Invitrogen

CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), FITC, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

24 Published Figures

30 References

View all (177) CD68 antibodies

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Protocols

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Datasheet

Protocols

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CD68 Antibody (11-0689-42) in Flow

Knockout of CD68 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR904279_LV) and LentiArray Cas9 Lentivirus (Product # A32064). For Flow cytometry analysis, THP-1 CD68 Knock out cells were stained intracellularly using the intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.125 µg Mouse IgG2b kappa Isotype Control (eBMG2b), FITC, eBioscience™ (Product # 11-4732-81, yellow histogram) or 0.125 µg CD68 Monoclonal Antibody (eBioY1/82A (Y... View More

CD68 Antibody in Immunocytochemistry (ICC/IF)

CD68 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

CD68 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD68 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD68 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD68 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of CD68 using Anti-CD68,FITC Mouse Monoclonal Antibody (eBioY1/82A (Y1/82A)) (Product # 11-0689-41) shows expression of proteins only in THP-1 cell line upon differentiation into Macrophage (M0 phase). {TM}

Product Details

11-0689-42

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

0.125 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 17 publications 17 publications

Immunomicroscopy (IM)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioY1/82A (Y1/82A)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11149303

Product Specific Information

Description: The eBioY1/82 antibody reacts with CD68 which belongs to the sialomucin family and is closely related to the family of lysosomal-associated membrane proteins (lamps) and scavenger receptor. CD68 is predominantly an intracellular protein, found mainly in the late endosomal compartment but can be detected in smaller amounts on the surface of cells of mainly myeloid derived cells; monocytes and macrophages, dendritic cells (and in some langerhan cells), neutrophils, basophils mast cells, myeloid progenitor cells, and a subset of CD34+ hemopoietic bone marrow progenitor cells. The function has not been fully elucidated but based on it's homolgy and structure suggests a role in antigen processing or presentation and protection of the lysosomal membrane from hydrolytic enzymes. Reports have also seen expression in activated T cells, and about 40% of peripheral blood B-lymphocytes and 50% of all B-ALL.

Staining with eBioY1/82A shows surface staining on B cells and a minor percentage of monocytes while intracellular staining results in about 50% of B cells and most monocytes.

Applications Reported: This eBioY1/82A (Y1/82A) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioY1/82A (Y1/82A) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Activation of the JNK/COX-2/HIF-1α axis promotes M1 macrophage via glycolytic shift in HIV-1 infection.

Life science alliance

Zhang J,Yuan Z,Li X,Wang F,Wei X,Kang Y,Mo C,Jiang J,Liang H,Ye L

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2023

Targeting Glioblastoma-Associated Macrophages for Photodynamic Therapy Using AGuIX^®-Design Nanoparticles.

Pharmaceutics

Lerouge L,Gries M,Chateau A,Daouk J,Lux F,Rocchi P,Cedervall J,Olsson AK,Tillement O,Frochot C,Acherar S,Thomas N,Barberi-Heyob M

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Immunohistochemistry

Mon Mar 20 00:00:00 EDT 2023

Complete Freund's Adjuvant Induces a Fibroblast-like Synoviocytes (FLS) Metabolic and Migratory Phenotype in Resident Fibroblasts of the Inoculated Footpad at the Earliest Stage of Adjuvant-Induced Arthritis.

Cells

González-Chávez SA,Chaparro-Barrera E,Alvarado-Jáquez MF,Cuevas-Martínez R,Ochoa-Albíztegui RE,Pacheco-Tena C

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Immunohistochemistry

Wed Mar 08 00:00:00 EST 2023

LINC00240/miR-155 axis regulates function of trophoblasts and M2 macrophage polarization via modulating oxidative stress-induced pyroptosis in preeclampsia.

Molecular medicine (Cambridge, Mass.)

Wu HY,Liu K,Zhang JL

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Flow Cytometry

Sat Sep 24 00:00:00 EDT 2022

PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.

CNS neuroscience & therapeutics

Zhang H,Wang Y,Zhao Y,Liu T,Wang Z,Zhang N,Dai Z,Wu W,Cao H,Feng S,Zhang L,Cheng Q,Liu Z

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

NIL10: A New IL10-Receptor Binding Nanoparticle That Induces Cardiac Protection in Mice and Pigs Subjected to Acute Myocardial Infarction through STAT3/NF-κB Activation.

Pharmaceutics

Tesoro L,Hernández I,Ramírez-Carracedo R,Díez-Mata J,Alcharani N,Jiménez-Guirado B,Ovejero-Paredes K,Filice M,Zamorano JL,Saura M,Zaragoza C,Botana L

11-0689-42 was used in Immunohistochemistry, Flow Cytometry, Immunocytochemistry to show that NIL10, a novel anti-inflammatory nanoparticle, induces cardiac protection after acute myocardial infarction following cardiac ischemia/reperfusion.

Mon Sep 26 00:00:00 EDT 2022

Different Induction of PD-L1 (CD274) and PD-1 (CD279) Expression in THP-1-Differentiated Types 1 and 2 Macrophages.

Journal of inflammation research

Lai CY,Tseng PC,Chen CL,Satria RD,Wang YT,Lin CF

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Flow Cytometry

Wed Apr 27 00:00:00 EDT 2022

Decreased ferroportin in hepatocytes promotes macrophages polarize towards an M2-like phenotype and liver fibrosis.

Scientific reports

Cai C,Zeng D,Gao Q,Ma L,Zeng B,Zhou Y,Wang H

11-0689-42 was used in Western Blot to demonstrate if downregulated ferroportin expression in hepatocytes can promote the proliferation and polarization of macrophages, leading to hepatic fibrosis.

Mon Jun 28 00:00:00 EDT 2021

Decreased ferroportin in hepatocytes promotes macrophages polarize towards an M2-like phenotype and liver fibrosis.

Scientific reports

Cai C,Zeng D,Gao Q,Ma L,Zeng B,Zhou Y,Wang H

11-0689-42 was used in Western Blot to demonstrate if downregulated ferroportin expression in hepatocytes can promote the proliferation and polarization of macrophages, leading to hepatic fibrosis.

Mon Jun 28 00:00:00 EDT 2021

Decreased ferroportin in hepatocytes promotes macrophages polarize towards an M2-like phenotype and liver fibrosis.

Scientific reports

Cai C,Zeng D,Gao Q,Ma L,Zeng B,Zhou Y,Wang H

11-0689-42 was used in Western Blot to demonstrate if downregulated ferroportin expression in hepatocytes can promote the proliferation and polarization of macrophages, leading to hepatic fibrosis.

Mon Jun 28 00:00:00 EDT 2021

Decreased ferroportin in hepatocytes promotes macrophages polarize towards an M2-like phenotype and liver fibrosis.

Scientific reports

Cai C,Zeng D,Gao Q,Ma L,Zeng B,Zhou Y,Wang H

11-0689-42 was used in Western Blot to demonstrate if downregulated ferroportin expression in hepatocytes can promote the proliferation and polarization of macrophages, leading to hepatic fibrosis.

Mon Jun 28 00:00:00 EDT 2021

Decreased ferroportin in hepatocytes promotes macrophages polarize towards an M2-like phenotype and liver fibrosis.

Scientific reports

Cai C,Zeng D,Gao Q,Ma L,Zeng B,Zhou Y,Wang H

11-0689-42 was used in Western Blot to demonstrate if downregulated ferroportin expression in hepatocytes can promote the proliferation and polarization of macrophages, leading to hepatic fibrosis.

Mon Jun 28 00:00:00 EDT 2021

The role of CD68+ macrophage in classical Hodgkin lymphoma patients from Egypt.

Diagnostic pathology

Mohamed O,El Bastawisy A,Allahlobi N,Abdellateif MS,Zekri ARN,Shaarawy S,Korany Z,Mohanad M,Bahnassy AA

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Flow Cytometry

Tue Feb 04 00:00:00 EST 2020

The role of CD68+ macrophage in classical Hodgkin lymphoma patients from Egypt.

Diagnostic pathology

Mohamed O,El Bastawisy A,Allahlobi N,Abdellateif MS,Zekri ARN,Shaarawy S,Korany Z,Mohanad M,Bahnassy AA

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Flow Cytometry

Tue Feb 04 00:00:00 EST 2020

Nanotopographical Coatings Induce an Early Phenotype-Specific Response of Primary Material-Resident M1 and M2 Macrophages.

Materials (Basel, Switzerland)

Schmitz T,Jannasch M,Weigel T,Moseke C,Gbureck U,Groll J,Walles H,Hansmann J

11-0689-42 was used in Flow Cytometry to investigate the influence of tubular TiO2 and fractal TiN nanostructures on primary human macrophages with M1 or M2-phenotype.

Wed Mar 04 00:00:00 EST 2020

Nanotopographical Coatings Induce an Early Phenotype-Specific Response of Primary Material-Resident M1 and M2 Macrophages.

Materials (Basel, Switzerland)

Schmitz T,Jannasch M,Weigel T,Moseke C,Gbureck U,Groll J,Walles H,Hansmann J

11-0689-42 was used in Flow Cytometry to investigate the influence of tubular TiO2 and fractal TiN nanostructures on primary human macrophages with M1 or M2-phenotype.

Wed Mar 04 00:00:00 EST 2020

Tumor-associated macrophages promote human hepatoma Huh-7 cell migration and invasion through the Gli2/IGF-II/ERK1/2 axis by secreting TGF-β1.

Cancer biology & therapy

Liu M,Zhong YB,Shao J,Zhang C,Shi C

11-0689-42 was used in Flow Cytometry, Immunocytochemistry to conclude that the TGF-β1 secreted by TAMs promotes the migration, invasion and EMT of human hepatoma Huh-7 cells through the Gli2/IGF-II/ERK1/2 pathway.

Sun Nov 01 00:00:00 EDT 2020

Role of adipokine zinc-α₂-glycoprotein in coronary heart disease.

American journal of physiology. Endocrinology and metabolism

Huang D,Mao X,Peng J,Cheng M,Bai T,Du M,Huang K,Liu B,Yang L,Huang K,Zhang F

11-0689 was used in Flow cytometry/Cell sorting to investigate the role of AZGP1 in atherosclerosis and CHD.

Sun Dec 01 00:00:00 EST 2019

Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis.

Science advances

Izadi D,Layton TB,Williams L,McCann F,Cabrita M,Espirito Santo AI,Xie W,Fritzsche M,Colin-York H,Feldmann M,Midwood KS,Nanchahal J

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

Baicalein suppress EMT of breast cancer by mediating tumor-associated macrophages polarization.

American journal of cancer research

Zhao X,Qu J,Liu X,Wang J,Ma X,Zhao X,Yang Q,Yan W,Zhao Z,Hui Y,Bai H,Zhang S

11-0689 was used in Flow cytometry/Cell sorting to explore the potential effect of baicalein on macrophage polarisation and epithelial-mesenchymal transition of breast cancer.

Wed Sep 30 00:00:00 EDT 2020

Macrophagic CD146 promotes foam cell formation and retention during atherosclerosis.

Cell research

Luo Y,Duan H,Qian Y,Feng L,Wu Z,Wang F,Feng J,Yang D,Qin Z,Yan X

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Immunofluorescence

Wed Mar 01 00:00:00 EST 2017

In vitro chemotaxis and tissue remodeling assays quantitatively characterize foreign body reaction.

ALTEX

Jannasch M,Weigel T,Engelhardt L,Wiezoreck J,Gaetzner S,Walles H,Schmitz T,Hansmann J

11-0689 was used in Flow cytometry/Cell sorting to investigate the material dependency of fibroblast chemotaxis and tissue remodelling mediated by material-resident macrophages.

Tue Oct 10 00:00:00 EDT 2017

Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.

Journal of neurovirology

Garcia-Mesa Y,Jay TR,Checkley MA,Luttge B,Dobrowolski C,Valadkhan S,Landreth GE,Karn J,Alvarez-Carbonell D

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

A comparative multi-parametric in vitro model identifies the power of test conditions to predict the fibrotic tendency of a biomaterial.

Scientific reports

Jannasch M,Gaetzner S,Weigel T,Walles H,Schmitz T,Hansmann J

11-0689 was used in Flow cytometry/Cell sorting to show that biomaterial ranking obtained under native plasma test conditions showed a high predictive accuracy compared to in vivo assessments.

Wed May 10 00:00:00 EDT 2017

Suppression of the inflammatory response by disease-inducible interleukin-10 gene therapy in a three-dimensional micromass model of the human synovial membrane.

Arthritis research & therapy

Broeren MG,de Vries M,Bennink MB,Arntz OJ,van Lent PL,van der Kraan PM,van den Berg WB,van den Hoogen FH,Koenders MI,van de Loo FA

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Immunohistochemistry

Fri Aug 12 00:00:00 EDT 2016

MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.

Bioscience reports

Zhong Y,Yi C

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.

Scientific reports

Tsai CS,Lin YW,Huang CY,Shih CM,Tsai YT,Tsao NW,Lin CS,Shih CC,Jeng H,Lin FY

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Immunocytochemistry

Fri Dec 02 00:00:00 EST 2016

Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.

Scientific reports

Tsai CS,Lin YW,Huang CY,Shih CM,Tsai YT,Tsao NW,Lin CS,Shih CC,Jeng H,Lin FY

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Immunocytochemistry

Fri Dec 02 00:00:00 EST 2016

Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.

Scientific reports

Tsai CS,Lin YW,Huang CY,Shih CM,Tsai YT,Tsao NW,Lin CS,Shih CC,Jeng H,Lin FY

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Immunocytochemistry

Fri Dec 02 00:00:00 EST 2016

Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.

Scientific reports

Tsai CS,Lin YW,Huang CY,Shih CM,Tsai YT,Tsao NW,Lin CS,Shih CC,Jeng H,Lin FY

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Immunocytochemistry

Fri Dec 02 00:00:00 EST 2016

Expression of Lectin-Like Transcript 1, the Ligand for CD161, in Rheumatoid Arthritis.

PloS one

Chalan P,Bijzet J,Huitema MG,Kroesen BJ,Brouwer E,Boots AM

11-0689 was used in Flow cytometry/Cell sorting to investigate the presence and identity of LLT1-expressing cells in RA synovial fluid (SF) and synovial tissue (ST).

Mon Apr 11 00:00:00 EDT 2016

Expression of Lectin-Like Transcript 1, the Ligand for CD161, in Rheumatoid Arthritis.

PloS one

Chalan P,Bijzet J,Huitema MG,Kroesen BJ,Brouwer E,Boots AM

11-0689 was used in Flow cytometry/Cell sorting to investigate the presence and identity of LLT1-expressing cells in RA synovial fluid (SF) and synovial tissue (ST).

Mon Apr 11 00:00:00 EDT 2016

Doxycycline inhibits polarization of macrophages to the proangiogenic M2-type and subsequent neovascularization.

The Journal of biological chemistry

He L,Marneros AG

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Flow Cytometry

Fri Mar 21 00:00:00 EDT 2014

Doxycycline inhibits polarization of macrophages to the proangiogenic M2-type and subsequent neovascularization.

The Journal of biological chemistry

He L,Marneros AG

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Flow Cytometry

Fri Mar 21 00:00:00 EDT 2014

Hepatocellular proliferation correlates with inflammatory cell and cytokine changes in a murine model of nonalchoholic fatty liver disease.

PloS one

Vansaun MN,Mendonsa AM,Lee Gorden D

Published figure using CD68 monoclonal antibody (Product # 11-0689-42) in Immunohistochemistry

Sun Jun 15 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD68; CD68 antigen; Gp110; macrophage antigen CD68; Macrosialin; scavenger receptor class D, member 1

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Gene Aliases: CD68; GP110; LAMP4; SCARD1

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UniProt ID: (Human) P34810

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Entrez Gene ID: (Human) 968

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Function(s)

membrane trafficking regulatory protein

Process(es)

cellular response to organic substance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), FITC, eBioscience™

CD68 Antibody (11-0689-42) in Flow

Product Details

11-0689-42

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunomicroscopy (IM)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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