Invitrogen

CD62P (P-Selectin) Monoclonal Antibody (Psel.KO2.3), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

20 Published Figures

22 References

View all (58) P-Selectin (CD62P) antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

CD62P (P-Selectin) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 22

CD62P (P-Selectin) Antibody (12-0626-82) in Flow

Staining of Thrombin-activated human platelets with Anti-Human CD41a FITC (Product # 11-0419-42) and 0.125 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or 0.125 µg of Anti-Human/Mouse CD62P (P-Selectin) PE (right). Total viable cells were used for analysis.

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Product Details

12-0626-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Psel.KO2.3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210863

Product Specific Information

Description: The Psel.KO2.3 monoclonal antibody reacts with mouse CD62P (also known as P-selectin and GMP-140), a 140-kDa cell adhesion molecule that can be induced on endothelial cells and platelets. During inflammation, this selectin mediates adhesion of monocytes and neutrophils to activated platelets, as well as leukocyte rolling on endothelial cells. The major ligand for CD62P on neutrophils is P-selectin glycoprotein ligand-1 (PSGL-1). This monoclonal antibody recognizes an epitope that is conserved across several species (e.g. mouse, human, and rat) and has not been reported to block cell adhesion.

Applications Reported: This Psel.KO2.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Psel.KO2.3 antibody was tested by flow cytometric analysis on thrombin-activated human platelets. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Myristica fragrans Extract Inhibits Platelet Desialylation and Activation to Ameliorate Sepsis-Associated Thrombocytopenia in a Murine CLP-Induced Sepsis Model.

International journal of molecular sciences

Jeong SH,Park JY,Ryu YB,Kim WS,Lee IC,Kim JH,Kim D,Ha JH,Lee BW,Nam J,Cho KO,Kwon HJ

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 12-0626-82) in Flow Cytometry

Tue May 16 00:00:00 EDT 2023

Small-Molecule Cyclophilin Inhibitors Potently Reduce Platelet Procoagulant Activity.

International journal of molecular sciences

Van Bael J,Vandenbulcke A,Ahmed-Belkacem A,Guichou JF,Pawlotsky JM,Samyn J,Barendrecht AD,Maas C,De Meyer SF,Vanhoorelbeke K,Tersteeg C

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 12-0626-82) in Flow Cytometry

Wed Apr 12 00:00:00 EDT 2023

Whole Blood Procoagulant Platelet Flow Cytometry Protocol for Heparin-Induced Thrombocytopenia (HIT) and Vaccine-Induced Immune Thrombotic Thrombocytopenia (VITT) Testing.

Methods in molecular biology (Clifton, N.J.)

Lee CSM,Powell MC,Chen VM

12-0626-82 was used in Flow cytometry/Cell sorting to present a protocol for a novel whole blood flow cytometry-based assay to detect procoagulant platelets in healthy donor blood in response to plasma from patients suspected of HIT or VITT.

Mon May 22 00:00:00 EDT 2023

Cyclical depressurization degranulates platelets in an agonist-free mechanism of platelet activation.

PloS one

Velasquez-Mao AJ,Velasquez M,Vandsburger MH

12-0626-82 was used in Flow cytometry/Cell sorting to investigate the mechanobiology of platelet degranulation under hydrostatic depressurization, revealing a unique case where CD62P and PAC1 do not interchangeably indicate platelet activation.

Mon Sep 19 00:00:00 EDT 2022

Involvement of P2Y₁₂ receptors in a nitroglycerin-induced model of migraine in male mice.

British journal of pharmacology

Gölöncsér F,Baranyi M,Iring A,Hricisák L,Otrokocsi L,Benyó Z,Sperlágh B

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 12-0626-82) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

The role of Sphingomyelin synthase 2 (SMS2) in platelet activation and its clinical significance.

Thrombosis journal

Guo Y,Chang L,Zhang G,Gao Z,Lin H,Zhang Y,Hu L,Chen S,Fan B,Zhang S,Xue R

12-0626-82 was used in Flow Cytometry to indicate that SMS2 acts as a positive regulator of platelet activation and thrombosis, and provides a theoretical basis for the potential use of D609 in anti-thrombosis treatment.

Wed Apr 28 00:00:00 EDT 2021

Activation of Platelet NLRP3 Inflammasome in Crohn's Disease.

Frontiers in pharmacology

Zhang G,Chen H,Guo Y,Zhang W,Jiang Q,Zhang S,Han L,Chen S,Xue R

12-0626-82 was used in Flow cytometry/Cell sorting to indicate that the ROS-NLRP3 inflammasome-interleukin-1β axis may contribute to platelet hyperactivity in active CD.

Fri Jul 16 00:00:00 EDT 2021

Mesenchymal stem cells attenuate liver fibrosis by targeting Ly6C^hi/lo macrophages through activating the cytokine-paracrine and apoptotic pathways.

Cell death discovery

Li YH,Shen S,Shao T,Jin MT,Fan DD,Lin AF,Xiang LX,Shao JZ

12-0626-82 was used in Flow Cytometry to provide insights into the functional role of bone marrow-derived sesenchymal stem cell in alleviating liver fibrosis.

Mon Sep 13 00:00:00 EDT 2021

Longitudinal RNA-Seq Analysis of the Repeatability of Gene Expression and Splicing in Human Platelets Identifies a Platelet SELP Splice QTL.

Circulation research

Rondina MT,Voora D,Simon LM,Schwertz H,Harper JF,Lee O,Bhatlekar SC,Li Q,Eustes AS,Montenont E,Campbell RA,Tolley ND,Kosaka Y,Weyrich AS,Bray PF,Rowley JW

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 12-0626-82) in Flow Cytometry

Fri Feb 14 00:00:00 EST 2020

Loss of Tet2 affects platelet function but not coagulation in mice.

Blood science (Baltimore, Md.)

Wang B,Xia M,Chen T,Li M,Shi D,Wang X,Pang A,Zhou J,Yuan W,Chu Y

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 12-0626-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Expression and Clinical Prognostic Value of Platelet NLRP3 in Acute Coronary Syndrome.

International journal of general medicine

Peng H,Wu H,Zhang G,Zhang W,Guo Y,Chang L,Chen S,Xue R,Zhang S

12-0626 was used in Flow cytometry/Cell sorting to indicate that platelet NLRP3 was a novel potential prognostic factor for patients with acute coronary syndrome that underwent percutaneous coronary intervention.

Sun Apr 17 00:00:00 EDT 2022

Expression and Clinical Prognostic Value of Platelet NLRP3 in Acute Coronary Syndrome.

International journal of general medicine

Peng H,Wu H,Zhang G,Zhang W,Guo Y,Chang L,Chen S,Xue R,Zhang S

Sun Apr 17 00:00:00 EDT 2022

Targeted anti-IL-1β platelet microparticles for cardiac detoxing and repair.

Science advances

Li Z,Hu S,Huang K,Su T,Cores J,Cheng K

12-0626-82 was used in Flow cytometry/Cell sorting to address the issue of conventional IL-1β blockers lacking targeting specificity resulting in increased risk of serious side effects.

Sat Feb 01 00:00:00 EST 2020

Mesenchymal stromal cells lower platelet activation and assist in platelet formation in vitro.

JCI insight

Mendelson A,Strat AN,Bao W,Rosston P,Fallon G,Ohrn S,Zhong H,Lobo C,An X,Yazdanbakhsh K

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 12-0626-82) in Flow Cytometry

Thu Aug 22 00:00:00 EDT 2019

Circulating apoptotic bodies maintain mesenchymal stem cell homeostasis and ameliorate osteopenia via transferring multiple cellular factors.

Cell research

Liu D,Kou X,Chen C,Liu S,Liu Y,Yu W,Yu T,Yang R,Wang R,Zhou Y,Shi S

12-0626 was used in Flow cytometry/Cell sorting to identify a previously unknown role of apoptotic bodies in maintaining MSC and bone homeostasis in both physiological and pathological contexts and implies the potential use of apoptotic bodies to treat osteoporosis.

Sat Sep 01 00:00:00 EDT 2018

Circulating apoptotic bodies maintain mesenchymal stem cell homeostasis and ameliorate osteopenia via transferring multiple cellular factors.

Cell research

Liu D,Kou X,Chen C,Liu S,Liu Y,Yu W,Yu T,Yang R,Wang R,Zhou Y,Shi S

Sat Sep 01 00:00:00 EDT 2018

Novel antibodies against GPIbα inhibit pulmonary metastasis by affecting vWF-GPIbα interaction.

Journal of hematology & oncology

Qi Y,Chen W,Liang X,Xu K,Gu X,Wu F,Fan X,Ren S,Liu J,Zhang J,Li R,Liu J,Liang X

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 12-0626-82) in Flow Cytometry

Mon Sep 17 00:00:00 EDT 2018

Neutrophil-derived S100 calcium-binding proteins A8/A9 promote reticulated thrombocytosis and atherogenesis in diabetes.

The Journal of clinical investigation

Kraakman MJ,Lee MK,Al-Sharea A,Dragoljevic D,Barrett TJ,Montenont E,Basu D,Heywood S,Kammoun HL,Flynn M,Whillas A,Hanssen NM,Febbraio MA,Westein E,Fisher EA,Chin-Dusting J,Cooper ME,Berger JS,Goldberg IJ,Nagareddy PR,Murphy AJ

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 12-0626-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 12-0626-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 12-0626-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Refrigerated platelets stored in whole blood up to 5 days adhere to thrombi formed during hemorrhagic hypotension in rats.

Journal of thrombosis and haemostasis : JTH

Torres Filho IP,Torres LN,Valdez C,Salgado C,Cap AP,Dubick MA

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 12-0626-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Platelet-derived HMGB1 is a critical mediator of thrombosis.

The Journal of clinical investigation

Vogel S,Bodenstein R,Chen Q,Feil S,Feil R,Rheinlaender J,Schäffer TE,Bohn E,Frick JS,Borst O,Münzer P,Walker B,Markel J,Csanyi G,Pagano PJ,Loughran P,Jessup ME,Watkins SC,Bullock GC,Sperry JL,Zuckerbraun BS,Billiar TR,Lotze MT,Gawaz M,Neal MD

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 12-0626-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Sphingosine-1-phosphate receptor 3 promotes leukocyte rolling by mobilizing endothelial P-selectin.

Nature communications

Nussbaum C,Bannenberg S,Keul P,Gräler MH,Gonçalves-de-Albuquerque CF,Korhonen H,von Wnuck Lipinski K,Heusch G,de Castro Faria Neto HC,Rohwedder I,Göthert JR,Prasad VP,Haufe G,Lange-Sperandio B,Offermanns S,Sperandio M,Levkau B

12-0626 was used in Flow cytometry/Cell sorting to identify S1P as a direct and indirect contributor to leukocyte rolling and characterise the receptors mediating its action.

Thu Apr 02 00:00:00 EDT 2015

Sphingosine-1-phosphate receptor 3 promotes leukocyte rolling by mobilizing endothelial P-selectin.

Nature communications

12-0626 was used in Flow cytometry/Cell sorting to identify S1P as a direct and indirect contributor to leukocyte rolling and characterise the receptors mediating its action.

Thu Apr 02 00:00:00 EDT 2015

Maresin 1 mitigates LPS-induced acute lung injury in mice.

British journal of pharmacology

Gong J,Wu ZY,Qi H,Chen L,Li HB,Li B,Yao CY,Wang YX,Wu J,Yuan SY,Yao SL,Shang Y

12-0626 was used in Flow cytometry/Cell sorting to demonstrate that high-dose MaR1 mitigates LPS-induced lung injury in mice by inhibiting neutrophil adhesions and decreasing pro-inflammatory cytokines levels.

Tue Jul 01 00:00:00 EDT 2014

Cathepsin G-dependent modulation of platelet thrombus formation in vivo by blood neutrophils.

PloS one

Faraday N,Schunke K,Saleem S,Fu J,Wang B,Zhang J,Morrell C,Dore S

12-0626 was used in Flow cytometry/Cell sorting to demonstrate the potential of neutrophil cathepsin G as a target for novel anti-thrombotic therapies.

Mon Apr 14 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD62 antigen-like family member P; CD62P; FLJ45155; GMP-140; Granule membrane protein 140; granule membrane protein 140kDa; granulocyte membrane protein; LECAM3; Leukocyte-endothelial cell adhesion molecule 3; P-selectin; PADGEM; Platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; RP1-86F14.2; selectin P (granule membrane protein 140kDa, antigen CD62)

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Gene Aliases: CD62; CD62P; GMP-140; GMP140; GMRP; GRMP; LECAM3; PADGEM; PSEL; SELP

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UniProt ID: (Human) P16109, (Mouse) Q01102

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Entrez Gene ID: (Human) 6403, (Mouse) 20344

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Function(s)

lipopolysaccharide binding glycoprotein binding protein binding heparin binding sialic acid binding fucose binding glycosphingolipid binding calcium-dependent protein binding oligosaccharide binding carbohydrate binding

Process(es)

platelet degranulation positive regulation of leukocyte migration regulation of cellular extravasation inflammatory response cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion positive regulation of platelet activation positive regulation of phosphatidylinositol 3-kinase signaling response to organic cyclic compound response to lipopolysaccharide regulation of integrin activation calcium-mediated signaling using intracellular calcium source positive regulation of cell adhesion defense response to Gram-negative bacterium leukocyte migration leukocyte tethering or rolling single organismal cell-cell adhesion cellular response to interleukin-6

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CD62P (P-Selectin) Monoclonal Antibody (Psel.KO2.3), PE, eBioscience™

CD62P (P-Selectin) Antibody (12-0626-82) in Flow

Product Details

12-0626-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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