Invitrogen

CD45RA Monoclonal Antibody (HI100), PE, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

6 Published Figures

24 References

View all (125) CD45RA antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD45RA Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 9

CD45RA Antibody (12-0458-42) in Flow

Knockout of CD45RA was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR664203_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD45RA was performed by staining THP-1 CD45RA Knock out cells with 0.06 µg Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ (Product # 12-4732-81, yellow histogram) or 0.06 µg CD45RA Monoclonal Antibody (HI100), PE, eBioscience™ (Product # 12-0458-42, blue histogram) in the presence of Fc Receptor Binding Inhibi... View More

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Product Details

12-0458-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HI100

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10718395

Product Specific Information

Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

Applications Reported: The HI100 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Specific transcriptional programs differentiate ICOS from CD28 costimulatory signaling in human Naïve CD4⁺ T cells.

Frontiers in immunology

Gigliotti CL,Boggio E,Favero F,Incarnato D,Santoro C,Oliviero S,Rojo JM,Zucchelli S,Persichetti F,Baldanzi G,Dianzani U,Corà D

12-0458-42 was used in Flow cytometry/Cell sorting to suggest that ICOS and CD28 costimulatory signals play distinct roles during the activation of naive T cells by modulating distinct sets of immunological and immunometabolic genes.

Fri Sep 23 00:00:00 EDT 2022

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD45RA monoclonal antibody (Product # 12-0458-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Published figure using CD45RA monoclonal antibody (Product # 12-0458-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

Published figure using CD45RA monoclonal antibody (Product # 12-0458-42) in Flow Cytometry

Wed Sep 15 00:00:00 EDT 2021

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

Published figure using CD45RA monoclonal antibody (Product # 12-0458-42) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Published figure using CD45RA monoclonal antibody (Product # 12-0458-42) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

Published figure using CD45RA monoclonal antibody (Product # 12-0458-42) in Flow Cytometry

Tue Apr 28 00:00:00 EDT 2020

TARGET-Seq: A Protocol for High-Sensitivity Single-Cell Mutational Analysis and Parallel RNA Sequencing.

STAR protocols

Rodriguez-Meira A,O'Sullivan J,Rahman H,Mead AJ

12-0458 was used in Flow cytometry/Cell sorting to present a detailed step-by-step protocol for TARGET-seq, including troubleshooting tips, approaches for automation, and methods for high-throughput multiplexing of libraries.

Fri Dec 18 00:00:00 EST 2020

Unravelling Intratumoral Heterogeneity through High-Sensitivity Single-Cell Mutational Analysis and Parallel RNA Sequencing.

Molecular cell

Rodriguez-Meira A,Buck G,Clark SA,Povinelli BJ,Alcolea V,Louka E,McGowan S,Hamblin A,Sousos N,Barkas N,Giustacchini A,Psaila B,Jacobsen SEW,Thongjuea S,Mead AJ

12-0458-42 was used in Flow Cytometry to develop a method for the high-sensitivity detection of multiple mutations within single cells from both genomic and coding DNA, in parallel with unbiased whole-transcriptome analysis.

Thu Mar 21 00:00:00 EDT 2019

MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo.

Molecular medicine reports

Lv M,Li Z,Liu J,Lin F,Zhang Q,Li Z,Wang Y,Wang K,Xu Y

Published figure using CD45RA monoclonal antibody (Product # 12-0458-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Targeting enhancer switching overcomes non-genetic drug resistance in acute myeloid leukaemia.

Nature communications

Bell CC,Fennell KA,Chan YC,Rambow F,Yeung MM,Vassiliadis D,Lara L,Yeh P,Martelotto LG,Rogiers A,Kremer BE,Barbash O,Mohammad HP,Johanson TM,Burr ML,Dhar A,Karpinich N,Tian L,Tyler DS,MacPherson L,Shi J,Pinnawala N,Yew Fong C,Papenfuss AT,Grimmond SM,Dawson SJ,Allan RS,Kruger RG,Vakoc CR,Goode DL,Naik SH,Gilan O,Lam EYN,Marine JC,Prinjha RK,Dawson MA

Published figure using CD45RA monoclonal antibody (Product # 12-0458-42) in Flow Cytometry

Thu Jun 20 00:00:00 EDT 2019

Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection.

Nature

Eyquem J,Mansilla-Soto J,Giavridis T,van der Stegen SJ,Hamieh M,Cunanan KM,Odak A,Gönen M,Sadelain M

Published figure using CD45RA monoclonal antibody (Product # 12-0458-42) in Flow Cytometry

Thu Mar 02 00:00:00 EST 2017

The distribution and function of human memory T cell subsets in lung cancer.

Immunologic research

Sheng SY,Gu Y,Lu CG,Zou JY,Hong H,Wang R

Published figure using CD45RA monoclonal antibody (Product # 12-0458-42) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

Published figure using CD45RA monoclonal antibody (Product # 12-0458-42) in Flow Cytometry

Sat Jan 29 00:00:00 EST 2022

Heterogeneous leukemia stem cells in myeloid blast phase chronic myeloid leukemia.

Blood advances

Kinstrie R,Karamitros D,Goardon N,Morrison H,Hamblin M,Robinson L,Clark RE,Copland M,Vyas P

12-0458 was used in Flow cytometry/Cell sorting to study the haemopoietic hierarchy of additional mutations in blast phase chronic myeloid leukaemia, and the cellular identity of the resulting leukaemia-propagating stem cell.

Tue Dec 27 00:00:00 EST 2016

Circulating precursors of human CD1c+ and CD141+ dendritic cells.

The Journal of experimental medicine

Breton G,Lee J,Zhou YJ,Schreiber JJ,Keler T,Puhr S,Anandasabapathy N,Schlesinger S,Caskey M,Liu K,Nussenzweig MC

12-0458 was used in Flow cytometry/Cell sorting to identify a migratory precursor of dendritic cells using a novel tissue culture system.

Mon Mar 09 00:00:00 EDT 2015

Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow.

The Journal of experimental medicine

Lee J,Breton G,Oliveira TY,Zhou YJ,Aljoufi A,Puhr S,Cameron MJ,Sékaly RP,Nussenzweig MC,Liu K

12-0458 was used in Flow cytometry/Cell sorting to study the development of dendritic cells from hematopoietic stem cell progenitors using a novel culture system.

Mon Mar 09 00:00:00 EDT 2015

Skewed T-helper (Th)1/2- and Th17/T regulatory‑cell balances in patients with renal cell carcinoma.

Molecular medicine reports

Li L,Yang C,Zhao Z,Xu B,Zheng M,Zhang C,Min Z,Guo J,Rong R

12-0458 was used in Flow cytometry/Cell sorting to demonstrate that the balance of Th1 and Th2 cells is skewed towards the Th2 profile in the peripheral blood of patients with RCC.

Sun Feb 01 00:00:00 EST 2015

Programmed death-1+ T cells and regulatory T cells are enriched in tumor-involved lymph nodes and associated with aggressive features in papillary thyroid cancer.

The Journal of clinical endocrinology and metabolism

French JD,Kotnis GR,Said S,Raeburn CD,McIntyre RC,Klopper JP,Haugen BR

12-0458 was used in Flow cytometry/Cell sorting to characterise the CD4(+) T cell response to metastatic PTC.

Fri Jun 01 00:00:00 EDT 2012

The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin.

PloS one

Qi Y,Operario DJ,Georas SN,Mosmann TR

12-0458 was used in Flow cytometry/Cell sorting to collate a detailed analysis of the regulation of Amphiregulin expression by human T cell subsets.

Thu Dec 13 00:00:00 EST 2012

Proportions of CD4+ memory T cells are altered in individuals chronically infected with Schistosoma haematobium.

Scientific reports

Nausch N,Bourke CD,Appleby LJ,Rujeni N,Lantz O,Trottein F,Midzi N,Mduluza T,Mutapi F

12-0458 was used in Flow cytometry/Cell sorting to investigate the association between human helminth infection and CD4+ memory T cell proportion in individuals chronically infected with Schistosoma haematobium.

Mon Feb 25 00:00:00 EST 2013

RUNX transcription factor-mediated association of Cd4 and Cd8 enables coordinate gene regulation.

Immunity

Collins A,Hewitt SL,Chaumeil J,Sellars M,Micsinai M,Allinne J,Parisi F,Nora EP,Bolland DJ,Corcoran AE,Kluger Y,Bosselut R,Ellmeier W,Chong MM,Littman DR,Skok JA

12-0458 was used in Flow cytometry/Cell sorting to study how communication between Cd4 and Cd8 appears to enable long-range epigenetic regulation to ensure that expression of one excludes the other in mature CD4 or CD8 single-positive cells.

Fri Mar 25 00:00:00 EDT 2011

RUNX transcription factor-mediated association of Cd4 and Cd8 enables coordinate gene regulation.

Immunity

Collins A,Hewitt SL,Chaumeil J,Sellars M,Micsinai M,Allinne J,Parisi F,Nora EP,Bolland DJ,Corcoran AE,Kluger Y,Bosselut R,Ellmeier W,Chong MM,Littman DR,Skok JA

Fri Mar 25 00:00:00 EDT 2011

IL-15 transpresentation promotes both human T-cell reconstitution and T-cell-dependent antibody responses in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Huntington ND,Alves NL,Legrand N,Lim A,Strick-Marchand H,Mention JJ,Plet A,Weijer K,Jacques Y,Becker PD,Guzman C,Soussan P,Kremsdorf D,Spits H,Di Santo JP

12-0458 was used in Flow cytometry/Cell sorting to investigate the role of transpresented IL-15 on human T-cell development and homeostasis.

Tue Apr 12 00:00:00 EDT 2011

Generation of bivalent chromatin domains during cell fate decisions.

Epigenetics & chromatin

De Gobbi M,Garrick D,Lynch M,Vernimmen D,Hughes JR,Goardon N,Luc S,Lower KM,Sloane-Stanley JA,Pina C,Soneji S,Renella R,Enver T,Taylor S,Jacobsen SE,Vyas P,Gibbons RJ,Higgs DR

12-0458 was used in Flow cytometry/Cell sorting to investigate the patterns and levels of histone modifications at bivalent genes, showing the generation of bivalent chromatin domains during cell fate decisions.

Mon Jun 06 00:00:00 EDT 2011

Dendritic cells express hematopoietic prostaglandin D synthase and function as a source of prostaglandin D2 in the skin.

The American journal of pathology

Shimura C,Satoh T,Igawa K,Aritake K,Urade Y,Nakamura M,Yokozeki H

12-0458 was used in Flow cytometry/Cell sorting to determine that dendritic cells may mediate or regulate skin inflammation by releasing PGD2 in response to various stimuli.

Fri Jan 01 00:00:00 EST 2010

Naive CD4 t cell proliferation is controlled by mammalian target of rapamycin regulation of GRAIL expression.

Journal of immunology (Baltimore, Md. : 1950)

Lin JT,Lineberry NB,Kattah MG,Su LL,Utz PJ,Fathman CG,Wu L

12-0458 was used in Flow cytometry/Cell sorting to investigate the role of GRAIL in CD4 T cells, showing that proliferation is controlled by mTOR regulation of GRAIL expression.

Fri May 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T100; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45RA Monoclonal Antibody (HI100), PE, eBioscience™

CD45RA Antibody (12-0458-42) in Flow

Product Details

12-0458-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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