Invitrogen

CD51 (Integrin alpha V) Monoclonal Antibody (RMV-7), PE, eBioscience™

8 Published Figures

27 References

View all (31) Integrin alpha V (CD51) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

CD51 (Integrin alpha V) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 9

CD51 (Integrin alpha V) Antibody (12-0512-82) in Flow

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 µg of Anti-Mouse CD51 (Integrin alpha V) PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-0512-82

Applications

Tested Dilution

Publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 25 publications 25 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RMV-7

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465704

Product Specific Information

Description: The RMV-7 monoclonal antibody reacts with the mouse CD51 molecule, the integrin alpha v chain. This approximately 120 kDa surface molecule non-covalently associates with the beta subunits of the integrin family including beta3 (CD61), beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by platelets, T cells and granulocytes and mediate adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.

Applications Reported: The RMV-7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RMV-7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8, 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands, the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Distinct skeletal stem cell types orchestrate long bone skeletogenesis.

eLife

Ambrosi TH,Sinha R,Steininger HM,Hoover MY,Murphy MP,Koepke LS,Wang Y,Lu WJ,Morri M,Neff NF,Weissman IL,Longaker MT,Chan CK

12-0512-82 was used in Flow Cytometry to address previously unappreciated shortcomings of SSC research.

Mon Jul 19 00:00:00 EDT 2021

Type V Collagen in Scar Tissue Regulates the Size of Scar after Heart Injury.

Cell

Yokota T,McCourt J,Ma F,Ren S,Li S,Kim TH,Kurmangaliyev YZ,Nasiri R,Ahadian S,Nguyen T,Tan XHM,Zhou Y,Wu R,Rodriguez A,Cohn W,Wang Y,Whitelegge J,Ryazantsev S,Khademhosseini A,Teitell MA,Chiou PY,Birk DE,Rowat AC,Crosbie RH,Pellegrini M,Seldin M,Lusis AJ,Deb A

12-0512-82 was used in Flow Cytometry to demonstrate that collagen V regulates scar size in an integrin-dependent manner.

Thu Aug 06 00:00:00 EDT 2020

Snai2 Maintains Bone Marrow Niche Cells by Repressing Osteopontin Expression.

Developmental cell

Wei Q,Nakahara F,Asada N,Zhang D,Gao X,Xu C,Alfieri A,Brodin NP,Zimmerman SE,Mar JC,Guha C,Guo W,Frenette PS

12-0512-82 was used in Flow Cytometry to conclude that SNAI2 is a critical regulator of the transcriptional network maintaining MSPCs by the suppression of osteopontin expression.

Mon Jun 08 00:00:00 EDT 2020

A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.

Nature communications

Kim JM,Yang YS,Park KH,Ge X,Xu R,Li N,Song M,Chun H,Bok S,Charles JF,Filhol-Cochet O,Boldyreff B,Dinter T,Yu PB,Kon N,Gu W,Takarada T,Greenblatt MB,Shim JH

Published figure using CD51 (Integrin alpha V) monoclonal antibody (Product # 12-0512-82) in Flow Cytometry

Fri May 08 00:00:00 EDT 2020

A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.

Nature communications

Kim JM,Yang YS,Park KH,Ge X,Xu R,Li N,Song M,Chun H,Bok S,Charles JF,Filhol-Cochet O,Boldyreff B,Dinter T,Yu PB,Kon N,Gu W,Takarada T,Greenblatt MB,Shim JH

Published figure using CD51 (Integrin alpha V) monoclonal antibody (Product # 12-0512-82) in Flow Cytometry

Fri May 08 00:00:00 EDT 2020

Nestin-GFP transgene labels skeletal progenitors in the periosteum.

Bone

Tournaire G,Stegen S,Giacomini G,Stockmans I,Moermans K,Carmeliet G,van Gastel N

12-0512-82 was used in Flow Cytometry to show that in adult mice, Nes-GFP cells are present in the periosteum and localize closely to blood vessels, but periosteal Nes-GFP cells express SSC and progenitor markers differently compared to Nes-GFP cells in the bone marrow.

Wed Apr 01 00:00:00 EDT 2020

Soluble Thy-1 reverses lung fibrosis via its integrin-binding motif.

JCI insight

Tan C,Jiang M,Wong SS,Espinoza CR,Kim C,Li X,Connors E,Hagood JS

12-0512-82 was used in Immunohistochemistry (PFA) to demonstrate that sThy-1 therapeutically inhibits the αv integrin-driven feedback loop that amplifies and sustains fibrosis.

Fri Nov 01 00:00:00 EDT 2019

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Cell reports

Balzano M,De Grandis M,Vu Manh TP,Chasson L,Bardin F,Farina A,Sergé A,Bidaut G,Charbord P,Hérault L,Bailly AL,Cartier-Michaud A,Boned A,Dalod M,Duprez E,Genever P,Coles M,Bajenoff M,Xerri L,Aurrand-Lions M,Schiff C,Mancini SJC

12-0512 was used in Flow cytometry/Cell sorting to study pro-B cells in their support of stroll cells and their interaction network allowing their retention.

Tue Mar 19 00:00:00 EDT 2019

Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.

Nature medicine

Maryanovich M,Zahalka AH,Pierce H,Pinho S,Nakahara F,Asada N,Wei Q,Wang X,Ciero P,Xu J,Leftin A,Frenette PS

Published figure using CD51 (Integrin alpha V) monoclonal antibody (Product # 12-0512-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

Published figure using CD51 (Integrin alpha V) monoclonal antibody (Product # 12-0512-82) in Flow Cytometry

Tue Dec 11 00:00:00 EST 2018

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

Published figure using CD51 (Integrin alpha V) monoclonal antibody (Product # 12-0512-82) in Flow Cytometry

Tue Dec 11 00:00:00 EST 2018

αv integrins on mesenchymal cells regulate skeletal and cardiac muscle fibrosis.

Nature communications

Murray IR,Gonzalez ZN,Baily J,Dobie R,Wallace RJ,Mackinnon AC,Smith JR,Greenhalgh SN,Thompson AI,Conroy KP,Griggs DW,Ruminski PG,Gray GA,Singh M,Campbell MA,Kendall TJ,Dai J,Li Y,Iredale JP,Simpson H,Huard J,Péault B,Henderson NC

Tue Oct 24 00:00:00 EDT 2017

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

12-0512 was used in Flow cytometry/Cell sorting to determine the contribution of lymphatic tissue to heterotopic ossification in mice.

Thu Dec 01 00:00:00 EST 2016

Regionally Restricted Hox Function in Adult Bone Marrow Multipotent Mesenchymal Stem/Stromal Cells.

Developmental cell

Rux DR,Song JY,Swinehart IT,Pineault KM,Schlientz AJ,Trulik KG,Goldstein SA,Kozloff KM,Lucas D,Wellik DM

12-0512 was used in Flow cytometry/Cell sorting to demonstrate Hox11 expression exclusively in multipotent mesenchymal stromal cells in the bone marrow of the adult zeugopod.

Mon Dec 19 00:00:00 EST 2016

Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification.

Stem cells (Dayton, Ohio)

Agarwal S,Loder SJ,Sorkin M,Li S,Shrestha S,Zhao B,Mishina Y,James AW,Levi B

12-0512-82 was used in Flow cytometry/Cell sorting to indicate that bone-cartilage-stromal progenitor cells contribute to de novo bone formation during adulthood and may hold substantial regenerative potential.

Wed Jun 01 00:00:00 EDT 2016

Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion.

Skeletal muscle

Penton CM,Badarinarayana V,Prisco J,Powers E,Pincus M,Allen RE,August PR

12-0512 was used in Flow cytometry/Cell sorting to demonstrate laminin 521 is a superior substrate for both short-term and long-term myogenic cell culture applications compared to other commonly utilized substrates.

Tue Dec 13 00:00:00 EST 2016

LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.

Nature communications

Lu XJ,Chen Q,Rong YJ,Yang GJ,Li CH,Xu NY,Yu CH,Wang HY,Zhang S,Shi YH,Chen J

12-0512 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which cytokines in the blood affect HSC homeostasis, showing that LECT2 drives HSC expansion and mobilisation.

Tue Sep 06 00:00:00 EDT 2016

Integrin-Alpha IIb Identifies Murine Lymph Node Lymphatic Endothelial Cells Responsive to RANKL.

PloS one

Cordeiro OG,Chypre M,Brouard N,Rauber S,Alloush F,Romera-Hernandez M,Bénézech C,Li Z,Eckly A,Coles MC,Rot A,Yagita H,Léon C,Ludewig B,Cupedo T,Lanza F,Mueller CG

12-0512 was used in Flow cytometry/Cell sorting to demonstrate that stromal reticular cells activate lymphatic endothelial cells via RANKL and support the action of hematopoietic cell-derived lymphotoxin.

Thu Aug 11 00:00:00 EDT 2016

BMP signaling mediated by constitutively active Activin type 1 receptor (ACVR1) results in ectopic bone formation localized to distal extremity joints.

Developmental biology

Agarwal S,Loder SJ,Brownley C,Eboda O,Peterson JR,Hayano S,Wu B,Zhao B,Kaartinen V,Wong VC,Mishina Y,Levi B

12-0512 was used in Flow cytometry/Cell sorting to characterise a novel mouse model of heterotopic ossification.

Wed Apr 15 00:00:00 EDT 2015

Megakaryocytes regulate hematopoietic stem cell quiescence through CXCL4 secretion.

Nature medicine

Bruns I,Lucas D,Pinho S,Ahmed J,Lambert MP,Kunisaki Y,Scheiermann C,Schiff L,Poncz M,Bergman A,Frenette PS

12-0512 was used in Flow cytometry/Cell sorting to indicate that a terminally differentiated cell type derived from HSCs contributes to the HSC niche, directly regulating HSC behavior.

Sat Nov 01 00:00:00 EDT 2014

The plexin C1 receptor promotes acute inflammation.

European journal of immunology

König K,Marth L,Roissant J,Granja T,Jennewein C,Devanathan V,Schneider M,Köhler D,Zarbock A,Rosenberger P

12-0512 was used in Flow cytometry/Cell sorting to study the role of plexin C1 during the acute inflammatory response.

Mon Sep 01 00:00:00 EDT 2014

Chemotherapy-induced bone marrow nerve injury impairs hematopoietic regeneration.

Nature medicine

Lucas D,Scheiermann C,Chow A,Kunisaki Y,Bruns I,Barrick C,Tessarollo L,Frenette PS

12-0512 was used in Flow cytometry/Cell sorting to demonstrate the potential benefit of adrenergic nerve protection for shielding hematopoietic niches from injury.

Sat Jun 01 00:00:00 EDT 2013

Chemotherapy-induced bone marrow nerve injury impairs hematopoietic regeneration.

Nature medicine

Lucas D,Scheiermann C,Chow A,Kunisaki Y,Bruns I,Barrick C,Tessarollo L,Frenette PS

12-0512 was used in Flow cytometry/Cell sorting to demonstrate the potential benefit of adrenergic nerve protection for shielding hematopoietic niches from injury.

Sat Jun 01 00:00:00 EDT 2013

Molecular ultrasound imaging using a targeted contrast agent for assessing early tumor response to antiangiogenic therapy.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Sorace AG,Saini R,Mahoney M,Hoyt K

Published figure using CD51 (Integrin alpha V) monoclonal antibody (Product # 12-0512-82) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2012

Role for E-cadherin as an inhibitory receptor on epidermal gammadelta T cells.

Journal of immunology (Baltimore, Md. : 1950)

Uchida Y,Kawai K,Ibusuki A,Kanekura T

12-0512 was used in Flow cytometry/Cell sorting to analyse functional differences between E-cadherin and α(E)β(7) integrin expressed on murine dendritic epidermal T cells.

Wed Jun 15 00:00:00 EDT 2011

A triple-targeted ultrasound contrast agent provides improved localization to tumor vasculature.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Warram JM,Sorace AG,Saini R,Umphrey HR,Zinn KR,Hoyt K

Published figure using CD51 (Integrin alpha V) monoclonal antibody (Product # 12-0512-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2011

Galectin-9 suppresses tumor metastasis by blocking adhesion to endothelium and extracellular matrices.

Glycobiology

Nobumoto A,Nagahara K,Oomizu S,Katoh S,Nishi N,Takeshita K,Niki T,Tominaga A,Yamauchi A,Hirashima M

12-0512 was used in Flow cytometry/Cell sorting to investigate the efficiency and mechanism by which galectin-9 inhibits lung metastasis in experimental mouse models.

Mon Sep 01 00:00:00 EDT 2008

Characterization of fibroblasts responsible for cartilage destruction in arthritis.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Treese C,Mittag A,Lange F,Tarnok A,Loesche A,Emmrich F,Lehmann J,Sack U

12-0512 was used in Flow cytometry/Cell sorting to show that LS48 is an excellent tool to gain a deeper understanding of synovial fibroblasts in rheumatoid arthritis.

Tue Apr 01 00:00:00 EDT 2008

Tumor growth impedes natural-killer-cell maturation in the bone marrow.

Blood

Richards JO,Chang X,Blaser BW,Caligiuri MA,Zheng P,Liu Y

12-0512 was used in Flow cytometry/Cell sorting to demonstrate that tumour growth can impede functional maturation of NK cells, most likely by interrupting the requisite IL-15 signalling pathway.

Sat Jul 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: alpha v integrin; CD51; GP3A; Integrin alpha-V; ITGB3; vitronectin receptor alpha polypeptide (VNRA); Vitronectin receptor subunit alpha; VNR

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Gene Aliases: 1110004F14Rik; 2610028E01Rik; CD51; D430040G12Rik; Itgav

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UniProt ID: (Mouse) P43406

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Entrez Gene ID: (Mouse) 16410

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Function(s)

opsonin binding fibronectin binding protease binding protein kinase C binding receptor binding voltage-gated calcium channel activity insulin-like growth factor I binding peptide binding metal ion binding protein heterodimerization activity extracellular matrix binding extracellular matrix protein binding integrin

Process(es)

angiogenesis blood vessel development cellular calcium ion homeostasis inflammatory response cell adhesion cell-matrix adhesion positive regulation of cytosolic calcium ion concentration integrin-mediated signaling pathway multicellular organism development positive regulation of cell proliferation fertilization negative regulation of macrophage derived foam cell differentiation negative regulation of lipid storage cell growth cell migration cell differentiation positive regulation of cell migration cell-substrate adhesion negative regulation of lipid transport detection of molecule of bacterial origin positive regulation of osteoblast proliferation heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading regulation of ion transmembrane transport gonad morphogenesis endodermal cell differentiation apolipoprotein A-I-mediated signaling pathway T cell activation apoptotic cell clearance positive regulation of MAPK cascade endothelial cell migration regulation of bone resorption negative regulation of low-density lipoprotein particle receptor biosynthetic process positive regulation of cell adhesion viral entry into host cell focal adhesion assembly negative regulation of lipoprotein metabolic process regulation of phagocytosis negative chemotaxis release of sequestered calcium ion into cytosol trophoblast giant cell differentiation ERK1 and ERK2 cascade calcium ion transmembrane transport response to nitric oxide extrinsic apoptotic signaling pathway in absence of ligand regulation of apoptotic cell clearance negative regulation of entry of bacterium into host cell negative regulation of extrinsic apoptotic signaling pathway

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CD51 (Integrin alpha V) Monoclonal Antibody (RMV-7), PE, eBioscience™

CD51 (Integrin alpha V) Antibody (12-0512-82) in Flow

Product Details

12-0512-82

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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