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Invitrogen
CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), Alexa Fluor™ 700, eBioscience™
FIGURE: 1 / 11
CD71 (Transferrin Receptor) Antibody (56-0711-82) in Flow
C57BL/6 mouse splenocytes were stained with CD45R Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 700 (Product # 56-4321-80) (left) or 0.125 µg of CD71 Monoclonal Antibody, Alexa Fluor 700 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
56-0711-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
R17217 (RI7 217.1.4)
Conjugate
Excitation/Emission Max
696/719 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784765
Product Specific Information
Description: The R17217 monoclonal antibody reacts with mouse CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71. Expression level differences have been observed in different mouse strains.
Applications Reported: This R17217 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This R17217 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser
Target Information
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD antigen CD71; CD71; mammary tumor virus receptor 1; TR; Transferrin receptor protein 1
Gene Aliases: 2610028K12Rik; AI195355; AI426448; AU015758; CD71; E430033M20Rik; Mtvr-1; Mtvr1; p90; TFR; TFR1; Tfrc; TR; Trfr
UniProt ID: (Mouse) Q62351
Entrez Gene ID: (Mouse) 22042
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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