Invitrogen

CD45RO Monoclonal Antibody (UCHL1)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

24 References

View all (42) CD45RO antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Advanced Verification (1)

CD45RO Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

CD45RO Antibody (MA1-19452) in Flow

Separation of human CD45R0 positive lymphocytes (red-filled) from CD45R0 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD45R0 (UCHL1) purified Monoclonal antibody (Product # MA1-19452) (concentration in sample 1 µg/mL, GAM APC).

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Product Details

MA1-19452

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

10 µg/mL

View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

UCHL1

Immunogen

Human IL-2 dependent T cells.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1072483

Product Specific Information

This antibody recognizes an extracellular epitope of CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes.

Pretreatment note: MA1-19452 does not require protein digestion pretreatment of paraffin sections and does not require antigen retrieval using heat treatment prior to staining of paraffin sections. A suggested positive control for this product is tonsil tissue.

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative, qualitative and spatial analysis of lymphocyte infiltration in periampullary and pancreatic adenocarcinoma.

International journal of cancer

Lundgren S,Elebro J,Heby M,Nodin B,Leandersson K,Micke P,Jirström K,Mezheyeuski A

MA1-19452 was used in Immunohistochemistry to demonstrate that the composition and clinical impact of immune infiltrates in periampullary adenocarcinoma differ by morphological type as well as localization.

Mon Jun 15 00:00:00 EDT 2020

Prognostic Interactions between FAP+ Fibroblasts and CD8a+ T Cells in Colon Cancer.

Cancers

Herrera M,Mezheyeuski A,Villabona L,Corvigno S,Strell C,Klein C,Hölzlwimmer G,Glimelius B,Masucci G,Sjöblom T,Östman A

MA1-19452 was used in Immunohistochemistry to present findings relevant for the ongoing efforts to improve the prognostic performance of CD8-related markers and should be followed by additional validation studies.

Tue Nov 03 00:00:00 EST 2020

The absence of HLA class I expression in non-small cell lung cancer correlates with the tumor tissue structure and the pattern of T cell infiltration.

International journal of cancer

Perea F,Bernal M,Sánchez-Palencia A,Carretero J,Torres C,Bayarri C,Gómez-Morales M,Garrido F,Ruiz-Cabello F

MA119452 was used in immunohistochemistry - frozen section to characterize HLA class I-positive and negative tumors

Wed Feb 15 00:00:00 EST 2017

CD103+ intraepithelial T cells in high-grade serous ovarian cancer are phenotypically diverse TCRαβ+ CD8αβ+ T cells that can be targeted for cancer immunotherapy.

Oncotarget

Komdeur FL,Wouters MC,Workel HH,Tijans AM,Terwindt AL,Brunekreeft KL,Plat A,Klip HG,Eggink FA,Leffers N,Helfrich W,Samplonius DF,Bremer E,Wisman GB,Daemen T,Duiker EW,Hollema H,Nijman HW,de Bruyn M

MA119452 was used in flow cytometry to define the contribution of CD103+ tumor-infiltrating lymphocytes to high-grade serous epithelial ovarian cancer

Tue Nov 15 00:00:00 EST 2016

CD30⁺ extranodal natural killer/T-cell lymphoma mimicking phlegmonous myositis: A case report.

Oncology letters

Yang YJ,Li YX,Liu YB,Yang M,Liu K

MA1-19452 was used in immunohistochemistry (paraffin) to report on a case of CD30-positive extranodal natural killer/T-cell lymphoma.

Thu May 01 00:00:00 EDT 2014

A giant primary sclerosing lipogranuloma of the scrotum.

Turk patoloji dergisi

Mungan S,Karagüzel E,Turan C,Reıs A

MA1-19452 was used in immunohistochemistry to report on a case of giant primary sclerosing lipogranuloma of the scrotum

Tue Jul 08 00:00:00 EDT 2014

IL-21 can supplement suboptimal Lck-independent MAPK activation in a STAT-3-dependent manner in human CD8(+) T cells.

Journal of immunology (Baltimore, Md. : 1950)

Imataki O,Ansén S,Tanaka M,Butler MO,Berezovskaya A,Milstein MI,Kuzushima K,Nadler LM,Hirano N

MA1-19452 was used in flow cytometry to investigate the role of CD8 and antigen in proliferation of CTLs.

Wed Feb 15 00:00:00 EST 2012

The significance of multiplex PCR/heteroduplex analysis-based TCR-γ gene rearrangement combined with laser-capture microdissection in the diagnosis of early mycosis fungoides.

Journal of cutaneous pathology

Yang H,Xu C,Tang Y,Wan C,Liu W,Wang L

MA1-19452 was used in immunohistochemistry (paraffin) to study the diagnostic value for detecting early mycosis fungoides of a combination of multiplex PCR analyis of TCRgamma gene rearrangements and laser-capture microdissection.

Thu Mar 01 00:00:00 EST 2012

Serous effusion cytology of extranodal natural killer/T-cell lymphoma.

Cytopathology : official journal of the British Society for Clinical Cytology

Su XY,Huang J,Jiang Y,Tang Y,Li GD,Liu WP

MA1-19452 was used in flow cytometry to characterize extranodal natural killer/T-cell lymphoma, nasal type using patient samples.

Sun Apr 01 00:00:00 EDT 2012

Effector Memory T cells Are Associated With Atherosclerosis in Humans and Animal Models.

Journal of the American Heart Association

Ammirati E,Cianflone D,Vecchio V,Banfi M,Vermi AC,De Metrio M,Grigore L,Pellegatta F,Pirillo A,Garlaschelli K,Manfredi AA,Catapano AL,Maseri A,Palini AG,Norata GD

MA1-19452 was used in flow cytometry to study the relationship between circulating CD4+ T-cell subsets and atherosclerosis

Wed Feb 01 00:00:00 EST 2012

Axonal projections of mechanoreceptive dorsal root ganglion neurons depend on Ret.

Development (Cambridge, England)

Honma Y,Kawano M,Kohsaka S,Ogawa M

MA1-19452 was used in immunohistochemistry to study the role of Ret in the neurturin-mediated formation of precise axonal projections in the central nervous system

Thu Jul 01 00:00:00 EDT 2010

A patient with clinicopathologic features of small plaque parapsoriasis presenting later with plaque-stage mycosis fungoides: report of a case and comparative retrospective study of 27 cases of "nonprogressive" small plaque parapsoriasis.

Journal of the American Academy of Dermatology

Belousova IE,Vanecek T,Samtsov AV,Michal M,Kazakov DV

MA1-19452 was used in immunohistochemistry to report on a case of small plaque parapsoriasis with plaque-stage mycosis fungoides

Mon Sep 01 00:00:00 EDT 2008

Hyperplasia of hair follicles and other adnexal structures in cutaneous lymphoproliferative disorders: a study of 53 cases, including so-called pseudolymphomatous folliculitis and overt lymphomas.

The American journal of surgical pathology

Kazakov DV,Belousova IE,Kacerovska D,Sima R,Vanecek T,Vazmitel M,Pizinger K,Michal M

MA1-19452 was used in immunohistochemistry to study 53 cases of cutaneous lymphoproliferative disorders

Wed Oct 01 00:00:00 EDT 2008

Natural regulatory T cells: number and function are normal in the majority of patients with lupus nephritis.

Clinical and experimental immunology

Yates J,Whittington A,Mitchell P,Lechler RI,Lightstone L,Lombardi G

MA1-19452 was used in flow cytometry to examine the frequency, phenotype, and function of T regulatory cells in patients with systemic lupus erythematosus and inactive disease

Tue Jul 01 00:00:00 EDT 2008

Up-regulation of expression of the ubiquitin carboxyl-terminal hydrolase L1 gene in human airway epithelium of cigarette smokers.

Cancer research

Carolan BJ,Heguy A,Harvey BG,Leopold PL,Ferris B,Crystal RG

MA1-19452 was used in immunocytochemistry to investigate the expression of carboxyl-terminal hydrolyase L1 gene in human airway epithelium of cigarette smokers

Wed Nov 15 00:00:00 EST 2006

Monocyte-derived human macrophages mediate anergy in allogeneic T cells and induce regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Hoves S,Krause SW,Schütz C,Halbritter D,Schölmerich J,Herfarth H,Fleck M

MA1-19452 was used in flow cytometry to assess the T cell-stimulatory capacity of mature dendritic cells and macrophages using a model of allogeneic MLR

Tue Aug 15 00:00:00 EDT 2006

Primary cutaneous histiocyte and neutrophil-rich CD30+ and CD56+ anaplastic large-cell lymphoma with prominent angioinvasion and nerve involvement in the forehead and scalp of an immunocompetent woman.

Journal of cutaneous pathology

Boudova L,Kazakov DV,Jindra P,Sima R,Vanecek T,Kuntscher V,Vera V,Bouda J,Michal M

MA1-19452 was used in immunohistochemistry to report on a case of utaneous lymphoma of the head co-expressing CD56 and CD30

Tue Aug 01 00:00:00 EDT 2006

CIK cells from patients with HCC possess strong cytotoxicity to multidrug-resistant cell line Bel-7402/R.

World journal of gastroenterology

Zhang YS,Yuan FJ,Jia GF,Zhang JF,Hu LY,Huang L,Wang J,Dai ZQ

MA1-19452 was used in immunohistochemistry to investigate the effectiveness of cytokine-induced killer cell treatment in patient with hepatocellular carcinoma

Tue Jun 14 00:00:00 EDT 2005

Cutaneous lymphoid hyperplasia and other lymphoid infiltrates of the breast nipple: a retrospective clinicopathologic study of fifty-six patients.

The American Journal of dermatopathology

Boudova L,Kazakov DV,Sima R,Vanecek T,Torlakovic E,Lamovec J,Kutzner H,Szepe P,Plank L,Bouda J,Hes O,Mukensnabl P,Michal M

MA1-19452 was used in immunohistochemistry to study the clinicopathological spectrum of lymphoproliferations and other lymphoid infiltration of the breast nipple

Sat Oct 01 00:00:00 EDT 2005

T-cell-rich B-cell lymphoma: a clinicopathologic study of 21 cases and comparison with 43 cases of diffuse large B-cell lymphoma.

Leukemia research

Aki H,Tuzuner N,Ongoren S,Baslar Z,Soysal T,Ferhanoglu B,Sahinler I,Aydin Y,Ulku B,Aktuglu G

MA1-19452 was used in immunohistochemistry to compare the clinocopathology of T-cell rich B-cell lymphoma and diffuse large B-cell lymphoma

Mon Mar 01 00:00:00 EST 2004

Chemokine receptor CCR4 on CD4+ T cells in juvenile rheumatoid arthritis synovial fluid defines a subset of cells with increased IL-4:IFN-gamma mRNA ratios.

Journal of immunology (Baltimore, Md. : 1950)

Thompson SD,Luyrink LK,Graham TB,Tsoras M,Ryan M,Passo MH,Glass DN

MA1-19452 was used in flow cytometry to elucidate the mechanisms that promote recruitment and survival of T cells within the pediatric inflamed joint.

Fri Jun 01 00:00:00 EDT 2001

Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages.

Proceedings of the National Academy of Sciences of the United States of America

Lee B,Sharron M,Montaner LJ,Weissman D,Doms RW

MA1-19452 was used in flow cytometry to determine the number of CD4, CCR5, and CXCR4 antibody-binding sites on various T cells and macrophages.

Tue Apr 27 00:00:00 EDT 1999

CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells.

Journal of immunology (Baltimore, Md. : 1950)

Kim CH,Pelus LM,White JR,Applebaum E,Johanson K,Broxmeyer HE

MA1-19452 was used in flow cytometry to determine the functional properties of CK beta-11/MIP-3 beta/ELC.

Sun Mar 01 00:00:00 EST 1998

Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.

Immunology

Smith SH,Brown MH,Rowe D,Callard RE,Beverley PC

MA1-19452 was used in flow cytometry, immunohistochemistry - frozen section, and immunoprecipitation to characterize the monoclonal antibody UCHL1.

Thu May 01 00:00:00 EDT 1986

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45RO Monoclonal Antibody (UCHL1)

CD45RO Antibody (MA1-19452) in Flow

Product Details

MA1-19452

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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