) (left) or CD43 Monoclonal Antibody, FITC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

11-0439-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Fish, Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio84-3C1 (84-3C1)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717075

Product Specific Information

Description: The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.

Applications Reported: This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio84-3C1 (84-3C1) antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood mononuclear cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability. CD43 appears to be an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation.

STAR protocols

Garcia-Alegria E,Potts B,Menegatti S,Kouskoff V

Published figure using CD43 monoclonal antibody (Product # 11-0439-42) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Human and Mouse Transcriptome Profiling Identifies Cross-Species Homology in Pulmonary and Lymph Node Mononuclear Phagocytes.

Cell reports

Leach SM,Gibbings SL,Tewari AD,Atif SM,Vestal B,Danhorn T,Janssen WJ,Wager TD,Jakubzick CV

11-0439 was used in Flow cytometry/Cell sorting to created a gene expression compendium across 24 distinct human and murine lung and lymph node mononuclear phagocyte (MP), along with human blood and murine spleen MPs, to serve as validation datasets.

Tue Nov 03 00:00:00 EST 2020

Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model.

Journal of human genetics

Ichisima J,Suzuki NM,Samata B,Awaya T,Takahashi J,Hagiwara M,Nakahata T,Saito MK

11-0439 was used in Flow cytometry/Cell sorting to conclude that the iPSC model revealed a novel effect of the ATR mutation in mitotic processes of NPCs and NPC-specific missplicing, accompanied by the recovery of neuronal defects using a splicing rectifier.

Wed May 01 00:00:00 EDT 2019

Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model.

Journal of human genetics

Ichisima J,Suzuki NM,Samata B,Awaya T,Takahashi J,Hagiwara M,Nakahata T,Saito MK

Wed May 01 00:00:00 EDT 2019

The Generation of Human γδT Cell-Derived Induced Pluripotent Stem Cells from Whole Peripheral Blood Mononuclear Cell Culture.

Stem cells translational medicine

Watanabe D,Koyanagi-Aoi M,Taniguchi-Ikeda M,Yoshida Y,Azuma T,Aoi T

Published figure using CD43 monoclonal antibody (Product # 11-0439-42) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 11-0439-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease.

Biomedical reports

Yoon JY,Lee Y,Yu SL,Yoon HK,Park HY,Joung CI,Park SR,Kwon M,Kang J

Published figure using CD43 monoclonal antibody (Product # 11-0439-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 11-0439-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD43 monoclonal antibody (Product # 11-0439-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

Published figure using CD43 monoclonal antibody (Product # 11-0439-42) in Flow Cytometry

Thu Jul 17 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD43; Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; Sialophorin; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115

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Gene Aliases: CD43; GALGP; GPL115; LSN; SPN

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UniProt ID: (Human) P16150

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Entrez Gene ID: (Human) 6693

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Function(s)

transmembrane signaling receptor activity

Process(es)

response to protozoan negative regulation of type IV hypersensitivity positive regulation of protein phosphorylation chemotaxis immune response cellular defense response negative regulation of cell adhesion establishment or maintenance of cell polarity signal transduction cell surface receptor signaling pathway T cell costimulation positive regulation of T cell proliferation negative regulation of T cell proliferation positive regulation of tumor necrosis factor biosynthetic process defense response to bacterium negative thymic T cell selection regulation of defense response to virus leukocyte migration apoptotic signaling pathway

Performance Guarantee

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CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), FITC, eBioscience™

CD43 Antibody (11-0439-42) in Flow

Product Details

11-0439-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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