Invitrogen

CD45RA Monoclonal Antibody (HI100), PE-Cyanine7, eBioscience™

CD45RA Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 7

CD45RA Antibody (25-0458-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4732-81) (open histogram) or Anti-Human CD45RA PE-Cyanine7 (filled histogram). Cells in the lymphocyte (left) or monocyte (right) gate were used for analysis.

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Product Details

25-0458-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HI100

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1548774

Product Specific Information

Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

Applications Reported: This HI100 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD45RA monoclonal antibody (Product # 25-0458-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

25-0458-42 was used in Flow Cytometry to study the effects of SARS-CoV-2 virus infection on immune and metabolic functions in human cells.

Tue Jun 01 00:00:00 EDT 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

Published figure using CD45RA monoclonal antibody (Product # 25-0458-42) in Flow Cytometry

Wed Sep 15 00:00:00 EDT 2021

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

Published figure using CD45RA monoclonal antibody (Product # 25-0458-42) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

Published figure using CD45RA monoclonal antibody (Product # 25-0458-42) in Flow Cytometry

Tue Apr 28 00:00:00 EDT 2020

MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo.

Molecular medicine reports

Lv M,Li Z,Liu J,Lin F,Zhang Q,Li Z,Wang Y,Wang K,Xu Y

Published figure using CD45RA monoclonal antibody (Product # 25-0458-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Targeting enhancer switching overcomes non-genetic drug resistance in acute myeloid leukaemia.

Nature communications

Bell CC,Fennell KA,Chan YC,Rambow F,Yeung MM,Vassiliadis D,Lara L,Yeh P,Martelotto LG,Rogiers A,Kremer BE,Barbash O,Mohammad HP,Johanson TM,Burr ML,Dhar A,Karpinich N,Tian L,Tyler DS,MacPherson L,Shi J,Pinnawala N,Yew Fong C,Papenfuss AT,Grimmond SM,Dawson SJ,Allan RS,Kruger RG,Vakoc CR,Goode DL,Naik SH,Gilan O,Lam EYN,Marine JC,Prinjha RK,Dawson MA

Published figure using CD45RA monoclonal antibody (Product # 25-0458-42) in Flow Cytometry

Thu Jun 20 00:00:00 EDT 2019

Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection.

Nature

Eyquem J,Mansilla-Soto J,Giavridis T,van der Stegen SJ,Hamieh M,Cunanan KM,Odak A,Gönen M,Sadelain M

Published figure using CD45RA monoclonal antibody (Product # 25-0458-42) in Flow Cytometry

Thu Mar 02 00:00:00 EST 2017

Squamous Cell Tumors Recruit γδ T Cells Producing either IL17 or IFNγ Depending on the Tumor Stage.

Cancer immunology research

Lo Presti E,Toia F,Oieni S,Buccheri S,Turdo A,Mangiapane LR,Campisi G,Caputo V,Todaro M,Stassi G,Cordova A,Moschella F,Rinaldi G,Meraviglia S,Dieli F

25-0458 was used in Flow cytometry/Cell sorting to provide insights into the functions of tumor-infiltrating γδ T cells.

Mon May 01 00:00:00 EDT 2017

The distribution and function of human memory T cell subsets in lung cancer.

Immunologic research

Sheng SY,Gu Y,Lu CG,Zou JY,Hong H,Wang R

Published figure using CD45RA monoclonal antibody (Product # 25-0458-42) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

Published figure using CD45RA monoclonal antibody (Product # 25-0458-42) in Flow Cytometry

Sat Jan 29 00:00:00 EST 2022

Distinctive features of tumor-infiltrating γδ T lymphocytes in human colorectal cancer.

Oncoimmunology

Meraviglia S,Lo Presti E,Tosolini M,La Mendola C,Orlando V,Todaro M,Catalano V,Stassi G,Cicero G,Vieni S,Fourniè JJ,Dieli F

25-0458 was used in Flow cytometry/Cell sorting to characterise features of γδ T cells in colorectal cancer, normal colon tissue and peripheral blood, and correlate their levels with clinicopathologic hallmarks.

Tue Jan 12 00:00:00 EST 2021

Early Rise of Blood T Follicular Helper Cell Subsets and Baseline Immunity as Predictors of Persisting Late Functional Antibody Responses to Vaccination in Humans.

PloS one

Spensieri F,Siena E,Borgogni E,Zedda L,Cantisani R,Chiappini N,Schiavetti F,Rosa D,Castellino F,Montomoli E,Bodinham CL,Lewis DJ,Medini D,Bertholet S,Del Giudice G

25-0458 was used in Flow cytometry/Cell sorting to identify, in human peripheral blood, early T follicular helper cell subset(s) predicting not only the rise but also the long term persistence of functional antibody titers after seasonal influenza vaccination.

Mon Jul 24 00:00:00 EDT 2017

Tumor-infiltrating Tim-3⁺ T cells proliferate avidly except when PD-1 is co-expressed: Evidence for intracellular cross talk.

Oncoimmunology

Li J,Shayan G,Avery L,Jie HB,Gildener-Leapman N,Schmitt N,Lu BF,Kane LP,Ferris RL

25-0458 was used in Flow cytometry/Cell sorting to investigate the phenotypic and functional characteristics of T cells with differential expression of PD-1 and Tim-3.

Tue Jan 12 00:00:00 EST 2021

Increased CD226 Expression on CD8+ T Cells Is Associated with Upregulated Cytokine Production and Endothelial Cell Injury in Patients with Systemic Sclerosis.

Journal of immunology (Baltimore, Md. : 1950)

Ayano M,Tsukamoto H,Kohno K,Ueda N,Tanaka A,Mitoma H,Akahoshi M,Arinobu Y,Niiro H,Horiuchi T,Akashi K

25-0458 was used in Flow cytometry/Cell sorting to indicate that upregulated CD226 expression on CD8(+) T cells reflects disease severity and is involved in systemic sclerosis pathogenesis.

Sat Aug 01 00:00:00 EDT 2015

The upregulation of LAG-3 on T cells defines a subpopulation with functional exhaustion and correlates with disease progression in HIV-infected subjects.

Journal of immunology (Baltimore, Md. : 1950)

Tian X,Zhang A,Qiu C,Wang W,Yang Y,Qiu C,Liu A,Zhu L,Yuan S,Hu H,Wang W,Wei Q,Zhang X,Xu J

25-0458 was used in Flow cytometry/Cell sorting to study the role of LAG-3 on T cell functionality during persistent HIV-1 infection.

Wed Apr 15 00:00:00 EDT 2015

Effect of chorioamnionitis on regulatory T cells in moderate/late preterm neonates.

Human immunology

Rueda CM,Wells CB,Gisslen T,Jobe AH,Kallapur SG,Chougnet CA

25-0458 was used in Flow cytometry/Cell sorting to show that relative to term, prematurity and chorioamnionitis decrease Treg cell functionality without affecting their frequency or phenotype.

Thu Jan 01 00:00:00 EST 2015

Anti-angiogenic activity of VXM01, an oral T-cell vaccine against VEGF receptor 2, in patients with advanced pancreatic cancer: A randomized, placebo-controlled, phase 1 trial.

Oncoimmunology

Schmitz-Winnenthal FH,Hohmann N,Niethammer AG,Friedrich T,Lubenau H,Springer M,Breiner KM,Mikus G,Weitz J,Ulrich A,Buechler MW,Pianka F,Klaiber U,Diener M,Leowardi C,Schimmack S,Sisic L,Keller AV,Koc R,Springfeld C,Knebel P,Schmidt T,Ge Y,Bucur M,Stamova S,Podola L,Haefeli WE,Grenacher L,Beckhove P

25-0458 was used in Flow cytometry/Cell sorting to demonstrate that VXM01 elicits a productive, complex VEGFR2 specific T effector cell response in patients with pancreatic cancer.

Wed Apr 01 00:00:00 EDT 2015

Humanized mice, a new model to study the influence of drug treatment on neonatal sepsis.

Infection and immunity

Ernst W,Zimara N,Hanses F,Männel DN,Seelbach-Göbel B,Wege AK

25-0458 was used in Flow cytometry/Cell sorting to investigate the use of humanised mice as a model of GBS-induced sepsis.

Wed May 01 00:00:00 EDT 2013

Cytotoxic response persists in subjects treated for tuberculosis decades ago.

BMC infectious diseases

Savolainen LE,Koskivirta P,Kantele A,Valleala H,Pusa L,Tuompo R,Westerlund-Wikström B,Tuuminen T

25-0458 was used in Flow cytometry/Cell sorting to evaluate the influence of various anti-TB therapeutic schemes on the immunological profiles of residual cytotoxic T lymphocytes.

Thu Dec 05 00:00:00 EST 2013

Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions.

PLoS pathogens

Qiu Y,Chen J,Liao H,Zhang Y,Wang H,Li S,Luo Y,Fang D,Li G,Zhou B,Shen L,Chen CY,Huang D,Cai J,Cao K,Jiang L,Zeng G,Chen ZW

25-0458 was used in Flow cytometry/Cell sorting to uncover a previously unknown mechanism for T-cell immune responses regulated by Tim-3, which may have implications for potential immune intervention in TB.

Fri Apr 19 00:00:00 EDT 2013

Leishmania major infection in humanized mice induces systemic infection and provokes a nonprotective human immune response.

PLoS neglected tropical diseases

Wege AK,Florian C,Ernst W,Zimara N,Schleicher U,Hanses F,Schmid M,Ritter U

25-0458 was used in Flow cytometry/Cell sorting to describe Leishmania major infection in humanised mice for the first time, characterising disease development and the induction of the human immune response.

Thu Nov 01 00:00:00 EDT 2012

Human CD8⁺ and CD4⁺ T cell memory to lymphocytic choriomeningitis virus infection.

Journal of virology

Kotturi MF,Swann JA,Peters B,Arlehamn CL,Sidney J,Kolla RV,James EA,Akondy RS,Ahmed R,Kwok WW,Buchmeier MJ,Sette A

25-0458 was used in Flow cytometry/Cell sorting to analyse the T cell response to acute lymphocytic choriomeningitis virus infection.

Tue Nov 01 00:00:00 EDT 2011

CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

Landrigan A,Wong MT,Utz PJ

25-0458 was used in Flow cytometry/Cell sorting to study the ability of oligodeoxynucleotides to activate and polarise T cells.

Thu Sep 15 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T100; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45RA Monoclonal Antibody (HI100), PE-Cyanine7, eBioscience™

CD45RA Antibody (25-0458-42) in Flow

Product Details

25-0458-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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