Invitrogen

CD47 Monoclonal Antibody (B6H12), eBioscience™

CD47 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD47 Antibody (14-0479-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human placenta stained with 40 µg/mL Anti-Human CD47 Purified followed by Anti-Mouse IgG Biotin, Streptavidin Alkaline Phosphatase, and Fast Red. Nuclei are counterstained with hematoxylin.

CD47 Antibody in Immunocytochemistry (ICC/IF)

CD47 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD47 Antibody in Immunohistochemistry (IHC)

CD47 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

Product Image Gallery

Product Details

14-0479-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test

View 16 publications 16 publications

Immunoprecipitation (IP)

Assay-Dependent

Neutralization (Neu)

View 2 publications 2 publications

Inhibition Assays (IA)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

B6H12

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_837143

Product Specific Information

Description: The monoclonal antibody B6H12 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small alternatively spliced cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with beta3 integrins CD51/CD61 and CD41/CD61. Furthermore this interaction can mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages. CD47 is the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Recently stimulation of CD47 by monoclonal antibody has been shown to induce CD4+CD25- suppressive activity also increasing expression of Foxp3. Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.

This antibody has been reported to have neutralizing activity.

Applications Reported: This B6H12 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This B6H12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The B6H12 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival and can be used at less than or equal to 40 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Anti-CD47 Antibody Enhances the Efficacy of Chemotherapy in Patients with Gastric Cancer Liver Metastasis.

Journal of Cancer

Liu Z,Chen H,Ta N,Shi Z,Zhan L,Han T,Zhang J,Chang X,Yin K,Nie M

14-0479-82 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, In Vivo to demonstrate that tumor-derived exosomes are involved in GCLM progression, targeting CD47 inhibits gastric cancer tumorigenesis, and a combination of anti-CD47 antibodies and 5-Fu shows potential for treating GCLM.

Fri Mar 03 00:00:00 EST 2023

Altered Membrane Expression and Function of CD11b Play a Role in the Immunosuppressive Effects of Morphine on Macrophages at the Nanomolar Level.

Pharmaceuticals (Basel, Switzerland)

Yu PC,Hao CY,Fan YZ,Liu D,Qiao YF,Yao JB,Li CZ,Yu Y

Published figure using CD47 monoclonal antibody (Product # 14-0479-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2023

Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.

Nature communications

Chou CW,Hung CN,Chiu CH,Tan X,Chen M,Chen CC,Saeed M,Hsu CW,Liss MA,Wang CM,Lai Z,Alvarez N,Osmulski PA,Gaczynska ME,Lin LL,Ortega V,Kirma NB,Xu K,Liu Z,Kumar AP,Taverna JA,Velagaleti GVN,Chen CL,Zhang Z,Huang TH

14-0479-82 was used in Flow Cytometry to report that the reverse signaling using CD47 as a receptor additionally enhances a pro-survival function of prostate cancer cells under phagocytic attack.

Tue Oct 17 00:00:00 EDT 2023

Melanophages give rise to hyperreflective foci in AMD, a disease-progression marker.

Journal of neuroinflammation

Augustin S,Lam M,Lavalette S,Verschueren A,Blond F,Forster V,Przegralek L,He Z,Lewandowski D,Bemelmans AP,Picaud S,Sahel JA,Mathis T,Paques M,Thuret G,Guillonneau X,Delarasse C,Sennlaub F

14-0479-82 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that Cd47 expression in human RPE declines with age and in AMD, which likely participates in melanophage formation and RPE decline.

Wed Feb 08 00:00:00 EST 2023

Melanophages give rise to hyperreflective foci in AMD, a disease-progression marker.

Journal of neuroinflammation

Augustin S,Lam M,Lavalette S,Verschueren A,Blond F,Forster V,Przegralek L,He Z,Lewandowski D,Bemelmans AP,Picaud S,Sahel JA,Mathis T,Paques M,Thuret G,Guillonneau X,Delarasse C,Sennlaub F

Wed Feb 08 00:00:00 EST 2023

CD47 Blockade Accelerates Blood Clearance and Alleviates Early Brain Injury After Experimental Subarachnoid Hemorrhage.

Frontiers in immunology

Xu CR,Li JR,Jiang SW,Wan L,Zhang X,Xia L,Hua XM,Li ST,Chen HJ,Fu XJ,Jing CH

Published figure using CD47 monoclonal antibody (Product # 14-0479-82) in Inhibition Assays

Mon May 02 00:00:00 EDT 2022

Schweinfurthin induces ICD without ER stress and caspase activation.

Oncoimmunology

Zhang R,Neighbors JD,Schell TD,Hohl RJ

Published figure using CD47 monoclonal antibody (Product # 14-0479-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2022

Anti-CD47 antibody synergizes with cisplatin against laryngeal cancer by enhancing phagocytic ability of macrophages.

Clinical and experimental immunology

Wang J,Zhang H,Yin X,Bian Y

Published figure using CD47 monoclonal antibody (Product # 14-0479-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

LSD1 silencing contributes to enhanced efficacy of anti-CD47/PD-L1 immunotherapy in cervical cancer.

Cell death & disease

Xu S,Wang X,Yang Y,Li Y,Wu S

Published figure using CD47 monoclonal antibody (Product # 14-0479-82) in Flow Cytometry

Wed Mar 17 00:00:00 EDT 2021

Exosome-mediated delivery of CRISPR/Cas9 for targeting of oncogenic Kras^G12D in pancreatic cancer.

Life science alliance

McAndrews KM,Xiao F,Chronopoulos A,LeBleu VS,Kugeratski FG,Kalluri R

Published figure using CD47 monoclonal antibody (Product # 14-0479-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker.

Nature cell biology

Kugeratski FG,Hodge K,Lilla S,McAndrews KM,Zhou X,Hwang RF,Zanivan S,Kalluri R

14-0479-82 was used in Flow cytometry/Cell sorting to provide a comprehensive quantitative atlas of core proteins ubiquitous to exosomes that can serve as a resource for the scientific community.

Tue Jun 01 00:00:00 EDT 2021

CD47 prevents the elimination of diseased fibroblasts in scleroderma.

JCI insight

Lerbs T,Cui L,King ME,Chai T,Muscat C,Chung L,Brown R,Rieger K,Shibata T,Wernig G

14-0479 was used in Immunohistochemistry to.

Thu Aug 20 00:00:00 EDT 2020

CD47 prevents the elimination of diseased fibroblasts in scleroderma.

JCI insight

Lerbs T,Cui L,King ME,Chai T,Muscat C,Chung L,Brown R,Rieger K,Shibata T,Wernig G

14-0479 was used in Immunohistochemistry to.

Thu Aug 20 00:00:00 EDT 2020

Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity.

Nature communications

von Roemeling CA,Wang Y,Qie Y,Yuan H,Zhao H,Liu X,Yang Z,Yang M,Deng W,Bruno KA,Chan CK,Lee AS,Rosenfeld SS,Yun K,Johnson AJ,Mitchell DA,Jiang W,Kim BYS

14-0479 was used in Immunohistochemistry to show stimulation of glioma cell phagocytosis by blockage of anti-phagocytosis molecule CD47 is an ineffective process.

Fri Mar 20 00:00:00 EDT 2020

Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity.

Nature communications

von Roemeling CA,Wang Y,Qie Y,Yuan H,Zhao H,Liu X,Yang Z,Yang M,Deng W,Bruno KA,Chan CK,Lee AS,Rosenfeld SS,Yun K,Johnson AJ,Mitchell DA,Jiang W,Kim BYS

14-0479 was used in Immunohistochemistry to show stimulation of glioma cell phagocytosis by blockage of anti-phagocytosis molecule CD47 is an ineffective process.

Fri Mar 20 00:00:00 EDT 2020

Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment.

Cell death & disease

Guillon J,Petit C,Moreau M,Toutain B,Henry C,Roché H,Bonichon-Lamichhane N,Salmon JP,Lemonnier J,Campone M,Verrièle V,Lelièvre E,Guette C,Coqueret O

14-0479 was used in Inhibition Assays to characterise the emergence of transformed tumour cells following chemotherapy-mediated senescence, and demonstrate that senescent cells favour tumour growth and metastasis, in vitro and in vivo.

Wed Feb 27 00:00:00 EST 2019

Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment.

Cell death & disease

Guillon J,Petit C,Moreau M,Toutain B,Henry C,Roché H,Bonichon-Lamichhane N,Salmon JP,Lemonnier J,Campone M,Verrièle V,Lelièvre E,Guette C,Coqueret O

Wed Feb 27 00:00:00 EST 2019

Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment.

Cell death & disease

Guillon J,Petit C,Moreau M,Toutain B,Henry C,Roché H,Bonichon-Lamichhane N,Salmon JP,Lemonnier J,Campone M,Verrièle V,Lelièvre E,Guette C,Coqueret O

Wed Feb 27 00:00:00 EST 2019

Generation and testing of clinical-grade exosomes for pancreatic cancer.

JCI insight

Mendt M,Kamerkar S,Sugimoto H,McAndrews KM,Wu CC,Gagea M,Yang S,Blanko EVR,Peng Q,Ma X,Marszalek JR,Maitra A,Yee C,Rezvani K,Shpall E,LeBleu VS,Kalluri R

14-0479 was used in Flow cytometry/Cell sorting to illustrate the process and feasibility of generating clinical-grade exosomes for various therapies of human diseases.

Thu Apr 19 00:00:00 EDT 2018

Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer.

Nature

Kamerkar S,LeBleu VS,Sugimoto H,Yang S,Ruivo CF,Melo SA,Lee JJ,Kalluri R

14-0479 was used in Flow cytometry/Cell sorting to develop a therapeutic strategy for targeting mutant KRAS in pancreatic cancer using exosomes carrying specific short interfering or short hairpin RNA.

Thu Jun 22 00:00:00 EDT 2017

CD47 promotes ovarian cancer progression by inhibiting macrophage phagocytosis.

Oncotarget

Liu R,Wei H,Gao P,Yu H,Wang K,Fu Z,Ju B,Zhao M,Dong S,Li Z,He Y,Huang Y,Yao Z

Published figure using CD47 monoclonal antibody (Product # 14-0479-82) in Flow Cytometry

Tue Jun 13 00:00:00 EDT 2017

CD47 promotes ovarian cancer progression by inhibiting macrophage phagocytosis.

Oncotarget

Liu R,Wei H,Gao P,Yu H,Wang K,Fu Z,Ju B,Zhao M,Dong S,Li Z,He Y,Huang Y,Yao Z

Published figure using CD47 monoclonal antibody (Product # 14-0479-82) in Flow Cytometry

Tue Jun 13 00:00:00 EDT 2017

SIRPα-antibody fusion proteins stimulate phagocytosis and promote elimination of acute myeloid leukemia cells.

Oncotarget

Ponce LP,Fenn NC,Moritz N,Krupka C,Kozik JH,Lauber K,Subklewe M,Hopfner KP

14-0479 was used in Flow cytometry/Cell sorting to qualify local inhibitory checkpoint monoclonal antibodies as promising approach to confine the benefit of disrupting the CD47-SIRPα axis to tumor antigen-expressing cells.

Tue Feb 14 00:00:00 EST 2017

Nanoparticle biointerfacing by platelet membrane cloaking.

Nature

Hu CM,Fang RH,Wang KC,Luk BT,Thamphiwatana S,Dehaini D,Nguyen P,Angsantikul P,Wen CH,Kroll AV,Carpenter C,Ramesh M,Qu V,Patel SH,Zhu J,Shi W,Hofman FM,Chen TC,Gao W,Zhang K,Chien S,Zhang L

14-0479 was used in Flow cytometry/Cell sorting to explore the use of the preparation of polymeric nanoparticles enclosed in the plasma membrane of human platelets.

Thu Oct 01 00:00:00 EDT 2015

CD47 is an adverse prognostic factor and a therapeutic target in gastric cancer.

Cancer medicine

Yoshida K,Tsujimoto H,Matsumura K,Kinoshita M,Takahata R,Matsumoto Y,Hiraki S,Ono S,Seki S,Yamamoto J,Hase K

14-0479 was used in Flow cytometry/Cell sorting to demonstrate that higher expressions of CD44 and CD47 in gastric cancer cells correlate with higher proliferation and tumorigenicity.

Tue Sep 01 00:00:00 EDT 2015

Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG.

Nature communications

Fischer N,Elson G,Magistrelli G,Dheilly E,Fouque N,Laurendon A,Gueneau F,Ravn U,Depoisier JF,Moine V,Raimondi S,Malinge P,Di Grazia L,Rousseau F,Poitevin Y,Calloud S,Cayatte PA,Alcoz M,Pontini G,Fagète S,Broyer L,Corbier M,Schrag D,Didelot G,Bosson N,Costes N,Cons L,Buatois V,Johnson Z,Ferlin W,Masternak K,Kosco-Vilbois M

14-0479 was used in Flow cytometry/Cell sorting to demonstrate that light chains can play a dominant role in mediating specificity and high affinity.

Thu Feb 12 00:00:00 EST 2015

Cells expressing the C/EBPbeta isoform, LIP, engulf their neighbors.

PloS one

Abreu M,Sealy L

Published figure using CD47 monoclonal antibody (Product # 14-0479-82) in Flow Cytometry

Thu Apr 11 00:00:00 EDT 2013

CD14 and toll-like receptors 2 and 4 are required for fibrillar A{beta}-stimulated microglial activation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Reed-Geaghan EG,Savage JC,Hise AG,Landreth GE

14-0479 was used in Blocking experiments to study how the engagement of the fAbeta receptor complex is linked to the induction of an activated microglial phenotype.

Wed Sep 23 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody 1D8; Antigenic surface determinant protein OA3; CD47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; Leukocyte surface antigen CD47; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47

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Gene Aliases: CD47; IAP; MER6; OA3

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UniProt ID: (Human) Q08722

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Entrez Gene ID: (Human) 961

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Function(s)

protein binding thrombospondin receptor activity cell adhesion molecule

Process(es)

cell adhesion integrin-mediated signaling pathway opsonization positive regulation of cell proliferation response to bacterium positive regulation of cell-cell adhesion extracellular matrix organization positive regulation of inflammatory response positive regulation of phagocytosis positive regulation of T cell activation leukocyte migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD47 Monoclonal Antibody (B6H12), eBioscience™

CD47 Antibody (14-0479-82) in IHC (P)

Product Details

14-0479-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Inhibition Assays (IA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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