Invitrogen

CD80 (B7-1) Monoclonal Antibody (2D10.4), PE, eBioscience™

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Antibody Testing Data (1)
Published Figures (17)

CD80 (B7-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 18

CD80 (B7-1) Antibody (12-0809-42) in Flow

Staining of the Daudi cell line with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD80 (B7-1) PE (purple histogram). Total viable cells were used for analysis.

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Product Details

12-0809-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 26 publications 26 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2D10.4

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1311209

Product Specific Information

Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Applications Reported: The 2D10.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 2D10.4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Engineered Human Dendritic Cell Exosomes as Effective Delivery System for Immune Modulation.

International journal of molecular sciences

Elsayed R,Elashiry M,Tran C,Yang T,Carroll A,Liu Y,Hamrick M,Cutler CW

Published figure using CD80 (B7-1) monoclonal antibody (Product # 12-0809-42) in Flow Cytometry

Tue Jul 11 00:00:00 EDT 2023

Interleukin 6 exacerbates the progression of warm autoimmune hemolytic anemia by influencing the activity and function of B cells.

Scientific reports

Zhao M,Chen L,Yang J,Zhang Z,Wang H,Shao Z,Liu X,Xing L

12-0809-42 was used in Flow cytometry/Cell sorting to explore the effect of IL-6 on the activity and secretory function of B cells and analyze its effect on clinical indicators and efficacy in wAIHA patients.

Mon Aug 14 00:00:00 EDT 2023

B cell-intrinsic changes with age do not impact antibody-secreting cell formation but delay B cell participation in the germinal centre reaction.

Aging cell

Lee JL,Fra-Bido SC,Burton AR,Innocentin S,Hill DL,Linterman MA

Published figure using CD80 (B7-1) monoclonal antibody (Product # 12-0809-42) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2022

Macrophage Membrane-Camouflaged shRNA and Doxorubicin: A pH-Dependent Release System for Melanoma Chemo-Immunotherapy.

Research (Washington, D.C.)

Yang C,Ming Y,Zhou K,Hao Y,Hu D,Chu B,He X,Yang Y,Qian Z

Published figure using CD80 (B7-1) monoclonal antibody (Product # 12-0809-42) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Sestrin2 Is Increased in Calcific Aortic Disease and Inhibits Osteoblastic Differentiation in Valvular Interstitial Cells via the Nuclear Factor E2-related Factor 2 Pathway.

Journal of cardiovascular pharmacology

Wang H,Xi J,Zhang Z,Li J,Guo L,Li N,Sun Y,Li X,Han X

12-0809-42 was used in Flow cytometry/Cell sorting to suggest that Sesn2 is increased in CAVD aortic valves and may participate in the development of CAVD by regulating oxidative stress via the Nrf2 pathway.

Sat Oct 01 00:00:00 EDT 2022

Response of human macrophages to gamma radiation is mediated via expression of endogenous retroviruses.

PLoS pathogens

Mikhalkevich N,O'Carroll IP,Tkavc R,Lund K,Sukumar G,Dalgard CL,Johnson KR,Li W,Wang T,Nath A,Iordanskiy S

Published figure using CD80 (B7-1) monoclonal antibody (Product # 12-0809-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Different Induction of PD-L1 (CD274) and PD-1 (CD279) Expression in THP-1-Differentiated Types 1 and 2 Macrophages.

Journal of inflammation research

Lai CY,Tseng PC,Chen CL,Satria RD,Wang YT,Lin CF

Published figure using CD80 (B7-1) monoclonal antibody (Product # 12-0809-42) in Flow Cytometry

Wed Apr 27 00:00:00 EDT 2022

Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells.

Reproductive biology and endocrinology : RB&E

Li L,Liao Z,Ye M,Jiang J

12-0809-42 was used in Flow cytometry/Cell sorting to study recombinant human IL-37 (rhIL-37) is an inhibitor of inflammation.

Tue Aug 24 00:00:00 EDT 2021

Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells.

Reproductive biology and endocrinology : RB&E

Li L,Liao Z,Ye M,Jiang J

12-0809-42 was used in Flow cytometry/Cell sorting to study recombinant human IL-37 (rhIL-37) is an inhibitor of inflammation.

Tue Aug 24 00:00:00 EDT 2021

Engineering of a trispecific tumor-targeted immunotherapy incorporating 4-1BB co-stimulation and PD-L1 blockade.

Oncoimmunology

Warmuth S,Gunde T,Snell D,Brock M,Weinert C,Simonin A,Hess C,Tietz J,Johansson M,Spiga FM,Heiz R,Flückiger N,Wagen S,Zeberer J,Diem D,Mahler D,Wickihalder B,Muntwiler S,Chatterjee B,Küttner B,Bommer B,Yaman Y,Lichtlen P,Urech D

12-0809-42 was used in Flow cytometry/Cell sorting to engineer a tri-specific antibody-based molecule that stimulates intratumoral 4-1BB and blocks PD-L1/PD-1 signaling without systemic toxicity and with clinically favorable pharmacokinetics.

Tue Jul 19 00:00:00 EDT 2022

Astragalus polysaccharides (PG2) Enhances the M1 Polarization of Macrophages, Functional Maturation of Dendritic Cells, and T Cell-Mediated Anticancer Immune Responses in Patients with Lung Cancer.

Nutrients

Bamodu OA,Kuo KT,Wang CH,Huang WC,Wu ATH,Tsai JT,Lee KY,Yeh CT,Wang LS

Published figure using CD80 (B7-1) monoclonal antibody (Product # 12-0809-42) in Flow Cytometry

Fri Sep 20 00:00:00 EDT 2019

Mesenchymal Stromal Cells and Their Extracellular Vesicles Enhance the Anti-Inflammatory Phenotype of Regulatory Macrophages by Downregulating the Production of Interleukin (IL)-23 and IL-22.

Frontiers in immunology

Hyvärinen K,Holopainen M,Skirdenko V,Ruhanen H,Lehenkari P,Korhonen M,Käkelä R,Laitinen S,Kerkelä E

12-0809 was used in Flow cytometry/Cell sorting to investigate the effects of both human bone marrow-derived mesenchymal stromal cells and MSC-derived extracellular vesicles on mature human regulatory macrophages.

Fri Jun 07 00:00:00 EDT 2019

Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70.

Oncology letters

Zhang F,Zhu Y,Fan G,Hu S

Published figure using CD80 (B7-1) monoclonal antibody (Product # 12-0809-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.

Oncology letters

Xiu W,Ma J,Lei T,Zhang M

12-0809 was used in Flow cytometry/Cell sorting to study the ability of AG490 to partly reverse the inhibitory effect of bladder cancer cells on dendritic cells, and induce their anti-tumour immunity response.

Sat Sep 01 00:00:00 EDT 2018

Role of Nuclear Factor (Erythroid-Derived 2)-Like 2 Signaling for Effects of Fumaric Acid Esters on Dendritic Cells.

Frontiers in immunology

Hammer A,Waschbisch A,Knippertz I,Zinser E,Berg J,Jörg S,Kuhbandner K,David C,Pi J,Bayas A,Lee DH,Haghikia A,Gold R,Steinkasserer A,Linker RA

Wed Nov 20 00:00:00 EST 2019

Allostimulatory capacity of conditionally immortalized proximal tubule cell lines for bioartificial kidney application.

Scientific reports

Mihajlovic M,van den Heuvel LP,Hoenderop JG,Jansen J,Wilmer MJ,Westheim AJF,Allebes WA,Stamatialis D,Hilbrands LB,Masereeuw R

12-0809 was used in Flow cytometry/Cell sorting to assess the immunosafety of conditionally immortalized proximal tubule cell lines for use in bioartificial kidneys, specifically by investigating their allogeneic efects.

Wed Aug 02 00:00:00 EDT 2017

Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization.

Journal of immunology (Baltimore, Md. : 1950)

Joo H,Upchurch K,Zhang W,Ni L,Li D,Xue Y,Li XH,Hori T,Zurawski S,Liu YJ,Zurawski G,Oh S

12-0809 was used in Flow cytometry/Cell sorting to investigate the role of Dectin-1 in dendritic cells, showing opposing roles between plasmacytoid and myeloid dendritic cells in Th2 polarisation.

Sat Aug 15 00:00:00 EDT 2015

Cervical (pre)neoplastic microenvironment promotes the emergence of tolerogenic dendritic cells via RANKL secretion.

Oncoimmunology

Demoulin SA,Somja J,Duray A,Guénin S,Roncarati P,Delvenne PO,Herfs MF,Hubert PM

12-0809 was used in Flow cytometry/Cell sorting to investigate how the emergence of tolerogenic dendritic cells via RANKL secretion is promoted by cervical (pre)neoplastic microenvironment.

Mon Jun 01 00:00:00 EDT 2015

Omental implantation of BOECs in hemophilia dogs results in circulating FVIII antigen and a complex immune response.

Blood

Ozelo MC,Vidal B,Brown C,Notley C,Hegadorn C,Webster S,Harpell L,Ahlin J,Winterborn A,Handforth J,Arruda VR,Hough C,Lillicrap D

12-0809 was used in Flow cytometry/Cell sorting to investigate the immune response to treatment with FVIII-expressing autologous BOECs.

Thu Jun 26 00:00:00 EDT 2014

M-CSF inhibits anti-HIV-1 activity of IL-32, but they enhance M2-like phenotypes of macrophages.

Journal of immunology (Baltimore, Md. : 1950)

Osman A,Bhuyan F,Hashimoto M,Nasser H,Maekawa T,Suzu S

12-0809 was used in Flow cytometry/Cell sorting to investigate the functional interplay between M-CSF and IL-32 acting on macrophages.

Sun Jun 01 00:00:00 EDT 2014

Brugia malayi microfilariae induce a regulatory monocyte/macrophage phenotype that suppresses innate and adaptive immune responses.

PLoS neglected tropical diseases

O'Regan NL,Steinfelder S,Venugopal G,Rao GB,Lucius R,Srikantam A,Hartmann S

12-0809 was used in Flow cytometry/Cell sorting to show that monocytes stimulated with Brugia malayi microfilarial lysate develop a defined regulatory phenotype.

Wed Oct 01 00:00:00 EDT 2014

Leptin up-regulates TLR2 in human monocytes.

Journal of leukocyte biology

Jaedicke KM,Roythorne A,Padget K,Todryk S,Preshaw PM,Taylor JJ

12-0809 was used in Flow cytometry/Cell sorting to elucidate the effect of leptin on PRRs.

Mon Apr 01 00:00:00 EDT 2013

Novel role of plasmacytoid dendritic cells in humans: induction of interleukin-10-producing Treg cells by plasmacytoid dendritic cells in patients with rheumatoid arthritis responding to therapy.

Arthritis and rheumatism

Kavousanaki M,Makrigiannakis A,Boumpas D,Verginis P

12-0809 was used in Flow cytometry/Cell sorting to explore the contribution of dendritic cells to the development of regulatory T cells in a human autoimmune disease setting.

Fri Jan 01 00:00:00 EST 2010

CCL11 blocks IL-4 and GM-CSF signaling in hematopoietic cells and hinders dendritic cell differentiation via suppressor of cytokine signaling expression.

Journal of leukocyte biology

Stevenson NJ,Addley MR,Ryan EJ,Boyd CR,Carroll HP,Paunovic V,Bursill CA,Miller HC,Channon KM,McClurg AE,Armstrong MA,Coulter WA,Greaves DR,Johnston JA

12-0809 was used in Flow cytometry/Cell sorting to study the blockade effects of cytokine signaling by CCL11, which resulted in reduced differentiation and endocytic ability of dendritic cells, implicating CCL11-induced SOCS as mediators of chemotactic inflammatory control.

Sun Feb 01 00:00:00 EST 2009

Immunological aspects of REIC/Dkk-3 in monocyte differentiation and tumor regression.

International journal of oncology

Watanabe M,Kashiwakura Y,Huang P,Ochiai K,Futami J,Li SA,Takaoka M,Nasu Y,Sakaguchi M,Huh NH,Kumon H

12-0809-42 was used in Flow cytometry/Cell sorting to study that recombinant REIC/Dkk-3 protein induced the differentiation of human CD14+ monocytes into a novel cell type.

Sun Mar 01 00:00:00 EST 2009

Enhanced efficacy of CTLA-4 fusion anti-caries DNA vaccines in gnotobiotic hamsters.

Acta pharmacologica Sinica

Zhang F,Li YH,Fan MW,Jia R,Xu QA,Guo JH,Yu F,Tian QW

12-0809 was used in Flow cytometry/Cell sorting to prove CTLLA-4-antigen fusion enhances anti-caries DNA vaccines.

Wed Aug 01 00:00:00 EDT 2007

Renal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates.

The Journal of clinical investigation

Bashuda H,Kimikawa M,Seino K,Kato Y,Ono F,Shimizu A,Yagita H,Teraoka S,Okumura K

Fri Jul 01 00:00:00 EDT 2005

Clinical significance of costimulatory molecules CD80/CD86 expression in IgA nephropathy.

Kidney international

Wu Q,Jinde K,Endoh M,Sakai H

12-0809 was used in Flow cytometry/Cell sorting to suggest that CD80 and CD86 activate T cells in IgAN, CD80/CD86 expressions correlated with renal function at the time of renal biopsy, and monocyte/macrophages and tubular epithelial cells act as APC.

Mon Mar 01 00:00:00 EST 2004

Bioinformatics

Protein Aliases: Activation B7-1 antigen; B-lymphocyte activation antigen B7; B7; BB1; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; sCD 80; sCD80; soluble CD 80; soluble CD80; T-lymphocyte activation antigen CD80; TS/A-1

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Gene Aliases: B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; CD80; LAB7

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UniProt ID: (Human) P33681

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Entrez Gene ID: (Human) 941

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Function(s)

virus receptor activity protein binding coreceptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity immunoglobulin receptor superfamily

Process(es)

cell-cell signaling positive regulation of signal transduction regulation of phosphatidylinositol 3-kinase signaling T cell costimulation intracellular signal transduction T cell activation positive regulation of interleukin-2 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process positive regulation of T-helper 1 cell differentiation positive regulation of transcription, DNA-templated positive regulation of alpha-beta T cell proliferation viral entry into host cell phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of peptidyl-tyrosine phosphorylation cellular response to lipopolysaccharide

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CD80 (B7-1) Monoclonal Antibody (2D10.4), PE, eBioscience™

CD80 (B7-1) Antibody (12-0809-42) in Flow

Product Details

12-0809-42

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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