Invitrogen

CD86 (B7-2) Monoclonal Antibody (GL1), APC-eFluor™ 780, eBioscience™

CD86 (B7-2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 99

CD86 (B7-2) Antibody (47-0862-82) in Flow

C57BL/6 mouse splenocytes were stimulated for 72 hours with LPS (Product # 00-4976-03) and then stained with CD19 Monoclonal Antibody, PE (Product # 12-0193-82) and 0.06 µg of Rat IgG2a kappa Isotype Control, APC-eFluor 780 (Product # 47-4321-82) (left) or 0.06 µg of CD86 Monoclonal Antibody, APC-eFluor 780 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

CD86 (B7-2) Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

47-0862-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 96 publications 96 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

GL1

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815162

Product Specific Information

Description: The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.

Applications Reported: This GL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This GL1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Target Information

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

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Cell reports

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Nature communications

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Journal of neuroinflammation

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Stem cell research & therapy

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Frontiers in immunology

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Frontiers in immunology

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International journal of oncology

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Molecular medicine reports

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MiR-103 protects from recurrent spontaneous abortion via inhibiting STAT1 mediated M1 macrophage polarization.

International journal of biological sciences

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Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

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Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Restructures the Immune Contexture to Improve Checkpoint Blockade Efficacy.

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Wed Dec 16 00:00:00 EST 2020

Lactobacillus reuteri 5454 and Bifidobacterium animalis ssp. lactis 5764 improve colitis while differentially impacting dendritic cells maturation and antimicrobial responses.

Scientific reports

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Tue Mar 24 00:00:00 EDT 2020

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2020

Polyanhydride Nanoparticles Induce Low Inflammatory Dendritic Cell Activation Resulting in CD8⁺ T Cell Memory and Delayed Tumor Progression.

International journal of nanomedicine

Darling R,Senapati S,Christiansen J,Liu L,Ramer-Tait AE,Narasimhan B,Wannemuehler M

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

Epigenetically modulated FOXM1 suppresses dendritic cell maturation in pancreatic cancer and colon cancer.

Molecular oncology

Zhou Z,Chen H,Xie R,Wang H,Li S,Xu Q,Xu N,Cheng Q,Qian Y,Huang R,Shao Z,Xiang M

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells.

Stem cells and development

Fujii S,Yoshida S,Inagaki E,Hatou S,Tsubota K,Takahashi M,Shimmura S,Sugita S

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

CD1d highly expressed on DCs reduces lung tumor burden by enhancing antitumor immunity.

Oncology reports

Li Y,Zhao C,Liu J,Lu Z,Lu M,Gu J,Liu R

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

cGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation Following Mycobacterium bovis Infection.

International journal of molecular sciences

Li Q,Liu C,Yue R,El-Ashram S,Wang J,He X,Zhao D,Zhou X,Xu L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Tue Feb 19 00:00:00 EST 2019

Arp2/3 complex-driven spatial patterning of the BCR enhances immune synapse formation, BCR signaling and B cell activation.

eLife

Bolger-Munro M,Choi K,Scurll JM,Abraham L,Chappell RS,Sheen D,Dang-Lawson M,Wu X,Priatel JJ,Coombs D,Hammer JA,Gold MR

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Mon Jun 03 00:00:00 EDT 2019

Mutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction.

Frontiers in immunology

Fenninger F,Han J,Stanwood SR,Nohara LL,Arora H,Choi KB,Munro L,Pfeifer CG,Shanina I,Horwitz MS,Jefferies WA

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Thu Oct 29 00:00:00 EDT 2020

Efficient oral vaccination by bioengineering virus-like particles with protozoan surface proteins.

Nature communications

Serradell MC,Rupil LL,Martino RA,Prucca CG,Carranza PG,Saura A,Fernández EA,Gargantini PR,Tenaglia AH,Petiti JP,Tonelli RR,Reinoso-Vizcaino N,Echenique J,Berod L,Piaggio E,Bellier B,Sparwasser T,Klatzmann D,Luján HD

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Mon Jan 21 00:00:00 EST 2019

Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.

Journal of nanobiotechnology

Yuan SJ,Xu YH,Wang C,An HC,Xu HZ,Li K,Komatsu N,Zhao L,Chen X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Thu Oct 17 00:00:00 EDT 2019

M1-Polarized Macrophages Promote Self-Renewing Phenotype of Hepatic Progenitor Cells with Jagged1-Notch Signalling Involved: Relevance in Primary Sclerosing Cholangitis.

Journal of immunology research

Li H,Sun S,Lei Q,Lei P,Cai X,Wan C,Shen G

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Mon Apr 08 00:00:00 EDT 2019

Ginsenoside Rg3 Mitigates Atherosclerosis Progression in Diabetic apoE-/- Mice by Skewing Macrophages to the M2 Phenotype.

Frontiers in pharmacology

Guo M,Xiao J,Sheng X,Zhang X,Tie Y,Wang L,Zhao L,Ji X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Targeting macrophage and microglia activation with colony stimulating factor 1 receptor inhibitor is an effective strategy to treat injury-triggered neuropathic pain.

Molecular pain

Lee S,Shi XQ,Fan A,West B,Zhang J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Mon Oct 22 00:00:00 EDT 2018

T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells.

Nature communications

Kim HR,Mun Y,Lee KS,Park YJ,Park JS,Park JH,Jeon BN,Kim CH,Jun Y,Hyun YM,Kim M,Lee SM,Park CS,Im SH,Jun CD

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2018

Antibody Tumor Targeting Is Enhanced by CD27 Agonists through Myeloid Recruitment.

Cancer cell

Turaj AH,Hussain K,Cox KL,Rose-Zerilli MJJ,Testa J,Dahal LN,Chan HTC,James S,Field VL,Carter MJ,Kim HJ,West JJ,Thomas LJ,He LZ,Keler T,Johnson PWM,Al-Shamkhani A,Thirdborough SM,Beers SA,Cragg MS,Glennie MJ,Lim SH

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Mon Dec 11 00:00:00 EST 2017

FcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells.

Frontiers in immunology

Pan YG,Yu YL,Lin CC,Lanier LL,Chu CL

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

IRF6 Regulates Alternative Activation by Suppressing PPARγ in Male Murine Macrophages.

Endocrinology

Li C,Ying W,Huang Z,Brehm T,Morin A,Vella AT,Zhou B

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Discrimination of the heterogeneity of bone marrow‑derived dendritic cells.

Molecular medicine reports

Wang J,Dai X,Hsu C,Ming C,He Y,Zhang J,Wei L,Zhou P,Wang CY,Yang J,Gong N

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Calreticulin is an effective immunologic adjuvant to tumor-associated antigens.

Experimental and therapeutic medicine

Wang J,Gao ZP,Qin S,Liu CB,Zou LL

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Batf3-dependent CD8α⁺ Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.

EBioMedicine

Li Y,Liu X,Duan W,Tian H,Zhu G,He H,Yao S,Yi S,Song W,Tang H

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Paeoniflorin Ameliorates Experimental Autoimmune Encephalomyelitis via Inhibition of Dendritic Cell Function and Th17 Cell Differentiation.

Scientific reports

Zhang H,Qi Y,Yuan Y,Cai L,Xu H,Zhang L,Su B,Nie H

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Mon Feb 06 00:00:00 EST 2017

Tumor-derived exosomes educate dendritic cells to promote tumor metastasis via HSP72/HSP105-TLR2/TLR4 pathway.

Oncoimmunology

Shen Y,Guo D,Weng L,Wang S,Ma Z,Yang Y,Wang P,Wang J,Cai Z

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Repulsive guidance molecule a blockade exerts the immunoregulatory function in DCs stimulated with ABP and LPS.

Human vaccines & immunotherapeutics

Xu X,Gao Y,Zhai Z,Zhang S,Shan F,Feng J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Tue Aug 02 00:00:00 EDT 2016

Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NFκB Pathway Suppression.

Journal of diabetes research

Zhou Z,Tang Y,Jin X,Chen C,Lu Y,Liu L,Shen C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Tue Jun 06 00:00:00 EDT 2017

CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice.

Experimental and therapeutic medicine

Li JG,DU YM,Yan ZD,Yan J,Zhuansun YX,Chen R,Zhang W,Feng SL,Ran PX

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Temporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury.

Frontiers in cellular neuroscience

Hellström Erkenstam N,Smith PL,Fleiss B,Nair S,Svedin P,Wang W,Boström M,Gressens P,Hagberg H,Brown KL,Sävman K,Mallard C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Sat Jan 29 00:00:00 EST 2022

Irradiation of necrotic cancer cells, employed for pulsing dendritic cells (DCs), potentiates DC vaccine-induced antitumor immunity against high-grade glioma.

Oncoimmunology

Vandenberk L,Garg AD,Verschuere T,Koks C,Belmans J,Beullens M,Agostinis P,De Vleeschouwer S,Van Gool SW

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

D-Alanylation of Teichoic Acids and Loss of Poly-N-Acetyl Glucosamine in Staphylococcus aureus during Exponential Growth Phase Enhance IL-12 Production in Murine Dendritic Cells.

PloS one

Lund LD,Ingmer H,Frøkiær H

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Thu Jul 14 00:00:00 EDT 2016

Injectable, spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy.

Nature biotechnology

Kim J,Li WA,Choi Y,Lewin SA,Verbeke CS,Dranoff G,Mooney DJ

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Reducing progression of experimental lupus nephritis via inhibition of the B7/CD28 signaling pathway.

Molecular medicine reports

Huang L,Kong Y,Wang J,Sun J,Shi Q,Qiu YH

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Injectable cryogel-based whole-cell cancer vaccines.

Nature communications

Bencherif SA,Warren Sands R,Ali OA,Li WA,Lewin SA,Braschler TM,Shih TY,Verbeke CS,Bhatta D,Dranoff G,Mooney DJ

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Wed Aug 12 00:00:00 EDT 2015

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Lung dendritic cells undergo maturation and polarization towards a T helper type 2-stimulating phenotype in a mouse model of asthma: Role of nerve growth factor.

Experimental and therapeutic medicine

Qin Q,Wang Z,Pan P,Cao Z,Xia Q,Tan H,Hu C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Sat Nov 01 00:00:00 EDT 2014

Alkylglycerols modulate the proliferation and differentiation of non-specific agonist and specific antigen-stimulated splenic lymphocytes.

PloS one

Qian L,Zhang M,Wu S,Zhong Y,Van Tol E,Cai W

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Fri Jul 31 00:00:00 EDT 2015

A new animal model of spontaneous autoimmune peripheral polyneuropathy: implications for Guillain-Barré syndrome.

Acta neuropathologica communications

Yang M,Rainone A,Shi XQ,Fournier S,Zhang J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Wed Jan 08 00:00:00 EST 2014

Neutralization of TSLP inhibits airway remodeling in a murine model of allergic asthma induced by chronic exposure to house dust mite.

PloS one

Chen ZG,Zhang TT,Li HT,Chen FH,Zou XL,Ji JZ,Chen H

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Tue Aug 20 00:00:00 EDT 2013

Immunogenicity and protective capacity of a virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice.

PloS one

Kamphuis T,Meijerhof T,Stegmann T,Lederhofer J,Wilschut J,de Haan A

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Wed Sep 19 00:00:00 EDT 2012

Identification of regulatory functions for 4-1BB and 4-1BBL in myelopoiesis and the development of dendritic cells.

Nature immunology

Lee SW,Park Y,So T,Kwon BS,Cheroutre H,Mittler RS,Croft M

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2008

Region-specific expression of immunoregulatory proteins on microglia in the healthy CNS.

Glia

de Haas AH,Boddeke HW,Biber K

Published figure using CD86 (B7-2) monoclonal antibody (Product # 47-0862-82) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD86; early T cell costimulatory molecule-1; Early T-cell co-stimulatory molecule 1; Early T-cell costimulatory molecule 1; ETC-1; MGC34413; T-lymphocyte activation antigen CD86

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Gene Aliases: B7; B7-2; B7.2; B70; Cd28l2; Cd86; CLS1; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2

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UniProt ID: (Mouse) P42082

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Entrez Gene ID: (Mouse) 12524

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Function(s)

receptor binding immunoglobulin receptor superfamily

Process(es)

toll-like receptor signaling pathway immune system process negative regulation of T cell anergy positive regulation of T cell proliferation positive regulation of activated T cell proliferation B cell activation defense response to virus cellular response to lipopolysaccharide

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CD86 (B7-2) Monoclonal Antibody (GL1), APC-eFluor™ 780, eBioscience™

CD86 (B7-2) Antibody (47-0862-82) in Flow

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