Invitrogen

CD86 (B7-2) Monoclonal Antibody (IT2.2), eBioscience™

CD86 (B7-2) Antibody in Flow Cytometry (Flow)

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CD86 (B7-2) Antibody (14-0869-82) in Flow

Normal human peripheral blood stained with Anti-Human CD86 (B7-2) Purified followed by Anti-Mouse IgG FITC (Product # 11-4011-85. Appropriate isotype controls were used (open histogram). Cells in the monocyte gate were analyzed.

CD86 (B7-2) Antibody in Immunocytochemistry (ICC/IF)

CD86 (B7-2) Antibody in Neutralization (Neu)

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Product Details

14-0869-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 21 publications 21 publications

Neutralization (Neu)

Assay-Dependent

View 2 publications 2 publications

Functional Assay (Functional)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

IT2.2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467372

Product Specific Information

Description: The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.

Applications Reported: The IT2.2 antibody has been reported for use in flow cytometric analysis. IT2.2 has also been reported in blocking of CD86 in functional studies. (Please use Functional Grade purified IT2.2, Product # 16-0869, in functional assays).

Applications Tested: The IT2.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Germline Neurofibromin 1 mutation enhances the anti-tumour immune response and decreases juvenile myelomonocytic leukaemia tumourigenicity.

British journal of haematology

Wang W,Li X,Qin X,Miao Y,Zhang Y,Li S,Yao R,Yang Y,Yu L,Zhu H,Song L,Mao S,Wang X,Chen J,Feng H,Li Y

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Engineered Human Dendritic Cell Exosomes as Effective Delivery System for Immune Modulation.

International journal of molecular sciences

Elsayed R,Elashiry M,Tran C,Yang T,Carroll A,Liu Y,Hamrick M,Cutler CW

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Tue Jul 11 00:00:00 EDT 2023

Activation of the JNK/COX-2/HIF-1α axis promotes M1 macrophage via glycolytic shift in HIV-1 infection.

Life science alliance

Zhang J,Yuan Z,Li X,Wang F,Wei X,Kang Y,Mo C,Jiang J,Liang H,Ye L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2023

SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophages.

PeerJ

Thaweerattanasinp T,Wanitchang A,Saenboonrueng J,Srisutthisamphan K,Wanasen N,Sungsuwan S,Jongkaewwattana A,Chailangkarn T

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2023

M1 macrophage-derived exosomes and their key molecule lncRNA HOTTIP suppress head and neck squamous cell carcinoma progression by upregulating the TLR5/NF-κB pathway.

Cell death & disease

Jiang H,Zhou L,Shen N,Ning X,Wu D,Jiang K,Huang X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Thu Feb 24 00:00:00 EST 2022

TcpC Inhibits M1 but Promotes M2 Macrophage Polarization via Regulation of the MAPK/NF-κB and Akt/STAT6 Pathways in Urinary Tract Infection.

Cells

Fang J,Ou Q,Wu B,Li S,Wu M,Qiu J,Cen N,Hu K,Che Y,Ma Y,Pan J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Sun Aug 28 00:00:00 EDT 2022

Glycerol monolaurate inhibition of human B cell activation.

Scientific reports

Fosdick MG,Loftus S,Phillips I,Zacharias ZR,Houtman JCD

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Fri Aug 05 00:00:00 EDT 2022

Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19.

Immunity

Szabo PA,Dogra P,Gray JI,Wells SB,Connors TJ,Weisberg SP,Krupska I,Matsumoto R,Poon MML,Idzikowski E,Morris SE,Pasin C,Yates AJ,Ku A,Chait M,Davis-Porada J,Guo XV,Zhou J,Steinle M,Mackay S,Saqi A,Baldwin MR,Sims PA,Farber DL

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain.

Nature chemical biology

Shukla S,Ying W,Gray F,Yao Y,Simes ML,Zhao Q,Miao H,Cho HJ,González-Alonso P,Winkler A,Lund G,Purohit T,Kim E,Zhang X,Ray JM,He S,Nikolaidis C,Ndoj J,Wang J,Jaremko Ł,Jaremko M,Ryan RJH,Guzman ML,Grembecka J,Cierpicki T

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2.

PLoS pathogens

Shrestha P,Davis DA,Jaeger HK,Stream A,Aisabor AI,Yarchoan R

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Neutralization

Fri Jan 01 00:00:00 EST 2021

IL-10^-/- Enhances DCs Immunity Against Chlamydia psittaci Infection via OX40L/NLRP3 and IDO/Treg Pathways.

Frontiers in immunology

Li Q,Li X,Quan H,Wang Y,Qu G,Shen Z,He C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Dendritic Cell Maturation Regulates TSPAN7 Function in HIV-1 Transfer to CD4⁺ T Lymphocytes.

Frontiers in cellular and infection microbiology

Perot BP,García-Paredes V,Luka M,Ménager MM

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Wed Mar 17 00:00:00 EDT 2021

Reshaping of the Dendritic Cell Chromatin Landscape and Interferon Pathways during HIV Infection.

Cell host & microbe

Johnson JS,Lucas SY,Amon LM,Skelton S,Nazitto R,Carbonetti S,Sather DN,Littman DR,Aderem A

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Wed Mar 14 00:00:00 EDT 2018

AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.

Oncology letters

Xiu W,Ma J,Lei T,Zhang M

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70.

Oncology letters

Zhang F,Zhu Y,Fan G,Hu S

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Ginsenoside Rg3 Mitigates Atherosclerosis Progression in Diabetic apoE-/- Mice by Skewing Macrophages to the M2 Phenotype.

Frontiers in pharmacology

Guo M,Xiao J,Sheng X,Zhang X,Tie Y,Wang L,Zhao L,Ji X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.

Bioscience reports

Zhong Y,Yi C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Pentraxin-3 regulates the inflammatory activity of macrophages.

Biochemistry and biophysics reports

Shiraki A,Kotooka N,Komoda H,Hirase T,Oyama JI,Node K

Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0869-82) in Immunocytochemistry

Tue Mar 01 00:00:00 EST 2016

One, two or three? Probing the stoichiometry of membrane proteins by single-molecule localization microscopy.

Scientific reports

Fricke F,Beaudouin J,Eils R,Heilemann M

14-0869 was used in Flow cytometry/Cell sorting to demonstrate the use of a single-molecule counting strategy in exploring stoichiometry of membrane proteins.

Fri Sep 11 00:00:00 EDT 2015

CD137L-stimulated dendritic cells are more potent than conventional dendritic cells at eliciting cytotoxic T-cell responses.

Oncoimmunology

Harfuddin Z,Kwajah S,Chong Nyi Sim A,Macary PA,Schwarz H

14-0869 was used in Flow cytometry/Cell sorting to develop improved dendritic cell-based immunotherapy using CD137 signalling.

Fri Nov 01 00:00:00 EDT 2013

Lung dendritic cell expression of maturation molecules increases with worsening chronic obstructive pulmonary disease.

American journal of respiratory and critical care medicine

Freeman CM,Martinez FJ,Han MK,Ames TM,Chensue SW,Todt JC,Arenberg DA,Meldrum CA,Getty C,McCloskey L,Curtis JL

14-0869 was used in Flow cytometry/Cell sorting to determine the expression of maturation molecules by individual DC subsets in relationship to COPD stage.

Tue Dec 15 00:00:00 EST 2009

Severe loss of invariant NKT cells exhibiting anti-HTLV-1 activity in patients with HTLV-1-associated disorders.

Blood

Azakami K,Sato T,Araya N,Utsunomiya A,Kubota R,Suzuki K,Hasegawa D,Izumi T,Fujita H,Aratani S,Fujii R,Yagishita N,Kamijuku H,Kanekura T,Seino K,Nishioka K,Nakajima T,Yamano Y

14-0869 was used in Flow cytometry/Cell sorting to assess the contribution of iNKT cells to the immune defence against human T-lymphotropic virus type 1 and associated disorders.

Thu Oct 08 00:00:00 EDT 2009

Renal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates.

The Journal of clinical investigation

Bashuda H,Kimikawa M,Seino K,Kato Y,Ono F,Shimizu A,Yagita H,Teraoka S,Okumura K

14-0869 was used in Blocking experiments to test whether anergic T cells generated ex vivo are immunosuppressive in nonhuman primates, showing that renal allograft rejection is prevented by adoptive transfer of these cells.

Fri Jul 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2; B70; BU63; CD28 antigen ligand 2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; FUN-1; MGC34413; T-lymphocyte activation antigen CD86

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Gene Aliases: B7-2; B7.2; B70; CD28LG2; CD86; LAB72

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UniProt ID: (Human) P42081

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Entrez Gene ID: (Human) 942

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Function(s)

virus receptor activity receptor activity receptor binding protein binding coreceptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity immunoglobulin receptor superfamily

Process(es)

response to yeast adaptive immune response T cell proliferation involved in immune response negative regulation of T cell anergy immune response cell-cell signaling aging positive regulation of cell proliferation regulation of phosphatidylinositol 3-kinase signaling T cell costimulation toll-like receptor 3 signaling pathway response to interferon-gamma positive regulation of activated T cell proliferation T cell activation B cell activation response to drug myeloid dendritic cell differentiation positive regulation of lymphotoxin A biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-4 biosynthetic process positive regulation of T-helper 2 cell differentiation positive regulation of transcription, DNA-templated viral entry into host cell phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling defense response to virus cellular response to lipopolysaccharide cellular response to metal ion cellular response to cytokine stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD86 (B7-2) Monoclonal Antibody (IT2.2), eBioscience™

CD86 (B7-2) Antibody (14-0869-82) in Flow

Product Details

14-0869-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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