Invitrogen

CD86 (B7-2) Monoclonal Antibody (GL1), Super Bright™ 645, eBioscience™

CD86 (B7-2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 79

CD86 (B7-2) Antibody (64-0862-82) in Flow

Staining of unstimulated (left) or 3-day LPS-stimulated mouse splenocytes (right) with 0.25 µg of Rat IgG2a K Isotype Control Super Bright 645 (Product # 64-4321-82) (blue histogram) or 0.25 µg of Anti-Mouse CD86 (B7-2) Super Bright 645 (purple histogram). Viable cells in the lymphocyte gate, as determined by 7-AAD (Product # 00-6993-50), were used for analysis.

CD86 (B7-2) Antibody in Immunocytochemistry (ICC/IF)

CD86 (B7-2) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

64-0862-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 63 publications 63 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

GL1

Conjugate

Super Bright™ 645 Super Bright™ 645 Super Bright™ 645

Excitation/Emission Max

414/645 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2688123

Product Specific Information

Description: The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.

Applications Reported: This GL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This GL1 antibody has been tested by flow cytometric analysis. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

TLR4 is one of the receptors for Chikungunya virus envelope protein E2 and regulates virus induced pro-inflammatory responses in host macrophages.

Frontiers in immunology

Mahish C,De S,Chatterjee S,Ghosh S,Keshry SS,Mukherjee T,Khamaru S,Tung KS,Subudhi BB,Chattopadhyay S,Chattopadhyay S

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Thu May 18 00:00:00 EDT 2023

Carvacrol protects mice against LPS-induced sepsis and attenuates inflammatory response in macrophages by modulating the ERK1/2 pathway.

Scientific reports

Yan C,Kuang W,Jin L,Wang R,Niu L,Xie C,Ding J,Liao Y,Wang L,Wan H,Ma G

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Aug 07 00:00:00 EDT 2023

TREM2 expression promotes liver and peritoneal M2 macrophage polarization in mice infected with Schistosoma japonicum.

Journal of cellular and molecular medicine

Zhu D,Huang M,Shen P,Zhang B,Chen G,Chen J,Duan L,Duan Y

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

A platinum@polymer-catechol nanobraker enables radio-immunotherapy for crippling melanoma tumorigenesis, angiogenesis, and radioresistance.

Bioactive materials

Li W,Yan J,Tian H,Li B,Wang G,Sang W,Zhang Z,Zhang X,Dai Y

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

BushenHuoxue decoction suppresses M1 macrophage polarization and prevents LPS induced inflammatory bone loss by activating AMPK pathway.

Heliyon

Chen S,Tao L,Zhu F,Wang Z,Zhuang Q,Li Y,Yang Y,Feng C,Shi H,Shi J,Zhu L,Xiao L,Geng D,Wang Z

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

CD8α+ dendritic cells potentiate antitumor and immune activities against murine ovarian cancers.

Scientific reports

Lee SW,Lee H,Lee KW,Kim MJ,Kang SW,Lee YJ,Kim H,Kim YM

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

Acinetobacter baumannii outer membrane protein A induces autophagy in bone marrow-derived dendritic cells involving the PI3K/mTOR pathway.

Immunity, inflammation and disease

Tan H,Cao L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Exosomal Lnc NEAT1 from endothelial cells promote bone regeneration by regulating macrophage polarization via DDX3X/NLRP3 axis.

Journal of nanobiotechnology

Chen Y,Wu Y,Guo L,Yuan S,Sun J,Zhao K,Wang J,An R

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Mar 20 00:00:00 EDT 2023

Role of low-intensity pulsed ultrasound in regulating macrophage polarization to accelerate tendon-bone interface repair.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Xu Z,Li S,Wan L,Hu J,Lu H,Zhang T

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

BMP-2 functional polypeptides relieve osteolysis via bi-regulating bone formation and resorption coupled with macrophage polarization.

NPJ Regenerative medicine

Wang J,Xue Y,Wang Y,Liu C,Hu S,Zhao H,Gu Q,Yang H,Huang L,Zhou X,Shi Q

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Thu Feb 09 00:00:00 EST 2023

Oxygen-carrying nanoplatform to reprogram tumor immunosuppressive microenvironment and enhance photothermal-immunotherapy.

Materials today. Bio

Huang J,Leng X,Jiang T,Xu L,Zheng J,Fang M,Wang J,Wang Z,Zhang L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Nanoplateletsomes restrain metastatic tumor formation through decoy and active targeting in a preclinical mouse model.

Acta pharmaceutica Sinica. B

Zhang L,Zhu Y,Wei X,Chen X,Li Y,Zhu Y,Xia J,Huang Y,Huang Y,Wang J,Pang Z

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Enhanced uptake and anti-maturation effect of celastrol-loaded mannosylated liposomes on dendritic cells for psoriasis treatment.

Acta pharmaceutica Sinica. B

Xi L,Lin Z,Qiu F,Chen S,Li P,Chen X,Wang Z,Zheng Y

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2022

Enhancing arginase 2 expression using target site blockers as a strategy to modulate macrophage phenotype.

Molecular therapy. Nucleic acids

De Santi C,Nally FK,Afzal R,Duffy CP,Fitzsimons S,Annett SL,Robson T,Dowling JK,Cryan SA,McCoy CE

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Tue Sep 13 00:00:00 EDT 2022

Oncolytic Zika virus promotes intratumoral T cell infiltration and improves immunotherapy efficacy in glioblastoma.

Molecular therapy oncolytics

Chen L,Zhou C,Chen Q,Shang J,Liu Z,Guo Y,Li C,Wang H,Ye Q,Li X,Zu S,Li F,Xia Q,Zhou T,Li A,Wang C,Chen Y,Wu A,Qin C,Man J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Thu Mar 17 00:00:00 EDT 2022

G9a promotes inflammation in Streptococcus pneumoniae induced pneumonia mice by stimulating M1 macrophage polarization and H3K9me2 methylation in FOXP1 promoter region.

Annals of translational medicine

Li Y,Li G,Zhang L,Li Y,Zhao Z

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2022

Intranasal Immunization With a c-di-GMP-Adjuvanted Acellular Pertussis Vaccine Provides Superior Immunity Against Bordetella pertussis in a Mouse Model.

Frontiers in immunology

Jiang W,Wang X,Su Y,Cai L,Li J,Liang J,Gu Q,Sun M,Shi L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Tue May 03 00:00:00 EDT 2022

Meningeal lymphatic drainage promotes T cell responses against Toxoplasma gondii but is dispensable for parasite control in the brain.

eLife

Kovacs MA,Cowan MN,Babcock IW,Sibley LA,Still K,Batista SJ,Labuzan SA,Sethi I,Harris TH

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Wed Dec 21 00:00:00 EST 2022

MicroRNA-382 Promotes M2-Like Macrophage via the SIRP-α/STAT3 Signaling Pathway in Aristolochic Acid-Induced Renal Fibrosis.

Frontiers in immunology

Wang X,Jia P,Ren T,Zou Z,Xu S,Zhang Y,Shi Y,Bao S,Li Y,Fang Y,Ding X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Fri May 20 00:00:00 EDT 2022

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Chromomycin A₅ induces bona fide immunogenic cell death in melanoma.

Frontiers in immunology

Florêncio KGD,Edson EA,Fernandes KSDS,Luiz JPM,Pinto FDCL,Pessoa ODL,Cunha FQ,Machado-Neto JA,Wilke DV

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Tue Nov 29 00:00:00 EST 2022

In situ generation of micrometer-sized tumor cell-derived vesicles as autologous cancer vaccines for boosting systemic immune responses.

Nature communications

Guo Y,Wang SZ,Zhang X,Jia HR,Zhu YX,Zhang X,Gao G,Jiang YW,Li C,Chen X,Wu SY,Liu Y,Wu FG

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Targeting dendritic cells with TLR-2 ligand-coated nanoparticles loaded with Mycobacterium tuberculosis epitope induce antituberculosis immunity.

The Journal of biological chemistry

Kumar Das D,Zafar MA,Nanda S,Singh S,Lamba T,Bashir H,Singh P,Maurya SK,Nadeem S,Sehrawat S,Bhalla V,Agrewala JN

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Intracellular osteopontin protects from autoimmunity-driven lymphoma development inhibiting TLR9-MYD88-STAT3 signaling.

Molecular cancer

Rizzello C,Cancila V,Sangaletti S,Botti L,Ratti C,Milani M,Dugo M,Bertoni F,Tripodo C,Chiodoni C,Colombo MP

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Dec 12 00:00:00 EST 2022

Dental pulp stem cell-derived exosomes suppress M1 macrophage polarization through the ROS-MAPK-NFκB P65 signaling pathway after spinal cord injury.

Journal of nanobiotechnology

Liu C,Hu F,Jiao G,Guo Y,Zhou P,Zhang Y,Zhang Z,Yi J,You Y,Li Z,Wang H,Zhang X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Wed Feb 02 00:00:00 EST 2022

Self-assembled traditional Chinese nanomedicine modulating tumor immunosuppressive microenvironment for colorectal cancer immunotherapy.

Theranostics

Mao Q,Min J,Zeng R,Liu H,Li H,Zhang C,Zheng A,Lin J,Liu X,Wu M

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Thu Sep 29 00:00:00 EDT 2022

Role of circular RNA cdr1as in modulation of macrophage phenotype.

Life sciences

Gonzalez C,Cimini M,Cheng Z,Benedict C,Wang C,Trungcao M,Mallaredy V,Rajan S,Garikipati VNS,Kishore R

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2022

A bilirubin-derived nanomedicine attenuates the pathological cascade of pulmonary fibrosis.

Biomaterials

Keum H,Kim D,Kim J,Kim TW,Whang CH,Jung W,Jon S

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Loss of p53 in mesenchymal stem cells promotes alteration of bone remodeling through negative regulation of osteoprotegerin.

Cell death and differentiation

Velletri T,Huang Y,Wang Y,Li Q,Hu M,Xie N,Yang Q,Chen X,Chen Q,Shou P,Gan Y,Candi E,Annicchiarico-Petruzzelli M,Agostini M,Yang H,Melino G,Shi Y,Wang Y

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

CD11b Deficiency Exacerbates Methicillin-Resistant Staphylococcus aureus-Induced Sepsis by Upregulating Inflammatory Responses of Macrophages.

Immune network

Sim H,Jeong D,Kim HI,Pak S,Thapa B,Kwon HJ,Lee K

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Pseudomonas aeruginosa PcrV Enhances the Nitric Oxide-Mediated Tumoricidal Activity of Tumor-Associated Macrophages via a TLR4/PI3K/AKT/mTOR-Glycolysis-Nitric Oxide Circuit.

Frontiers in oncology

Yu H,Bai Y,Qiu J,He X,Xiong J,Dai Q,Wang X,Li Y,Sheng H,Xin R,Jiang L,Li Q,Li D,Zhang H,Zhang L,Chen Q,Peng J,Hu X,Zhang K

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Wed Dec 15 00:00:00 EST 2021

Prostaglandin E2 serves a dual role in regulating the migration of dendritic cells.

International journal of molecular medicine

Diao G,Huang J,Zheng X,Sun X,Tian M,Han J,Guo J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation.

World journal of stem cells

Luo ZW,Sun YY,Lin JR,Qi BJ,Chen JW

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Fri Nov 26 00:00:00 EST 2021

Altered Corneal Epithelial Dendritic Cell Morphology and Phenotype Following Acute Exposure to Hyperosmolar Saline.

Investigative ophthalmology & visual science

Senthil K,Jiao H,Downie LE,Chinnery HR

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Immunocytochemistry

Mon Feb 01 00:00:00 EST 2021

Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat-treated tumor cell lysates enhance antitumor activity in murine lung cancer.

Oncology letters

Chen H,Tan J,Li X,Li H,Wu W,Wu Y,Zhang J,Gu L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.

Cells

Jhan MK,Chen CL,Shen TJ,Tseng PC,Wang YT,Satria RD,Yu CY,Lin CF

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Nov 15 00:00:00 EST 2021

Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen.

Cell reports

Boccasavia VL,Bovolenta ER,Villanueva A,Borroto A,Oeste CL,van Santen HM,Prieto C,Alonso-López D,Diaz-Muñoz MD,Batista FD,Alarcón B

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Immunocytochemistry

Tue Mar 16 00:00:00 EDT 2021

Modulation of lactate-lysosome axis in dendritic cells by clotrimazole potentiates antitumor immunity.

Journal for immunotherapy of cancer

Wang Z,Xu F,Hu J,Zhang H,Cui L,Lu W,He W,Wang X,Li M,Zhang H,Xiong W,Xie C,Liu Y,Zhou P,Liu J,Huang P,Qin XF,Xia X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

A two-adjuvant multiantigen candidate vaccine induces superior protective immune responses against SARS-CoV-2 challenge.

Cell reports

Jiang W,Shi L,Cai L,Wang X,Li J,Li H,Liang J,Gu Q,Ji G,Li J,Liu L,Sun M

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Tue Dec 14 00:00:00 EST 2021

Theranostic near-infrared-IIb emitting nanoprobes for promoting immunogenic radiotherapy and abscopal effects against cancer metastasis.

Nature communications

Li H,Wang M,Huang B,Zhu SW,Zhou JJ,Chen DR,Cui R,Zhang M,Sun ZJ

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Thu Dec 09 00:00:00 EST 2021

Inhibition of TLR4 signaling protects mice from sensory and motor dysfunction in an animal model of autoimmune peripheral neuropathy.

Journal of neuroinflammation

Oladiran O,Shi XQ,Yang M,Fournier S,Zhang J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Mar 22 00:00:00 EDT 2021

Adrenomedullin 2 improves bone regeneration in type 1 diabetic rats by restoring imbalanced macrophage polarization and impaired osteogenesis.

Stem cell research & therapy

Wang F,Kong L,Wang W,Shi L,Wang M,Chai Y,Xu J,Kang Q

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Marine-inspired molecular mimicry generates a drug-free, but immunogenic hydrogel adhesive protecting surgical anastomosis.

Bioactive materials

Huang J,Jiang Y,Liu Y,Ren Y,Xu Z,Li Z,Zhao Y,Wu X,Ren J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Cytochrome P450 26A1 Modulates the Polarization of Uterine Macrophages During the Peri-Implantation Period.

Frontiers in immunology

Ji WH,Li DD,Wei DP,Gu AQ,Yang Y,Peng JP

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Thu Feb 10 00:00:00 EST 2022

C-Reactive Protein Suppresses the Th17 Response Indirectly by Attenuating the Antigen Presentation Ability of Monocyte Derived Dendritic Cells in Experimental Autoimmune Encephalomyelitis.

Frontiers in immunology

Shen ZY,Zheng Y,Pecsok MK,Wang K,Li W,Gong MJ,Wu F,Zhang L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway.

Frontiers in immunology

Lv N,Jin S,Liang Z,Wu X,Kang Y,Su L,Dong Y,Wang B,Ma T,Shi L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Feb 28 00:00:00 EST 2022

E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions.

International journal of oncology

Huang J,Diao G,Zhang Q,Chen Y,Han J,Guo J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Mesenchymal stem cell-secreted extracellular vesicles carrying TGF-β1 up-regulate miR-132 and promote mouse M2 macrophage polarization.

Journal of cellular and molecular medicine

Wang Y,Han B,Wang Y,Wang C,Zhang H,Xue J,Wang X,Niu T,Niu Z,Chen Y

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Interleukin-9 Inhibits Lung Metastasis of Melanoma through Stimulating Anti-Tumor M1 Macrophages.

Molecules and cells

Park SM,Do-Thi VA,Lee JO,Lee H,Kim YS

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Sun May 31 00:00:00 EDT 2020

Effects of combined treatment with PD‑L1 Ig and CD40L mAb on immune tolerance in the CBA/J x DBA/2 mouse model.

Molecular medicine reports

Li G,Yang L,Li D,Zhang J,Du L,Xia L,Liu Y,Hu W

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Angiotensin II induces RAW264.7 macrophage polarization to the M1‑type through the connexin 43/NF‑κB pathway.

Molecular medicine reports

Wu L,Chen K,Xiao J,Xin J,Zhang L,Li X,Li L,Si J,Wang L,Ma K

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Vaccination with early ferroptotic cancer cells induces efficient antitumor immunity.

Journal for immunotherapy of cancer

Efimova I,Catanzaro E,Van der Meeren L,Turubanova VD,Hammad H,Mishchenko TA,Vedunova MV,Fimognari C,Bachert C,Coppieters F,Lefever S,Skirtach AG,Krysko O,Krysko DV

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Intravenous injection of the oncolytic virus M1 awakens antitumor T cells and overcomes resistance to checkpoint blockade.

Cell death & disease

Liu Y,Cai J,Liu W,Lin Y,Guo L,Liu X,Qin Z,Xu C,Zhang Y,Su X,Deng K,Yan G,Liang J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Sat Dec 12 00:00:00 EST 2020

MiR-103 protects from recurrent spontaneous abortion via inhibiting STAT1 mediated M1 macrophage polarization.

International journal of biological sciences

Zhu X,Liu H,Zhang Z,Wei R,Zhou X,Wang Z,Zhao L,Guo Q,Zhang Y,Chu C,Wang L,Li X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Fri Nov 12 00:00:00 EST 2021

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Restructures the Immune Contexture to Improve Checkpoint Blockade Efficacy.

Biomedicines

Ebelt ND,Zuniga E,Marzagalli M,Zamloot V,Blazar BR,Salgia R,Manuel ER

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Restructures the Immune Contexture to Improve Checkpoint Blockade Efficacy.

Biomedicines

Ebelt ND,Zuniga E,Marzagalli M,Zamloot V,Blazar BR,Salgia R,Manuel ER

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

Lactobacillus reuteri 5454 and Bifidobacterium animalis ssp. lactis 5764 improve colitis while differentially impacting dendritic cells maturation and antimicrobial responses.

Scientific reports

Hrdý J,Alard J,Couturier-Maillard A,Boulard O,Boutillier D,Delacre M,Lapadatescu C,Cesaro A,Blanc P,Pot B,Ryffel B,Chamaillard M,Grangette C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Tue Mar 24 00:00:00 EDT 2020

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2020

Polyanhydride Nanoparticles Induce Low Inflammatory Dendritic Cell Activation Resulting in CD8⁺ T Cell Memory and Delayed Tumor Progression.

International journal of nanomedicine

Darling R,Senapati S,Christiansen J,Liu L,Ramer-Tait AE,Narasimhan B,Wannemuehler M

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

Epigenetically modulated FOXM1 suppresses dendritic cell maturation in pancreatic cancer and colon cancer.

Molecular oncology

Zhou Z,Chen H,Xie R,Wang H,Li S,Xu Q,Xu N,Cheng Q,Qian Y,Huang R,Shao Z,Xiang M

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

Arp2/3 complex-driven spatial patterning of the BCR enhances immune synapse formation, BCR signaling and B cell activation.

eLife

Bolger-Munro M,Choi K,Scurll JM,Abraham L,Chappell RS,Sheen D,Dang-Lawson M,Wu X,Priatel JJ,Coombs D,Hammer JA,Gold MR

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Jun 03 00:00:00 EDT 2019

Efficient oral vaccination by bioengineering virus-like particles with protozoan surface proteins.

Nature communications

Serradell MC,Rupil LL,Martino RA,Prucca CG,Carranza PG,Saura A,Fernández EA,Gargantini PR,Tenaglia AH,Petiti JP,Tonelli RR,Reinoso-Vizcaino N,Echenique J,Berod L,Piaggio E,Bellier B,Sparwasser T,Klatzmann D,Luján HD

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Jan 21 00:00:00 EST 2019

Cannabinoid 2 receptor attenuates inflammation during skin wound healing by inhibiting M1 macrophages rather than activating M2 macrophages.

Journal of inflammation (London, England)

Du Y,Ren P,Wang Q,Jiang SK,Zhang M,Li JY,Wang LL,Guan DW

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Immunohistochemistry

Wed Mar 30 00:00:00 EDT 2022

Targeting macrophage and microglia activation with colony stimulating factor 1 receptor inhibitor is an effective strategy to treat injury-triggered neuropathic pain.

Molecular pain

Lee S,Shi XQ,Fan A,West B,Zhang J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Oct 22 00:00:00 EDT 2018

Paeoniflorin Ameliorates Experimental Autoimmune Encephalomyelitis via Inhibition of Dendritic Cell Function and Th17 Cell Differentiation.

Scientific reports

Zhang H,Qi Y,Yuan Y,Cai L,Xu H,Zhang L,Su B,Nie H

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Mon Feb 06 00:00:00 EST 2017

Tumor-derived exosomes educate dendritic cells to promote tumor metastasis via HSP72/HSP105-TLR2/TLR4 pathway.

Oncoimmunology

Shen Y,Guo D,Weng L,Wang S,Ma Z,Yang Y,Wang P,Wang J,Cai Z

Published figure using CD86 (B7-2) monoclonal antibody (Product # 64-0862-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Bioinformatics

Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD86; early T cell costimulatory molecule-1; Early T-cell co-stimulatory molecule 1; Early T-cell costimulatory molecule 1; ETC-1; MGC34413; T-lymphocyte activation antigen CD86

View more View less

Gene Aliases: B7; B7-2; B7.2; B70; Cd28l2; Cd86; CLS1; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2

View more View less

UniProt ID: (Mouse) P42082

View more View less

Entrez Gene ID: (Mouse) 12524

View more View less

Function(s)

receptor binding immunoglobulin receptor superfamily

Process(es)

toll-like receptor signaling pathway immune system process negative regulation of T cell anergy positive regulation of T cell proliferation positive regulation of activated T cell proliferation B cell activation defense response to virus cellular response to lipopolysaccharide

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD86 (B7-2) Monoclonal Antibody (GL1), Super Bright™ 645, eBioscience™

CD86 (B7-2) Antibody (64-0862-82) in Flow

Product Details

64-0862-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!