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Invitrogen
CD49b (Integrin alpha 2) Monoclonal Antibody (HMa2), eBioscience™
FIGURE: 1 / 8
CD49b (Integrin alpha 2) Antibody (14-0491-82) in Flow
Staining of BALB/c splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.25 µg of Anti-Mouse CD49b Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111). Cells in the lymphocyte gate were used for analysis.
Product Details
14-0491-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
HMa2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_467282
Product Specific Information
Description: The HMalpha2 monoclonal antibody reacts with mouse CD49b, the 150 kDa integrin alpha2 subunit. The complex of CD49b non-covalently associated with integrin beta1 (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by some CD4^+ T cells, IEL, NK cells, platelets and epithelial cells.
Applications Reported: The HM alpha2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue (IHC-F). It has also been reported for use in in vitro functional assays (Please use Functional Grade purified HMa2, Product # 16-0491, in functional assays).
Applications Tested: The HM alpha2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; Integrin alpha-2; Platelet membrane glycoprotein Ia; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLA2 receptor
Gene Aliases: CD49B; DX5; Itga2
UniProt ID: (Mouse) Q62469
Entrez Gene ID: (Mouse) 16398
positive regulation of leukocyte migration
substrate-dependent cell migration
hypotonic response
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
positive regulation of epithelial cell migration
positive regulation of alkaline phosphatase activity
positive regulation of smooth muscle cell migration
positive regulation of cell projection organization
cell-substrate adhesion
positive regulation of collagen biosynthetic process
positive regulation of collagen binding
cell adhesion mediated by integrin
collagen-activated signaling pathway
positive regulation of DNA binding
skin morphogenesis
establishment of protein localization
positive regulation of translation
positive regulation of cell adhesion
positive regulation of smooth muscle contraction
focal adhesion assembly
positive regulation of smooth muscle cell proliferation
positive regulation of inflammatory response
positive regulation of positive chemotaxis
detection of mechanical stimulus involved in sensory perception of pain
positive regulation of transmission of nerve impulse
positive regulation of phagocytosis, engulfment
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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