Invitrogen

CD47 Monoclonal Antibody (B6H12), FITC, eBioscience™

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

21 Published Figures

19 References

View all (156) CD47 antibodies

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CD47 Antibody (11-0479-42) in Flow

Normal human peripheral blood cells were stained with Mouse IgG1 kappa Isotype Control, FITC (Product # 11-4714-82) (blue histogram) or CD47 Monoclonal Antibody, FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

CD47 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD47 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

CD47 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD47 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD47 Antibody — Figure 8 GMP-grade iExosomes stability. ( A ) Comparison of the number of MSC exosomes, quantified by NanoSight, prior to freezing and after thawing of frozen exosomes ( n = 4 distinct exosomes aliquots). The times listed refer to the times that exosomes were stored for at -80degC. ( B ) Particle size distribution analysis of fresh and frozen (45 days) and then thawed MSC exosomes by NanoSight. ( C ) TEM of MSC exosomes, prior to freezing (fresh) and after freezing (45 days) and thawing. Scale bar: 100 nm. ( D ) Representative histogram of flow cytometry analyses of exosomal markers (CD9, CD63, CD81, CD47) on fresh versus freeze (45 days) and thaw MSC exosomes. Numbers represent the percentage of positive beads (gray, isotype control). ( E and F ) Representative dot plot of flow cytometry analyses ( E ) and quantification ( F ) of apoptosis in Panc-1 cells induced by MSCs siKras G12D iExo (48 hours following iExo treatment), comparing the efficacy of freeze (3 months) and thaw iExosomes that were allowed to incubate for 3, 6, and 24 hours and 2, 3, 4, and 5 days at room temperature (RT) or 4degC ( n = 2-3 independent experiments). Numbers represent the percentage of positive cells. One-way ANOVA compared with fresh exosomes. ( G ) KRAS G12D transcript levels in Panc-1 cells treated with MSCs siKras G12D iExo after 3 hours, comparing the efficacy of freeze (3 months) and thaw of iExosomes, under the listed conditions ( n = 3 independent experiments, 1 {TM}

Product Details

11-0479-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 12 publications 12 publications

Inhibition Assays (IA)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B6H12

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2043842

Product Specific Information

Description: The monoclonal antibody B6H12 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small alternatively spliced cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with beta3 integrins CD51/CD61 and CD41/CD61. Furthermore this interaction can mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages. CD47 is the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Recently stimulation of CD47 by monoclonal antibody has been shown to induce CD4+CD25- suppressive activity also increasing expression of Foxp3. Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.

This antibody has been reported to have neutralizing activity.

Applications Reported: This B6H12 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This B6H12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Altered Membrane Expression and Function of CD11b Play a Role in the Immunosuppressive Effects of Morphine on Macrophages at the Nanomolar Level.

Pharmaceuticals (Basel, Switzerland)

Yu PC,Hao CY,Fan YZ,Liu D,Qiao YF,Yao JB,Li CZ,Yu Y

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2023

Melanophages give rise to hyperreflective foci in AMD, a disease-progression marker.

Journal of neuroinflammation

Augustin S,Lam M,Lavalette S,Verschueren A,Blond F,Forster V,Przegralek L,He Z,Lewandowski D,Bemelmans AP,Picaud S,Sahel JA,Mathis T,Paques M,Thuret G,Guillonneau X,Delarasse C,Sennlaub F

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Inhibition Assays

Wed Feb 08 00:00:00 EST 2023

CD47 Blockade Accelerates Blood Clearance and Alleviates Early Brain Injury After Experimental Subarachnoid Hemorrhage.

Frontiers in immunology

Xu CR,Li JR,Jiang SW,Wan L,Zhang X,Xia L,Hua XM,Li ST,Chen HJ,Fu XJ,Jing CH

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Inhibition Assays

Mon May 02 00:00:00 EDT 2022

Schweinfurthin induces ICD without ER stress and caspase activation.

Oncoimmunology

Zhang R,Neighbors JD,Schell TD,Hohl RJ

11-0479-42 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which MeSG treatment induces immunogenic cell death (ICD).

Tue Aug 09 00:00:00 EDT 2022

Schweinfurthin induces ICD without ER stress and caspase activation.

Oncoimmunology

Zhang R,Neighbors JD,Schell TD,Hohl RJ

11-0479-42 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which MeSG treatment induces immunogenic cell death (ICD).

Tue Aug 09 00:00:00 EDT 2022

CD24: A Marker for an Extended Expansion Potential of Urothelial Cancer Cell Organoids In Vitro?

International journal of molecular sciences

Geng R,Harland N,Montes-Mojarro IA,Fend F,Aicher WK,Stenzl A,Amend B

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Immunohistochemistry (Paraffin)

Fri May 13 00:00:00 EDT 2022

Anti-CD47 antibody synergizes with cisplatin against laryngeal cancer by enhancing phagocytic ability of macrophages.

Clinical and experimental immunology

Wang J,Zhang H,Yin X,Bian Y

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

LSD1 silencing contributes to enhanced efficacy of anti-CD47/PD-L1 immunotherapy in cervical cancer.

Cell death & disease

Xu S,Wang X,Yang Y,Li Y,Wu S

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Flow Cytometry

Wed Mar 17 00:00:00 EDT 2021

Exosome-mediated delivery of CRISPR/Cas9 for targeting of oncogenic Kras^G12D in pancreatic cancer.

Life science alliance

McAndrews KM,Xiao F,Chronopoulos A,LeBleu VS,Kugeratski FG,Kalluri R

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity.

Nature communications

von Roemeling CA,Wang Y,Qie Y,Yuan H,Zhao H,Liu X,Yang Z,Yang M,Deng W,Bruno KA,Chan CK,Lee AS,Rosenfeld SS,Yun K,Johnson AJ,Mitchell DA,Jiang W,Kim BYS

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Immunohistochemistry

Fri Mar 20 00:00:00 EDT 2020

CD47 prevents the elimination of diseased fibroblasts in scleroderma.

JCI insight

Lerbs T,Cui L,King ME,Chai T,Muscat C,Chung L,Brown R,Rieger K,Shibata T,Wernig G

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Immunocytochemistry

Thu Aug 20 00:00:00 EDT 2020

Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment.

Cell death & disease

Guillon J,Petit C,Moreau M,Toutain B,Henry C,Roché H,Bonichon-Lamichhane N,Salmon JP,Lemonnier J,Campone M,Verrièle V,Lelièvre E,Guette C,Coqueret O

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Flow Cytometry

Wed Feb 27 00:00:00 EST 2019

Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment.

Cell death & disease

Guillon J,Petit C,Moreau M,Toutain B,Henry C,Roché H,Bonichon-Lamichhane N,Salmon JP,Lemonnier J,Campone M,Verrièle V,Lelièvre E,Guette C,Coqueret O

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Flow Cytometry

Wed Feb 27 00:00:00 EST 2019

Generation and testing of clinical-grade exosomes for pancreatic cancer.

JCI insight

Mendt M,Kamerkar S,Sugimoto H,McAndrews KM,Wu CC,Gagea M,Yang S,Blanko EVR,Peng Q,Ma X,Marszalek JR,Maitra A,Yee C,Rezvani K,Shpall E,LeBleu VS,Kalluri R

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Flow Cytometry

Thu Apr 19 00:00:00 EDT 2018

IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells.

Scientific reports

Hain A,Krämer M,Linka RM,Nakhaei-Rad S,Ahmadian MR,Häussinger D,Borkhardt A,Münk C

Fri Feb 16 00:00:00 EST 2018

CD47 promotes ovarian cancer progression by inhibiting macrophage phagocytosis.

Oncotarget

Liu R,Wei H,Gao P,Yu H,Wang K,Fu Z,Ju B,Zhao M,Dong S,Li Z,He Y,Huang Y,Yao Z

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Flow Cytometry

Tue Jun 13 00:00:00 EDT 2017

CD47 promotes ovarian cancer progression by inhibiting macrophage phagocytosis.

Oncotarget

Liu R,Wei H,Gao P,Yu H,Wang K,Fu Z,Ju B,Zhao M,Dong S,Li Z,He Y,Huang Y,Yao Z

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Flow Cytometry

Tue Jun 13 00:00:00 EDT 2017

CD47 is an adverse prognostic factor and a therapeutic target in gastric cancer.

Cancer medicine

Yoshida K,Tsujimoto H,Matsumura K,Kinoshita M,Takahata R,Matsumoto Y,Hiraki S,Ono S,Seki S,Yamamoto J,Hase K

11-0479 was used in Flow cytometry/Cell sorting to demonstrate that higher expressions of CD44 and CD47 in gastric cancer cells correlate with higher proliferation and tumorigenicity.

Tue Sep 01 00:00:00 EDT 2015

Augmentation of antitumor immunity by fusions of ethanol-treated tumor cells and dendritic cells stimulated via dual TLRs through TGF-β1 blockade and IL-12p70 production.

PloS one

Koido S,Homma S,Okamoto M,Namiki Y,Takakura K,Takahara A,Odahara S,Tsukinaga S,Yukawa T,Mitobe J,Matsudaira H,Nagatsuma K,Kajihara M,Uchiyama K,Arihiro S,Imazu H,Arakawa H,Kan S,Hayashi K,Komita H,Kamata Y,Ito M,Hara E,Ohkusa T,Gong J,Tajiri H

11-0479 was used in Flow cytometry/Cell sorting to illustrate the synergy between ethanol-treated whole tumor cells and dual TLRs-stimulated dendritic cells in inducing augmented CTL responses in vitro by fusion cell preparations.

Tue Feb 18 00:00:00 EST 2014

Cells expressing the C/EBPbeta isoform, LIP, engulf their neighbors.

PloS one

Abreu M,Sealy L

Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Flow Cytometry

Thu Apr 11 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody 1D8; Antigenic surface determinant protein OA3; CD47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; Leukocyte surface antigen CD47; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47

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Gene Aliases: CD47; IAP; MER6; OA3

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UniProt ID: (Human) Q08722

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Entrez Gene ID: (Human) 961

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Function(s)

protein binding thrombospondin receptor activity cell adhesion molecule

Process(es)

cell adhesion integrin-mediated signaling pathway opsonization positive regulation of cell proliferation response to bacterium positive regulation of cell-cell adhesion extracellular matrix organization positive regulation of inflammatory response positive regulation of phagocytosis positive regulation of T cell activation leukocyte migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD47 Monoclonal Antibody (B6H12), FITC, eBioscience™

CD47 Antibody (11-0479-42) in Flow

Product Details

11-0479-42

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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