Invitrogen

CD45 Monoclonal Antibody (30-F11), Pacific Orange™

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CD45 Antibody (MCD4530) in Flow

Scientific reports 2018 - Figure 5 Peripheral and central immune cell phenotypes are not altered following anti-TNFR1 treatment. MNCs, isolated from the CNS (pooled brain and spinal cord; ( A ), and splenocytes ( C ) were collected on days 5 and 6 of acute EAE from mice treated with either ATROSAB or control IgG. Following gating for CD45 + CD4 + cells, FACS analysis was performed to determine the percentage of those co-expressing... View More

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Product Details

MCD4530

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-Dependent

Flow Cytometry (Flow)

Assay-dependent

View 16 publications 16 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

30-F11

Immunogen

The CD45 antigen

Conjugate

Pacific Orange™ Pacific Orange™ Pacific Orange™

Excitation/Emission Max

400/551 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539700

Product Specific Information

Applications reported for the Rat anti-Mouse CD45 mAb include flow cytometry, immunohistochemistry (zinc- and formalin-fixed, paraffin-embedded), and immunohistochemistry (acetone-fixed, frozen). If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Cells should be analyzed within 18 hours of staining for best results. Avoid light exposure with fluorochrome-conjugated antibodies. Use dim light during handling, incubation with cells, and prior to analysis.

For Pacific Orange™ conjugates: excitation 405 nm/peak emission 551 nm. Matching isotype control is available: Rt IgG2b Pacific Orange™.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Macrophage-intrinsic DUOX1 contributes to type 2 inflammation and mucus metaplasia during allergic airway disease.

Mucosal immunology

Morris CR,Habibovic A,Dustin CM,Schiffers C,Lin MC,Ather JL,Janssen-Heininger YMW,Poynter ME,Utermohlen O,Krönke M,van der Vliet A

MCD4530 was used in Flow cytometry/Cell sorting to reveal a contribution of macrophage-intrinsic DUOX1 in macrophage recruitment upon chronic HDM challenge, as well as features of macrophage activation that impact on type 2 inflammation and remodeling.

Sun May 01 00:00:00 EDT 2022

Tumor Cell-Derived Angiopoietin-2 Promotes Metastasis in Melanoma.

Cancer research

Abdul Pari AA,Singhal M,Hübers C,Mogler C,Schieb B,Gampp A,Gengenbacher N,Reynolds LE,Terhardt D,Géraud C,Utikal J,Thomas M,Goerdt S,Hodivala-Dilke KM,Augustin HG,Felcht M

MCD4530 was used in Flow Cytometry to reveal that tumor cells can be a source of ANGPT2 in the tumor microenvironment and that tumor cell-derived ANGPT2 augments metastatic colonization by protecting tumor cells from oxidative stress.

Mon Jun 15 00:00:00 EDT 2020

Neutrophil infiltration regulates clock-gene expression to organize daily hepatic metabolism.

eLife

Crespo M,Gonzalez-Teran B,Nikolic I,Mora A,Folgueira C,Rodríguez E,Leiva-Vega L,Pintor-Chocano A,Fernández-Chacón M,Ruiz-Garrido I,Cicuéndez B,Tomás-Loba A,A-Gonzalez N,Caballero-Molano A,Beiroa D,Hernández-Cosido L,Torres JL,Kennedy NJ,Davis RJ,Benedito R,Marcos M,Nogueiras R,Hidalgo A,Matesanz N,Leiva M,Sabio G

MCD4530 was used in Flow cytometry/Cell sorting to demonstrate that neutrophils contribute to the maintenance of daily hepatic homeostasis through the regulation of the neutrophil elastase/c-Jun NH2-terminal kinase/Bmal1 (NE/JNK/Bmal1) axis.

Tue Dec 08 00:00:00 EST 2020

Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.

Cell metabolism

Halbrook CJ,Pontious C,Kovalenko I,Lapienyte L,Dreyer S,Lee HJ,Thurston G,Zhang Y,Lazarus J,Sajjakulnukit P,Hong HS,Kremer DM,Nelson BS,Kemp S,Zhang L,Chang D,Biankin A,Shi J,Frankel TL,Crawford HC,Morton JP,Pasca di Magliano M,Lyssiotis CA

MCD4530 was used in Flow cytometry/Cell sorting to characterise the role of macrophages in pancreatic ductal adenocarcinoma and possibilities for pancreatic cancer therapy.

Tue Jun 04 00:00:00 EDT 2019

Aerobic Glycolysis Controls Myeloid-Derived Suppressor Cells and Tumor Immunity via a Specific CEBPB Isoform in Triple-Negative Breast Cancer.

Cell metabolism

Li W,Tanikawa T,Kryczek I,Xia H,Li G,Wu K,Wei S,Zhao L,Vatan L,Wen B,Shu P,Sun D,Kleer C,Wicha M,Sabel M,Tao K,Wang G,Zou W

MCD4530 was used in Flow cytometry/Cell sorting to study the mechanisms by which aerobic glycolysis in triple-negative breast cancer tumours controls myeloid-derived suppressor cells and immunosuppression via a CEBPB pathway.

Tue Jul 03 00:00:00 EDT 2018

Anti-TNFR1 targeting in humanized mice ameliorates disease in a model of multiple sclerosis.

Scientific reports

Williams SK,Fairless R,Maier O,Liermann PC,Pichi K,Fischer R,Eisel ULM,Kontermann R,Herrmann A,Weksler B,Romero N,Couraud PO,Pfizenmaier K,Diem R

Published figure using CD45 monoclonal antibody (Product # MCD4530) in Flow Cytometry

Tue Sep 11 00:00:00 EDT 2018

Blocking type I interferon signaling enhances T cell recovery and reduces HIV-1 reservoirs.

The Journal of clinical investigation

Cheng L,Ma J,Li J,Li D,Li G,Li F,Zhang Q,Yu H,Yasui F,Ye C,Tsao LC,Hu Z,Su L,Zhang L

MCD4530 was used in flow cytometry to study the impact of modulating IFN-I signaling during suppressive combined antiretroviral therapy

Tue Jan 03 00:00:00 EST 2017

Glycosylphosphatidylinositol-Anchored Anti-HIV scFv Efficiently Protects CD4 T Cells from HIV-1 Infection and Deletion in hu-PBL Mice.

Journal of virology

Ye C,Wang W,Cheng L,Li G,Wen M,Wang Q,Zhang Q,Li D,Zhou P,Su L

MCD4530 was used in Flow cytometry/Cell sorting to conclude that GPI-scFv X5-modified CD4 T cells could potentially be used as a genetic intervention against both R5- and X4-tropic HIV-1 infections.

Wed Feb 01 00:00:00 EST 2017

Collectin-11 detects stress-induced L-fucose pattern to trigger renal epithelial injury.

The Journal of clinical investigation

Farrar CA,Tran D,Li K,Wu W,Peng Q,Schwaeble W,Zhou W,Sacks SH

MCD4530 was used in Immunohistochemistry-immunofluorescence to describe how collectin-11 recognises an abnormal pattern of L-fucose on postischemic renal tubule cells and activates a destructive inflammatory response.

Mon May 02 00:00:00 EDT 2016

In vivo nanoparticle imaging of innate immune cells can serve as a marker of disease severity in a model of multiple sclerosis.

Proceedings of the National Academy of Sciences of the United States of America

Kirschbaum K,Sonner JK,Zeller MW,Deumelandt K,Bode J,Sharma R,Krüwel T,Fischer M,Hoffmann A,Costa da Silva M,Muckenthaler MU,Wick W,Tews B,Chen JW,Heiland S,Bendszus M,Platten M,Breckwoldt MO

MCD4530 was used in flow cytometry to use MRI to track iron oxide nanoparticles in vivo

Tue Nov 15 00:00:00 EST 2016

General control non-derepressible 2 (GCN2) in T cells controls disease progression of autoimmune neuroinflammation.

Journal of neuroimmunology

Keil M,Sonner JK,Lanz TV,Oezen I,Bunse T,Bittner S,Meyer HV,Meuth SG,Wick W,Platten M

MCD4530 was used in flow cytometry to investigate the role of general control non-derepressible 2 in resolving autoimmune neuroinflammation

Mon Aug 15 00:00:00 EDT 2016

Eradication of metastatic mouse cancers resistant to immune checkpoint blockade by suppression of myeloid-derived cells.

Proceedings of the National Academy of Sciences of the United States of America

Kim K,Skora AD,Li Z,Liu Q,Tam AJ,Blosser RL,Diaz LA,Papadopoulos N,Kinzler KW,Vogelstein B,Zhou S

MCD4530 was used in flow cytometry to identify a relationship between resistant cancers and myeloid-derived suppressor cells

Tue Aug 12 00:00:00 EDT 2014

CD4+ T lymphocyte ablation prevents pancreatic carcinogenesis in mice.

Cancer immunology research

Zhang Y,Yan W,Mathew E,Bednar F,Wan S,Collins MA,Evans RA,Welling TH,Vonderheide RH,di Magliano MP

MCD4530 was used in flow cytometry to determine the role of CD4+ T cells during mutant Kras-driven pancreatic carcinogenesis

Thu May 01 00:00:00 EDT 2014

Myeloid cell-specific ATP-binding cassette transporter A1 deletion has minimal impact on atherogenesis in atherogenic diet-fed low-density lipoprotein receptor knockout mice.

Arteriosclerosis, thrombosis, and vascular biology

Bi X,Zhu X,Gao C,Shewale S,Cao Q,Liu M,Boudyguina E,Gebre AK,Wilson MD,Brown AL,Parks JS

MCD4530 was used in Flow cytometry/Cell sorting to assess the atherosclerotic impact of myeloid cell ATP-binding cassette transporter A1.

Mon Sep 01 00:00:00 EDT 2014

Impact of isolated germline JAK2V617I mutation on human hematopoiesis.

Blood

Mead AJ,Chowdhury O,Pecquet C,Dusa A,Woll P,Atkinson D,Burns A,Score J,Rugless M,Clifford R,Moule S,Bienz N,Vyas P,Cross N,Gale RE,Henderson S,Constantinescu SN,Schuh A,Jacobsen SE

MCD4530 was used in flow cytometry to describe a germline JAK2V617I mutation in a family with hereditary thrombocytosis and characterize the hematopoietic and signaling impact of this mutation.

Thu May 16 00:00:00 EDT 2013

Nanoparticle clearance is governed by Th1/Th2 immunity and strain background.

The Journal of clinical investigation

Jones SW,Roberts RA,Robbins GR,Perry JL,Kai MP,Chen K,Bo T,Napier ME,Ting JP,Desimone JM,Bear JE

MCD4530 was used in flow cytometry to investigate nanoparticle clearance.

Mon Jul 01 00:00:00 EDT 2013

Interleukin-6 is required for pancreatic cancer progression by promoting MAPK signaling activation and oxidative stress resistance.

Cancer research

Zhang Y,Yan W,Collins MA,Bednar F,Rakshit S,Zetter BR,Stanger BZ,Chung I,Rhim AD,di Magliano MP

MCD4530 was used in flow cytometry to demonstrate that IL-6 is required for the maintenance and progression of pancreatic cancer precursor lesions.

Tue Oct 15 00:00:00 EDT 2013

Mesenchymal stem cells are mobilized from the bone marrow during inflammation.

Frontiers in immunology

Koning JJ,Kooij G,de Vries HE,Nolte MA,Mebius RE

MCD4530 was used in flow cytometry to study the impact of mesenchymal stem cells to experimental allergic encephalomyelitis

Wed Mar 06 00:00:00 EST 2013

TIM-3 regulates innate immune cells to induce fetomaternal tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Chabtini L,Mfarrej B,Mounayar M,Zhu B,Batal I,Dakle PJ,Smith BD,Boenisch O,Najafian N,Akiba H,Yagita H,Guleria I

MCD4530 was used in flow cytometry to determine the role of TIM-3-expressing innate immune cells in the regulation of tolerance at the fetomaternal interface.

Tue Jan 01 00:00:00 EST 2013

MyD88-dependent recruitment of monocytes and dendritic cells required for protection from pulmonary Burkholderia mallei infection.

Infection and immunity

Goodyear A,Troyer R,Bielefeldt-Ohmann H,Dow S

MCD4530 was used in flow cytometry to identify key Toll-like receptor signaling pathways, effector cells, and cytokines required for protection from respiratory B. mallei infection.

Sun Jan 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T220 and B220

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Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45 Monoclonal Antibody (30-F11), Pacific Orange™

CD45 Antibody (MCD4530) in Flow

Product Details

MCD4530

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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