Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (HA58), Functional Grade, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

16 Published Figures

20 References

View all (173) ICAM-1 (CD54) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 18

CD54 (ICAM-1) Antibody (16-0549-82) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD54 (ICAM-1) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

CD54 (ICAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD54 (ICAM-1) Antibody in Immunohistochemistry (IHC)

CD54 (ICAM-1) Antibody in Western Blot (WB)

CD54 (ICAM-1) Antibody in Flow Cytometry, Neutralization (Flow, Neu)

CD54 (ICAM-1) Antibody in ChIP Assay (ChIP)

CD54 (ICAM-1) Antibody in Functional Assay (Functional)

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Product Details

16-0549-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 10 publications 10 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HA58

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468981

Product Specific Information

Description: The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response.

Applications Reported: The HA58 antibody has been reported for use in flow cytometric analysis. HA58 has also been reported in blocking of in vitro mixed lymphocyte reaction.

Applications Tested: The HA58 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosis.

Food science & nutrition

Festa J,Hussain A,Hackney A,Desai U,Sahota TS,Singh H,Da Boit M

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Impact of latency-reversing agents on human macrophage physiology.

Immunity, inflammation and disease

Hany L,Turmel MO,Barat C,Ouellet M,Tremblay MJ

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2023

Multimodal CRISPR perturbations of GWAS loci associated with coronary artery disease in vascular endothelial cells.

PLoS genetics

Wünnemann F,Fotsing Tadjo T,Beaudoin M,Lalonde S,Lo KS,Kleinstiver BP,Lettre G

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Anti-Inflammatory Activity of Ferula assafoetida Oleo-Gum-Resin (Asafoetida) against TNF-α-Stimulated Human Umbilical Vein Endothelial Cells (HUVECs).

Mediators of inflammation

Mobasheri L,Khorashadizadeh M,Safarpour H,Mohammadi M,Anani Sarab G,Askari VR

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Flow Cytometry

Tue Sep 13 00:00:00 EDT 2022

Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2.

PLoS pathogens

Shrestha P,Davis DA,Jaeger HK,Stream A,Aisabor AI,Yarchoan R

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2.

PLoS pathogens

Shrestha P,Davis DA,Jaeger HK,Stream A,Aisabor AI,Yarchoan R

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Preservation of microvascular barrier function requires CD31 receptor-induced metabolic reprogramming.

Nature communications

Cheung KCP,Fanti S,Mauro C,Wang G,Nair AS,Fu H,Angeletti S,Spoto S,Fogolari M,Romano F,Aksentijevic D,Liu W,Li B,Cheng L,Jiang L,Vuononvirta J,Poobalasingam TR,Smith DM,Ciccozzi M,Solito E,Marelli-Berg FM

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Functional assay

Fri Jul 17 00:00:00 EDT 2020

Preservation of microvascular barrier function requires CD31 receptor-induced metabolic reprogramming.

Nature communications

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Functional assay

Fri Jul 17 00:00:00 EDT 2020

EWS-FLI1 low Ewing sarcoma cells demonstrate decreased susceptibility to T-cell-mediated tumor cell apoptosis.

Oncotarget

Bailey KM,Julian CM,Klinghoffer AN,Bernard H,Lucas PC,McAllister-Lucas LM

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Flow Cytometry

Tue May 21 00:00:00 EDT 2019

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in ChIP assay

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in ChIP assay

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in ChIP assay

Thu Jun 01 00:00:00 EDT 2017

CD54-NOTCH1 axis controls tumor initiation and cancer stem cell functions in human prostate cancer.

Theranostics

Li C,Liu S,Yan R,Han N,Wong KK,Li L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Immunohistochemistry

Wed Oct 18 00:00:00 EDT 2017

CD54-NOTCH1 axis controls tumor initiation and cancer stem cell functions in human prostate cancer.

Theranostics

Li C,Liu S,Yan R,Han N,Wong KK,Li L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Immunohistochemistry

Wed Oct 18 00:00:00 EDT 2017

CD54-NOTCH1 axis controls tumor initiation and cancer stem cell functions in human prostate cancer.

Theranostics

Li C,Liu S,Yan R,Han N,Wong KK,Li L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Immunohistochemistry

Wed Oct 18 00:00:00 EDT 2017

Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.

PloS one

An N,Andrukhov O,Tang Y,Falkensammer F,Bantleon HP,Ouyang X,Rausch-Fan X

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2015

Quantitative modeling assesses the contribution of bond strengthening, rebinding and force sharing to the avidity of biomolecule interactions.

PloS one

Lo Schiavo V,Robert P,Limozin L,Bongrand P

16-0549 was used in Immuno-assay to study the strength of ligand and cell-surface receptor interactions in a model system.

Fri Feb 15 00:00:00 EST 2013

Neuroprotective effects of mesenchymal stem cells derived from human embryonic stem cells in transient focal cerebral ischemia in rats.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Liu YP,Seçkin H,Izci Y,Du ZW,Yan YP,Başkaya MK

16-0549 was used in Flow cytometry/Cell sorting to show intravenously administered embryonic mesenchymal stem cells differentiated into neuronal and endothelial cells, and were neuroprotective in transient focal cerebral ischemia.

Wed Apr 01 00:00:00 EDT 2009

Mycobacterium tuberculosis-induced gamma interferon production by natural killer cells requires cross talk with antigen-presenting cells involving Toll-like receptors 2 and 4 and the mannose receptor in tuberculous pleurisy.

Infection and immunity

Schierloh P,Yokobori N,Alemán M,Landoni V,Geffner L,Musella RM,Castagnino J,Baldini M,Abbate E,de la Barrera SS,Sasiain MC

16-0549 was used in Blocking experiments to study how mycobacterium tuberculosis-induced gamma interferon production by natural killer cells requires cross talk with antigen-presenting cells involving Toll-like receptors 2 and 4 and the mannose receptor in tuberculous pleurisy.

Thu Nov 01 00:00:00 EDT 2007

The human natural killer cell immune synapse.

Proceedings of the National Academy of Sciences of the United States of America

Davis DM,Chiu I,Fassett M,Cohen GB,Mandelboim O,Strominger JL

Tue Dec 21 00:00:00 EST 1999

Bioinformatics

Protein Aliases: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells

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Gene Aliases: BB2; CD54; ICAM1; P3.58

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UniProt ID: (Human) P05362

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Entrez Gene ID: (Human) 3383

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Function(s)

virus receptor activity receptor activity transmembrane signaling receptor activity integrin binding protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD54 (ICAM-1) Monoclonal Antibody (HA58), Functional Grade, eBioscience™

CD54 (ICAM-1) Antibody (16-0549-82) in Flow

Product Details

16-0549-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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