Invitrogen

CD64 Monoclonal Antibody (10.1), eBioscience™

FIGURE: 1 / 6

CD64 Antibody (14-0649-82) in Flow

Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human CD64 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the monocyte gate were used for analysis.

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Product Details

14-0649-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Flow Cytometry (Flow)

1 µg/test

View 7 publications 7 publications

Neutralization (Neu)

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

In vitro Assay (IV)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

10.1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467322

Product Specific Information

Description: The 10.1 monoclonal antibody reacts with human CD64 (FcRI), a 75 kDa type I transmembrane protein. CD64 is the high affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and regulation of cytokine production. Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative.

Applications Reported: The 10.1 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested: The 10.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation.

Cancers

Prat M,Salon M,Allain T,Dubreuil O,Noël G,Preisser L,Jean B,Cassard L,Lemée F,Tabah-Fish I,Pipy B,Jeannin P,Prost JF,Barret JM,Coste A

Published figure using CD64 monoclonal antibody (Product # 14-0649-82) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus.

Nature communications

Doni A,Parente R,Laface I,Magrini E,Cunha C,Colombo FS,Lacerda JF,Campos A,Mapelli SN,Petroni F,Porte R,Schorn T,Inforzato A,Mercier T,Lagrou K,Maertens J,Lambris JD,Bottazzi B,Garlanda C,Botto M,Carvalho A,Mantovani A

14-0649-82 was used in Flow Cytometry to conclude that SAP is a fluid phase pattern recognition molecule essential for resistance against A. fumigatus.

Fri Jun 18 00:00:00 EDT 2021

Extracorporeal Hemadsorption versus Glucocorticoids during Cardiopulmonary Bypass: A Prospective, Randomized, Controlled Trial.

Cardiovascular therapeutics

Taleska Stupica G,Sostaric M,Bozhinovska M,Rupert L,Bosnic Z,Jerin A,Ihan A,Klokocovnik T,Podbregar M

Published figure using CD64 monoclonal antibody (Product # 14-0649-82) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Patient iPSC-Derived Macrophages to Study Inborn Errors of the IFN-γ Responsive Pathway.

Cells

Haake K,Neehus AL,Buchegger T,Kühnel MP,Blank P,Philipp F,Oleaga-Quintas C,Schulz A,Grimley M,Goethe R,Jonigk D,Kalinke U,Boisson-Dupuis S,Casanova JL,Bustamante J,Lachmann N

Published figure using CD64 monoclonal antibody (Product # 14-0649-82) in Flow Cytometry

Wed Feb 19 00:00:00 EST 2020

Recombinant H22(scFv) blocks CD64 and prevents the capture of anti-TNF monoclonal antibody. A potential strategy to enhance anti-TNF therapy.

mAbs

Hristodorov D,Mladenov R,Brehm H,Fischer R,Barth S,Thepen T

Published figure using CD64 (Fc gamma Receptor 1) monoclonal antibody (Product # 14-0649-82) in Flow Cytometry

Mon Aug 24 00:00:00 EDT 2015

Distinct Fcγ receptors mediate the effect of serum amyloid p on neutrophil adhesion and fibrocyte differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Cox N,Pilling D,Gomer RH

14-0649 was used in Flow cytometry/Cell sorting to suggest that SAP binds to FcγRI on monocytes to inhibit fibrocyte differentiation, and binds to FcγRIIa on neutrophils to reduce neutrophil adhesion.

Fri Aug 15 00:00:00 EDT 2014

Cytokine-induced immune complex binding to the high-affinity IgG receptor, FcγRI, in the presence of monomeric IgG.

Blood

van der Poel CE,Karssemeijer RA,Boross P,van der Linden JA,Blokland M,van de Winkel JG,Leusen JH

14-0649 was used in Blocking experiments to contribute to understanding of how FcγRI can participate in the clearance of opsonized particles despite saturation by monomeric IgG.

Thu Dec 09 00:00:00 EST 2010

Histidine-rich glycoprotein is a novel plasma pattern recognition molecule that recruits IgG to facilitate necrotic cell clearance via FcgammaRI on phagocytes.

Blood

Poon IK,Hulett MD,Parish CR

14-0649 was used in in vitro experiments to show that histidine-rich glycoprotein can complex with IgG, facilitating a pro-inflammatory innate immune response, promoting the clearance of necrotic cells.

Thu Mar 25 00:00:00 EDT 2010

Internalization and coreceptor expression are critical for TLR2-mediated recognition of lipoteichoic acid in human peripheral blood.

Journal of immunology (Baltimore, Md. : 1950)

Bunk S,Sigel S,Metzdorf D,Sharif O,Triantafilou K,Triantafilou M,Hartung T,Knapp S,von Aulock S

14-0649 was used in Blocking experiments to demonstrate that Dlgt-LTA-induced immune activation critically depends on LTA immobilization and the presence of human serum components.

Wed Sep 15 00:00:00 EDT 2010

Ligand of scavenger receptor class A indirectly induces maturation of human blood dendritic cells via production of tumor necrosis factor-alpha.

Blood

Jin JO,Park HY,Xu Q,Park JI,Zvyagintseva T,Stonik VA,Kwak JY

14-0649 was used in Flow cytometry/Cell sorting to demonstrate a potential pathway for SR-A to enhance T-cell stimulatory capacity.

Thu Jun 04 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD64; CD64 antigen; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc gamma receptor; Fc gamma receptor Ia; Fc-gamma receptor 1; Fc-gamma receptor I A1; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FcgRI; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; RP11-196G18.2

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Gene Aliases: CD64; CD64A; FCG1; FCGR1; FCGR1A; FCRI; IGFR1

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UniProt ID: (Human) P12314

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Entrez Gene ID: (Human) 2209

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Function(s)

receptor signaling protein activity protein binding IgG binding immunoglobulin receptor superfamily

Process(es)

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent receptor-mediated endocytosis phagocytosis, engulfment immune response signal transduction Fc-gamma receptor signaling pathway involved in phagocytosis regulation of immune response interferon-gamma-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD64 Monoclonal Antibody (10.1), eBioscience™

CD64 Antibody (14-0649-82) in Flow

Product Details

14-0649-82

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Neutralization (Neu)

Inhibition Assays (IA)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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